Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	43.5%	107.2 / 22.5	69	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (11/28);  new base G (30/0);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.79e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATA  >  NC_000913/2434739‑2435031                                                                                                                                                 |                                                                                                                                                       cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCAc                                                                                                                                                 <  2:491042/151‑1 (MQ=255)    atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCCGGCAGGGGAATCCAcc                                                                                                                                                >  2:365445/1‑149 (MQ=255)    atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcg                                                                                                                                               <  1:1541/150‑1 (MQ=255)      ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgcg                                                                                                                                             <  2:1154226/150‑1 (MQ=255)      ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgcg                                                                                                                                             <  1:396903/150‑1 (MQ=255)       tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATTATAACGGTCCGGGGAAttcccc                                                                                                                                                >  2:397695/1‑141 (MQ=255)        gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc                                                                                                                                           <  1:1291379/150‑1 (MQ=255)           tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGGTTTGAAGATGAAACCGGCGGGGGTGATCCAcc                                                                                                                                                >  2:557492/1‑142 (MQ=255)               ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGGCCGGGGAATCCACCGCGACCAGAT‑a                                                                                                                                    >  2:36013/1‑149 (MQ=255)                cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACGGTGAGGGGAATACCCCCCGACCAGAT‑a                                                                                                                                    >  1:1019469/1‑148 (MQ=255)                cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGGTCATAACGGGCAGGGGAATCCACCGCGGACAGaaa                                                                                                                                     >  1:837235/1‑146 (MQ=255)                    tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGATTTTGGAGATCATACCCGACAAGGGAATTCACCCCCACCAGGT‑ACa                                                                                                                                  >  2:965589/1‑146 (MQ=255)                     aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGGAGATCATAACGGTCAGGGGAAACCACCGCGACCACAT‑AAAGcccct                                                                                                                            >  1:1201084/1‑149 (MQ=255)                     aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATAAAAGCGGGCAGGGGAATCCACCGCGACCGGAT‑ACAGccc                                                                                                                              >  2:1059314/1‑149 (MQ=255)                         gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACCGTCCGGGGAAATCACCGCCACCCGAT‑ACAGCCCCTcc                                                                                                                          >  2:112891/1‑149 (MQ=255)                           cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCATAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGGCCCGAT‑ACAGCCCCTCCGCtt                                                                                                                      >  2:497387/1‑151 (MQ=255)                            aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGGCAGGGGAAACCACCCCGACCAGAA‑ACAGCCCCTCCCCttt                                                                                                                     >  2:644600/1‑151 (MQ=255)                               tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATTATACCCGTCAGGGGAATCCACCCCGACCAGAT‑ACAGCCCTTCCCCtttt                                                                                                                    >  1:1141600/1‑149 (MQ=255)                                ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCtttt                                                                                                                    <  1:650471/148‑1 (MQ=255)                                ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTg                                                                                                                   <  1:510157/149‑1 (MQ=255)                                       tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGGTCATTCCGGTCAAGGGAATACACCGCGCCCAGCT‑ACAACCCCTCCGCTTTTGTCg                                                                                                                >  2:1035604/1‑145 (MQ=255)                                        gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTtgctgc                                                                                                         <  2:646770/151‑1 (MQ=255)                                         tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATACCGGGCAGGGGAAACCACCCCGACCCGAT‑ACAGCCCCTCCCCTTTTTCCGTTGCTgcc                                                                                                        >  1:539441/1‑151 (MQ=255)                                              aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCa                                                                                                    <  1:328499/150‑1 (MQ=255)                                                 ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACGGTGAGGGGAATACACCCCGCCCAGAT‑ACAGACCCTCCGCTTTTGCCGTTGCTGCCCCCAGCGt                                                                                                >  2:297981/1‑151 (MQ=255)                                                  tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTTGAAGAACATTCCGGTTAGGGGAATCCAACCCGACCCGAA‑ACAGCCCTTCCTCTTTTTTCGGTGCTgccccc                                                                                                     >  2:858962/1‑145 (MQ=255)                                                      tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGGAGATCATACCCGGCAGGGGAATCCACCGCGACCAGAT‑AAAACCCCTCCCCTTTTTCCCTTGCTGCCGCCAGCggt                                                                                               >  2:1083181/1‑145 (MQ=255)                                                       aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGt                                                                                           <  1:1154789/150‑1 (MQ=255)                                                         aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCCTACCGGTCGGGGGAATCCACCGCGGCCAAAA‑ACAGACCTTCCGCTTTTGCCGTTGCTGCCGCCCACGCTCTGTcc                                                                                         >  2:1201408/1‑150 (MQ=255)                                                          gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                                                       <  1:1114612/151‑1 (MQ=255)                                                             tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGGCCAGAT‑AAAGCCCTTCCGCTTTTTCCGGTGCTGCCGCCCCCGTTCTGGCCttt                                                                                      >  2:435220/1‑149 (MQ=255)                                                               aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGTAGATCATACCGGGCCGGGGAATCCACCCCGACCAGAT‑ACAGCCCCTCCCCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTTCttt                                                                                      >  1:676668/1‑147 (MQ=255)                                                                aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTTGGAGATTCTACCCGGCAGGGTAATCCACCCCGGCCAACT‑AGAGACCCTCCCCTTTTGTCGTTCGTGGCGCCCGCCGTGTGTCCTTTgcccc                                                                                 >  1:112888/1‑149 (MQ=255)                                                                 aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGAACATAACGGGCAGGGGAATCCACCCCGGCCAGAT‑AAAGCCCCTCCCCCTTTGCCGTTGCTGCCGCCCGCGGTCTGTCCTTTtccccc                                                                                >  2:556507/1‑151 (MQ=255)                                                                       cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGAGCATACCGGTCCGGGGAATCCACCCCGGCCAGAT‑ACAACCCTTCCCCTTTTGTCCTTTCTGCCCCCCGCGGTCTTTTCTTTtccgcccccca                                                                           >  2:397203/1‑145 (MQ=255)                                                                          gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                                                       <  2:174819/151‑1 (MQ=255)                                                                             ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAAATTATACCGGTTAGGGGAATTCACCCCGACCAGAT‑AAAGCCCTTTCGCTTTTTTCGTTTCCGCCGCCAGCGGTCTGTCCTTTTCCGCCCGCaa                                                                           >  2:1268885/1‑144 (MQ=255)                                                                               attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTAt                                                                  <  2:1301005/151‑1 (MQ=255)                                                                                ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTGGTAGATCAAACCGGTCCGGGTGATCCACCGCCACCCGATAACCCCCCTCCCCCTTTTGCCCTT‑CTGCCGCCAGCGTTTTTTCCCTTTCCGCCCGCAActcttc                                                                     >  2:402420/1‑145 (MQ=255)                                                                                    cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTGGGAGATCATACCCGGCAGGGGAATCCACCCCGCCCAGAT‑ACAGCCCCTTCCCTTTTTCCGTTGCTGCCCCCCGCGCTCTGTCCTTTTCCCCCAGCCAcccctctttccc                                                               >  1:118173/1‑138 (MQ=255)                                                                                      ttAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATTAAACCGGGCCGGGGAAACCCCCCCGACCCGAA‑AAAACCCCTCCCCTTTTTCCGTTTGTGCCCCCCACGGTCTGTTCTTTTGCGCCCCCCACCCTGCTTTCCAGc                                                             >  2:779382/1‑149 (MQ=255)                                                                                       tAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGTAGAACATACCGGTCAGGGGAAAACACCCCGACCAGAG‑ACAGCCCTTTCGCCTTTTCCGGTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCt                                                                    >  1:500129/1‑141 (MQ=255)                                                                                           ccGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATAACCGTCCGGGGAATCCACCCCGACCAGAT‑ACAGCCCCTTCGCCTTTGCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCCGctct                                                          >  2:1242882/1‑147 (MQ=255)                                                                                           ccGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                       <  2:137710/150‑1 (MQ=255)                                                                                              ccAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATAATAACGGGCAGGGGAATACACCGCGACCAAAT‑ATACCCCTTTCCCTTTTGGCGCCGCTGTCCCCAACGTTCTGTCCTTTTGCCCCCGCAACACTGCTATCCAGCTCTCCGGCTg                                                   >  2:739185/1‑151 (MQ=255)                                                                                                aGAAATAGCGGGCCCATTGGCGGGTTTTGGAAAAT‑ATAACCGGCAAGGGAAACCACCGCGACCAGAA‑ACAACCCCTCCCCCTTTTCCGGTGGTGGCCCCAGGGGTTTGTCCCTTTCCCCCCGCAACCCCGCTATCCAGCTcccccggcgg                                                  >  1:1034356/1‑144 (MQ=255)                                                                                                 gAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                                <  1:613107/151‑1 (MQ=255)                                                                                                  aaaTAGCGGGCCCATTGGC‑GGTTTTGGAAGAACATACCCGGCAGGGGAAACCACCCCGACCAGAG‑ACAGCCCTTCCGCTTTTTCCGGTGCTGCCCCCCGCGGTCTTTCCTTTTTCGCCCGCCACTCCGCTCTTCCGCTCTCCGGcctggt                                                >  1:463478/1‑145 (MQ=255)                                                                                                      aGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                            <  1:407075/150‑1 (MQ=255)                                                                                                             ccATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc                                    <  2:1270521/151‑1 (MQ=255)                                                                                                               aTTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACtt                                  <  1:526891/151‑1 (MQ=255)                                                                                                                 tGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                                >  1:410059/1‑151 (MQ=255)                                                                                                                  ggC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                                >  2:79240/1‑150 (MQ=255)                                                                                                                         tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                          <  1:616410/150‑1 (MQ=255)                                                                                                                         tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                         >  1:1050674/1‑151 (MQ=255)                                                                                                                           tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                        <  2:800588/150‑1 (MQ=255)                                                                                                                           tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                       >  2:547443/1‑151 (MQ=255)                                                                                                                            ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                      >  1:199107/1‑151 (MQ=255)                                                                                                                             gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                     <  1:550385/151‑1 (MQ=255)                                                                                                                              aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                    >  1:1091015/1‑151 (MQ=255)                                                                                                                                 aTCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGAc                  >  2:328833/1‑150 (MQ=255)                                                                                                                                 aTCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                 <  1:907885/151‑1 (MQ=255)                                                                                                                                       ccGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc           >  2:374892/1‑151 (MQ=255)                                                                                                                                         ggTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt         <  2:183040/151‑1 (MQ=255)                                                                                                                                          gTCAGGGTAATCCACCGCGACCAGAT‑ACAGTCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg        <  2:716584/151‑1 (MQ=255)                                                                                                                                            cAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa       <  2:463490/150‑1 (MQ=255)                                                                                                                                            cAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa       <  2:332451/150‑1 (MQ=255)                                                                                                                                            cAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc      <  1:2724/151‑1 (MQ=255)                                                                                                                                                gTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTTCCGACAATATTCCTGACCata  <  2:1200713/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                       CATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAT‑ACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATA  >  NC_000913/2434739‑2435031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑