Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	473,636	T→G	F224C (TTC→TGC)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	473,636	0	T	G	100.0%	258.8 / NA	67	F224C (TTC→TGC)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/35);  total (32/35)

GGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTTCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGG  >  NC_000913/473490‑473782                                                                                                                                                   |                                                                                                                                                   ggCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCAcc                                                                                                                                                <  1:703934/151‑1 (MQ=255)     tctcACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCgg                                                                                                                                              <  2:313731/149‑1 (MQ=255)     tctcACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGAGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATATGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCggg                                                                                                                                             >  2:797331/1‑150 (MQ=255)        cACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACt                                                                                                                                          <  2:1140825/150‑1 (MQ=255)        cACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACt                                                                                                                                          <  2:649165/150‑1 (MQ=255)        cACGGTGCGCGGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACt                                                                                                                                          <  1:994459/150‑1 (MQ=255)         aCGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGc                                                                                                                                        <  1:305399/151‑1 (MQ=255)             tGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCAtt                                                                                                                                    >  2:1194659/1‑151 (MQ=255)                gcTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATtct                                                                                                                                  <  2:213828/150‑1 (MQ=255)                     aTTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTatat                                                                                                                            <  2:1298131/151‑1 (MQ=255)                          gcgGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCggtt                                                                                                                       >  2:179581/1‑151 (MQ=255)                          gcgGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCggtt                                                                                                                       >  2:1127869/1‑151 (MQ=255)                            gggTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCggttgg                                                                                                                     >  2:427743/1‑151 (MQ=255)                            gggTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCggttgg                                                                                                                     <  1:871628/151‑1 (MQ=255)                                ggCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGttt                                                                                                                  <  2:75008/150‑1 (MQ=255)                                   aCCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCt                                                                                                              >  2:429124/1‑151 (MQ=255)                                     cGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCttt                                                                                                            <  2:181574/151‑1 (MQ=255)                                      gtggtgCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTa                                                                                                           <  1:902394/151‑1 (MQ=255)                                        ggtgCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTaa                                                                                                          <  2:1038088/150‑1 (MQ=255)                                        ggtgCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAAc                                                                                                         >  2:620575/1‑151 (MQ=255)                                         gtgCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTAGTTTGGCTTTAACg                                                                                                        <  1:793593/151‑1 (MQ=255)                                              caTTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCggg                                                                                                   >  1:937347/1‑151 (MQ=255)                                               aTTAACGCCGCATTCGCCGGTCTGGTGGGCGCGTGTCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCggg                                                                                                   <  2:492262/150‑1 (MQ=255)                                                  agcgccgcAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCa                                                                                                <  1:115510/148‑1 (MQ=255)                                                  aaCGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTc                                                                                                 >  2:342674/1‑149 (MQ=255)                                                  aaCGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCa                                                                                                <  2:587551/150‑1 (MQ=255)                                                             cGCCGGTCTGGAGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCg                                                                                    >  1:24190/1‑151 (MQ=255)                                                              gCCGGTATGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACgg                                                                                      >  1:743038/1‑148 (MQ=255)                                                               ccGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGaa                                                                                  <  2:695245/151‑1 (MQ=255)                                                                cGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAAt                                                                                 >  2:535298/1‑151 (MQ=255)                                                                 ggTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACACCCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAAt                                                                                 <  1:384638/150‑1 (MQ=255)                                                                     tggtggGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAAt                                                                            >  1:200307/1‑151 (MQ=255)                                                                      ggtggGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATc                                                                           <  2:364926/151‑1 (MQ=255)                                                                         gggCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATcgcg                                                                        <  2:1249101/151‑1 (MQ=255)                                                                           gcgcGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGc                                                                      <  2:1040744/151‑1 (MQ=255)                                                                             gcgTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCAc                                                                    >  2:1126209/1‑151 (MQ=255)                                                                                 aTCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTgg                                                                 <  2:193468/150‑1 (MQ=255)                                                                                   cTGATAGGAAAACGCGTGGGCATCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGACAGCGGGTACGGCGAATGAAATCGCGGTACTg                                                                  >  1:1217054/1‑147 (MQ=255)                                                                                     gATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCAttt                                                            >  1:344286/1‑151 (MQ=255)                                                                                     gATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCAttt                                                            >  1:171112/1‑151 (MQ=255)                                                                                     gATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCAttt                                                            <  1:124552/151‑1 (MQ=255)                                                                                        aGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTtgt                                                          <  2:1038109/150‑1 (MQ=255)                                                                                         ggAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTtgtg                                                         >  2:1025899/1‑150 (MQ=255)                                                                                          gAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGa                                                        >  2:887685/1‑150 (MQ=255)                                                                                              aCGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACt                                                   >  2:1017872/1‑151 (MQ=255)                                                                                               cgcgTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACt                                                   <  1:1236949/150‑1 (MQ=255)                                                                                                gcgTGGGCTTCGGTAAAGAGGCGCTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACtgt                                                 >  1:759330/1‑151 (MQ=255)                                                                                                  gTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTgg                                               >  1:547746/1‑151 (MQ=255)                                                                                                  gTGGGCTTCGGTAAAGAGGCGTTTAAACCGAACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTgg                                               <  2:715231/151‑1 (MQ=255)                                                                                                        ttCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCa                                          >  1:643684/1‑150 (MQ=255)                                                                                                         tCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAAc                                        >  1:290934/1‑151 (MQ=255)                                                                                                           ggTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAAcgg                                      >  1:175948/1‑151 (MQ=255)                                                                                                              aaaGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAAcggcgg                                   >  2:431812/1‑151 (MQ=255)                                                                                                               aaGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAAcggcgg                                   >  1:702388/1‑150 (MQ=255)                                                                                                                 gagGCGTTTAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAAcggcggcgg                                <  2:1176340/151‑1 (MQ=255)                                                                                                                        ttAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTc                          <  1:943974/150‑1 (MQ=255)                                                                                                                        ttAAACCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCt                         >  2:868785/1‑151 (MQ=255)                                                                                                                           aaCCGCACAACCTGCCGCTGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGTGGCACTGGAATTTGTGACTACTGTGGTCCCAACGGCGACGGGAATTCt                         >  1:193720/1‑148 (MQ=255)                                                                                                                           aaCCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTgg                      >  2:384273/1‑151 (MQ=255)                                                                                                                            aCCGCACAACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGc                     <  2:50287/151‑1 (MQ=255)                                                                                                                                cacaACCTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTgg                  <  1:654235/150‑1 (MQ=255)                                                                                                                                      cTGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGACGGCCATTCTTGGCTGGATCTTCg           >  2:66134/1‑151 (MQ=255)                                                                                                                                       tGCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCgg          >  1:344967/1‑151 (MQ=255)                                                                                                                                        gCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCgg          <  1:480271/150‑1 (MQ=255)                                                                                                                                        gCCGATGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCg           <  2:1065906/149‑1 (MQ=255)                                                                                                                                            aTGGTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATATTCGGTg        <  2:539268/148‑1 (MQ=255)                                                                                                                                               gTCTGCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATggg  <  2:1081907/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTGCCGATGGTCTTCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGGTCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGG  >  NC_000913/473490‑473782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑