Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,169	0	T	G	44.9%	92.3 / 28.0	78	L20V (TTG→GTG)	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (15/28);  new base G (35/0);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 1.77e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAC  >  NC_000913/312026‑312317                                                                                                                                                |                                                                                                                                                     ggATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATg                                                                                                                                                 >  1:464677/1‑149 (MQ=255) ggATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGcc                                                                                                                                               <  2:601896/151‑1 (MQ=255)   aTCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTAACAACAGATTTACCACAGTTATCAACTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGGCAg                                                                                                                                             <  2:906719/151‑1 (MQ=255)     cAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGc                                                                                                                                            <  1:192108/150‑1 (MQ=255)     cAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGc                                                                                                                                            >  2:867321/1‑150 (MQ=255)      aGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTCAAAGAGGGTTGATGCCCGCAt                                                                                                                                          >  2:201640/1‑151 (MQ=255)           gTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAgg                                                                                                                                     >  2:305948/1‑151 (MQ=255)           gTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAgg                                                                                                                                     >  1:147865/1‑151 (MQ=255)            ttCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAggg                                                                                                                                    >  1:100847/1‑151 (MQ=255)              ccTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGc                                                                                                                                  <  2:1086040/151‑1 (MQ=255)                tgtgGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAgggggg                                                                                                                                 >  2:388644/1‑148 (MQ=255)                 gtgGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGa                                                                                                                                <  2:922720/150‑1 (MQ=255)                   ggCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGaa                                                                                                                               >  2:185106/1‑149 (MQ=255)                     cGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTg                                                                                                                           >  2:487845/1‑151 (MQ=255)                      gTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTgg                                                                                                                          >  2:372455/1‑151 (MQ=255)                        aaaCGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTgg                                                                                                                          >  1:700096/1‑149 (MQ=255)                          aCGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTca                                                                                                                       >  1:1194775/1‑150 (MQ=255)                            gTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGGCAGCCTACAAGGGCGAAGCTGGTcaa                                                                                                                      >  1:1190154/1‑149 (MQ=255)                              ttttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCCGTCTAAAAGAGGGGTGATGCCGGCATACAGGGGCGAAGCTGGTcaaca                                                                                                                    >  1:313537/1‑149 (MQ=255)                              ttttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTcaacaa                                                                                                                   <  1:159318/150‑1 (MQ=255)                              ttttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTcaaca                                                                                                                    >  1:1004893/1‑149 (MQ=255)                              ttttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGGATACAGGGGCGAAGCTGGTcaaca                                                                                                                    >  2:983514/1‑149 (MQ=255)                                  aTGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCa                                                                                                               <  1:691495/150‑1 (MQ=255)                                    gTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAAc                                                                                                             >  1:1149632/1‑150 (MQ=255)                                     ttAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCCGCATACAGGGGCGAAGCTGGTCAACAAGTCAAc                                                                                                             >  1:1051176/1‑149 (MQ=255)                                            cAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATa                                                                                                    <  2:1109048/151‑1 (MQ=255)                                               cAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATg                                                                                                 <  2:70181/151‑1 (MQ=255)                                               cAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATaa                                                                                                   >  2:79549/1‑149 (MQ=255)                                                aGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTTAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGGAGCTGGTCAACAAGACAACATTAAGATAATgg                                                                                                >  2:915107/1‑151 (MQ=255)                                                aGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGGTCAGGCTAACAAAGGGTTTGTTCCCGCCTTCAGGGGCGAAGGTGGCCAAAAAGGCAACATTAAGATaaag                                                                                                 >  2:586196/1‑148 (MQ=255)                                                  aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGa                                                                                               <  2:421954/150‑1 (MQ=255)                                                     tACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGGCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGGCAACAAGTCAACATTAAGATAATGGAatat                                                                                           >  1:1170504/1‑151 (MQ=255)                                                       ccACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCATCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAtt                                                                                          <  1:911990/150‑1 (MQ=255)                                                       ccACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGATCAAAAGAGGGGTGATGCCAGGATACAGGGGGGAAGCTGGTCCACAAATCAAAATTAAAATAATGGAATAttt                                                                                         >  2:1081139/1‑150 (MQ=255)                                                        cacaTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTTAGTCTAAAAAAGGGGTGATGCCAGCATACAGGGGCGGAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAtt                                                                                          >  2:678743/1‑149 (MQ=255)                                                           aTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCgg                                                                                       >  1:367659/1‑149 (MQ=255)                                                             ttATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTTAGTCTAAAAGAGGGGTGATGGCCAAAAAAAGGGGGGAAGCTGGTCAACAAATCCACCTTAAGAAAATGGAATATTCGgag                                                                                     >  2:290252/1‑149 (MQ=255)                                                                 catcTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGGGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGaaa                                                                                >  2:612166/1‑150 (MQ=255)                                                                     ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTTAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGGGGAGCTGGTCAACAAATCAACATTAAGATAATGGAAAATTCGGGGAGAAAAGTc                                                                            >  2:437140/1‑150 (MQ=255)                                                                        aCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGGTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAgg                                                                         >  2:462943/1‑150 (MQ=255)                                                                        aCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAAAGGGGTGATGCCAGCCTACAGGGGCGGAGCTTGTCAACAAGTCAACATTTAGATAATGGAATATTCGGAGAGAAACGTCAgg                                                                         >  1:349586/1‑150 (MQ=255)                                                                         cAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTTAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGGTGGGCAACAAGTCAACATTAAGATAAAGGAATATTCGGAGAGGAACGTCa                                                                           >  2:888686/1‑147 (MQ=255)                                                                           gTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTTAATCTAAAAGAGGGGTGATGCCAGCATAAAGGGGCGAAGCTGGTCAAAAAATCAACATTAAGATAATGGAATATTCGGGGAGAAAAGACAggta                                                                       >  2:1016753/1‑147 (MQ=255)                                                                             ttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGtt                                                                    >  2:105174/1‑150 (MQ=255)                                                                               gAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTgg                                                                  <  2:189968/150‑1 (MQ=255)                                                                                 gTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGcc                                                                <  2:711244/150‑1 (MQ=255)                                                                                 gTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTAAGTCTAAAAAAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGcc                                                                >  2:519519/1‑150 (MQ=255)                                                                                   gAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCa                                                             <  1:34955/151‑1 (MQ=255)                                                                                    aaaaTGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCa                                                             >  1:1064300/1‑150 (MQ=255)                                                                                         gCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGaa                                                       <  1:726496/151‑1 (MQ=255)                                                                                            ggCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAAAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGGCAAAAAGTCAACAATAAGATAATGGAAAATTCGGAGAGAAACGTCAGGCAGGTGGCCTCAAAAGaa                                                       >  1:436350/1‑148 (MQ=255)                                                                                               tGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                  >  2:837604/1‑150 (MQ=255)                                                                                                  gccgcAAAGGGAGGAGACTGAGTGGCGTGTTTAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGGAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGgaaga                                               >  2:476091/1‑150 (MQ=255)                                                                                                    cgcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAata                                            <  1:186302/151‑1 (MQ=255)                                                                                                      cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAatata                                          <  2:912320/151‑1 (MQ=255)                                                                                                       aaaGGGAGGAGACTGAGTGGCGTGTTCAGTCTCAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGGAAGAat                                             >  2:996022/1‑147 (MQ=255)                                                                                                       aaaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAatata                                          >  2:225356/1‑150 (MQ=255)                                                                                                       aaaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACCTCAGGCAGTTGGCCTCAAATGAACAGgaaga                                               >  1:415289/1‑145 (MQ=255)                                                                                                       aaaGGGAGGAGACTGAGTGGCGTGTTCAGGCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAatata                                          >  1:623145/1‑150 (MQ=255)                                                                                                        aaGGGAGGAGACTGAGTGGCGTGCTCAGTCTAAAAGAAGGGTGATGCCAGCATACAGGGGGGGAGCTGGTCAACAAAGCAACCTTAAAATAATGGGAAATTCGGAGAGAAACGTCAGGGAGTTGGCCCCCAATGAAAAGGAAGAatatat                                         >  2:669656/1‑150 (MQ=255)                                                                                                          gggAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATccc                                      <  1:289313/151‑1 (MQ=255)                                                                                                           ggaggaGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATcccc                                     >  2:354455/1‑151 (MQ=255)                                                                                                           ggagcaGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGAATACAGGTGGGAAGCAGGTCAACAACTTAACATTAAAATAATGGAATATTCGGAGACAAACGTCAGGTAGTTGGCCTCAAATGATCTGGATTAATATATc                                        <  2:2275/148‑1 (MQ=255)                                                                                                             aggagACTGAGTGGCGTGTTCAGGCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATcccc                                     >  1:154025/1‑149 (MQ=255)                                                                                                              ggagACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAgg                                  <  1:932568/151‑1 (MQ=255)                                                                                                                agaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGaa                                <  1:568004/151‑1 (MQ=255)                                                                                                                 gaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGaaa                               <  2:1051470/151‑1 (MQ=255)                                                                                                                     gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAAtc                            <  2:510860/150‑2 (MQ=255)                                                                                                                       gTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAAt                         <  1:289150/151‑1 (MQ=255)                                                                                                                        tGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATg                        >  2:360278/1‑151 (MQ=255)                                                                                                                           cGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                     <  2:1190095/151‑1 (MQ=255)                                                                                                                            gtgtTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTgg                    >  2:613121/1‑151 (MQ=255)                                                                                                                                  aGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTAAACAAGACAACATTTAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCGCCAGGAAAATTAATGTTGGTGTtat              >  2:1168846/1‑151 (MQ=255)                                                                                                                                       aaaaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaa            <  1:201645/148‑1 (MQ=255)                                                                                                                                        aaaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac        >  1:1171789/1‑151 (MQ=255)                                                                                                                                        aaaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac        >  2:295536/1‑151 (MQ=255)                                                                                                                                              ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAc  <  1:185108/151‑1 (MQ=255)                                                                                                                                              ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAc  <  2:747617/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAC  >  NC_000913/312026‑312317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑