Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,188,512	A→C	L422* (TTA→TGA)	phoQ ←	sensory histidine kinase in two‑component regulatory system with PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,188,512	0	A	C	100.0%	308.3 / NA	75	L422* (TTA→TGA)	phoQ	sensory histidine kinase in two‑component regulatory system with PhoP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/41);  total (34/41)

GCACCGCCCAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTAATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATCGTTCTGCTCACCG  >  NC_000913/1188368‑1188658                                                                                                                                                 |                                                                                                                                                   gcACCGCCCAGCATGCTCTCCCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGaa                                                                                                                                              <  1:722662/151‑1 (MQ=255)   aCCGCCCAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAAt                                                                                                                                             >  2:602840/1‑150 (MQ=255)    ccGCCCAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCACAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATAc                                                                                                                                           >  1:1284110/1‑151 (MQ=255)     cGCCCAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATAcc                                                                                                                                          >  1:670962/1‑151 (MQ=255)     cGCCCAGCATGCTCTCTCCGCCGACGATTTTACCCTCATATTGCTCGGGGATTTCGCGGGCTACCGCCAGCCCTACACCTGGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATa                                                                                                                                            <  1:153042/149‑1 (MQ=255)       cccAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCgg                                                                                                                                        >  1:108263/1‑151 (MQ=255)        ccAGCATGCTCTCTTCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCggg                                                                                                                                       <  1:1237708/151‑1 (MQ=255)         cAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCgggg                                                                                                                                      >  2:83259/1‑151 (MQ=255)         cAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCgggg                                                                                                                                      <  2:187220/151‑1 (MQ=255)            cATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCca                                                                                                                                   <  2:93086/151‑1 (MQ=255)            cATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCca                                                                                                                                   <  2:864414/151‑1 (MQ=255)               gCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCcatc                                                                                                                                 >  1:118685/1‑150 (MQ=255)                    ctcCGGCGACGATTTTCCCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATcc                                                                                                                             <  2:9706/149‑1 (MQ=255)                    ctcCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATcc                                                                                                                             <  1:273255/149‑1 (MQ=255)                    ctcCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTc                                                                                                                           >  1:368468/1‑151 (MQ=255)                     tcCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCg                                                                                                                          >  2:455096/1‑151 (MQ=255)                        ggCGACGATTTTACCCTCATATTGCTCGGTGTTTTCGCGGGCTGCCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGAc                                                                                                                        <  1:799636/150‑1 (MQ=255)                              gaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAat                                                                                                                  >  2:212778/1‑150 (MQ=255)                               aTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAatat                                                                                                                >  1:625767/1‑151 (MQ=255)                                 tttACCCTCATATTGCTCGGTGATTTGGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATTAACCCGTTGACCACGGTCGAAAATGGCCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCGCAAtata                                                                                                               <  1:157573/150‑1 (MQ=255)                                 tttACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAatat                                                                                                                >  2:15211/1‑149 (MQ=255)                                  ttACCCTCATATTGCTCGGTGATTTCGCGGGCTTCCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCAGGGCCATCATCCTCGACCACAAtata                                                                                                               <  1:1092297/149‑1 (MQ=255)                                  ttACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAatat                                                                                                                >  2:763676/1‑148 (MQ=255)                                   tACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATag                                                                                                              >  2:804690/1‑149 (MQ=255)                                    aCCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATagag                                                                                                            <  1:95987/150‑1 (MQ=255)                                       cTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGc                                                                                                        >  2:268356/1‑151 (MQ=255)                                         cATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCtc                                                                                                      <  1:101002/151‑1 (MQ=255)                                          atatTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCtcg                                                                                                     <  2:99430/151‑1 (MQ=255)                                          atatTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGACCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCtcg                                                                                                     <  1:1248939/151‑1 (MQ=255)                                             ttGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCtcgtcg                                                                                                  >  2:1068262/1‑151 (MQ=255)                                               gCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGt                                                                                                <  1:44164/151‑1 (MQ=255)                                                cTCGGTGATTTCGCGGGCCCCCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCtcgt                                                                                                    <  1:673832/146‑1 (MQ=255)                                                     tGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCt                                                                                          >  2:865974/1‑151 (MQ=255)                                                      gATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCtt                                                                                         <  2:637727/151‑1 (MQ=255)                                                           cgcgGGCTACCGCTAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGa                                                                                    <  1:1262565/151‑1 (MQ=255)                                                             cgGGCTACCGCCATCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGaa                                                                                   >  2:1240656/1‑150 (MQ=255)                                                              gggCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAAt                                                                                 <  2:669942/151‑1 (MQ=255)                                                               ggCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAAtt                                                                                <  2:364452/151‑1 (MQ=255)                                                                   aCCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCg                                                                             <  2:524559/150‑1 (MQ=255)                                                                     cGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACa                                                                          <  1:333656/151‑1 (MQ=255)                                                                       ccAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACGATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACaaa                                                                        <  2:1143788/151‑1 (MQ=255)                                                                        cAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACaaa                                                                        >  2:62804/1‑150 (MQ=255)                                                                         aGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACt                                                                      >  2:1194885/1‑151 (MQ=255)                                                                         aGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACt                                                                      >  2:528779/1‑151 (MQ=255)                                                                           cccTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCg                                                                    >  2:73481/1‑151 (MQ=255)                                                                             cTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCg                                                                    >  1:1299367/1‑149 (MQ=255)                                                                                cacCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACTGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCa                                                               <  2:905559/151‑1 (MQ=255)                                                                                cacCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGc                                                                >  1:1012682/1‑150 (MQ=255)                                                                                 acCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCa                                                               >  2:498445/1‑150 (MQ=255)                                                                                   cTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAat                                                             >  2:47504/1‑150 (MQ=255)                                                                                     tGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAatat                                                           <  1:346405/150‑1 (MQ=255)                                                                                      gCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATAttt                                                         <  1:883005/151‑1 (MQ=255)                                                                                       cccAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTa                                                        <  2:435812/151‑1 (MQ=255)                                                                                        ccAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTAc                                                       >  1:1163516/1‑151 (MQ=255)                                                                                           gggCGTAAAGTATCATCCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGGGGCAATATTTACAgg                                                    <  1:1105152/151‑1 (MQ=255)                                                                                            ggCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGc                                                   <  2:501829/151‑1 (MQ=255)                                                                                            ggCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGc                                                   <  2:1231197/151‑1 (MQ=255)                                                                                                 aaaGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTAt                                              <  2:449208/151‑1 (MQ=255)                                                                                                 aaaGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTAt                                              <  1:720748/151‑1 (MQ=255)                                                                                                    gTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCa                                           <  2:234194/151‑1 (MQ=255)                                                                                                         aaCCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACg                                      <  1:722254/151‑1 (MQ=255)                                                                                                          aCCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACg                                      >  2:24902/1‑150 (MQ=255)                                                                                                          aCCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGt                                     <  2:438799/151‑1 (MQ=255)                                                                                                                 gACCACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCAt                              >  1:1160529/1‑151 (MQ=255)                                                                                                                   ccACGGTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATc                             >  2:1040587/1‑150 (MQ=255)                                                                                                                       ggTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTc                        >  2:768002/1‑151 (MQ=255)                                                                                                                       ggTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTc                        <  1:880722/151‑1 (MQ=255)                                                                                                                        gTCGAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTc                        >  2:948880/1‑150 (MQ=255)                                                                                                                           gAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGAc                     >  1:314923/1‑150 (MQ=255)                                                                                                                           gAAAATGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACa                    <  1:1204263/151‑1 (MQ=255)                                                                                                                            aaaaTGACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACa                    >  2:1027626/1‑150 (MQ=255)                                                                                                                                 gACCTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATc               <  1:755079/150‑1 (MQ=255)                                                                                                                                   ccTCTCGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATCGtt            >  1:733639/1‑151 (MQ=255)                                                                                                                                        cGCTTGCTTCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATCGTTCt          <  2:568849/148‑1 (MQ=255)                                                                                                                                             gcttCATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATCGTTCTGCTCACCg  <  1:804658/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCACCGCCCAGCATGCTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTCGCTTGCTTAATGGAATACCGGGGCCATCATCCTCGACCACAATATAGAGATGCTCGTCGGTTTGCCTTGCAGAAATTTCGACAAACTCGAGGCAATATTTACAGGCATTATCCAGCACGTTGCCCATCACCTCGACAAAATCGTTCTGCTCACCG  >  NC_000913/1188368‑1188658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑