Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG39.7% 116.4 / 10.2 63V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/35);  new base G (25/0);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.34e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437
                                                                                                                                                     |                                                                                                                                                      
ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTg                                                                                                                                                       <  2:327368/149‑1 (MQ=255)
ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGaa                                                                                                                                                     <  1:113905/151‑1 (MQ=255)
  ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAg                                                                                                                                                   <  1:96949/151‑1 (MQ=255)
   gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGa                                                                                                                                                  <  1:1183440/151‑1 (MQ=255)
    cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGAt                                                                                                                                                 <  2:345319/151‑1 (MQ=255)
     aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTTGGGGGGaa                                                                                                                                                     >  1:1117478/1‑147 (MQ=255)
     aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAg                                                                                                                                                   >  2:274665/1‑148 (MQ=255)
       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATgc                                                                                                                                               <  1:173858/150‑1 (MQ=255)
         gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGGAAGaagggc                                                                                                                                             >  2:1047803/1‑145 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                       <  1:819771/151‑1 (MQ=255)
              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                       <  1:1045479/151‑1 (MQ=255)
                 gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                                      <  1:755494/149‑1 (MQ=255)
                  aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                   <  2:758309/151‑1 (MQ=255)
                  aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                   <  1:783291/151‑1 (MQ=255)
                   cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTTGGGGGGAAGATGCGCGGTAAggg                                                                                                                                     >  1:69885/1‑148 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAGAAGCGCTGGAAgggggggggg                                                                                                                              >  2:788808/1‑144 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑GGGGTGGGGGGGAAGATGGGCGGGAAAGGGAggggg                                                                                                                              >  1:1098331/1‑151 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑AGGGTGGGGTGAAAAATCCGGTGataagg                                                                                                                                     >  1:821836/1‑139 (MQ=255)
                          cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                            <  2:257997/150‑1 (MQ=255)
                          cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                            <  1:440739/150‑1 (MQ=255)
                                 ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑GGGTTGGGGGGAGAGGTGGGGTGCGAGGGGCggtgggggggggg                                                                                                                      >  2:547643/1‑147 (MQ=255)
                                   ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                    <  1:188243/149‑1 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                   <  1:862144/148‑1 (MQ=255)
                                       ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑t                                                                                                             <  2:976516/151‑1 (MQ=255)
                                            aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCg                                                                                                         <  2:1025660/150‑1 (MQ=255)
                                              tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGAAAAGAGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTgggatttc                                                                                                           >  2:546205/1‑140 (MQ=255)
                                               gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGaaa                                                                                                      <  2:1084978/150‑1 (MQ=255)
                                                   aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAAAAGGGCTGGAAAGGGGGGGGGGGGGGGGGGTTGTCATTTCCGaa                                                                                                       >  2:1115335/1‑146 (MQ=255)
                                                     gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCg                                                                                               <  1:881735/151‑1 (MQ=255)
                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGGAAGATGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTGTCA‑TTCCGAAagag                                                                                                   >  1:241032/1‑145 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGCCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑CGGTTGGGGTGGAAGAGGCGCCGAAAGGGGCGGTGGCGGGGGGGGTTGGTA‑TTTCGGAAAAGAGCg                                                                                               >  1:968136/1‑148 (MQ=255)
                                                             cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGAAAGAAGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTGTCA‑TTTCGAAAGAGAGCGGCAGTCt                                                                                        >  1:1016350/1‑150 (MQ=255)
                                                                tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCA‑TTCCGAAAGAGAGGGACAGTCTggg                                                                                     >  1:107329/1‑149 (MQ=255)
                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGa                                                                                     <  2:255890/149‑1 (MQ=255)
                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                   <  2:610473/151‑1 (MQ=255)
                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                 <  1:379411/151‑1 (MQ=255)
                                                                     ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCA‑TTCCGAAAGAGAGGGACAGTCTgg                                                                                      >  1:1011820/1‑144 (MQ=255)
                                                                      gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                              <  2:213441/151‑1 (MQ=255)
                                                                            gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                         <  2:1075810/150‑1 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                       <  1:1047855/149‑1 (MQ=255)
                                                                                 ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   <  1:380283/151‑1 (MQ=255)
                                                                                 ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   <  2:340411/151‑1 (MQ=255)
                                                                                 ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGGAAAATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCA‑TTCCGGAAGAGAGCGACAGGATGGGAGATCGTGGTGTgg                                                                       >  1:491494/1‑147 (MQ=255)
                                                                                   aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCAc                                                                  >  1:1011972/1‑150 (MQ=255)
                                                                                     gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGGAAAATGCGGTGAAAGGGGAGGGGGGGGGGGGGGTTGTCA‑TTCCGGAAGAGAGCGGCAGTCTGGAAGATCGGGGTGTGGCGCAccc                                                                >  2:365223/1‑148 (MQ=255)
                                                                                        cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCA‑TTTCGGAAAAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACt                                                                 >  2:301037/1‑146 (MQ=255)
                                                                                            aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑GGGTTGGGGGGGAAGATGCGCGGAAAGGGCAGGGGGGGGGGGGGTTTGGTT‑TTCCGAAAAAGGGGGAAAGACTGGGAGGTAGCGGAGTGGCGGACTCCCaa                                                            >  2:1136084/1‑147 (MQ=255)
                                                                                             ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑GGGTTGGGGTGTAAGAAGGCCTTAAAGGGCAGGGGGCGGGGGGGGTTGTCG‑TTTCGAAAGAGAGGGCCACTCTCGAAGACTGTGTGGGGGCGCACGCGCAaga                                                          >  2:7414/1‑148 (MQ=255)
                                                                                                 agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                   <  1:1103476/151‑1 (MQ=255)
                                                                                                    gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                                <  1:426328/151‑1 (MQ=255)
                                                                                                       tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTTTCA‑TTCCGAAAGAGAGCGACAAGCTGGAAGATCGTGATGTGGCGCCCTCGCAAGAGAAGGGGATTa                                               >  1:839799/1‑149 (MQ=255)
                                                                                                          ctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGAAAAATGAGCCGTAAAAGCAGGTGGCGGTGGCGCTTGTTT‑TTTCGGAAGGGAGGGAAAATCTGGAAGGACATGATGGGGCGCGCTCGGCAGAGAAGGGGGTTAt                                              >  2:1021752/1‑147 (MQ=255)
                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGCGGGTTGGGGGGAAA‑ATGCGCCCAAAAGGCCGGGGGGGGTGGCGGTTGTCA‑TTCCCAAAAAGGGCGCCAGGCTGGAAGATCGTGGTGTGGGGCACGCGCAAGAGGAAGGGATTAttg                                            >  2:468998/1‑145 (MQ=255)
                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                            <  2:1169517/147‑1 (MQ=255)
                                                                                                             cAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGAATATGgcgc                                         >  1:839005/1‑149 (MQ=255)
                                                                                                               gCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  2:608289/151‑1 (MQ=255)
                                                                                                                ccATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTGTCA‑TTCCGAAAGAGAGGGAAAGGCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGGGTATGGCGCtgggg                                    >  2:222331/1‑149 (MQ=255)
                                                                                                                    ccGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                <  2:314586/151‑1 (MQ=255)
                                                                                                                     cGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                               <  2:35733/151‑1 (MQ=255)
                                                                                                                      gggCGTTGCCGAAGTGGC‑GG‑GGGATGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGTGGGGTTTGTCA‑TTCCGAAAGAGAGAGACAGTCTGGCAGATCGGGATGTGGCGCACTCGCCAAAGAAGGCGGTTATGgcg                                          >  1:520364/1‑139 (MQ=255)
                                                                                                                            tGCCGAAGTGGC‑GG‑TGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGGTTGTCA‑TTTCCGAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCACTCGCAAGAGAAAGCGATTATGGCGCTGGTGGGCAGCCAGAtt                        >  2:40765/1‑151 (MQ=255)
                                                                                                                              ccGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                       <  2:1178247/150‑1 (MQ=255)
                                                                                                                                 aaGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                   <  1:178840/151‑1 (MQ=255)
                                                                                                                                                     tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:1036932/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑