Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,128	C→A	V167V (GTG→GTT)	sapD ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,128	0	C	A	100.0%	263.9 / NA	70	V167V (GTG→GTT)	sapD	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/37);  total (33/37)

GGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAG  >  NC_000913/1352980‑1353275                                                                                                                                                     |                                                                                                                                                    ggCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAAc                                                                                                                                                   >  2:840223/1‑151 (MQ=255)   cTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACtt                                                                                                                                                 >  1:853130/1‑151 (MQ=255)      aTAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCt                                                                                                                                              <  1:485422/151‑1 (MQ=255)        aaGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTg                                                                                                                                             >  2:189653/1‑150 (MQ=255)         aGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGa                                                                                                                                            >  1:780565/1‑150 (MQ=255)           cAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACAt                                                                                                                                         >  2:953114/1‑151 (MQ=255)            aaaaTAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACAtt                                                                                                                                        >  1:930717/1‑151 (MQ=255)             aaaTAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACAtt                                                                                                                                        >  1:268446/1‑150 (MQ=255)             aaaTAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTc                                                                                                                                       <  1:1245323/151‑1 (MQ=255)              aaTAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCa                                                                                                                                      <  2:1072743/151‑1 (MQ=255)                tAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCAcc                                                                                                                                    >  2:123131/1‑151 (MQ=255)                 aGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCAcc                                                                                                                                    <  2:912391/150‑1 (MQ=255)                    ggTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCg                                                                                                                                <  2:18537/151‑1 (MQ=255)                      tATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGt                                                                                                                              >  1:1183353/1‑151 (MQ=255)                      tATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGGTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACGTTCAcc                                                                                                                                    >  1:57076/1‑145 (MQ=255)                         tGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCa                                                                                                                            <  2:258673/150‑1 (MQ=255)                         tGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCa                                                                                                                            <  1:574285/150‑1 (MQ=255)                              ttttGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCAt                                                                                                                      >  2:976295/1‑151 (MQ=255)                                ttagTTGAGACGCGACCGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTc                                                                                                                        >  1:1270007/4‑147 (MQ=255)                                ttGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCAt                                                                                                                      >  1:499801/1‑149 (MQ=255)                                  ggTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCGTCGGTCAACTCATAgg                                                                                                                   <  1:1035655/150‑1 (MQ=255)                                   gTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAggg                                                                                                                  >  1:921233/1‑150 (MQ=255)                                   gTTGAGACGCGTCAGCAGGCCAAAGATTTGCGACTGGGTTGTTGGCTCCATTGAGTTTGTCGGTTCGTCACCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATa                                                                                                                     <  1:46041/147‑1 (MQ=255)                                     tGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGaa                                                                                                                <  1:1039992/150‑1 (MQ=255)                                      gagaCGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGaaa                                                                                                               <  2:628847/150‑1 (MQ=255)                                      gagaCGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGaaa                                                                                                               <  1:446597/150‑1 (MQ=255)                                          cgcgTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTgc                                                                                                          >  1:281572/1‑151 (MQ=255)                                                 gcagGCGAAAGATTTGCGACTGGGTTGTTGGCTCCATTGAGTTGGGCGGTTCGTCAGCAATCAGCCGACGCGGGTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGcgcatcgc                                                                                                   >  1:1021447/1‑151 (MQ=255)                                                  cagGCGAAAGATTTGCGCGTGGGTTGTTGGCTCCAGTGAGTTGGTCGGTGCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCTCCTTCGGTCAACTCATAGGGAAAACTGcgcatcgc                                                                                                   <  1:907064/150‑1 (MQ=255)                                                  cagGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGTTTCGTTAGCAATCAGCAGACGCGGATGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCCGTCAACTCATAGGGAAAACTGcgcgtcg                                                                                                    >  1:16862/1‑149 (MQ=255)                                                      cGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCtt                                                                                              >  1:1149005/1‑151 (MQ=255)                                                       gAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCttt                                                                                             >  1:332039/1‑151 (MQ=255)                                                            aTTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGAt                                                                                        <  2:38743/151‑1 (MQ=255)                                                               tGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCtt                                                                                     >  1:1126885/1‑151 (MQ=255)                                                                gcgcCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCttt                                                                                    <  2:41813/151‑1 (MQ=255)                                                                 cgcCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCttt                                                                                    >  1:965540/1‑150 (MQ=255)                                                                 cgcCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTa                                                                                   <  2:1283545/151‑1 (MQ=255)                                                                   ccTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTaa                                                                                  >  2:967084/1‑150 (MQ=255)                                                                   ccTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAAt                                                                                 <  2:1271087/151‑1 (MQ=255)                                                                         ttgttgGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAc                                                                           >  2:261393/1‑151 (MQ=255)                                                                          tgttgGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcg                                                                          >  1:202994/1‑151 (MQ=255)                                                                           gttgGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcgc                                                                         <  1:258957/151‑1 (MQ=255)                                                                             tgGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCAcgcg                                                                        <  1:127508/150‑1 (MQ=255)                                                                              ggCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCgg                                                                       <  2:803449/150‑1 (MQ=255)                                                                              ggCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCgg                                                                       <  1:495190/150‑1 (MQ=255)                                                                                  ccATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgca                                                                   <  1:316083/150‑1 (MQ=255)                                                                                  ccATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTgc                                                                    <  2:731728/149‑1 (MQ=255)                                                                                       gAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATTCCCACGCGGTGCAGCAGtt                                                             <  2:945243/151‑1 (MQ=255)                                                                                            ggTCGGTTCGTCAGCAATCAGCAGACGCGGGTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATg                                                        <  2:746824/151‑1 (MQ=255)                                                                                                 gTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACg                                                   <  2:891718/151‑1 (MQ=255)                                                                                                  ttCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACgg                                                  >  2:40788/1‑151 (MQ=255)                                                                                                        aGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTg                                            <  2:1095386/151‑1 (MQ=255)                                                                                                        aGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTg                                            >  1:1197481/1‑151 (MQ=255)                                                                                                               agcagACGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCgccag                                      <  1:367563/150‑1 (MQ=255)                                                                                                                 cagACGCGGTTGATTCGCCAGTGCAATGGCGATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCgccagcca                                   <  2:731425/151‑1 (MQ=255)                                                                                                                    aCGCGGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCaa                                  <  1:192451/149‑1 (MQ=255)                                                                                                                     cgcgGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAAc                               <  2:482714/151‑1 (MQ=255)                                                                                                                      gcgGTTGATTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACg                              <  2:853209/151‑1 (MQ=255)                                                                                                                             aTTCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGccac                       >  1:781331/1‑151 (MQ=255)                                                                                                                               tCGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGccacc                      >  1:512458/1‑150 (MQ=255)                                                                                                                                cGCCAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGccacca                     <  2:136520/150‑1 (MQ=255)                                                                                                                                  ccAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACg                  <  1:84336/151‑1 (MQ=255)                                                                                                                                   cAGTGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACg                  >  1:1103016/1‑150 (MQ=255)                                                                                                                                      tGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCt              >  2:1256394/1‑151 (MQ=255)                                                                                                                                      tGCAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCt              >  1:300571/1‑151 (MQ=255)                                                                                                                                        cAATGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCttt            >  1:615043/1‑151 (MQ=255)                                                                                                                                         aaTGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCGGCCAAAACGCTGCCACCAACGGCCt              <  1:948111/148‑1 (MQ=255)                                                                                                                                         aaTGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCttt            >  1:1013026/1‑150 (MQ=255)                                                                                                                                         aaTGGCTATCATAACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTta           <  2:22756/151‑1 (MQ=255)                                                                                                                                             gCTATCATAACTTTCTGACATTCGCCTGCGGTCAACTCGTAGGGAAAACTGCGCATCACATCTTTGTGACCTTTAATCCCCACGCGGTGCAGCGGTTCAATGGCACGGCGTTTGCGCCAGCCAAACCGCTGCCACCAACGGCCt              <  1:131238/144‑1 (MQ=255)                                                                                                                                                  cataaCTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTc     >  2:1163224/1‑148 (MQ=255)                                                                                                                                                  cataaCTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAg  <  2:671532/151‑1 (MQ=255)                                                                                                                                                  cataaCTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAg  <  1:976236/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGGTCGGTTCGTCAGCAATCAGCAGACGCGGTTGATTCGCCAGTGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAG  >  NC_000913/1352980‑1353275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑