Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,664	A→C	D41E (GAT→GAG)	yaaI ←	UPF0412 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,664	0	A	C	99.0%	356.0 / ‑4.1	97	D41E (GAT→GAG)	yaaI	UPF0412 family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (46/50);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-01

CTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGG  >  NC_000913/11516‑11810                                                                                                                                                     |                                                                                                                                                   cttTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTc                                                                                                                                                  >  1:857173/1‑151 (MQ=255) cttTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTc                                                                                                                                                  >  2:131041/1‑151 (MQ=255)    ttATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGt                                                                                                                                                <  2:71315/150‑1 (MQ=255)    ttATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGt                                                                                                                                                <  2:253059/150‑1 (MQ=255)     tATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGtt                                                                                                                                               >  2:798315/1‑150 (MQ=255)     tATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTg                                                                                                                                              >  1:512990/1‑151 (MQ=255)         tCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGtt                                                                                                                                           <  1:179768/150‑1 (MQ=255)          cTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTc                                                                                                                                         >  2:983121/1‑151 (MQ=255)           tGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCg                                                                                                                                        >  1:532250/1‑151 (MQ=255)            gAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTc                                                                                                                                         <  1:292989/149‑1 (MQ=255)            gAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTc                                                                                                                                         <  1:370535/149‑1 (MQ=255)                  atatTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAcgcg                                                                                                                                 >  2:135456/1‑151 (MQ=255)                   tatTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCgg                                                                                                                                >  1:426696/1‑151 (MQ=255)                    atTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGc                                                                                                                               >  1:573222/1‑151 (MQ=255)                       cAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCAtt                                                                                                                            >  1:610837/1‑151 (MQ=255)                             ctGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATg                                                                                                                      >  1:1083037/1‑151 (MQ=255)                              tGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATgg                                                                                                                     >  1:405303/1‑151 (MQ=255)                                aCTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGgcg                                                                                                                   >  1:243905/1‑151 (MQ=255)                                   ggCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCg                                                                                                                <  2:125343/151‑1 (MQ=255)                                      gcTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCgagg                                                                                                             >  1:775824/1‑151 (MQ=255)                                         aCGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGt                                                                                                          <  1:683266/151‑1 (MQ=255)                                         aCGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGt                                                                                                          <  1:421136/151‑1 (MQ=255)                                          cGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGtt                                                                                                         >  1:386769/1‑151 (MQ=255)                                           gggCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTGTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGttt                                                                                                        <  1:623589/151‑1 (MQ=255)                                            ggcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTa                                                                                                       <  2:621809/151‑1 (MQ=255)                                            ggcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTa                                                                                                       <  2:595705/151‑1 (MQ=255)                                             gcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTAt                                                                                                      >  1:843505/1‑151 (MQ=255)                                             gcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTAt                                                                                                      >  1:1234123/1‑151 (MQ=255)                                              cggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATg                                                                                                     <  2:1284596/151‑1 (MQ=255)                                                  tttGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTc                                                                                                  >  1:351170/1‑150 (MQ=255)                                                  tttGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCg                                                                                                 >  2:347642/1‑151 (MQ=255)                                                     gAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGCGTTTATGGTCGttt                                                                                              >  1:1121701/1‑151 (MQ=255)                                                     gAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt                                                                                              <  1:878919/151‑1 (MQ=255)                                                      aaaTAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTg                                                                                             >  2:1105486/1‑151 (MQ=255)                                                        aTAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGcc                                                                                           >  2:975751/1‑151 (MQ=255)                                                          aaaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTg                                                                                         <  2:1198530/151‑1 (MQ=255)                                                          aaaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTg                                                                                         <  2:577514/151‑1 (MQ=255)                                                                aTGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGt                                                                                   >  2:1268611/1‑151 (MQ=255)                                                                   cACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgc                                                                                 >  2:1150550/1‑150 (MQ=255)                                                                    aCCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgc                                                                                 >  1:117049/1‑149 (MQ=255)                                                                     ccGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgcagc                                                                              >  1:134098/1‑151 (MQ=255)                                                                      cGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgcagc                                                                              >  2:1248089/1‑150 (MQ=255)                                                                         ttAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcac                                                                            >  2:235025/1‑149 (MQ=255)                                                                           aaCTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcacagca                                                                        <  1:731964/151‑1 (MQ=255)                                                                               gTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATc                                                                      >  1:933375/1‑149 (MQ=255)                                                                               gTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCa                                                                     <  1:1004230/150‑1 (MQ=255)                                                                                 aaaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGc                                                                  >  1:907646/1‑151 (MQ=255)                                                                                   aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTa                                                                <  2:22220/151‑1 (MQ=255)                                                                                      gCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATc                                                             <  2:1002100/151‑1 (MQ=255)                                                                                       ccATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATc                                                             >  1:211808/1‑150 (MQ=255)                                                                                         aTGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGcc                                                          >  1:981630/1‑151 (MQ=255)                                                                                         aTGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGcc                                                          >  2:1001995/1‑151 (MQ=255)                                                                                            gTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAgg                                                       <  2:177134/151‑1 (MQ=255)                                                                                             tCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGc                                                      >  2:952975/1‑151 (MQ=255)                                                                                              cGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCt                                                     <  2:214443/151‑1 (MQ=255)                                                                                               ggCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTg                                                    <  2:236329/151‑1 (MQ=255)                                                                                                gCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTgg                                                   <  1:840063/151‑1 (MQ=255)                                                                                                  agagagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCg                                                 <  1:576587/151‑1 (MQ=255)                                                                                                    agagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGa                                               <  2:374777/151‑1 (MQ=255)                                                                                                      agTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGaa                                              >  1:1169776/1‑150 (MQ=255)                                                                                                          gTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGt                                         >  2:1004526/1‑151 (MQ=255)                                                                                                            aTGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaa                                       <  1:1082671/151‑1 (MQ=255)                                                                                                              gCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCTGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaa                                      <  2:626551/150‑1 (MQ=255)                                                                                                              gCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaa                                      >  2:593513/1‑150 (MQ=255)                                                                                                              gCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaa                                      <  1:492208/150‑1 (MQ=255)                                                                                                                 tttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACg                                  >  2:912599/1‑151 (MQ=255)                                                                                                                  ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACg                                  <  1:1228910/150‑1 (MQ=255)                                                                                                                  ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACg                                  <  2:791394/150‑1 (MQ=255)                                                                                                                   tCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACgg                                 <  2:430149/150‑1 (MQ=255)                                                                                                                    cacaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGa                                <  1:746324/150‑1 (MQ=255)                                                                                                                     acaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAtt                              >  1:572372/1‑151 (MQ=255)                                                                                                                          gCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                         <  2:266400/151‑1 (MQ=255)                                                                                                                          gCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                         <  1:600499/151‑1 (MQ=255)                                                                                                                            gACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                         >  1:10070/1‑149 (MQ=255)                                                                                                                            gACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAg                       <  1:132823/151‑1 (MQ=255)                                                                                                                            gACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAg                       <  1:389053/151‑1 (MQ=255)                                                                                                                                aaCAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGAtt                   >  1:1238458/1‑151 (MQ=255)                                                                                                                                  cAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtct                 <  1:813143/151‑1 (MQ=255)                                                                                                                                  cAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtct                 >  2:733227/1‑151 (MQ=255)                                                                                                                                  cAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtct                 >  1:721661/1‑151 (MQ=255)                                                                                                                                   aGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtctc                <  2:476459/151‑1 (MQ=255)                                                                                                                                   aGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtctc                <  2:1121771/151‑1 (MQ=255)                                                                                                                                   aGGAAGTTTCAGCGCCAGCTCGGGGGTTGCGGGACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAtggaagaaggaggata                <  2:322917/151‑16 (MQ=255)                                                                                                                                    ggAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTctct               >  1:1270255/1‑151 (MQ=255)                                                                                                                                    ggAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTctct               <  1:14700/151‑1 (MQ=255)                                                                                                                                     gAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCtt              <  2:443532/151‑1 (MQ=255)                                                                                                                                       aGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAg            <  1:58855/151‑1 (MQ=255)                                                                                                                                       aGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAg            <  1:949004/151‑1 (MQ=255)                                                                                                                                            cAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTggg        <  1:758833/150‑1 (MQ=255)                                                                                                                                            cAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg         >  2:1065740/1‑149 (MQ=255)                                                                                                                                             aGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTggg        >  1:1159288/1‑149 (MQ=255)                                                                                                                                             aGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa      <  2:553130/151‑1 (MQ=255)                                                                                                                                             aGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa      <  1:951631/151‑1 (MQ=255)                                                                                                                                               cgcCAGCGCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa      <  2:1276362/149‑1 (MQ=255)                                                                                                                                                gcCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAagag   <  2:940369/151‑1 (MQ=255)                                                                                                                                                 ccAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAgg  <  2:166112/151‑1 (MQ=255)                                                                                                                                                  cAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAgg  >  1:618753/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGG  >  NC_000913/11516‑11810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑