Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,662 A→C intergenic (+257/‑44) sdhB → / → sucA succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,6620AC99.1% 423.1 / ‑4.2 106intergenic (+257/‑44)sdhB/sucAsuccinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (52/53);  total (53/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

Only 100 of 109 total aligned reads displayed.

CGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTG  >  NC_000913/758517‑758808
                                                                                                                                                    |                                                                                                                                                  
cgcaTCGGGCGTAAAAAAGAAATGCAGGAAATCTTTAAAAACGGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCAc                                                                                                                                               <  2:449670/151‑1 (MQ=255)
   aTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGc                                                                                                                                            >  2:7756/1‑151 (MQ=255)
      aGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGa                                                                                                                                          >  2:821916/1‑150 (MQ=255)
       ggtaaaaaaaaaaaATGAAGAAAATCTTAAAACACTCCCCCCGACCCTAAGACATTTTTAAATGGTTCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAg                                                                                                                                        <  1:1284/143‑1 (MQ=255)
       ggcagaaaaaaGAAATGCAGAAATTCTTAAAAACCTGCCCCTGACAAGAAGACATTTTTTAAAGCTTCCGTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGaa                                                                                                                                         <  1:817371/142‑1 (MQ=255)
       ggCGAAAAAAAAAAATGCAGAAAATTTTAAAAACCTCCCCCTGACACAAAGACAGTTTTTAAAGGTTCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCg                                                                                                                                           <  1:1237793/148‑1 (MQ=255)
         cGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGt                                                                                                                                       >  2:130614/1‑150 (MQ=255)
            aaaaaaGAAATGGAGAAAATCTTAAAAAACGCCCCCTGACACTAAGACATTTTTAAAAGTTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTaa                                                                                                                                     <  2:829524/146‑1 (MQ=255)
            aaCAAAGAAATGCAGAAAATCTTAAAAACCTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTa                                                                                                                                      <  2:910084/148‑1 (MQ=255)
             aaaaagaaagGCAGGAAATCTTAAAAAACCGCCCCTGACACTAAGACGTTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAATCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCa                                                                                                                                  <  1:1157441/149‑1 (MQ=255)
             aCAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGc                                                                                                                                   >  1:210023/1‑150 (MQ=255)
              aaaaaaaATGCAGAAAATCTTAAAAACCTCCCCCTGACACTAAGACAGTTTTAAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCAt                                                                                                                                 <  1:1201689/150‑1 (MQ=255)
               aaaGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCAt                                                                                                                                 >  1:255331/1‑150 (MQ=255)
                aaGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATa                                                                                                                                >  2:938076/1‑150 (MQ=255)
                   aagtGCGGGAAATCTTTAAAACCGCCCCCGGCCAAAAAGAAATTTTTAAAGGTTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATaaaaa                                                                                                                            <  1:356186/148‑1 (MQ=255)
                   aaaTGCAGAAAATTTTTAAAAACTGCCCCTGACACAAAGACAGTTTTTAAAGTTCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATaaaa                                                                                                                             <  2:159424/150‑1 (MQ=255)
                   aaaTGCAGAAAATCTTAAAAAACTGCCCCTGACACAAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATaaaaa                                                                                                                            <  2:817566/151‑1 (MQ=255)
                      tGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATaaaaa                                                                                                                            >  1:924668/1‑148 (MQ=255)
                      tGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATaaaaa                                                                                                                            >  2:774334/1‑148 (MQ=255)
                      tGCAGGAAATCTTAAAAAACTCCCCCTGACACTAAGACAGTTTTAAAAGGTCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                          <  1:526134/150‑1 (MQ=255)
                        ggagAAAACTTTAAAAAACTCCCCCTACCACAAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGa                                                                                                                         <  2:439318/147‑1 (MQ=255)
                        cAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATg                                                                                                                       >  2:912632/1‑151 (MQ=255)
                        cAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATg                                                                                                                       >  2:231279/1‑151 (MQ=255)
                            aaaTTTTAAAAACCCCCCCCTGACACTAAGAAATTTTTAAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCtt                                                                                                                    <  1:977892/150‑1 (MQ=255)
                             aaattttAAAAACCGCCCCCGGACACAAAGACAGTTTTAAAGGGTCCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTa                                                                                                                   <  2:261764/147‑1 (MQ=255)
                                cTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAggg                                                                                                               >  2:152288/1‑151 (MQ=255)
                                 tttAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAggg                                                                                                               >  2:1300481/1‑150 (MQ=255)
                                 tttAAAAACTGCCCCTGAAACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAggg                                                                                                               >  1:861312/1‑150 (MQ=255)
                                     aaaaaccccccccTTACACAAGGAAAGTTTTTAAAGGTTCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAgg                                                                                                                <  1:1077367/138‑1 (MQ=255)
                                     aaaaaCTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATc                                                                                                            <  2:516743/150‑1 (MQ=255)
                                       aaaccgcccccTGACACTAAGACAGTTTTAAAGGGTCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCAc                                                                                                          <  1:1223086/144‑1 (MQ=255)
                                       aaaccccccccTGACACAAAGACAGTTTTTAAAGGTCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCAc                                                                                                          <  1:975201/144‑1 (MQ=255)
                                         aCTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGAt                                                                                                       >  1:217105/1‑151 (MQ=255)
                                              ccccTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGc                                                                                                     >  1:181076/1‑149 (MQ=255)
                                              ccccTGACACAAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATg                                                                                                      <  2:797091/148‑1 (MQ=255)
                                               cccTGACACTAAGACAGTTTTAAAAGTTCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGa                                                                                                  <  2:663916/151‑1 (MQ=255)
                                               cccTGACACAAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGa                                                                                                  <  1:185828/151‑1 (MQ=255)
                                                ccTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGa                                                                                                  >  2:1139498/1‑150 (MQ=255)
                                                ccTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGa                                                                                                  >  1:391952/1‑150 (MQ=255)
                                                  tGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAAc                                                                                                >  1:593986/1‑150 (MQ=255)
                                                     cacTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAgcg                                                                                            >  1:948291/1‑151 (MQ=255)
                                                      acTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAgcgc                                                                                           >  2:438156/1‑151 (MQ=255)
                                                       cTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCt                                                                                          >  2:145456/1‑151 (MQ=255)
                                                       cTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCt                                                                                          >  2:1022487/1‑151 (MQ=255)
                                                           gctctttttaAAAGGCCCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGa                                                                                      <  1:614263/146‑1 (MQ=255)
                                                                  tttAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTg                                                                               >  2:1272177/1‑151 (MQ=255)
                                                                  tttAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTg                                                                               >  2:728966/1‑151 (MQ=255)
                                                                     aagggTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCt                                                                                <  1:836264/144‑1 (MQ=255)
                                                                     aagggTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTgg                                                                              <  2:214335/146‑1 (MQ=255)
                                                                     aaaGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGtt                                                                            >  1:82301/1‑151 (MQ=255)
                                                                           ttCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGa                                                                         <  2:679295/149‑1 (MQ=255)
                                                                            tCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGActc                                                                      >  1:1100300/1‑151 (MQ=255)
                                                                             ccgtCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGAct                                                                       <  1:106829/146‑1 (MQ=255)
                                                                             ccTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGActct                                                                     >  1:953245/1‑151 (MQ=255)
                                                                             ccTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGActc                                                                      <  2:727406/150‑1 (MQ=255)
                                                                              cTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACtctt                                                                    >  1:1083040/1‑151 (MQ=255)
                                                                              cTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACtctt                                                                    >  1:862180/1‑151 (MQ=255)
                                                                               ttCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACtcttc                                                                   >  1:418236/1‑151 (MQ=255)
                                                                               ttCCCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGActc                                                                      <  1:59889/148‑1 (MQ=255)
                                                                                  gcgAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACtcttct                                                                  <  2:110553/149‑1 (MQ=255)
                                                                                  gcgAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTcttctt                                                                 >  1:39244/1‑150 (MQ=255)
                                                                                       ccACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACctct                                                           >  1:747548/1‑151 (MQ=255)
                                                                                        cACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACctctc                                                          >  1:389672/1‑151 (MQ=255)
                                                                                         aCTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACctctct                                                         <  2:937954/151‑1 (MQ=255)
                                                                                          cTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTg                                                        >  1:264576/1‑151 (MQ=255)
                                                                                             cGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGgcg                                                     >  2:72529/1‑151 (MQ=255)
                                                                                             cGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGgcg                                                     >  1:258046/1‑151 (MQ=255)
                                                                                             cGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGgcg                                                     <  1:580536/151‑1 (MQ=255)
                                                                                                aGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCaa                                                  <  2:487750/151‑1 (MQ=255)
                                                                                                aGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCa                                                   <  1:969581/150‑1 (MQ=255)
                                                                                                      gagCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAAcc                                               >  1:173909/1‑148 (MQ=255)
                                                                                                        gCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGc                                          >  2:943288/1‑151 (MQ=255)
                                                                                                         cTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCt                                         >  2:121769/1‑151 (MQ=255)
                                                                                                           cGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCt                                         <  2:624278/149‑1 (MQ=255)
                                                                                                           cGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTgg                                       <  2:416893/151‑1 (MQ=255)
                                                                                                              aaGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGAt                                     >  2:976871/1‑150 (MQ=255)
                                                                                                               aGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAg                                   <  1:1020004/151‑1 (MQ=255)
                                                                                                                gTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGAt                                     >  1:1157218/1‑148 (MQ=255)
                                                                                                                gTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGAt                                     >  1:560565/1‑148 (MQ=255)
                                                                                                                gTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGa                                  <  1:306042/151‑1 (MQ=255)
                                                                                                                    aCCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAAc                                >  1:357035/1‑149 (MQ=255)
                                                                                                                    aCCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAg                              <  2:521787/151‑1 (MQ=255)
                                                                                                                    aCCCCGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAg                              <  1:436379/151‑1 (MQ=255)
                                                                                                                     ccccGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGc                             >  2:334104/1‑151 (MQ=255)
                                                                                                                      cccGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGct                            <  2:202173/151‑1 (MQ=255)
                                                                                                                       ccGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGctc                           >  2:76987/1‑151 (MQ=255)
                                                                                                                        cGGCACGCACATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGctct                          <  2:302000/151‑1 (MQ=255)
                                                                                                                            acgcacATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATg                       >  1:1260074/1‑150 (MQ=255)
                                                                                                                            acgcacATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATg                       >  1:1017547/1‑150 (MQ=255)
                                                                                                                            acgcacATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGa                      <  1:527291/151‑1 (MQ=255)
                                                                                                                             cgcacATCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGaa                     <  2:956803/151‑1 (MQ=255)
                                                                                                                                 caTCACTGTGCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGTCTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGAct                 >  2:747214/1‑151 (MQ=255)
                                                                                                                                    cACTGTGCGTGTTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGActtct              <  1:910157/151‑1 (MQ=255)
                                                                                                                                       tgtgCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTa            >  2:709778/1‑150 (MQ=255)
                                                                                                                                         ttcGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCTCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTa            <  2:1116403/146‑1 (MQ=255)
                                                                                                                                          gCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAcc         <  1:1300400/150‑1 (MQ=255)
                                                                                                                                          gCGTGGTAGTCTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCg        <  2:747575/151‑1 (MQ=255)
                                                                                                                                               gtagtcTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCt   <  2:829407/151‑1 (MQ=255)
                                                                                                                                               gtagtcTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCt   >  2:285281/1‑151 (MQ=255)
                                                                                                                                                 agtcTCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTg  <  2:747907/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGACTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTG  >  NC_000913/758517‑758808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: