Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,348,890	A→C	L8R (CTA→CGA)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,348,890	0	A	C	98.7%	270.5 / ‑3.5	75	L8R (CTA→CGA)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (35/39);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01

ATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTAGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGC  >  NC_000913/1348745‑1349035                                                                                                                                                  |                                                                                                                                                  atgcatgACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTcgcagc                                                                                                                                              <  2:504544/151‑1 (MQ=255) atgcatgACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTcgcagc                                                                                                                                              >  2:504317/1‑151 (MQ=255)  tgcatgACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTcgcagc                                                                                                                                              <  1:827931/150‑1 (MQ=255)     atgACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGt                                                                                                                                          <  1:447058/151‑1 (MQ=255)       gACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGtt                                                                                                                                         <  1:938972/150‑1 (MQ=255)       gACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTg                                                                                                                                        >  2:1098445/1‑151 (MQ=255)        aCTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGt                                                                                                                                       >  1:112760/1‑151 (MQ=255)          ttttttCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTcc                                                                                                                                     >  1:361410/1‑151 (MQ=255)           tttttCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTc                                                                                                                                      <  2:853227/149‑1 (MQ=255)            ttttCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCt                                                                                                                                    <  2:1128721/150‑1 (MQ=255)            ttttCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTg                                                                                                                                   >  1:1276623/1‑151 (MQ=255)               tCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGaa                                                                                                                                 <  1:810531/150‑1 (MQ=255)                cATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGaaa                                                                                                                                <  1:650961/150‑1 (MQ=255)                cATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAAc                                                                                                                               >  2:48218/1‑151 (MQ=255)                   cTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACAt                                                                                                                             >  2:649636/1‑150 (MQ=255)                     gcggcggcgGAATGACATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGAGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAAt                                                                                                                          <  1:792593/151‑1 (MQ=255)                     gcggcggcgGAATGAAATATCTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATaa                                                                                                                           <  2:524369/150‑1 (MQ=255)                          ggcggAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCt                                                                                                                     >  2:953108/1‑151 (MQ=255)                            cggAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTAt                                                                                                                   >  2:497750/1‑151 (MQ=255)                             ggAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTAtt                                                                                                                  >  1:320844/1‑151 (MQ=255)                              gAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTAttt                                                                                                                 >  1:719708/1‑151 (MQ=255)                                aTGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtg                                                                                                               >  2:1059636/1‑151 (MQ=255)                                aTGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtg                                                                                                               >  1:544640/1‑151 (MQ=255)                                  gAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtggt                                                                                                             >  1:264147/1‑151 (MQ=255)                                  gAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtggt                                                                                                             >  2:500279/1‑151 (MQ=255)                                   aaaTAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtggtg                                                                                                            >  1:76102/1‑151 (MQ=255)                                   aaaTAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTAGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTtggtg                                                                                                            <  1:1020101/151‑1 (MQ=255)                                      tAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGcc                                                                                                         >  2:558017/1‑151 (MQ=255)                                       aaCTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCa                                                                                                        <  1:155792/151‑1 (MQ=255)                                        aCTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCAt                                                                                                       >  1:605283/1‑151 (MQ=255)                                         cTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCAt                                                                                                       <  1:562397/150‑1 (MQ=255)                                              ttGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTagag                                                                                                  <  1:822351/150‑1 (MQ=255)                                                   cgTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTg                                                                                            <  1:878374/151‑1 (MQ=255)                                                    gTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTg                                                                                            >  2:435566/1‑150 (MQ=255)                                                    gTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTg                                                                                            >  2:808556/1‑150 (MQ=255)                                                       gACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACa                                                                                         <  2:804713/150‑1 (MQ=255)                                                        aCTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGt                                                                                       >  1:353754/1‑151 (MQ=255)                                                        aCTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGt                                                                                       >  2:496369/1‑151 (MQ=255)                                                              aGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTAcg                                                                                 >  1:1266306/1‑151 (MQ=255)                                                                 aaGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTAcgcg                                                                               >  2:167139/1‑150 (MQ=255)                                                                   gCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGaa                                                                             <  2:850/150‑1 (MQ=255)                                                                      aaaGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAAtctg                                                                         <  2:537320/151‑1 (MQ=255)                                                                       aaGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAAtctgt                                                                        <  2:313109/151‑1 (MQ=255)                                                                         gCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGACTGGGAATGCAGTTGCTGTTTAAGCTGCGCCCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGGTTTACGCGAAtctgtc                                                                       >  1:61861/1‑150 (MQ=255)                                                                          ccTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAAtctgtctg                                                                     <  2:785886/151‑1 (MQ=255)                                                                          ccTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAAtctgtct                                                                      <  1:55702/150‑1 (MQ=255)                                                                             ttttCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGa                                                                    <  2:656549/149‑1 (MQ=255)                                                                               ttCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGAcgcg                                                                >  2:786650/1‑151 (MQ=255)                                                                                     ggCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAgg                                                          <  1:56681/151‑1 (MQ=255)                                                                                     ggCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAg                                                           <  1:597775/150‑1 (MQ=255)                                                                                        ttttACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGtt                                                        >  2:680504/1‑150 (MQ=255)                                                                                          ttACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTaa                                                      <  2:49430/150‑1 (MQ=255)                                                                                          ttACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAat                                                     >  1:924489/1‑151 (MQ=255)                                                                                           tACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAat                                                     <  2:446838/150‑1 (MQ=255)                                                                                           tACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAat                                                     <  2:320850/150‑1 (MQ=255)                                                                                             ccaccCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAatat                                                   <  1:107981/150‑1 (MQ=255)                                                                                                ccccTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGtct                                               <  2:361418/151‑1 (MQ=255)                                                                                                    ttCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGATGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCAcc                                           >  2:875119/1‑151 (MQ=255)                                                                                                      cAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCa                                         <  1:205700/151‑1 (MQ=255)                                                                                                        gcgcGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAAc                                       >  1:218000/1‑151 (MQ=255)                                                                                                              ggCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGAtt                                 <  1:1216116/151‑1 (MQ=255)                                                                                                              ggCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGAt                                  <  1:48217/150‑1 (MQ=255)                                                                                                                 gTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGAttttt                              >  2:1000346/1‑151 (MQ=255)                                                                                                                 gTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGAttttt                              <  1:808524/151‑1 (MQ=255)                                                                                                                    tGGGAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTa                             >  1:191200/1‑149 (MQ=255)                                                                                                                     gggAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAg                           <  1:328647/150‑1 (MQ=255)                                                                                                                     gggAATGCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAg                           <  2:1294050/150‑1 (MQ=255)                                                                                                                           gCAGTTGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTa                    <  1:442380/151‑1 (MQ=255)                                                                                                                               ttGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATcc                 >  2:308800/1‑150 (MQ=255)                                                                                                                                tGCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATcc                 >  1:896226/1‑149 (MQ=255)                                                                                                                                 gCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAg               <  2:30181/150‑1 (MQ=255)                                                                                                                                 gCTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAg               <  1:780452/150‑1 (MQ=255)                                                                                                                                  cTGTTTAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGcc             >  1:517317/1‑151 (MQ=255)                                                                                                                                      ttAAGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCcaca          <  2:1243355/150‑1 (MQ=255)                                                                                                                                        aaGCTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGGTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAg       <  1:305661/151‑1 (MQ=255)                                                                                                                                           cTGCGCTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGccc    >  2:1007330/1‑151 (MQ=255)                                                                                                                                             gcgcTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGc  <  2:1074068/151‑1 (MQ=255)                                                                                                                                              cgcTCGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGc  <  2:200107/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAGCGCGTGGCGTCTGGGAATGCAGTTGCTGTTTAAGCTGCGCTAGCAGCGGGTTGTCCTGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGC  >  NC_000913/1348745‑1349035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑