Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	41.7%	105.0 / 37.2	72	L24F (TTA→TTT)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (34/8);  new base A (0/30);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 3.62e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417                                                                                                                                                         |                                                                                                                                                      gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCTTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataaa‑t                                                                                                                                                       >  1:1203511/1‑151 (MQ=255)  ttCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataaa‑t                                                                                                                                                       >  1:1227648/1‑150 (MQ=255)   tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataaa‑ta                                                                                                                                                      <  2:310941/150‑1 (MQ=255)    cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑Aataaa‑taa                                                                                                                                                     <  2:353592/150‑1 (MQ=255)     gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑Taaaa                                                                                                                                                   <  1:718095/151‑1 (MQ=255)     gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑Taaaa                                                                                                                                                   <  1:325730/151‑1 (MQ=255)      tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AAtaaa‑taaa                                                                                                                                                    >  1:1166040/1‑149 (MQ=255)        tatttATGATTAACGACAGAGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCATGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACt                                                                                                                                                 >  1:713421/3‑150 (MQ=255)        tCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑Taaaa                                                                                                                                                   >  1:431597/1‑148 (MQ=255)          tttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTAcc                                                                                                                                              >  1:315604/1‑151 (MQ=255)                aTTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCaa                                                                                                                                         <  1:701331/150‑1 (MQ=255)                   aaCGACAGTGACACTACCTGAGACTCGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATaa                                                                                                                                      >  1:933709/1‑150 (MQ=255)                    aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATaa                                                                                                                                      >  2:572315/1‑149 (MQ=255)                       aCAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGaa                                                                                                                                  >  2:326186/1‑150 (MQ=255)                          gTGACACTCCCTGAGTTTTGTGAAAAAATTGGATCTGCTCCCCCCGTAAAATAGGCGTCATTTGTACAAATAATGTCTTCAAATCCCATTGACAAAAAGAAAAAGCCACCAAAAAACGA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGt                                                                                                                              <  1:1009460/151‑1 (MQ=255)                                 tACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTttct                                                                                                                       >  2:569005/1‑151 (MQ=255)                                  ccctgacccttggcgaGAAATTGAACTGGCATTCCCCGTGACATAGTCGCCATTTGTACTAACATTGTTTTCTAATTCCATAG‑CCAAAAAGGAAAAGTACCTTAAAACCCAAAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTttct                                                                                                                       <  1:852916/135‑1 (MQ=255)                                   cttGAGATTTGTGAAAAAATTGGATATGCTTCCCCCGTAACATAGGCGTCATTTGTATTAATATTTTTTTTTAATTCCATTGACCAATAGGCAAAGCCCCCTAAAACCGA‑AAAAAA‑AAAACCTACCGCCAATAAAGAAAAGTAttt                                                                                                                          <  2:707602/144‑1 (MQ=255)                                   ccTGAGATTTGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTTTACAAATCATGTCTTCAAATTCCATTAACAAAAAGGCATAGGCCCCTAAAAACGC‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGt                                                                                                                              <  2:1175831/142‑1 (MQ=255)                                   ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTttcttc                                                                                                                     >  2:536064/1‑151 (MQ=255)                                   ccTGAGACTTGTGAAAAAATTGAATCTGCATTCCCCGTAACATAGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGACAAATAGGCATAGGCCCCTGAAACCGC‑AATAAA‑AAAACCTACCGCCAATAAAGAAAAGTATTttctt                                                                                                                      <  1:150381/150‑1 (MQ=255)                                             gtgAAGAAATTGATTCTGCATCCCCCGTGAAATAGGCGTCATCTTTACAAATCATGTCTTCTAATTCCATTGACCAATAGGAAAGGGCACCTGAAAACGA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                            <  2:286052/150‑1 (MQ=255)                                                 aaaatttGGTTCTTCTTCCCCCGGGACATGGGCTTCATTTGTACTAATTAT‑TTTTTTAATTCCCATGAGCAAAAGGGCTGGGGCCCCGAAAAGCC‑AAAAAATTAAATTTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt                                                                                                         <  2:1212505/144‑1 (MQ=255)                                                   aatttGGTTCTACATTCCCCGTAACATAGGGTTCCGTTTTTCTAATCTTGTCTTCTAATTCCATTAACAAACAGGCATGGGCATTTGAAAACAA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa                                                                                                              <  2:292393/139‑1 (MQ=255)                                                   aaaTTGGATCTGCATCCCCCGTAACATAGGCGTCATCTGTACAAATAATGTTTTCAAATTCCATTCCCAAAAAGAAATAGACACCTAAAACCGC‑AAAAAA‑AAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                                      <  1:496753/150‑1 (MQ=255)                                                    aaTTGGTTCTGCATTCCCCGTGACATAGGCGTCATTTGTACTAAACATGTCTTCAAATTCCATTCACAAAAAGAAATAGCCACCTGAAAACAA‑AATAAA‑AAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                    <  1:1203134/151‑1 (MQ=255)                                                      tggtatttacATTCCCCGATACATGGGCCTCATCTTTAAAAAACTTTTCTTCAAATCCAATTGACAAATAGACAGAGACTCCTAAAATCCA‑AATAAA‑AAAAACTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                    <  1:1144487/140‑1 (MQ=255)                                                        ggttCTGCATTCCCCGTGAAATAGGCGTCATCTGTACTAATCATTTCTTCAAATTCCATTGACCAAAAGGAATAGGCCCCTAAAAACGA‑AATAAA‑AAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                 <  1:557894/147‑1 (MQ=255)                                                        ggACCTCCATCCCCCGTGACATAGGCGTAATCTGTATAAATCATGTCTTCAAATCCCATTACCAAATAGGAATAGCCCCCTAAAAACAA‑AAAAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                 <  2:164181/150‑1 (MQ=255)                                                          aTCTCCATTCCCCGGGAAATAGGCGTCATCTTTACAAATCTTTTCTTCAAATCCCATTGACCAATAGGCAAAGCCCCCTGAAAACGA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                 <  1:1289640/148‑1 (MQ=255)                                                           tCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                                             >  1:131850/1‑151 (MQ=255)                                                            cTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt                                                                                            >  2:917715/1‑151 (MQ=255)                                                              gCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                           >  2:1279917/1‑150 (MQ=255)                                                               ctttCCCCGTTACAGGGGCGTCATCTGTTCTAATCATGTTTTGAAATGCCATTCACAAATAGACATACCCTCGGAAAAACAA‑AATAAA‑AAAATCTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                           <  2:637743/147‑1 (MQ=255)                                                                aTTCCCCGTGCCATAGGCGCTCTCTGTATTATTCATTTTTTCTAATTAATTTAACAAAGAGGAATAGCCACAGTAAAAGGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                                        <  1:1160955/151‑1 (MQ=255)                                                                  tCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                      >  1:105773/1‑151 (MQ=255)                                                                   ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTAACAAATAGGCATAGGCCCCTAAAAACAA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       <  2:841038/149‑1 (MQ=255)                                                                   ccccGTGAAATAGCCTTCATTTGTCCTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTAAAACCGC‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                      <  2:1020699/150‑1 (MQ=255)                                                                   ccccGTAACATAGGCGTCATCTGTACTAATCATGTTTTCAAATCCCATTGCCAAAAAGGAATAGGCACCTAAAAACAA‑AAAAAA‑AAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       <  1:107200/149‑1 (MQ=255)                                                                    cccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                                    >  2:1271793/1‑151 (MQ=255)                                                                          aCATAGGCGTCATCTGTACTAATCATTTCTTCTAATTCCATTAACCAATAGGCATAGACACCTAAAAACGC‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta                                                                              <  2:869880/151‑1 (MQ=255)                                                                            aTAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt                                                                            >  1:892041/1‑151 (MQ=255)                                                                              aGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                          >  2:214445/1‑151 (MQ=255)                                                                               ggCGTCATTTTTACTAATCATGTCTTCTAATTCCATCGACCAATAGGCATAGGCACGTGAAACCGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                         <  2:760973/151‑1 (MQ=255)                                                                                     aTCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAt                                                                   >  2:899796/1‑151 (MQ=255)                                                                                       cTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt                                                                 >  1:112002/1‑151 (MQ=255)                                                                                         gTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                               >  1:1031870/1‑151 (MQ=255)                                                                                              aataatGTCTTCAATTACCATTGACCAATAGGAATAGCCCCTAAAAAACGA‑AAAAAA‑TAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc                                                              <  1:418179/147‑1 (MQ=255)                                                                                              aaTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                            >  2:620410/1‑149 (MQ=255)                                                                                              aaTCATGTCTTCTAATCCCATTGCCAAATAGACATAGCCCCCTAAAACCGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                                <  1:624073/145‑1 (MQ=255)                                                                                                tCATGTCTTCTAATTCCATTGACCAATAGGCATAGCCCCCTAAAAACGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa                                                         <  2:171991/150‑1 (MQ=255)                                                                                                 cATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAACCGA‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                       <  2:749436/151‑1 (MQ=255)                                                                                                   tttcttctAATTCCATTGACCAATAGGCATAGCCACCTAAAACCGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                        <  2:983794/146‑1 (MQ=255)                                                                                                   tGTCTTCTAATCCCATTGACCAATAGGCAAAGCCACCTGAAAACGC‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa                                                         <  2:521909/147‑1 (MQ=255)                                                                                                    gTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa                                                     >  2:1166647/1‑150 (MQ=255)                                                                                                      cttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                                  >  2:58897/1‑151 (MQ=255)                                                                                                         ctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                               >  2:511953/1‑151 (MQ=255)                                                                                                          aaaTCCCATTAACAAAAAGGAAAAGCCACTTGAAAACCA‑AAAAAA‑AAAATCTCCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa                                                     <  1:603024/143‑1 (MQ=255)                                                                                                           aaTTCCATTGACCAATAGGCATAGGCACCAAAAAACGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                             <  2:1046054/151‑1 (MQ=255)                                                                                                            aTTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                             >  2:56947/1‑150 (MQ=255)                                                                                                                cATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                         >  2:487410/1‑150 (MQ=255)                                                                                                                  tacaCCAATGGCCATAGCCCCCAAAAACCTC‑AAAAAA‑TAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaa                                       <  2:370799/147‑1 (MQ=255)                                                                                                                      ccAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa                                   >  1:695078/1‑150 (MQ=255)                                                                                                                       cAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                  >  2:86473/1‑150 (MQ=255)                                                                                                                        aaTAGGCATAGGCCCCTAAAAACGC‑AATAAA‑AAAAACTCCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag                                <  2:1034249/151‑1 (MQ=255)                                                                                                                          taggcataggcaCCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag                                >  1:863666/1‑149 (MQ=255)                                                                                                                              cataggcaCCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcatt                          >  2:113574/1‑151 (MQ=255)                                                                                                                               ataggcaCCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc                         >  1:351044/1‑151 (MQ=255)                                                                                                                                 aggcaCCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcat                       >  2:607788/1‑151 (MQ=255)                                                                                                                                  gcccccTGAAAACGA‑AATAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat                           <  1:795495/142‑1 (MQ=255)                                                                                                                                       cTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                  >  2:853144/1‑150 (MQ=255)                                                                                                                                       cTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                  >  2:868137/1‑150 (MQ=255)                                                                                                                                         gAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                >  2:331122/1‑150 (MQ=255)                                                                                                                                          aaacCGC‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCa                 <  2:94872/148‑1 (MQ=255)                                                                                                                                          aaaaCGC‑AATAAA‑AAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                <  2:1227723/149‑1 (MQ=255)                                                                                                                                            aaCGA‑AAAAAA‑AAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCa            <  2:352590/151‑1 (MQ=255)                                                                                                                                             aCGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCa            >  2:1056422/1‑150 (MQ=255)                                                                                                                                                   ataaa‑taaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca      <  2:270134/151‑1 (MQ=255)                                                                                                                                                         taaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  2:576944/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                      GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAA‑TAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑