Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,435	A→C	I128L (ATT→CTT)	paaH →	3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,435	0	A	C	100.0%	206.6 / NA	53	I128L (ATT→CTT)	paaH	3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/28);  total (25/28)

CACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTT  >  NC_000913/1459291‑1459579                                                                                                                                                  |                                                                                                                                                 cACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTtgctg                                                                                                                                            >  2:301528/1‑151 (MQ=255)  aCGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGCCCACTAACACTTCGTCAATCTCTATAACCGCGCTtgctg                                                                                                                                            >  1:774950/1‑150 (MQ=255)      gcgggcggcggggccccTGTTAATGGAGGCGGCGTCTGACCGTCGGAAAGAAAAAAACG‑CGCTTTTTGCACGGCGGGCGGATGTTGGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGga                                                                                                                                        <  2:295039/135‑1 (MQ=255)        tGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCgg                                                                                                                                         >  2:543291/1‑147 (MQ=255)               tGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAAt                                                                                                                              >  1:1270150/1‑151 (MQ=255)                    ccttGGTAATTGACGCGGCTTCTGAAC‑TTGGAAAAAAAAAAAAGCCCCTCTTTCCCGGGCTGGGGGATTTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTg                                                                                                                          <  1:1087111/147‑1 (MQ=255)                      tgggTAATGGAAGCGGCGTCTGACGGTGTGAAAAAAAAAAACC‑CGCTTTTCGCACGGCGGGGGAAATTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTg                                                                                                                          <  1:378281/146‑1 (MQ=255)                       tGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACg                                                                                                                      >  2:1198694/1‑151 (MQ=255)                          tCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACgtgt                                                                                                                   >  1:188153/1‑151 (MQ=255)                            aTTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTg                                                                                                                 >  1:943754/1‑151 (MQ=255)                               gAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCgg                                                                                                              >  2:435164/1‑151 (MQ=255)                                agggggcgTCGGACCGTCGGAAAGAAAAAAAAG‑CGTTCTTCGCCCAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCgg                                                                                                              <  2:1176609/146‑1 (MQ=255)                                  ggggcgTCACAACGTGTGAAAGTAAAAAAAG‑CCCTTTTGCCACGGGGGGGGAATTTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGc                                                                                                                <  2:98868/144‑1 (MQ=255)                                             aCGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCAttttttt                                                                                                >  1:417606/1‑151 (MQ=255)                                               gTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTa                                                                                               >  1:268438/1‑150 (MQ=255)                                                     aagaaaaaaagG‑CGCTTTTCGCACGGGGGGGGGAAGTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCg                                                                                          <  2:416312/144‑1 (MQ=255)                                                     aaaaaaaaaaaG‑CTCTTTTTGCACGGCGGGGGGATTTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTa                                                                                               <  2:686021/139‑1 (MQ=255)                                                      aggcAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTAGCGGGGCTGCATTTTTTTAACCCGGc                                                                                        >  1:749081/4‑150 (MQ=255)                                                            aaacG‑CTCTCTTGGCACAGCGGGGGGATGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGtg                                                                                 <  1:415636/151‑1 (MQ=255)                                                                    ctctTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGt                                                                          >  2:822927/1‑151 (MQ=255)                                                                     tttttGCACGGGGGGGGGATTTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGaa                                                                            <  2:1198614/146‑1 (MQ=255)                                                                             caGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAgg                                                                 >  1:568298/1‑151 (MQ=255)                                                                               gCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggtg                                                               >  2:306778/1‑151 (MQ=255)                                                                               gCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggtg                                                               >  1:1100128/1‑151 (MQ=255)                                                                                  ggCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTc                                                            >  2:264245/1‑151 (MQ=255)                                                                                     ggAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAg                                                          >  2:628177/1‑150 (MQ=255)                                                                                      gAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTg                                                        >  2:1004408/1‑151 (MQ=255)                                                                                       aaGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTgg                                                       >  1:1094255/1‑151 (MQ=255)                                                                                        attatGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCa                                                           <  2:1047966/142‑1 (MQ=255)                                                                                          tttCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAgg                                                                 <  2:666278/138‑1 (MQ=255)                                                                                          ttcccccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTggg                                                      <  1:1200720/145‑1 (MQ=255)                                                                                           ttccccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCt                                                    <  2:759380/147‑1 (MQ=255)                                                                                            tGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTgg                                                  >  1:1165064/1‑151 (MQ=255)                                                                                              cccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGc                                                 >  2:810223/1‑150 (MQ=255)                                                                                              cccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCa                                                <  1:597203/151‑1 (MQ=255)                                                                                               ccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTg                                                   <  2:1022894/147‑1 (MQ=255)                                                                                                 gCCACAAACGCTATTGACCACTAACACTTCGTCAATCACTATAGCCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcg                                             <  1:235142/151‑1 (MQ=255)                                                                                                   cacaAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggc                                           >  1:1129763/1‑151 (MQ=255)                                                                                                   cacaAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggc                                           <  2:822401/151‑1 (MQ=255)                                                                                                      aaaCGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggcggc                                        >  2:44295/1‑151 (MQ=255)                                                                                                       aaCGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggcggcg                                       <  2:1104512/151‑1 (MQ=255)                                                                                                        aCGCTATTGACCACTAACACTTCGTCAATCTCAATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggcggcgg                                      >  2:386576/1‑151 (MQ=255)                                                                                                               tGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAgttg                                <  2:521386/150‑1 (MQ=255)                                                                                                                 aCCACTAACACTTCGTCAATCTCTATAACCGCGCTCGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGTTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGttgttg                             <  1:853282/151‑1 (MQ=255)                                                                                                                  ccACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGa                            <  2:218484/151‑1 (MQ=255)                                                                                                                     cTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGc                          <  2:1185799/150‑1 (MQ=255)                                                                                                                       aaCACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCa                         <  1:1198621/149‑1 (MQ=255)                                                                                                                         cacTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTtg                     <  2:171332/151‑1 (MQ=255)                                                                                                                          acTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTtgt                    <  1:316472/151‑1 (MQ=255)                                                                                                                               gTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGaa               <  2:892125/151‑1 (MQ=255)                                                                                                                                 cAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACt             <  1:166799/151‑1 (MQ=255)                                                                                                                                      tctATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGt        >  1:806251/1‑151 (MQ=255)                                                                                                                                            aCCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGtt  <  2:1270227/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 CACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTT  >  NC_000913/1459291‑1459579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑