Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	622,725	A→C	E142D (GAA→GAC)	entS →	enterobactin exporter, iron‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	622,725	0	A	C	100.0%	325.8 / NA	86	E142D (GAA→GAC)	entS	enterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (44/42);  total (44/42)

GGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGC  >  NC_000913/622582‑622870                                                                                                                                                |                                                                                                                                                  ggCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGTGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTa                                                                                                                                             >  2:378393/1‑150 (MQ=255) ggCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTaa                                                                                                                                            <  2:1028305/151‑1 (MQ=255)      cATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAAt                                                                                                                                           >  1:47508/1‑147 (MQ=255)      cATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAg                                                                                                                                       <  2:800823/151‑1 (MQ=255)       aTTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAgg                                                                                                                                      >  2:959591/1‑151 (MQ=255)       aTTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAgg                                                                                                                                      <  1:600946/151‑1 (MQ=255)          ggACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCg                                                                                                                                   <  2:1071107/151‑1 (MQ=255)          ggACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCg                                                                                                                                   <  2:966099/151‑1 (MQ=255)           gACTGTGCCTTAATGCACTGCTGCCGGATCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCgg                                                                                                                                  <  2:95437/151‑1 (MQ=255)             cTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCgggg                                                                                                                                >  1:662998/1‑151 (MQ=255)               gtgCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGc                                                                                                                               <  2:527062/150‑1 (MQ=255)               gtgCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCg                                                                                                                              >  1:950698/1‑151 (MQ=255)               gtgCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCg                                                                                                                              >  1:703845/1‑151 (MQ=255)                  ccTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATc                                                                                                                           >  1:190770/1‑151 (MQ=255)                    ttAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGGGGGACCACAACCAGTAGGGGGGGACAACCTAATGTAAGGCgggg                                                                                                                                >  1:938596/1‑144 (MQ=255)                    ttAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACAGCAGCACTGGCAGGGCGCGCCAACTTAATGCAGGCCGGGGCGATCAc                                                                                                                         >  1:1200417/1‑151 (MQ=255)                     tAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCAcc                                                                                                                        >  2:411080/1‑151 (MQ=255)                              gctgcCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGAcc                                                                                                               >  2:227898/1‑151 (MQ=255)                               ctgcCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGCCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGAGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCg                                                                                                              >  2:610447/1‑151 (MQ=255)                                        ccGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTgg                                                                                                     >  1:334736/1‑151 (MQ=255)                                         cGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTggg                                                                                                    >  1:950504/1‑151 (MQ=255)                                         cGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTggg                                                                                                    <  2:1179774/151‑1 (MQ=255)                                          gTCATTGCTGGCAATCTATTTTCTTGGTTTATGGGATGGTTTTGTCGCATCGCTTGTCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTgggg                                                                                                   <  2:1182444/151‑1 (MQ=255)                                          gTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTgggg                                                                                                   <  1:1236102/151‑1 (MQ=255)                                           tCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTgggg                                                                                                   >  2:467852/1‑150 (MQ=255)                                            cATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGGTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTc                                                                                                 <  2:692868/151‑1 (MQ=255)                                            cATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTc                                                                                                 <  1:955520/151‑1 (MQ=255)                                            cATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTc                                                                                                 >  2:832654/1‑151 (MQ=255)                                            cATTGCTGGCAATCTATTTACTTGGTGTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTc                                                                                                 <  1:136746/151‑1 (MQ=255)                                              ttGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCgg                                                                                               >  1:954309/1‑151 (MQ=255)                                                   ggCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGa                                                                                            <  2:497808/149‑1 (MQ=255)                                                     cAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTc                                                                                        >  2:368573/1‑151 (MQ=255)                                                      aaTCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTc                                                                                        >  2:1234810/1‑150 (MQ=255)                                                       aTCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGc                                                                                      >  1:246180/1‑151 (MQ=255)                                                           aTTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGcc                                                                                     <  2:778098/148‑1 (MQ=255)                                                           aTTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCAt                                                                                  >  2:1032579/1‑151 (MQ=255)                                                           aTTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCAt                                                                                  <  1:888663/151‑1 (MQ=255)                                                            tttACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATg                                                                                 >  1:934796/1‑151 (MQ=255)                                                               aCTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAt                                                                               <  1:1074964/150‑1 (MQ=255)                                                                   ggTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCg                                                                          <  1:1079273/151‑1 (MQ=255)                                                                     tttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCgg                                                                         >  1:1239927/1‑150 (MQ=255)                                                                       tATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGttt                                                                      >  1:424894/1‑151 (MQ=255)                                                                       tATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGttt                                                                      >  1:41990/1‑151 (MQ=255)                                                                          gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTa                                                                     <  1:903351/149‑1 (MQ=255)                                                                          gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAt                                                                    <  1:356745/150‑1 (MQ=255)                                                                          gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAt                                                                    <  1:392820/150‑1 (MQ=255)                                                                           ggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAtt                                                                   >  2:69474/1‑150 (MQ=255)                                                                           ggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAt                                                                    <  2:497567/149‑1 (MQ=255)                                                                           ggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATAATTGGCGGTTTATTg                                                                  <  2:1201627/151‑1 (MQ=255)                                                                              tGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTg                                                               >  2:864593/1‑151 (MQ=255)                                                                               ggTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTgg                                                              <  2:640181/151‑1 (MQ=255)                                                                                 ttttttCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCg                                                            >  2:1259481/1‑151 (MQ=255)                                                                                  tttttCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCg                                                            >  2:1165395/1‑150 (MQ=255)                                                                                     ttCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGAcc                                                         <  2:215782/150‑1 (MQ=255)                                                                                       cGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGGCAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCCTGATTGGCGGGTTATTGTTGGCGAcccg                                                       >  1:1043747/1‑148 (MQ=255)                                                                                          aTCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTgg                                                    >  2:1009152/1‑150 (MQ=255)                                                                                             gCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTTTTGCTGGCGACCGGTGGCg                                                  >  1:588192/1‑149 (MQ=255)                                                                                             gCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGGGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGt                                                 >  1:86913/1‑150 (MQ=255)                                                                                              cTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGt                                                 >  2:1134744/1‑149 (MQ=255)                                                                                                tGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAg                                               <  2:1194422/149‑1 (MQ=255)                                                                                                 ggCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCt                                            <  2:555923/151‑1 (MQ=255)                                                                                                    gTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGGTTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTgg                                          >  2:174128/1‑150 (MQ=255)                                                                                                    gTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGGGGCGTAGCCTgg                                          >  1:667863/1‑150 (MQ=255)                                                                                                     ttACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGGGGCGGAGCCTgg                                          >  1:410197/1‑149 (MQ=255)                                                                                                       aCGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACt                                      <  1:757619/151‑1 (MQ=255)                                                                                                        cGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACt                                      >  2:715714/1‑150 (MQ=255)                                                                                                          gcgcTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACg                                   <  2:1138238/151‑1 (MQ=255)                                                                                                              tATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGc                                >  2:128911/1‑150 (MQ=255)                                                                                                                 tGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggc                            >  2:585860/1‑151 (MQ=255)                                                                                                                  ggcggcGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcg                           <  1:434785/151‑1 (MQ=255)                                                                                                                    cggcgACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcggc                         <  1:986307/151‑1 (MQ=255)                                                                                                                        gACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGgcggcggcg                     <  2:1116225/151‑1 (MQ=255)                                                                                                                         acacCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGcggcggcgg                    <  1:996297/151‑1 (MQ=255)                                                                                                                              aGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACg               >  2:188923/1‑151 (MQ=255)                                                                                                                                cACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGtt             >  2:1266260/1‑151 (MQ=255)                                                                                                                                cACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGtt             <  2:912022/151‑1 (MQ=255)                                                                                                                                 aatGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGAGGCGGGCACGttt            <  1:1193752/149‑1 (MQ=255)                                                                                                                                 aCTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCa                 <  1:141317/146‑1 (MQ=255)                                                                                                                                    ggTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatt         <  1:1234734/151‑1 (MQ=255)                                                                                                                                    ggTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatt         <  1:610428/151‑1 (MQ=255)                                                                                                                                    ggTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTtta           <  1:1000273/149‑1 (MQ=255)                                                                                                                                     gTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatta        <  1:143910/151‑1 (MQ=255)                                                                                                                                      tAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatta        >  2:940529/1‑150 (MQ=255)                                                                                                                                         ggCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCtt    <  2:649532/151‑1 (MQ=255)                                                                                                                                           cGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTg   <  2:1157733/150‑1 (MQ=255)                                                                                                                                            gTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCCGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGc  >  1:897296/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                  GGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGC  >  NC_000913/622582‑622870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑