Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,052	T→G	K183T (AAA→ACA)	zapD ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,052	0	T	G	100.0%	388.8 / NA	97	K183T (AAA→ACA)	zapD	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (54/43);  total (54/43)

CAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATC  >  NC_000913/111906‑112197                                                                                                                                                   |                                                                                                                                                  cAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACg                                                                                                                                               <  2:367691/151‑1 (MQ=255)  aGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACgg                                                                                                                                              >  1:99070/1‑151 (MQ=255)  aGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACgg                                                                                                                                              >  2:812956/1‑151 (MQ=255)    tgtCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGa                                                                                                                                             >  2:263941/1‑150 (MQ=255)     gtCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAACTTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATACAAACCATTCAGGCTGGTTTGTGTACGGa                                                                                                                                             >  2:738396/1‑149 (MQ=255)     gtCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGaa                                                                                                                                            <  2:182806/150‑1 (MQ=255)       ccAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAggg                                                                                                                                         >  1:382974/1‑151 (MQ=255)        cAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAgggg                                                                                                                                        >  2:1091705/1‑151 (MQ=255)        cAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAgggg                                                                                                                                        <  2:650169/151‑1 (MQ=255)         aGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAggggg                                                                                                                                       >  2:1255603/1‑151 (MQ=255)         aGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAggggg                                                                                                                                       >  2:460212/1‑151 (MQ=255)              cATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGAc                                                                                                                                  <  1:711678/151‑1 (MQ=255)              cATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGAc                                                                                                                                  >  2:137436/1‑151 (MQ=255)               aTAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGAc                                                                                                                                  >  1:771150/1‑150 (MQ=255)               aTAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACt                                                                                                                                 <  2:1087435/151‑1 (MQ=255)                   aaCGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCTAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCg                                                                                                                             >  2:791655/1‑151 (MQ=255)                     cGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGa                                                                                                                            >  1:1067693/1‑150 (MQ=255)                        aTGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAAtt                                                                                                                         >  1:940952/1‑150 (MQ=255)                         tGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTa                                                                                                                        >  1:961917/1‑150 (MQ=255)                                aCGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGc                                                                                                                <  2:951632/151‑1 (MQ=255)                                 cGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGc                                                                                                                >  2:344760/1‑150 (MQ=255)                                        tATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATg                                                                                                          <  2:1265147/149‑1 (MQ=255)                                         aTGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGt                                                                                                        >  1:34781/1‑150 (MQ=255)                                         aTGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTa                                                                                                       <  2:363720/151‑1 (MQ=255)                                           gTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTaa                                                                                                      <  2:760918/150‑1 (MQ=255)                                           gTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTa                                                                                                       >  2:396085/1‑149 (MQ=255)                                            tCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGt                                                                                                    >  1:114118/1‑151 (MQ=255)                                              cGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGc                                                                                                  >  1:1298715/1‑151 (MQ=255)                                                gAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCt                                                                                                >  2:278349/1‑151 (MQ=255)                                                 aaaTTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTg                                                                                               >  1:226656/1‑151 (MQ=255)                                                  aaTTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTgg                                                                                              >  1:1241899/1‑151 (MQ=255)                                                     ttGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATAAAATCCAGCACCATGGTAAGTGCCTGGGTg                                                                                           >  2:722228/1‑151 (MQ=255)                                                        cGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAg                                                                                         >  1:224127/1‑150 (MQ=255)                                                        cGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAg                                                                                         >  2:80964/1‑150 (MQ=255)                                                        cGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGc                                                                                        <  1:1218101/151‑1 (MQ=255)                                                           aTAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCggg                                                                                     >  1:157973/1‑151 (MQ=255)                                                               aGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCa                                                                                 <  2:821620/151‑1 (MQ=255)                                                               aGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCa                                                                                 <  1:1149034/151‑1 (MQ=255)                                                               aGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCa                                                                                 <  2:36168/151‑1 (MQ=255)                                                                gCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTc                                                                                  >  1:172407/1‑149 (MQ=255)                                                                gCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAg                                                                                <  1:610584/151‑1 (MQ=255)                                                                 cTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAgg                                                                               >  1:224434/1‑151 (MQ=255)                                                                     gAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTgg                                                                           >  2:113636/1‑151 (MQ=255)                                                                      aaTCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGc                                                                          >  1:564649/1‑151 (MQ=255)                                                                      aaTCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGc                                                                          >  2:1294942/1‑151 (MQ=255)                                                                       aTCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGc                                                                          >  2:372690/1‑150 (MQ=255)                                                                       aTCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCa                                                                         <  1:98934/151‑1 (MQ=255)                                                                        tCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAGGTGCCTGGGTGAGCGGGTTCAGGCTGGCa                                                                         >  2:703738/1‑150 (MQ=255)                                                                        tCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCa                                                                         >  1:804593/1‑150 (MQ=255)                                                                          gAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATc                                                                      >  1:158378/1‑151 (MQ=255)                                                                            gCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCa                                                                    <  2:496715/151‑1 (MQ=255)                                                                              gACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAgg                                                                  <  2:573317/151‑1 (MQ=255)                                                                               aCAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGt                                                                 <  2:820887/151‑1 (MQ=255)                                                                                cAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCATCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGtt                                                                >  2:947945/1‑151 (MQ=255)                                                                                cAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGtt                                                                >  2:1039326/1‑151 (MQ=255)                                                                                  gATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTc                                                              <  1:1182980/151‑1 (MQ=255)                                                                                  gATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTc                                                              <  1:156164/151‑1 (MQ=255)                                                                                     tCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTa                                                            <  1:1066511/150‑1 (MQ=255)                                                                                      cAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTAcc                                                          >  1:707133/1‑151 (MQ=255)                                                                                      cAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGAACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTAcc                                                          <  2:1240900/151‑1 (MQ=255)                                                                                       aGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCt                                                         >  1:1057538/1‑151 (MQ=255)                                                                                       aGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCt                                                         <  1:514075/151‑1 (MQ=255)                                                                                           gcagcaAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGc                                                      <  2:1160470/150‑1 (MQ=255)                                                                                            cagcaAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTg                                                    >  1:318191/1‑151 (MQ=255)                                                                                            cagcaAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTg                                                    <  2:59258/151‑1 (MQ=255)                                                                                             agcaAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGt                                                   <  1:1030287/151‑1 (MQ=255)                                                                                                 aGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTcgcg                                               >  1:50811/1‑151 (MQ=255)                                                                                                          cGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTg                                      <  2:992/151‑1 (MQ=255)                                                                                                          cGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTg                                      >  1:949620/1‑151 (MQ=255)                                                                                                            ccACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTggg                                    >  1:619379/1‑151 (MQ=255)                                                                                                             cACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTgggg                                   <  1:928226/151‑1 (MQ=255)                                                                                                              aCCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGt                                  <  1:1082912/151‑1 (MQ=255)                                                                                                                cGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAg                                <  2:1269526/151‑1 (MQ=255)                                                                                                                   tATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGAt                              <  1:1236606/150‑1 (MQ=255)                                                                                                                   tATCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATg                             >  2:1059028/1‑151 (MQ=255)                                                                                                                     tCCTGATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGAGAGCGGGTTCAGGCTGGTAATCCAGGTATCTACCTGGCTGACGCGAAGAGCCGGGGGAAGa                               >  1:692951/1‑147 (MQ=255)                                                                                                                         gATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCa                       <  2:1245605/151‑1 (MQ=255)                                                                                                                         gATAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCa                       <  1:25942/151‑1 (MQ=255)                                                                                                                          aTAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCaa                      <  1:1228574/151‑1 (MQ=255)                                                                                                                          aTAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCa                       >  2:536128/1‑150 (MQ=255)                                                                                                                           tAAAAACCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCaa                      >  1:662191/1‑150 (MQ=255)                                                                                                                            aaaaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAt                    <  2:1128810/151‑1 (MQ=255)                                                                                                                            aaaaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAt                    <  2:236291/151‑1 (MQ=255)                                                                                                                             aaaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAtg                   >  2:591198/1‑151 (MQ=255)                                                                                                                             aaaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAtg                   <  1:941884/151‑1 (MQ=255)                                                                                                                             aaaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGCTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAtg                   >  1:151273/1‑151 (MQ=255)                                                                                                                              aaaCCATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAAtg                   >  1:126872/1‑150 (MQ=255)                                                                                                                                 ccATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGc                <  1:940560/150‑1 (MQ=255)                                                                                                                                  cATTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCaa              <  1:318857/151‑1 (MQ=255)                                                                                                                                   aTTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAAt             <  2:690768/151‑1 (MQ=255)                                                                                                                                   aTTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAAt             >  2:1045328/1‑151 (MQ=255)                                                                                                                                   aTTCAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAAt             >  2:271120/1‑151 (MQ=255)                                                                                                                                      cAGGCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTa          <  1:249017/151‑1 (MQ=255)                                                                                                                                         gCTGGTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGt       >  2:236162/1‑151 (MQ=255)                                                                                                                                            ggTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTaa     <  1:377589/150‑1 (MQ=255)                                                                                                                                            ggTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTaa     <  1:169056/150‑1 (MQ=255)                                                                                                                                            ggTTTGTGTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTaa     >  1:653889/1‑150 (MQ=255)                                                                                                                                             gtttgtgtACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAAt   >  2:1069680/1‑151 (MQ=255)                                                                                                                                               ttgtgtACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATc  >  1:377666/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATC  >  NC_000913/111906‑112197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑