Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,367,147 T→G K256Q (AAA→CAA)  pspF ← psp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,367,1470TG97.3% 274.3 / ‑1.2 73K256Q (AAA→CAA) pspFpsp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (31/40);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCGG  >  NC_000913/1367000‑1367294
                                                                                                                                                   |                                                                                                                                                   
cgcgcTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGaa                                                                                                                                                   >  1:416705/1‑150 (MQ=255)
     ttCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGa                                                                                                                                              >  2:959806/1‑150 (MQ=255)
      tCTGGTTATATTTCCCTTGTTGCAAACTTAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGa                                                                                                                                              <  2:717396/149‑1 (MQ=255)
       cTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTTTTCAGGCGGACGCCGTTGAAAGGGATc                                                                                                                                            >  1:681506/1‑150 (MQ=255)
       cTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATc                                                                                                                                            >  1:186880/1‑150 (MQ=255)
           ttATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCaata                                                                                                                                        >  1:871958/1‑150 (MQ=255)
              tatTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAatg                                                                                                                                     >  1:136883/1‑150 (MQ=255)
               atTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAatgat                                                                                                                                   <  2:416471/151‑1 (MQ=255)
               atTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAatga                                                                                                                                    <  2:426733/150‑1 (MQ=255)
                 ttCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGt                                                                                                                                 >  2:771879/1‑151 (MQ=255)
                   cccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGtc                                                                                                                                >  2:788197/1‑150 (MQ=255)
                   cccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGtc                                                                                                                                <  2:839066/150‑1 (MQ=255)
                       tgttgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGtcatca                                                                                                                            <  2:242641/150‑1 (MQ=255)
                         ttgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGc                                                                                                                         >  2:213660/1‑151 (MQ=255)
                          tgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGc                                                                                                                         <  1:675128/150‑1 (MQ=255)
                          tgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGc                                                                                                                         <  2:841179/150‑1 (MQ=255)
                             aaaCTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGa                                                                                                                      >  1:217417/1‑150 (MQ=255)
                               aCTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATaa                                                                                                                   <  1:132513/151‑1 (MQ=255)
                                cTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAACTCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAAt                                                                                                                  >  1:950443/1‑151 (MQ=255)
                                  gAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAAt                                                                                                                  <  2:325747/149‑1 (MQ=255)
                                      tGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTg                                                                                                             <  2:14706/150‑1 (MQ=255)
                                      tGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTg                                                                                                             <  2:589809/150‑1 (MQ=255)
                                        cagcaACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTg                                                                                                          <  1:714871/151‑1 (MQ=255)
                                         agcaACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGc                                                                                                         <  2:1198077/151‑1 (MQ=255)
                                            aaCTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCg                                                                                                       >  1:1102980/1‑150 (MQ=255)
                                             aCTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTg                                                                                                     <  2:103115/151‑1 (MQ=255)
                                              ctctTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTgg                                                                                                    >  1:1048388/1‑151 (MQ=255)
                                                ctTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCg                                                                                                  >  2:48824/1‑151 (MQ=255)
                                                 tttttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCg                                                                                                  >  2:450043/1‑150 (MQ=255)
                                                 tttttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCg                                                                                                  >  1:1014235/1‑150 (MQ=255)
                                                   tttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGAt                                                                                                <  2:132914/150‑1 (MQ=255)
                                                    ttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATac                                                                                              >  1:218959/1‑151 (MQ=255)
                                                    ttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATa                                                                                               <  1:934177/150‑1 (MQ=255)
                                                      ccTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATacac                                                                                            >  1:443155/1‑151 (MQ=255)
                                                            gcATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACg                                                                                      <  2:824577/151‑1 (MQ=255)
                                                               tCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTc                                                                                   >  1:1264342/1‑151 (MQ=255)
                                                                cTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTcc                                                                                  >  1:701313/1‑151 (MQ=255)
                                                                  gAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGGGATACACTGAACGtt                                                                                    <  1:701079/147‑1 (MQ=255)
                                                                  gAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGtt                                                                                    <  1:140931/147‑1 (MQ=255)
                                                                    aaCTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTccacc                                                                               >  2:624400/1‑150 (MQ=255)
                                                                             aaaTCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTc                                                                      <  1:230891/150‑1 (MQ=255)
                                                                                 ccAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTc                                                                 <  2:395740/151‑1 (MQ=255)
                                                                                    gCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACg                                                              <  2:655441/151‑1 (MQ=255)
                                                                                          gtgtTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATAtt                                                        <  2:1008161/151‑1 (MQ=255)
                                                                                            gtTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTc                                                      >  1:629198/1‑151 (MQ=255)
                                                                                              tGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTcc                                                     <  1:226189/150‑1 (MQ=255)
                                                                                                gAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCgg                                                  <  2:803144/151‑1 (MQ=255)
                                                                                                 aaGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGc                                                 >  1:1263459/1‑151 (MQ=255)
                                                                                                    cGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCa                                               >  2:109949/1‑150 (MQ=255)
                                                                                                     gAGGTGGTTTCTGAAACGGCGATAGCGTCTGCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAAc                                             <  2:737259/151‑1 (MQ=255)
                                                                                                          ggtTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATa                                         <  2:103784/150‑1 (MQ=255)
                                                                                                              tCTGAAACGGCGCTAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGGTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTc                                     >  1:876535/1‑150 (MQ=255)
                                                                                                                tGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGc                                  >  1:22497/1‑151 (MQ=255)
                                                                                                                 gAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGTATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCa                                    <  2:1238396/148‑1 (MQ=255)
                                                                                                                 gAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCa                                 <  1:241316/151‑1 (MQ=255)
                                                                                                                   aaCGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAAt                               <  2:483667/151‑1 (MQ=255)
                                                                                                                    aCGGCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTTGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACTATAATTCAGCa                                 <  2:1111109/148‑1 (MQ=255)
                                                                                                                      ggCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGtt                            <  1:415809/151‑1 (MQ=255)
                                                                                                                      ggCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGtt                            <  1:442321/151‑1 (MQ=255)
                                                                                                                       gCGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGttt                           <  2:928435/151‑1 (MQ=255)
                                                                                                                        cGATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTtc                          <  2:15528/151‑1 (MQ=255)
                                                                                                                         gATAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTtc                          <  1:1047719/150‑1 (MQ=255)
                                                                                                                          aTAGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTtct                         <  2:1110605/150‑1 (MQ=255)
                                                                                                                            aGCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTctct                       <  1:959748/150‑1 (MQ=255)
                                                                                                                             gCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTgg                     >  2:1033630/1‑151 (MQ=255)
                                                                                                                             gCGTCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTg                      >  1:638585/1‑150 (MQ=255)
                                                                                                                                tCTTCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGTTACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGgcgc                  >  1:985074/1‑151 (MQ=255)
                                                                                                                                  ttCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGgcgcgc                >  1:1083790/1‑151 (MQ=255)
                                                                                                                                   tCAGGCGGACGCCGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGgcgc                  <  2:1038065/148‑1 (MQ=255)
                                                                                                                                         ggACGCCGTTGAAAGGGATCAATAAGGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTa          <  2:255638/150‑1 (MQ=255)
                                                                                                                                               cGTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGCTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCg   >  2:1132128/1‑151 (MQ=255)
                                                                                                                                                gTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCgg  >  1:1153697/1‑151 (MQ=255)
                                                                                                                                                gTTGAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCgg  <  2:541906/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCGG  >  NC_000913/1367000‑1367294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑