Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,573	T→G	D621A (GAT→GCT)	ptsP ←	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,573	0	T	G	100.0%	351.9 / NA	87	D621A (GAT→GCT)	ptsP	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (52/35);  total (52/35)

TTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTT  >  NC_000913/2966425‑2966717                                                                                                                                                     |                                                                                                                                                 ttCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGAATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCg                                                                                                                                                >  2:1216763/1‑151 (MQ=255)  tCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGt                                                                                                                                               >  2:333494/1‑151 (MQ=255)    aCCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTg                                                                                                                                             <  2:287554/151‑1 (MQ=255)       gCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTg                                                                                                                                          <  1:753417/151‑1 (MQ=255)       gCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTg                                                                                                                                          >  2:823399/1‑151 (MQ=255)         acaAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGc                                                                                                                                         >  1:24626/1‑150 (MQ=255)               ggAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAAc                                                                                                                                     >  2:1214536/1‑148 (MQ=255)                   aTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATg                                                                                                                              >  1:27923/1‑151 (MQ=255)                     cGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGa                                                                                                                             >  1:1082571/1‑150 (MQ=255)                     cGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGa                                                                                                                             >  1:258188/1‑150 (MQ=255)                     cGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGa                                                                                                                             >  2:64448/1‑150 (MQ=255)                          ccATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCg                                                                                                                        >  2:1165406/1‑150 (MQ=255)                            aTGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGAcc                                                                                                                     <  1:1181969/151‑1 (MQ=255)                                 tttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCtt                                                                                                                >  1:467416/1‑151 (MQ=255)                                    ccGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGc                                                                                                             <  1:617074/151‑1 (MQ=255)                                      gCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCa                                                                                                           <  1:315936/151‑1 (MQ=255)                                        ttCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                            >  2:343950/1‑148 (MQ=255)                                        ttCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                            >  1:1202492/1‑148 (MQ=255)                                        ttCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                            >  2:968144/1‑148 (MQ=255)                                         tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                            >  1:1271964/1‑147 (MQ=255)                                         tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGc                                                                                                             >  1:96767/1‑146 (MQ=255)                                         tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAg                                                                                                          <  1:989805/149‑1 (MQ=255)                                         tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAg                                                                                                          <  1:1082714/149‑1 (MQ=255)                                         tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAg                                                                                                          <  2:867924/149‑1 (MQ=255)                                          cccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATg                                                                                                       >  1:1041557/1‑151 (MQ=255)                                          cccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATg                                                                                                       <  2:1040819/151‑1 (MQ=255)                                           ccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGc                                                                                                      >  1:288582/1‑151 (MQ=255)                                             gggCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCgg                                                                                                    <  1:1242506/151‑1 (MQ=255)                                              ggCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgc                                                                                                   <  2:765189/151‑1 (MQ=255)                                               gCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgc                                                                                                   >  2:958338/1‑150 (MQ=255)                                                      tcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATaa                                                                                            >  1:1209680/1‑150 (MQ=255)                                                       cGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTCGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAAt                                                                                          <  1:149927/151‑1 (MQ=255)                                                           agagCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATAcc                                                                                       >  2:743900/1‑150 (MQ=255)                                                            gagCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCAt                                                                                     >  1:926078/1‑151 (MQ=255)                                                               cTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTg                                                                                   >  1:373359/1‑150 (MQ=255)                                                                 cGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGCGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGa                                                                                  >  1:1098687/1‑149 (MQ=255)                                                                 cGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGa                                                                                  <  2:241773/149‑1 (MQ=255)                                                                  gTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACgg                                                                               >  1:1232848/1‑151 (MQ=255)                                                                  gTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACgg                                                                               >  1:1293533/1‑151 (MQ=255)                                                                  gTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACgg                                                                               >  2:1022061/1‑151 (MQ=255)                                                                       aTTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACtt                                                                          >  1:877959/1‑151 (MQ=255)                                                                         tGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTcc                                                                        >  1:1257643/1‑151 (MQ=255)                                                                          gCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTcc                                                                        <  1:712859/150‑1 (MQ=255)                                                                          gCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTc                                                                         <  2:444622/149‑1 (MQ=255)                                                                           cAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTcc                                                                        >  2:1265607/1‑149 (MQ=255)                                                                           cAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTcc                                                                        >  1:29605/1‑149 (MQ=255)                                                                            aGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAg                                                                      >  2:822246/1‑150 (MQ=255)                                                                            aGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGc                                                                     >  1:1256225/1‑151 (MQ=255)                                                                             ggATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCa                                                                    <  2:1281012/151‑1 (MQ=255)                                                                                 gAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatgat                                                                <  2:769595/151‑1 (MQ=255)                                                                                  aaGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatgatg                                                               >  1:623238/1‑151 (MQ=255)                                                                                    gACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGcc                                                             <  2:579436/151‑1 (MQ=255)                                                                                    gACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGcc                                                             <  2:206781/151‑1 (MQ=255)                                                                                     aCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCg                                                            <  1:510758/151‑1 (MQ=255)                                                                                     aCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCg                                                            >  2:876061/1‑151 (MQ=255)                                                                                     aCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCg                                                            <  2:219239/151‑1 (MQ=255)                                                                                       tGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                          >  1:781437/1‑151 (MQ=255)                                                                                        gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                          <  1:149022/150‑1 (MQ=255)                                                                                        gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                          <  2:45312/150‑1 (MQ=255)                                                                                          cATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATAc                                                        <  2:180448/150‑1 (MQ=255)                                                                                          cATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATAc                                                        <  1:264555/150‑1 (MQ=255)                                                                                           aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACg                                                       >  1:902302/1‑150 (MQ=255)                                                                                             aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTgg                                                    <  1:273310/151‑1 (MQ=255)                                                                                             aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTg                                                     <  2:110439/150‑1 (MQ=255)                                                                                             aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGAAGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGCGGCCGCTACGTgg                                                    >  1:29114/1‑151 (MQ=255)                                                                                                     gCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGaa                                            <  2:892684/151‑1 (MQ=255)                                                                                                      ccACCCGGGTATTGTT‑CGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAtt                                          <  1:440476/151‑1 (MQ=255)                                                                                                        aCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAttt                                         >  1:796151/1‑151 (MQ=255)                                                                                                        aCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAttt                                         >  1:138652/1‑151 (MQ=255)                                                                                                           cGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGt                                      <  2:975063/151‑1 (MQ=255)                                                                                                             ggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTTCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTa                                     <  2:748255/150‑1 (MQ=255)                                                                                                                     tgCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatca                             <  2:444968/150‑1 (MQ=255)                                                                                                                       cGATCAACGGCCAGAGTGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGGTTGGGAATTTAGTAACCGatcat                            >  1:1284360/1‑149 (MQ=255)                                                                                                                       cGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCAtct                          >  1:280571/1‑151 (MQ=255)                                                                                                                                 ccAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTc                <  1:1283587/151‑1 (MQ=255)                                                                                                                                  cAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTc                >  1:930072/1‑150 (MQ=255)                                                                                                                                  cAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATG‑AATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCAc              >  1:483833/1‑151 (MQ=255)                                                                                                                                   aGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCAc              >  1:899448/1‑151 (MQ=255)                                                                                                                                     aaTGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt            <  2:186437/151‑1 (MQ=255)                                                                                                                                     aaTGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt            >  2:975114/1‑151 (MQ=255)                                                                                                                                        gTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGCCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCg         >  2:207735/1‑151 (MQ=255)                                                                                                                                          aTTGAGTCAGAGCGTTGGTGCCAACAAAGATGGAATCGACCCGCTGTGCCGGATGCGGCAGCATAAAGAACATTGACGGCATTTCCAGCATGATGCCGCTACGTGGTTTGGGAATTTCGTAACCGATCATCGCCTCGACTTCACGTCCg         <  1:241726/149‑1 (MQ=255)                                                                                                                                            tGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAc     >  2:705375/1‑151 (MQ=255)                                                                                                                                            tGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAc     >  2:1205418/1‑151 (MQ=255)                                                                                                                                            tGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAc     >  1:872514/1‑151 (MQ=255)                                                                                                                                              aGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGt   >  1:1297446/1‑151 (MQ=255)                                                                                                                                               gTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGtt  >  2:59460/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTT  >  NC_000913/2966425‑2966717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑