Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,526,968 T→G L10R (CTC→CGC)  yncH → IPR020099 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,526,9680TG98.9% 357.0 / ‑4.1 94L10R (CTC→CGC) yncHIPR020099 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (50/43);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

TACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTA  >  NC_000913/1526821‑1527114
                                                                                                                                                   |                                                                                                                                                  
tACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCt                                                                                                                                                 >  1:131003/1‑151 (MQ=255)
 aCCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCtt                                                                                                                                                >  1:866879/1‑151 (MQ=255)
 aCCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCt                                                                                                                                                 <  2:56967/150‑1 (MQ=255)
    gAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTttc                                                                                                                                             <  2:553917/151‑1 (MQ=255)
     aaaTTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTttca                                                                                                                                            <  1:795527/151‑1 (MQ=255)
       aTTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTttcatt                                                                                                                                          >  1:291006/1‑151 (MQ=255)
         tACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTttcattc                                                                                                                                         <  1:554039/150‑1 (MQ=255)
          aCAAGAAGTATTAAAAAGGAATGGAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTttcattc                                                                                                                                         >  2:772638/1‑149 (MQ=255)
                  tATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCt                                                                                                                                >  1:338914/1‑150 (MQ=255)
                   aTTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTat                                                                                                                              >  1:381647/1‑151 (MQ=255)
                   aTTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTa                                                                                                                               <  1:815057/150‑1 (MQ=255)
                    ttAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatt                                                                                                                             >  2:594914/1‑151 (MQ=255)
                    ttAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatt                                                                                                                             >  1:287068/1‑151 (MQ=255)
                    ttAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatt                                                                                                                             >  1:1191085/1‑151 (MQ=255)
                     tAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTattt                                                                                                                            >  1:107668/1‑151 (MQ=255)
                      aaaaaGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatttc                                                                                                                           <  1:976671/151‑1 (MQ=255)
                      aaaaaGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatttc                                                                                                                           <  2:486603/151‑1 (MQ=255)
                       aaaaGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatttc                                                                                                                           >  2:1267195/1‑150 (MQ=255)
                        aaaGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatttcat                                                                                                                         <  1:273084/151‑1 (MQ=255)
                          aGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTatttcattt                                                                                                                       <  2:572924/151‑1 (MQ=255)
                             aaTGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCct                                                                                                                    <  2:284436/151‑1 (MQ=255)
                             aaTGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCct                                                                                                                    <  1:249720/151‑1 (MQ=255)
                                gAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGGGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCctct                                                                                                                  <  1:769759/150‑1 (MQ=255)
                                         tGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAaac                                                                                                        >  1:869250/1‑151 (MQ=255)
                                            gTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAaacta                                                                                                      >  2:115623/1‑150 (MQ=255)
                                                         agaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGt                                                                                        >  2:1180711/1‑151 (MQ=255)
                                                          gaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTCCATTCAGCGTGTCTATTTCATTTCCACTGAAAAGAAACTAACTATTcaca                                                                                            <  1:951954/146‑1 (MQ=255)
                                                           aTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGc                                                                                      <  1:352911/151‑1 (MQ=255)
                                                             tATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGc                                                                                      >  2:195057/1‑149 (MQ=255)
                                                              aTGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGa                                                                                   <  2:743938/151‑1 (MQ=255)
                                                               tGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTg                                                                                       >  2:463000/1‑146 (MQ=255)
                                                               tGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGc                                                                                      >  1:1186915/1‑147 (MQ=255)
                                                                gTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGAt                                                                                  <  1:697488/150‑1 (MQ=255)
                                                                 ttatttatTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGt                                                                                >  2:1181384/1‑151 (MQ=255)
                                                                 ttatttatTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGt                                                                                >  1:593603/1‑151 (MQ=255)
                                                                   atttattATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGttt                                                                              <  1:114549/151‑1 (MQ=255)
                                                                   atttattATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGttt                                                                              >  2:1193323/1‑151 (MQ=255)
                                                                       attattCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgt                                                                          >  1:18813/1‑151 (MQ=255)
                                                                         tattCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgtt                                                                         <  2:83587/150‑1 (MQ=255)
                                                                           ttCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgttgtt                                                                      >  2:1185089/1‑151 (MQ=255)
                                                                            tCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgttgtt                                                                      >  2:680154/1‑150 (MQ=255)
                                                                            tCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgttgtt                                                                      <  1:828335/150‑1 (MQ=255)
                                                                               gTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATca                                                                  <  1:1247797/151‑1 (MQ=255)
                                                                                 aaCTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATcac                                                                 >  1:804733/1‑150 (MQ=255)
                                                                                 aaCTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATcac                                                                 <  1:1075164/150‑1 (MQ=255)
                                                                                     ttATCCACAATGATGGGCGTAATTAATTAACTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGc                                                             >  1:1023959/1‑150 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCa                                                            <  1:14692/150‑1 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCa                                                            <  2:414363/150‑1 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCa                                                            <  1:167632/150‑1 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCa                                                            <  2:658431/150‑1 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCAt                                                           >  1:197819/1‑151 (MQ=255)
                                                                                      tATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCAt                                                           >  1:494588/1‑151 (MQ=255)
                                                                                       aTCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCAt                                                           <  1:765180/150‑1 (MQ=255)
                                                                                         ccACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCt                                                        <  2:500659/151‑1 (MQ=255)
                                                                                         ccACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCt                                                        <  2:1124149/151‑1 (MQ=255)
                                                                                          cacaATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCt                                                        >  1:1246545/1‑150 (MQ=255)
                                                                                                gatgGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAAtct                                                 <  1:707764/151‑1 (MQ=255)
                                                                                                   gggCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCa                                               >  2:454478/1‑150 (MQ=255)
                                                                                                           ttaattaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGtttt                                      >  2:1160509/1‑151 (MQ=255)
                                                                                                            taattaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGtttt                                      <  1:584876/150‑1 (MQ=255)
                                                                                                             aattaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGttttt                                     <  1:710073/150‑1 (MQ=255)
                                                                                                              attaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTa                                    >  1:2844/1‑150 (MQ=255)
                                                                                                              attaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTa                                    >  2:24203/1‑150 (MQ=255)
                                                                                                              attaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTa                                    >  2:191867/1‑150 (MQ=255)
                                                                                                               ttaaATTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAat                                  >  2:662433/1‑151 (MQ=255)
                                                                                                                 aaaTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatga                                <  2:1191158/151‑1 (MQ=255)
                                                                                                                 aaaTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatg                                 >  1:929280/1‑150 (MQ=255)
                                                                                                                 aaaTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatg                                 <  1:594896/150‑1 (MQ=255)
                                                                                                                 aaaTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatg                                 >  1:359001/1‑150 (MQ=255)
                                                                                                                   aTTCATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatgatg                              >  2:27283/1‑151 (MQ=255)
                                                                                                                      cATGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGat                           <  2:49761/151‑1 (MQ=255)
                                                                                                                       aTGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGat                           >  2:168360/1‑150 (MQ=255)
                                                                                                                       aTGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGat                           >  1:1005302/1‑150 (MQ=255)
                                                                                                                       aTGGTATGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGat                           >  1:69039/1‑150 (MQ=255)
                                                                                                                            aTGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCt                      <  2:338921/150‑1 (MQ=255)
                                                                                                                            aTGTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTc                     <  2:567288/151‑1 (MQ=255)
                                                                                                                              gTTTTTTAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCg                    <  1:1222136/150‑1 (MQ=255)
                                                                                                                                tttttAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCgg                   >  2:675021/1‑149 (MQ=255)
                                                                                                                                   ttAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGtt               <  2:287074/150‑1 (MQ=255)
                                                                                                                                   ttAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTg              >  2:2491/1‑151 (MQ=255)
                                                                                                                                    tAATTTATATCACTTGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTATATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTg              >  1:1104610/1‑150 (MQ=255)
                                                                                                                                    tAATTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTgg             >  1:998084/1‑151 (MQ=255)
                                                                                                                                     aaTTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTgg             <  1:1043184/150‑1 (MQ=255)
                                                                                                                                     aaTTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTgg             <  2:381656/150‑1 (MQ=255)
                                                                                                                                     aaTTTATATCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGga            <  1:1233861/151‑1 (MQ=255)
                                                                                                                                      aTTTATATCACCTGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTTAAAAGCAACTTACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCACTGTATAATCTCACAATGTCTTTACTGATGGATATGCTCGGAGTTgg             >  1:16233/1‑149 (MQ=255)
                                                                                                                                         tataTCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATg        >  1:1017470/1‑151 (MQ=255)
                                                                                                                                         tataTCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATg        >  2:1244709/1‑151 (MQ=255)
                                                                                                                                          atatCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGc       <  1:424992/151‑1 (MQ=255)
                                                                                                                                          atatCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATg        <  2:410476/150‑1 (MQ=255)
                                                                                                                                          atatCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGc       >  2:522542/1‑151 (MQ=255)
                                                                                                                                          atatCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGc       >  1:1094355/1‑151 (MQ=255)
                                                                                                                                            atCACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTACTGATGGATATGCTCGGAGTTGGAGATGCa      >  2:733747/1‑150 (MQ=255)
                                                                                                                                              cACTCGCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAatt   <  1:273470/151‑1 (MQ=255)
                                                                                                                                                ctcgCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAatta  >  1:862922/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTA  >  NC_000913/1526821‑1527114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑