Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,924,700 T→G intergenic (‑587/+35) yqcC ← / ← syd DUF446 family protein/SecY‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,924,7000TG97.1% 399.5 / ‑0.8 105intergenic (‑587/+35)yqcC/sydDUF446 family protein/SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (63/39);  total (65/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 105 total aligned reads displayed.

TACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTACACAC  >  NC_000913/2924555‑2924845
                                                                                                                                                 |                                                                                                                                                 
tACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAag                                                                                                                                                >  1:582019/1‑149 (MQ=255)
   aaGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAag                                                                                                                                                >  1:92655/1‑146 (MQ=255)
   aaGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAag                                                                                                                                                >  1:1184875/1‑146 (MQ=255)
             cAGACAATGCCTTAAGGAAAATCTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAAcc                                                                                                                                 <  2:1019090/151‑1 (MQ=255)
               gACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAAcc                                                                                                                                 >  2:302210/1‑149 (MQ=255)
                 cAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGAt                                                                                                                             <  1:47407/151‑1 (MQ=255)
                   aTGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATgg                                                                                                                           <  1:1242094/151‑1 (MQ=255)
                   aTGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATgg                                                                                                                           >  1:53178/1‑151 (MQ=255)
                     gCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATgggg                                                                                                                         <  2:946758/151‑1 (MQ=255)
                      ccTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        <  1:1137510/151‑1 (MQ=255)
                      ccTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        >  1:1151286/1‑151 (MQ=255)
                       cTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        >  2:953931/1‑150 (MQ=255)
                        ttAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAt                                                                                                                      >  2:1281245/1‑151 (MQ=255)
                        ttAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAt                                                                                                                      >  2:727483/1‑151 (MQ=255)
                         tAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAtt                                                                                                                     >  1:1005667/1‑151 (MQ=255)
                         tAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAt                                                                                                                      <  2:1281202/150‑1 (MQ=255)
                          aaGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAttt                                                                                                                    >  2:1190396/1‑151 (MQ=255)
                          aaGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAttt                                                                                                                    <  1:336993/151‑1 (MQ=255)
                           aGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTg                                                                                                                   >  2:798101/1‑151 (MQ=255)
                           aGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTg                                                                                                                   <  1:520284/151‑1 (MQ=255)
                           aGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTg                                                                                                                   <  1:327420/151‑1 (MQ=255)
                             gAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTgg                                                                                                                  >  2:560439/1‑150 (MQ=255)
                                  tttCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                                                                                                             >  1:114193/1‑150 (MQ=255)
                                     cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagaa                                                                                                         <  2:649923/151‑1 (MQ=255)
                                     cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagaa                                                                                                         <  2:307693/151‑1 (MQ=255)
                                     cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagaa                                                                                                         <  2:969146/151‑1 (MQ=255)
                                     cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                                                                                                          >  1:1187617/1‑150 (MQ=255)
                                     cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                                                                                                          >  2:413971/1‑150 (MQ=255)
                                      atatGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                                                                                                          >  2:1290662/1‑149 (MQ=255)
                                        atGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTTTTTGGATTACAGAagag                                                                                                      <  1:281708/151‑1 (MQ=255)
                                        atGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagaaga                                                                                                       <  2:284890/150‑1 (MQ=255)
                                          gAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAgg                                                                                                     <  1:420561/150‑1 (MQ=255)
                                          gAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGc                                                                                                    >  1:98036/1‑151 (MQ=255)
                                           aaaGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCt                                                                                                   >  1:289493/1‑151 (MQ=255)
                                              gTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTaa                                                                                                <  1:685275/151‑1 (MQ=255)
                                               ttAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTa                                                                                                 >  1:940782/1‑149 (MQ=255)
                                               ttAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTa                                                                                                 >  2:1239769/1‑149 (MQ=255)
                                                tAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGt                                                                                              >  2:965158/1‑151 (MQ=255)
                                                 aaGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGtt                                                                                             <  1:226586/151‑1 (MQ=255)
                                                 aaGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGt                                                                                              <  1:677965/150‑1 (MQ=255)
                                                 aaGAATTAACAATCAACAGTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGtt                                                                                             <  2:1280604/151‑1 (MQ=255)
                                                   gAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTg                                                                                            >  2:1136005/1‑150 (MQ=255)
                                                   gAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGa                                                                                           <  2:435509/151‑1 (MQ=255)
                                                    aaTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGAt                                                                                          >  1:1039627/1‑151 (MQ=255)
                                                     aTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGAtt                                                                                         <  1:600876/151‑1 (MQ=255)
                                                        aaCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTa                                                                                        >  1:370130/1‑149 (MQ=255)
                                                         aCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAgg                                                                                     >  1:153723/1‑151 (MQ=255)
                                                           aaTCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGaa                                                                                   <  2:538659/151‑1 (MQ=255)
                                                            aTCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAAt                                                                                  >  1:613104/1‑151 (MQ=255)
                                                              cAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTc                                                                                >  2:54793/1‑151 (MQ=255)
                                                               aaCATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTcc                                                                               >  1:487527/1‑151 (MQ=255)
                                                                aCATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCg                                                                              <  1:836818/151‑1 (MQ=255)
                                                                aCATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCg                                                                              <  2:530858/151‑1 (MQ=255)
                                                                  aTTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCa                                                                            <  1:315088/151‑1 (MQ=255)
                                                                     ttGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCa                                                                            >  1:991865/1‑148 (MQ=255)
                                                                       gATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAtt                                                                       >  1:736201/1‑151 (MQ=255)
                                                                       gATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAtt                                                                       <  2:1023971/151‑1 (MQ=255)
                                                                       gATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAtt                                                                       >  2:146733/1‑151 (MQ=255)
                                                                        aTTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAttt                                                                      >  2:73362/1‑151 (MQ=255)
                                                                        aTTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAttt                                                                      <  1:467666/151‑1 (MQ=255)
                                                                         ttttAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTg                                                                     >  2:106936/1‑151 (MQ=255)
                                                                           ttAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTg                                                                     >  2:488874/1‑149 (MQ=255)
                                                                            tAAATGAAAATAACGTTTCTTATCTTTTTAAGTATTCGCTTATTTCTTGGCGATGTCCTAAAGAGTCTGTGAGAGATCTCTCACAACCAGTTGGGGTATTTGTATACCAGAATTGACTTTAGTTGATTAAGGAATTCCGAAAGATTTGa                                                                    >  1:1126485/1‑149 (MQ=255)
                                                                            tAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAgg                                                                  >  2:83781/1‑151 (MQ=255)
                                                                            tAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAgg                                                                  >  1:1108352/1‑151 (MQ=255)
                                                                            tAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAgg                                                                  >  1:1296387/1‑151 (MQ=255)
                                                                               aTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTa                                                               <  2:1039691/151‑1 (MQ=255)
                                                                                 gAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTaa                                                              >  2:936723/1‑150 (MQ=255)
                                                                                    aaTAACGATTCTTATCTTTCTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGc                                                          >  1:127329/1‑151 (MQ=255)
                                                                                        aCGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTcc                                                      >  2:156066/1‑151 (MQ=255)
                                                                                            ttCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGttt                                                  >  2:455472/1‑151 (MQ=255)
                                                                                             tCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGttt                                                  <  2:892143/150‑1 (MQ=255)
                                                                                                tATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTAc                                                <  1:15014/149‑1 (MQ=255)
                                                                                                 aTCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACAAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTa                                                 >  1:613449/1‑147 (MQ=255)
                                                                                                  tCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACgtgt                                            >  1:433410/1‑151 (MQ=255)
                                                                                                      tttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTccc                                        >  2:1217210/1‑151 (MQ=255)
                                                                                                       ttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTccc                                        >  1:1121045/1‑150 (MQ=255)
                                                                                                       ttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTccc                                        >  2:516027/1‑150 (MQ=255)
                                                                                                           gTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGt                                   >  2:681483/1‑151 (MQ=255)
                                                                                                                cGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCtt                              >  2:267028/1‑151 (MQ=255)
                                                                                                                 gCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCttt                             <  2:21148/151‑1 (MQ=255)
                                                                                                                  cTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTg                            >  1:1073736/1‑151 (MQ=255)
                                                                                                                    tATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTg                            <  2:209193/149‑1 (MQ=255)
                                                                                                                    tATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGc                           <  1:277161/150‑1 (MQ=255)
                                                                                                                       ttCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGcaca                        <  1:1138689/150‑1 (MQ=255)
                                                                                                                        tCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACt                      >  1:186870/1‑151 (MQ=255)
                                                                                                                         ccTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACtt                     >  2:171050/1‑151 (MQ=255)
                                                                                                                          cTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTc                    >  1:1281385/1‑151 (MQ=255)
                                                                                                                           tGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTc                    >  2:1201228/1‑150 (MQ=255)
                                                                                                                             gCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTc                    <  2:226515/148‑1 (MQ=255)
                                                                                                                             gCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCAcc                 >  1:855557/1‑151 (MQ=255)
                                                                                                                                 tctcCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCgg               >  1:201807/1‑149 (MQ=255)
                                                                                                                                    ccTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGga              <  2:452459/147‑1 (MQ=255)
                                                                                                                                      tACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGAtt        >  2:554256/1‑151 (MQ=255)
                                                                                                                                      tACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGAtt        >  2:1041307/1‑151 (MQ=255)
                                                                                                                                      tACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTATTTGCACACTTCACAGGAGAGAtt        >  1:71999/1‑151 (MQ=255)
                                                                                                                                       aCAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGAtt        <  2:1269701/150‑1 (MQ=255)
                                                                                                                                         agagTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTac      >  1:994011/1‑150 (MQ=255)
                                                                                                                                          gagTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTacac    <  1:520385/151‑1 (MQ=255)
                                                                                                                                            gTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTacacac  >  2:716746/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTACACAC  >  NC_000913/2924555‑2924845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: