Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,736,425 T→G N289T (AAT→ACT)  ydhP ← putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,4250TG97.3% 256.2 / ‑2.0 75N289T (AAT→ACT) ydhPputative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (40/33);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

ATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑TTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCACCAGCATTGC  >  NC_000913/1736277‑1736570
                                                                                                                                                     |                                                                                                                                                 
aTTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTg                                                                                                                                                 >  2:1141809/1‑151 (MQ=255)
  ttCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCCCACCACCATGCTAATAGCTGCGCCGAAATCA‑GTg                                                                                                                                                 >  2:1241090/1‑149 (MQ=255)
   tCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCCCACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCgg                                                                                                                                              >  1:906258/1‑151 (MQ=255)
     cAAGATTAAAGGCACCAATAATGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCggg                                                                                                                                             <  2:60506/150‑1 (MQ=255)
       aGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCa                                                                                                                                          <  1:210185/151‑1 (MQ=255)
         aTTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAgg                                                                                                                                        <  1:871199/151‑1 (MQ=255)
          ttAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAgg                                                                                                                                        >  2:95964/1‑150 (MQ=255)
           tAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTTCCGCCCCCCACACCACCATGCCAATAGGCGCGCCGAACTCA‑GTGCGGGCCAGGa                                                                                                                                       >  2:295958/1‑150 (MQ=255)
              aGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGGTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACgg                                                                                                                                   >  2:87298/1‑151 (MQ=255)
                 cACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGAt                                                                                                                                <  2:866963/151‑1 (MQ=255)
                   ccAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑TTGCGGGCCAGGAACGGGAtt                                                                                                                               >  2:764963/1‑150 (MQ=255)
                        atTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGc                                                                                                                         <  1:999543/151‑1 (MQ=255)
                             cTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATaa                                                                                                                     >  2:602387/1‑150 (MQ=255)
                              tgatgaAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGATGCTGCGCCCCCCACCACCATGCTTATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGGTTGCCAGCATaa                                                                                                                     >  2:1209323/1‑149 (MQ=255)
                               gatgaAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATc                                                                                                                   <  1:581550/150‑1 (MQ=255)
                                atgaAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCAc                                                                                                                 >  2:287315/1‑151 (MQ=255)
                                    agacagacCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGGTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GGGCGGGCCAGGAACGGGATTGCCAGCATAATCACCAt                                                                                                              >  1:497840/1‑150 (MQ=255)
                                         gacCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATcaaca                                                                                                         >  2:1117478/1‑150 (MQ=255)
                                           ccTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATcaacaaca                                                                                                      <  2:1052138/151‑1 (MQ=255)
                                           ccTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATcaacaac                                                                                                       <  1:83931/150‑1 (MQ=255)
                                              ggCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATcaacaacaa                                                                                                     <  1:1247470/149‑1 (MQ=255)
                                               gcgcTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAAGCTGCCGCCCCCCACACCACCATGCCAATAGCTGGGCCGAACTCA‑GAGCGGGCCAGGAACGGGATTGCCCGCATAAACACCCTcaacaacaaca                                                                                                   >  1:664326/1‑150 (MQ=255)
                                                 gcTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCCGGAACGGGGTTGCCAGCATAATCACCATCaacaaaaacaaa                                                                                                 >  1:1140711/1‑150 (MQ=255)
                                                 gcTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGAACGCAAAGGTTGCTGCGCCCCAAACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACaaa                                                                                                 >  2:700003/1‑150 (MQ=255)
                                                     cACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGcc                                                                                            <  1:231298/151‑1 (MQ=255)
                                                       cTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCtt                                                                                          >  2:159927/1‑151 (MQ=255)
                                                         ggCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTc                                                                                        <  2:344241/151‑1 (MQ=255)
                                                           cGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTc                                                                                        >  1:398790/1‑149 (MQ=255)
                                                            gACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAAc                                                                                     <  1:1075070/151‑1 (MQ=255)
                                                             acacGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACg                                                                                    <  2:235873/151‑1 (MQ=255)
                                                               acGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTg                                                                                  <  2:789653/151‑1 (MQ=255)
                                                                  cATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGGTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGcc                                                                                >  1:203501/1‑150 (MQ=255)
                                                                    tCACGCGCATCTGTAACGGCGGTACGACCGCCAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGtt                                                                             >  2:940983/1‑151 (MQ=255)
                                                                     cACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTa                                                                            <  2:1140519/151‑1 (MQ=255)
                                                                      aCGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTaa                                                                           <  1:668657/151‑1 (MQ=255)
                                                                         cgcATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACt                                                                         <  1:1261984/150‑1 (MQ=255)
                                                                         cgcATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTg                                                                        <  2:1241285/151‑1 (MQ=255)
                                                                            aTCTGTAACGGCGGTAAGGCCGCAAAGGTTGTTCCCCCACCCAACCCCACCCGAATTTGTCCACCGAACGCA‑GCGCCGGCCCGGAACGGGAGTGCCCGCATTATCGCCCTCAACAACAACAAAAAGCCTTTCAACGGGCCGGTAAACGAAc                                                                     >  1:773762/1‑151 (MQ=255)
                                                                               tGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACg                                                                    >  2:920640/1‑149 (MQ=255)
                                                                                gTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATc                                                                 <  1:102551/151‑1 (MQ=255)
                                                                                 tAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCt                                                                >  1:542371/1‑151 (MQ=255)
                                                                                 tAACGGCGGTACCACCGCAAAGGTTGCTTCGCCCCACACCACCATGCTAATAGCCGCGCCGAACTCAGTGGCGGGCC‑GGAACGGGATTGCCAGCATCATCACCCACAACAAAAAAAAAAAGCCTTTCAAAGGGCCGGTCAATGAACGATc                                                                 >  1:102039/1‑150 (MQ=255)
                                                                                  aaCGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCt                                                                <  2:561622/150‑1 (MQ=255)
                                                                                    cggcggTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGcc                                                             >  1:416157/1‑151 (MQ=255)
                                                                                     ggcggTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGTACTCA‑GTGCGGGCCAGGCACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGcc                                                             >  2:1286705/1‑150 (MQ=255)
                                                                                     ggcggTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGcc                                                             >  1:1070988/1‑150 (MQ=255)
                                                                                     ggcggTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCa                                                            <  2:1200309/151‑1 (MQ=255)
                                                                                     ggcggTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTATCAACGTGCCGTTAACTGAACGATCTGcc                                                             >  2:718481/1‑150 (MQ=255)
                                                                                          tACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTg                                                       >  1:50630/1‑151 (MQ=255)
                                                                                           aCGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTgc                                                      >  1:1152760/1‑151 (MQ=255)
                                                                                           aCGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTgc                                                      >  2:807460/1‑151 (MQ=255)
                                                                                              aCCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTgccgc                                                   >  2:1077427/1‑151 (MQ=255)
                                                                                                 gCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGccgccg                                                 >  2:176685/1‑150 (MQ=255)
                                                                                                       gTTGCTGTGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGt                                          >  2:1222233/1‑151 (MQ=255)
                                                                                                       gTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGt                                          <  1:875135/151‑1 (MQ=255)
                                                                                                         tgctgcGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGtt                                         <  1:770509/150‑1 (MQ=255)
                                                                                                           ctgcGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTAcc                                      >  1:607345/1‑151 (MQ=255)
                                                                                                              cgcCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGAt                                   <  2:567905/151‑1 (MQ=255)
                                                                                                                     caccacCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAAt                            <  2:877085/151‑1 (MQ=255)
                                                                                                                     caccacCATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAAt                            >  2:1270510/1‑151 (MQ=255)
                                                                                                                      accaccATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATc                           <  2:498309/151‑1 (MQ=255)
                                                                                                                      accaccATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATc                           >  1:321825/1‑151 (MQ=255)
                                                                                                                      accaccATGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAAATGAACGAGCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATc                           >  2:696949/1‑151 (MQ=255)
                                                                                                                            aTGCTAATAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGacac                     <  2:416167/151‑1 (MQ=255)
                                                                                                                                 aaTAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCaa                  <  1:338037/149‑1 (MQ=255)
                                                                                                                                 aaTAGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATc                >  2:602501/1‑151 (MQ=255)
                                                                                                                                    aGCTGCGCCGAACTCA‑GTGCGGGCCAGGAATGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGACTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTATCGATAGAGAATCCGACACCAATCa               >  1:115696/1‑149 (MQ=255)
                                                                                                                                    aGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGc             <  2:622052/151‑1 (MQ=255)
                                                                                                                                    aGCTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGc             <  1:377048/151‑1 (MQ=255)
                                                                                                                                      cTGCGCCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCAc           >  2:1246661/1‑151 (MQ=255)
                                                                                                                                        gcgcCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCAcc          <  2:287070/150‑1 (MQ=255)
                                                                                                                                         cgcCGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCAcc          >  1:531237/1‑149 (MQ=255)
                                                                                                                                           ccGAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCACCAGc       <  1:36830/150‑1 (MQ=255)
                                                                                                                                             gAACTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCACCAGCAtt    <  1:423104/151‑1 (MQ=255)
                                                                                                                                               aCTCA‑GTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCACCAGCATTGc  >  1:370714/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACACCACCATGCTAATAGCTGCGCCGAACTCA‑TTGCGGGCCAGGAACGGGATTGCCAGCATAATCACCATCAACAACAACAAAAAGCCTTTCAACGTGCCGTTAACTGAACGATCTGCCAGTTTGCCGCCGAGATAGTTACCGATAGAGAATCCGACACCAATCAGCACCAGCATTGC  >  NC_000913/1736277‑1736570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑