Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,080,8370GT43.7% 62.7 / 37.2 71intergenic (+24/‑519)putP/efeUproline:sodium symporter/ferrous iron permease (pseudogene)
Reads supporting (aligned to +/- strand):  ref base G (12/28);  new base T (31/0);  total (43/28)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACAC  >  NC_000913/1080691‑1080987
                                                                                                                                                  |                                                                                                                                                      
aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTtttgtttg                                                                                                                                                    <  1:625418/151‑1 (MQ=255)
    ttGGGATTGTAGTGTTTAGTTTGCTTGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCtt                                                                                                                                                >  1:102093/1‑151 (MQ=255)
    ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTgg                                                                                                                                                   >  1:899252/1‑148 (MQ=255)
    ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTGTTTGGCtt                                                                                                                                                >  2:1301574/1‑151 (MQ=255)
      gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTc                                                                                                                                               <  2:443239/150‑1 (MQ=255)
      gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTc                                                                                                                                               <  1:609530/150‑1 (MQ=255)
       ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGtt                                                                                                                                                >  2:1001809/1‑148 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTcagca                                                                                                                                           >  2:533473/1‑149 (MQ=255)
            gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCagcagc                                                                                                                                         <  1:891452/150‑1 (MQ=255)
              aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCCGCAGCggg                                                                                                                                      >  2:275124/1‑151 (MQ=255)
               gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCggg                                                                                                                                      >  2:150047/1‑150 (MQ=255)
                 ggttAGTTTGCGGGGTAAAGCGCCGTCAGCCGCGATGCAAAAACGCTTTGCCGACGCCGATGCGCACTAACATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTg                                                                                                                                   <  1:1052245/149‑1 (MQ=255)
                  tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCCTCCCCAACggggt                                                                                                                                    >  1:971944/1‑147 (MQ=255)
                      gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTGTTTTGCTTTCGCCGCggggt                                                                                                                                    >  1:213676/1‑143 (MQ=255)
                           cTGGGTAAAGCGCCGTCAGGGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTCGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCtt                                                                                                                          <  1:1015964/150‑1 (MQ=255)
                               gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAtgt                                                                                                                     <  1:1071012/151‑1 (MQ=255)
                                 aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCAGCAGCGGGGTGCGCTCCCtt                                                                                                                          >  1:763489/1‑144 (MQ=255)
                                 aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAtgtg                                                                                                                    <  1:957663/150‑1 (MQ=255)
                                 aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAtgtg                                                                                                                    <  2:197874/150‑1 (MQ=255)
                                   aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCCTCAACAGCGGGGTGCGCTCCCCTAATGTGcc                                                                                                                  >  2:1205112/1‑150 (MQ=255)
                                   aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCt                                                                                                                 <  2:521281/151‑1 (MQ=255)
                                    gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGTTTCAGCAGCGGGGTGCGCCCCCTTTAtgt                                                                                                                     >  1:85986/1‑146 (MQ=255)
                                      gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGc                                                                                                              <  2:1290143/151‑1 (MQ=255)
                                        cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGTTTTTGCAGCCGGGGGCGCTCCCCTTAtgtggccccccc                                                                                                            >  2:763103/1‑143 (MQ=255)
                                            aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCCTTCGCAACGGGGTTCGGCCCCTTAATTGGCCTccccc                                                                                                            >  1:16378/1‑146 (MQ=255)
                                              cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATaa                                                                                                       >  2:1225936/1‑150 (MQ=255)
                                                  gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTTAGCAGCGGGGTGCGCTCCCCTTATTTGCCTccccctcttaa                                                                                                      >  2:1065422/1‑140 (MQ=255)
                                                   aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCCGCCACGGGGTGCGCCCCCTTAATGTGCCTCCCCCTATaaaat                                                                                                    >  1:133416/1‑146 (MQ=255)
                                                      cAAAAACGCTTTGCCGCGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGACTTGTTTGGCGTTAGCACCCGGTTGCTGTCCCTTTTTGTGCCTCGCCATATAAAtattattg                                                                                              >  2:865518/1‑144 (MQ=255)
                                                       aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCCGCAGCGGGGTGGGCTCCCCTTATTTGGCTCGCCATATAAAATTAATgg                                                                                              >  1:1227806/1‑150 (MQ=255)
                                                       aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCCTCAGCAGCGGGGTGCGCCCCCTTAATGTGCCCCCCCCTATAAATTGAATgg                                                                                              >  1:328059/1‑150 (MQ=255)
                                                        aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTg                                                                                            <  1:1050554/151‑1 (MQ=255)
                                                           aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAGAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTTTGCAGCGGGGTTCGGTCCCCTAATTTGGCTCGCCCTATAAATTGaa                                                                                                 >  1:567523/1‑143 (MQ=255)
                                                           aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCTGCAGCGGGGTGTGCTCCCTTAATGTGCCTCGCCCTATAAATTTGATgggggcg                                                                                         >  2:1200701/1‑146 (MQ=255)
                                                           aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAg                                                                                         <  2:419534/151‑1 (MQ=255)
                                                               tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGATTTGTTTTGCTTCAACCACGGGTTGCGCTCCCCTTATGTGACCCGCCCTATAAAATTAATAGGGCAagg                                                                                       >  2:186195/1‑149 (MQ=255)
                                                                     gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTTCGCTCCCCTTATGTGCCTCCCCCTATAAATTGAATGGGGCCGGGAgcg                                                                                   >  2:384111/1‑147 (MQ=255)
                                                                     gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGGTTCAGCAGCGGGGTGCGCTCCCTTAATTTGCCTCCCCCTATAAATTTAATGGTGCAGGGAgcgc                                                                                  >  2:457401/1‑148 (MQ=255)
                                                                     gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGCTTCCGCAGCGGGGTGGGCTCCCCTTATGTGGCTCCCCCTATAAATTTGATGGGGGAGGGGgcgcgc                                                                                >  1:1148918/1‑150 (MQ=255)
                                                                        gCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAggg                                                                            <  2:280259/151‑1 (MQ=255)
                                                                           gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGGTTCAGCAGCGGGGTGCGCCCCCTTTATGTGCCTCGCCCTATAAATTTAATGGGGGAGGGGGGGCGCAgggggg                                                                         >  1:673258/1‑150 (MQ=255)
                                                                           gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggc                                                                         <  2:1091566/151‑1 (MQ=255)
                                                                            aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCCTCACGAGCGGGGTTGGCTCCCCTAATGTGCCTCGCCCTATAAATTTAATGGTGCAGGGAGCGCGGCgggggg                                                                         >  1:217930/1‑149 (MQ=255)
                                                                              gcgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggcggc                                                                      <  1:543916/151‑1 (MQ=255)
                                                                               cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGcc                                                                     <  2:645324/151‑1 (MQ=255)
                                                                                  aCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCATCAGCGGGTTTCTGACCCCTAATGTGTCTCTCCACATAAAATGAATGGGGGGGGGAGCGGCCAGGGGGGGGccca                                                                   >  2:1025452/1‑148 (MQ=255)
                                                                                  aCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAACGGGGTGCGCTCCCTTAATGTGGCTCGCCATATAAATTTAATGGTGCAGGGAGCGCGCAGGGGGGGGCCaa                                                                   >  2:1287104/1‑150 (MQ=255)
                                                                                   cTAGCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAt                                                                  <  1:1229867/150‑1 (MQ=255)
                                                                                    tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGTTTCAGCAGGGGGTTTCGGTCCCCTAATTTGGCTTGCCATTTAAAAAGAATGGGGTAGGGGGCGGGCCGGGGGGGGCCa                                                                    >  2:270530/1‑147 (MQ=255)
                                                                                    tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGGTTCCGCAACGGGGTGGGCCCCCTTAAAATGGCCCCCCCTCTAAATTGAATGGTGCAGGGGGCCGGCAGGGGGGGGGCCATc                                                                 >  1:1234770/1‑150 (MQ=255)
                                                                                          tCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGCTCCGCAGCGGGGTTCGCCCCCCTTATGTGGCTCCCCCTTTAAATTTAATGGGGCAGGGGGGGCGCCGGGGGCGGCCAAAcgccccc                                                           >  1:984733/1‑150 (MQ=255)
                                                                                                ccgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCct                                                    <  1:255153/151‑1 (MQ=255)
                                                                                                 cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAACAGCCGGGTGCGGCCCCCTTATGTGGCTCGCCCTATAAAATGAATTGGGCAGGGGGCGCGCCGGGGGGGGGCCAACGCCGcccccccc                                                     >  1:1098823/1‑149 (MQ=255)
                                                                                                   ccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTCAGCAGCGGGGTGCGCTCCCCTTATGTGCCTCGCCATATAAATTGTAAAGGGCCGGGGGGGCGCCGGGGGGGGCCCAACGCccccccccccc                                                    >  1:591482/1‑147 (MQ=255)
                                                                                                   ccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCctgct                                                 <  2:298207/151‑1 (MQ=255)
                                                                                                       cACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCCCCCCTAATGTGCCTCGCCCTATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCCCCCCCGCCCCCCGCTGTcc                                             >  2:337630/1‑151 (MQ=255)
                                                                                                          ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTTAGCAGCGGGTTGCGCTCCCCTAATGTGCCTCGCCCTATAAAATTAATTGGGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCCCCCCCTGCTGTccc                                            >  1:56127/1‑149 (MQ=37)
                                                                                                          ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCgg                                          <  2:673281/151‑1 (MQ=255)
                                                                                                          ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGGTTCAGCAGCGGGGTGCGCTCCCCTTATGTGGCTCCCCCTATAAATTTAATGGTGGAGGGGGGCCGCCGGGGGGGGCCAATCCCCGccccccccccccgttcccg                                          >  1:135243/1‑140 (MQ=255)
                                                                                                           gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTTATGTGCCTCGCCATATAAATTTAATTGTGGAGGGAGCGCGCAGGGGGCGGGCCATCGCCGCCCCCCCCCGCTGTccccg                                          >  2:991434/1‑148 (MQ=255)
                                                                                                             tGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGGTTCAGCAGCGGGGTGCGCTCCCCTAATGTGCCTCGCCATATAAAATGAATTGGGCCGGGGGCGCGCCGGGGGGGGCCAATCGCCGCCCCCCCCCGCTGTCCCGGcc                                        >  2:481215/1‑150 (MQ=255)
                                                                                                              gCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                      <  1:149612/151‑1 (MQ=255)
                                                                                                                 ggAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCgg                                   <  2:861519/151‑1 (MQ=255)
                                                                                                                   aaaGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTTATGTGCCTCGCCCTATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCCTCgggg                                 >  2:694828/1‑151 (MQ=255)
                                                                                                                        tAAGGGACTTAGCCTGCGGCGGGTTTTTTTGGGTTCCGCAGCGGGGTGGGCTCCCCTAATTTGGCTCGCCCTATTAATTTAATGGTGGCGGGAGCGCGCCGGGGGGGGCCAAACCCCGCCGCCCCCCGCTGTCCCCGCCCTCGGGGGACg                             >  1:600352/1‑150 (MQ=255)
                                                                                                                           gggACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCtt                          <  1:1017037/150‑1 (MQ=255)
                                                                                                                             gACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAg                       <  1:1029977/151‑1 (MQ=255)
                                                                                                                                 tAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCg                     <  2:247876/149‑1 (MQ=255)
                                                                                                                                       gcggcgGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTg               <  2:1227881/149‑1 (MQ=255)
                                                                                                                                       gcggcgGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTg               <  2:961255/149‑1 (MQ=255)
                                                                                                                                         ggcggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc           <  2:1049425/151‑1 (MQ=255)
                                                                                                                                            ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac        >  2:792648/1‑151 (MQ=255)
                                                                                                                                                  gtttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAacac  >  1:60892/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
AGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACAC  >  NC_000913/1080691‑1080987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: