Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,040,032 T→G L246R (CTG→CGG)  cbdB → cytochrome bd‑II oxidase, subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,040,0320TG98.5% 215.4 / ‑3.7 66L246R (CTG→CGG) cbdBcytochrome bd‑II oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (31/34);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

ATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATC  >  NC_000913/1039885‑1040179
                                                                                                                                                    |                                                                                                                                                   
aTCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACggg                                                                                                                                                   >  2:356968/1‑150 (MQ=255)
   tGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGGTCGGATTTATGGCTTTGTACTGCTCGCCCCGGATGCCAACGGTCGTTCCAATTCGGTAATGAAACgggggg                                                                                                                                                >  1:170906/1‑150 (MQ=255)
    gCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCa                                                                                                                                              <  2:299362/151‑1 (MQ=255)
         cacaGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCt                                                                                                                                         <  1:87218/151‑1 (MQ=255)
               tGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGGTACTGGCTGGGGGTCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGGTAATGAAACGGGGGGCCGTGCTACCTgg                                                                                                                                   >  1:268049/1‑151 (MQ=255)
                  cGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGACGGTATTGATGGGTTTATACTGCTCCCCCAGGATGCTAAAGGTCCTTCCAATCCGTTAAAGAAACGGGTGGCAGTGCTACCTgggg                                                                                                                                 >  2:1133957/1‑148 (MQ=255)
                    aCCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGGGGGGCGGCATTGATGGCTTTGTACTGCTCGGCCAGGATGCTAACGGGCCCTCCCATCCGTTAATGAAACGGGGGGGAGGGCTACCCGGGGCCt                                                                                                                              >  2:1075108/1‑151 (MQ=255)
                     ccAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTg                                                                                                                             <  1:900528/151‑1 (MQ=255)
                         aCGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATg                                                                                                                         <  2:245315/151‑1 (MQ=255)
                          cgcgCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATg                                                                                                                         <  2:481815/150‑1 (MQ=255)
                           gcgcTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGaa                                                                                                                       <  1:913453/151‑1 (MQ=255)
                             gcTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTAACTGGTGGCTGGATGaat                                                                                                                      >  2:1022/1‑150 (MQ=255)
                                 cACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGGATTGATGGCTTTTTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAAAGGGGGGCAGTGCTACCTGGGGCCTGGATGaataat                                                                                                                   >  1:820638/1‑149 (MQ=255)
                                   cTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGGATTGATGGCTTTTTTCTGCTCGCCCCGGATGCTAACGGTCCTTTCAATCCGTTAATGAAACGGGGGGCAGGGCTACCTGGTGCCTGGATGAATAAtttt                                                                                                                >  1:147829/1‑150 (MQ=255)
                                    tGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCCGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGGAGTGGTACCTGGTGCCTGGATGAAAAAtttt                                                                                                                >  2:942773/1‑149 (MQ=255)
                                          tGATGCTGTGCTTTTTGCTGGCGGGTTTCTGGGTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAAGGTGCTAATGGTCCTTCCTATCCGTTAATGAAACGGGGGGCAGTGCTACCTGGGGCCTGGATGAAAAATTTTGTCggg                                                                                                          >  2:306959/1‑147 (MQ=255)
                                           gATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCg                                                                                                       <  2:808078/151‑1 (MQ=255)
                                           gATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCg                                                                                                       >  2:140392/1‑151 (MQ=255)
                                           gATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCg                                                                                                       <  1:1009402/151‑1 (MQ=255)
                                            aTGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGAGGGGCGGTATTGATGGGCTTGTACTGCTCGCCCCGGATGCTTACGGTTCTTCCAATTCGTTAATGTAAAGGGTGGCAGGGCGAACGGGGGCCGGGAGGAATCATTTTGTCGGGtt                                                                                                        >  1:83468/1‑148 (MQ=255)
                                                 gtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGtt                                                                                                 <  1:111695/151‑1 (MQ=255)
                                                    cTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGGGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGtttt                                                                                               >  1:1222501/1‑150 (MQ=255)
                                                    cTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGTATTGATGGCTTTTTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTTATGAAACGGGGGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGtttt                                                                                               >  1:1147583/1‑150 (MQ=255)
                                                     tttttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTTTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGtttt                                                                                               >  2:953045/1‑149 (MQ=255)
                                                      ttttGCTGGCGGGTTACTGGTTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTtg                                                                                              <  2:1291041/149‑1 (MQ=255)
                                                       tttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTtgtg                                                                                            <  2:609739/150‑1 (MQ=255)
                                                        ttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTgg                                                                                           <  2:188654/150‑1 (MQ=255)
                                                         tGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGGGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGa                                                                                          >  2:978158/1‑150 (MQ=255)
                                                          gCTGGCGGGTTACTGGCTGTGGGTCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAAAGGGGGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTgg                                                                                           >  1:323400/1‑148 (MQ=255)
                                                               cGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTccc                                                                                   >  2:293401/1‑151 (MQ=255)
                                                                gggTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                                  >  1:1298568/1‑151 (MQ=255)
                                                                gggTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                                  >  1:1180298/1‑151 (MQ=255)
                                                                 ggTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgc                                                                                 <  2:540689/151‑1 (MQ=255)
                                                                     aCTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgctgct                                                                             >  1:608897/1‑151 (MQ=255)
                                                                     aCTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgctg                                                                               <  1:198376/149‑1 (MQ=255)
                                                                            gtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTTTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAtt                                                                       <  1:806155/150‑1 (MQ=255)
                                                                                ggTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                                   >  2:164810/1‑151 (MQ=255)
                                                                                ggTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                                   >  2:1234857/1‑151 (MQ=255)
                                                                                 gTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATtcttct                                                                  <  1:621092/151‑1 (MQ=255)
                                                                                 gTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATtcttct                                                                  <  1:1034179/151‑1 (MQ=255)
                                                                                   cGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGc                                                                >  2:1212058/1‑151 (MQ=255)
                                                                                     gTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                                              >  1:834684/1‑151 (MQ=255)
                                                                                       aTTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCAt                                                            <  1:1278958/151‑1 (MQ=255)
                                                                                             ggCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTg                                                       >  1:1082523/1‑150 (MQ=255)
                                                                                                     aCTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATg                                              <  2:251920/151‑1 (MQ=255)
                                                                                                           cGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGa                                          <  2:1274406/149‑1 (MQ=255)
                                                                                                           cGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGAt                                         <  1:67577/150‑1 (MQ=255)
                                                                                                            gCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGAttt                                       >  1:784880/1‑151 (MQ=255)
                                                                                                             cccAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGAttt                                       <  1:499329/150‑1 (MQ=255)
                                                                                                              taaGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGAttt                                       >  1:250754/3‑149 (MQ=255)
                                                                                                               cAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATc                                    >  2:48465/1‑151 (MQ=255)
                                                                                                                     gCTAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGt                               <  1:502473/150‑1 (MQ=255)
                                                                                                                        aaCGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGc                            <  1:586161/150‑1 (MQ=255)
                                                                                                                         aCGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGccc                          <  2:1280281/151‑1 (MQ=255)
                                                                                                                            gTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCggg                       <  2:1291643/151‑1 (MQ=255)
                                                                                                                                ttCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTgg                   >  1:557950/1‑151 (MQ=255)
                                                                                                                                 tCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTgg                   <  2:893940/150‑1 (MQ=255)
                                                                                                                                   cAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTgggg                 >  2:146570/1‑150 (MQ=255)
                                                                                                                                    aaTCCCTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGCTCGCCCGGGTTggg                  <  1:377408/148‑1 (MQ=255)
                                                                                                                                       ccGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGAtttt            >  2:769220/1‑151 (MQ=255)
                                                                                                                                       ccGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGAttt             <  2:1230218/150‑1 (MQ=255)
                                                                                                                                         gTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTg          >  1:133861/1‑151 (MQ=255)
                                                                                                                                            aaTGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTTTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGAt        <  1:276670/150‑1 (MQ=255)
                                                                                                                                            aaTGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATg       <  1:1242899/151‑1 (MQ=255)
                                                                                                                                             aTGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATgg      <  1:493870/151‑1 (MQ=255)
                                                                                                                                              tGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATg       <  1:513296/149‑1 (MQ=255)
                                                                                                                                                aaaCGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGcat   <  1:690536/151‑1 (MQ=255)
                                                                                                                                                 aaCGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGcatc  >  2:875607/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATC  >  NC_000913/1039885‑1040179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: