Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC41.2% 98.2 / 18.4 69V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (33/7);  new base C (0/28);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.98e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTT  >  NC_000913/3260906‑3261205
                                                                                                                                                       |                                                                                                                                                      
tGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAc                                                                                                                                                       >  2:631930/1‑151 (MQ=255)
   aGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                    >  2:1249227/1‑151 (MQ=255)
    gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                                                                                   <  1:1195865/151‑1 (MQ=255)
     tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgc                                                                                                                                                  >  1:576883/1‑151 (MQ=255)
      gAATGATATTCAGCGCGCTGATGTACCGCAACGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                                                                                   <  1:504700/149‑1 (MQ=255)
        aTGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGt                                                                                                                                               >  1:1128092/1‑151 (MQ=255)
            tatTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAt                                                                                                                                           >  1:90866/1‑151 (MQ=255)
             atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                          >  2:163710/1‑151 (MQ=255)
                   cgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                      >  2:1068242/1‑149 (MQ=255)
                    gcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                      >  1:987820/1‑148 (MQ=255)
                        tgttatcctcccccGCCAGCAAGTCCATTAAGTAATTGAGGTTGTCCATCACTTTGTTGTAACCCACCACGTGTTCCACCGGCGGTGTTGTTTTCGGCCCGTCTTAAATCTTAATCTTCCCTTCCCCCCCGCCGTTAATTGCGTAGagc                                                                                                                                 <  2:1064468/137‑1 (MQ=255)
                           tttcctcccccGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCCTTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTagag                                                                                                                                  <  2:423613/136‑1 (MQ=255)
                           tGTACCGCCCCGCCAGCCAGTCCATGAAGTGATCGGGGCTGTCCATCATTTTGTCGTAACCCAGCACGTCGTCCACCGGCGGTGTTGTTTTCGGCCCAACCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGagcagc                                                                                                                              <  2:334469/149‑1 (MQ=255)
                            gtccgcccccGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCCTTTTGTCGAAATCCAGCCCGTCTTCCTCCAGCGTTGTTGTTTTCGCCCCGACCGGACTCTTGAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTagag                                                                                                                                  <  1:907394/136‑1 (MQ=255)
                            gTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGtt                                                                                                                           >  1:110470/1‑151 (MQ=255)
                             tACTGCCCCGCCAGCCAGTCCATGAAGTGACCGAGGCTGCCCATCACTTTGTCTTAATCCAGCCCGTCTCCCATCAGCGGTGCTTTTTCGGCCCCGACCGGAATCTCCAGTTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGtt                                                                                                                           <  2:581189/150‑1 (MQ=255)
                                  cccccccacccggTCCATGAAGTGTTCGGGGCTGTCCATCTCTTTGTCGTAAACCACCCCGTGGCCAATCAGCGGTGCTTTTTCCGGCCCGGCCTGACTCTGCGGCTTCTGCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                                                                                      <  2:985643/145‑1 (MQ=255)
                                  ccccGCCAGCCAGCCCATGAAGTGATCGGGGCTGTCCATCTCTTTGTCGTAACCCACCCCGTCCCCCATCAGCGGTGCTTTTTGCGCCCCGACCTAAATCTTCAGCTCCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTc                                                                                                                        <  1:674693/146‑1 (MQ=255)
                                  cACCCCCAGCCGGCCCATGAAGTGATCGAGGCTGTCCATCCCTTTGTCGTAACCCACCCCGTCGCCCATCAGGGGTTCTGTTTCGGCCCCGACCTGAATCTTCCGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGcc                                                                                                                     <  2:414114/151‑1 (MQ=255)
                                           ccagTCCATGAGGTGATCGAGGCTGCCCATCTCTTTGTCGTAACCCAGCACGTAGTCCACCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGTTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAg                                                                                                              <  2:751337/149‑1 (MQ=255)
                                           ccagTCAATGAAGTGATCGAGGCTGTCCTTCACTTTTTCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTGTTTCCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                                                                             <  2:678614/150‑1 (MQ=255)
                                              gTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                                                                                                          >  2:1051377/1‑150 (MQ=255)
                                                ccagaaaGTGATCGGCGCTGTCCATCTCTTTCTCGAAATCCGGCCCGTCCTCCATCAGCGTTGTTGTTTCCGGCCCGCCCTTAATCTTCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTg                                                                                                        <  1:120433/145‑1 (MQ=255)
                                                 cATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGc                                                                                                       >  2:810499/1‑150 (MQ=255)
                                                     aaGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGTCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcc                                                                                                    >  1:21331/1‑149 (MQ=255)
                                                          aTCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGaa                                                                                              >  1:1296796/1‑150 (MQ=255)
                                                          aTCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAAc                                                                                             >  1:689454/1‑151 (MQ=255)
                                                              aGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                                                          >  2:386289/1‑150 (MQ=255)
                                                                 ctctctatcgttttGTGGAAAGCCAGCACTCCCTCCACCAGCGGTGTTTTTTGCGGCCCTACATGAAGCGTCGGTTTTTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa                                                                                         <  2:627024/137‑1 (MQ=255)
                                                                         cACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                                                                               >  2:949368/1‑150 (MQ=255)
                                                                            ttttTCGTATCCCCGCCCGTCGTCCATCAGCGTTGTTGTTTCCGCCCCGCCCTAAATCTTCAGTTTCCCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                  <  1:919961/144‑1 (MQ=255)
                                                                             ttGTCGTAATCCAGCACGTCGTCCATCAGCGGGGCTGTTTTCGGCCCGACCTGAATCATCAGCTTCTCGTCCACCCCGCCGTTAATTGCGCAGAGCAGCGTTATCGCCAGGTTAGCGCGGGCACCAAAGAACTGCATTTGCTTACCAAt                                                                            >  1:131199/1‑149 (MQ=255)
                                                                              tGTCTTAATCCAGCTCGTCTTCAATCAGGGGTGCTTTTTCCGGCCCGACCTAATTCTGCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                           <  2:436389/149‑1 (MQ=255)
                                                                              tGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAc                                                                         >  2:378199/1‑151 (MQ=255)
                                                                              tGTCGTAAGCCACCACGTCGTCCATCCGGGTTTCTTTTTTCGGCCCGACCAAAATCTTCACTTTCGCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaa                                                                             <  2:722932/147‑1 (MQ=255)
                                                                              tGTCGTAACCCAGCCCGTCGCCCATCAGGGGTGTTTTTTTCGCCCCGCCCTGATTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                           <  1:886094/149‑1 (MQ=255)
                                                                               gTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc                                                                        >  1:383723/1‑151 (MQ=255)
                                                                                 cGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACCGCATTTGCTTACCAATCACCAt                                                                      >  2:587995/1‑151 (MQ=255)
                                                                                       ccaccaCTTCTTCCATCAGGGGTGCTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGc                                                                 <  1:1095500/150‑1 (MQ=255)
                                                                                       ccAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCCCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCt                                                                >  1:1079978/1‑151 (MQ=255)
                                                                                        cAGCACGTCGCCCACCGGCGGTGTTGTTTTCGGCCCGCCCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGc                                                                 <  1:1043654/149‑1 (MQ=255)
                                                                                           cACGTCGTCCATCAGGGGTGCTGTTTCCGGCCCGCCCTGATTCTTCAGCTTCCCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGa                                                              <  2:687138/149‑1 (MQ=255)
                                                                                           cACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGGTAGCGCGAGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGa                                                              >  2:682142/1‑149 (MQ=255)
                                                                                                  tcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAgg                                                     >  2:551430/1‑151 (MQ=255)
                                                                                                    cccccggggTGCTTTTTTCCGCCCGGCCCAAATTCTTCGCCTCCGCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                        <  1:208442/140‑1 (MQ=255)
                                                                                                     accggCGGTGTTGTTTTCGGCCCGACCTGAATCTTCTGCTTCCCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCa                                                   <  1:631910/146‑1 (MQ=255)
                                                                                                        aGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcg                                               >  2:337586/1‑151 (MQ=255)
                                                                                                         gCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgc                                              >  1:964460/1‑151 (MQ=255)
                                                                                                           ggTCCTTTTTTCGGCCCGCCTTGATTCTTAAGTTTTGCTTCCTCTCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGt                                            <  2:648471/151‑1 (MQ=255)
                                                                                                            gTGCTGTTTCCGCCCCGGCCTGAATCTTCACCTTCCCCTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgc                                              <  1:260629/148‑1 (MQ=255)
                                                                                                              gCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTaa                                          >  2:1142131/1‑150 (MQ=255)
                                                                                                                  ttttCGCCCCGACCTAAATTTTCAGCTTCCCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAt                                         <  1:1048748/147‑1 (MQ=255)
                                                                                                                  ttttCGCCCCCCCCTCATTCTTCACCTTCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgt                                      <  2:452124/150‑1 (MQ=255)
                                                                                                                  tttcCGGCCCGACCTGATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                        <  1:498453/148‑1 (MQ=255)
                                                                                                                   ttccGGCCCGCCTTAATTCTCCAGCTTCCCCTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                        <  2:497571/144‑1 (MQ=255)
                                                                                                                     tCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                  >  1:898125/1‑151 (MQ=255)
                                                                                                                          cccGACCTAAATCTTCAGCTCCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                  <  1:1274096/147‑1 (MQ=255)
                                                                                                                              aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAg                           >  2:1027090/1‑150 (MQ=255)
                                                                                                                               cgtaaaTTTTCAGCTCCGCGTCCACGCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                         <  1:26244/147‑1 (MQ=255)
                                                                                                                                   aaTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGt                      <  2:521943/150‑1 (MQ=255)
                                                                                                                                      tcgtagccTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCTCCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTAc                    <  1:1009068/143‑1 (MQ=255)
                                                                                                                                              ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               >  2:5717/1‑147 (MQ=255)
                                                                                                                                              ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               >  1:916499/1‑147 (MQ=255)
                                                                                                                                              ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               >  1:1059371/1‑147 (MQ=255)
                                                                                                                                              ttCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAt                <  2:631995/146‑1 (MQ=255)
                                                                                                                                              ttCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg                   <  2:990800/143‑1 (MQ=255)
                                                                                                                                                  cGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCa              <  1:1151758/144‑1 (MQ=255)
                                                                                                                                                   gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc       >  1:462775/1‑150 (MQ=255)
                                                                                                                                                      cACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAttt   >  1:529197/1‑151 (MQ=255)
                                                                                                                                                       aCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtttt  >  2:138420/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
TGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTT  >  NC_000913/3260906‑3261205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: