Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,902	T→G	D85A (GAT→GCT)	thyA ←	thymidylate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,902	0	T	G	100.0%	350.8 / NA	89	D85A (GAT→GCT)	thyA	thymidylate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/49);  total (40/49)

GTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTA  >  NC_000913/2964754‑2965048                                                                                                                                                     |                                                                                                                                                   gTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCg                                                                                                                                                  <  1:482511/151‑1 (MQ=255)    ccACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGcc                                                                                                                                               >  2:1171218/1‑151 (MQ=255)    ccACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGcc                                                                                                                                               >  1:664404/1‑151 (MQ=255)          tGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCg                                                                                                                                         >  1:129319/1‑151 (MQ=255)           gAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTc                                                                                                                                          <  2:680980/149‑1 (MQ=255)           gAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGt                                                                                                                                        >  2:341299/1‑151 (MQ=255)            aaaCAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTc                                                                                                                                       >  2:538936/1‑151 (MQ=255)                aataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTccc                                                                                                                                     >  2:139299/1‑149 (MQ=255)                aataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCa                                                                                                                                    <  1:806973/150‑1 (MQ=255)                aataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAg                                                                                                                                   <  2:764910/151‑1 (MQ=255)                   aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATg                                                                                                                                <  1:709653/151‑1 (MQ=255)                      gcggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGt                                                                                                                              <  2:861830/150‑1 (MQ=255)                      gcggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTg                                                                                                                             <  1:875086/151‑1 (MQ=255)                         gcgcGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACa                                                                                                                          <  2:869997/151‑1 (MQ=255)                          cgcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACAt                                                                                                                         <  1:1253990/151‑1 (MQ=255)                           gcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACAtt                                                                                                                        <  2:148558/151‑1 (MQ=255)                           gcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACAtt                                                                                                                        <  1:1247090/151‑1 (MQ=255)                           gagactCCGGGTCGTTTTTCCGCTGGCTAAGTACCGTAGTGATCTGGTCAATATGACGGCCATCTGGGGTTGACCAGCCGCGCCACTGTTTACCGTACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGCTGGCGACa                                                                                                                          >  1:487838/6‑149 (MQ=255)                            cgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTg                                                                                                                       <  1:414966/151‑1 (MQ=255)                            cgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTg                                                                                                                       <  1:99456/151‑1 (MQ=255)                             gAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGt                                                                                                                      >  2:1144237/1‑151 (MQ=255)                               aTCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTGAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGttt                                                                                                                    <  2:425723/151‑1 (MQ=255)                               aTCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGttt                                                                                                                    >  2:348256/1‑151 (MQ=255)                                tCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGtttt                                                                                                                   >  1:1201544/1‑151 (MQ=255)                                  cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                                                                                                                 >  1:833330/1‑151 (MQ=255)                                  cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                                                                                                                 >  2:746237/1‑151 (MQ=255)                                     gTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtgt                                                                                                              >  2:905018/1‑151 (MQ=255)                                        gTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCACGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtgt                                                                                                              >  1:588955/1‑148 (MQ=255)                                         tttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtgt                                                                                                              >  2:664780/1‑147 (MQ=255)                                          ttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGAt                                                                                                          <  1:819209/150‑1 (MQ=255)                                           tttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         <  2:754910/150‑1 (MQ=255)                                           tttCAGCTGGTTCAGGACCGTAGTGATCTGGTCAATATGACGACCATCTGGCTTTGGCCAGGCGCGCCACTTTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         <  1:762084/150‑1 (MQ=255)                                             tCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAg                                                                                                       <  2:427965/150‑1 (MQ=255)                                             tCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAg                                                                                                       <  2:395557/150‑1 (MQ=255)                                             tCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAg                                                                                                       <  2:626754/150‑1 (MQ=255)                                              cAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAg                                                                                                       <  1:191676/149‑1 (MQ=255)                                              cAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGc                                                                                                      >  1:357887/1‑150 (MQ=255)                                                   ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGt                                                                                                 <  1:940035/150‑1 (MQ=255)                                                    gTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTa                                                                                               <  1:834637/151‑1 (MQ=255)                                                       cAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgt                                                                                             <  2:74854/150‑1 (MQ=255)                                                        aGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgtgt                                                                                           >  1:1079444/1‑151 (MQ=255)                                                        aGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgtgt                                                                                           <  1:26756/151‑1 (MQ=255)                                                         gTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTc                                                                                          <  1:482968/151‑1 (MQ=255)                                                          tACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCg                                                                                         >  1:728890/1‑151 (MQ=255)                                                            ccGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGcc                                                                                       <  1:1274018/151‑1 (MQ=255)                                                            ccGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGcc                                                                                       >  1:778350/1‑151 (MQ=255)                                                             cGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGcc                                                                                       >  1:680387/1‑150 (MQ=255)                                                                aGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGc                                                                                   >  2:392717/1‑151 (MQ=255)                                                                 gTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGc                                                                                   >  2:302826/1‑150 (MQ=255)                                                                   gATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGc                                                                                   >  2:956442/1‑148 (MQ=255)                                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGaaa                                                                              <  1:563281/148‑1 (MQ=255)                                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAAcc                                                                            <  1:507661/150‑1 (MQ=255)                                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAAcc                                                                            <  1:762550/150‑1 (MQ=255)                                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACca                                                                           <  2:898009/151‑1 (MQ=255)                                                                           cAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACcac                                                                          >  2:1140867/1‑149 (MQ=255)                                                                                 gacgacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGtt                                                                   >  1:899308/1‑150 (MQ=255)                                                                                 gacgacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGtt                                                                   <  1:486403/150‑1 (MQ=255)                                                                                   cgacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCAt                                                                <  2:537542/151‑1 (MQ=255)                                                                                       cATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgat                                                            <  1:1264050/151‑1 (MQ=255)                                                                                        aTCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgatg                                                           >  2:30782/1‑151 (MQ=255)                                                                                           tGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGatgatg                                                        >  1:1096764/1‑151 (MQ=255)                                                                                            ggCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgatga                                                          <  2:347028/148‑1 (MQ=255)                                                                                            ggCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgatg                                                           <  2:915840/147‑1 (MQ=255)                                                                                            ggCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGatgatg                                                        <  1:101365/150‑1 (MQ=255)                                                                                            ggCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGatgatg                                                        <  2:852656/150‑1 (MQ=255)                                                                                                     cAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAgg                                               >  1:919236/1‑150 (MQ=255)                                                                                                      aGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTg                                             <  2:1014688/151‑1 (MQ=255)                                                                                                            gcCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACg                                       >  1:696615/1‑151 (MQ=255)                                                                                                             ccACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGt                                      <  1:252817/151‑1 (MQ=255)                                                                                                                cTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGttt                                    >  1:1140474/1‑150 (MQ=255)                                                                                                                 tGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGttt                                    >  1:1289810/1‑149 (MQ=255)                                                                                                                  gTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGt                                 >  2:807232/1‑151 (MQ=255)                                                                                                                   tttACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGCGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGCGGCAACGTTTAGtt                                >  2:57324/1‑151 (MQ=255)                                                                                                                     tACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGt                              >  2:409922/1‑151 (MQ=255)                                                                                                                       ccATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCa                            <  1:242418/151‑1 (MQ=255)                                                                                                                         aTACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCAcc                          >  1:920555/1‑151 (MQ=255)                                                                                                                          tACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGAGGCAACGTTTAGTTGTCAcc                          >  2:1037056/1‑150 (MQ=255)                                                                                                                           acacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCAcc                          >  2:56261/1‑149 (MQ=255)                                                                                                                           acacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCAcc                          >  2:1117420/1‑149 (MQ=255)                                                                                                                           acacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAg                        <  1:1166877/151‑1 (MQ=255)                                                                                                                            cacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCa                            >  2:832622/1‑146 (MQ=255)                                                                                                                            cacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGc                       <  2:690083/151‑1 (MQ=255)                                                                                                                             acTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCg                      <  2:1262812/151‑1 (MQ=255)                                                                                                                                ggCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCggg                    <  1:152085/150‑1 (MQ=255)                                                                                                                                          cGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTg         <  2:1124559/151‑1 (MQ=255)                                                                                                                                           gCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGc        <  1:583118/151‑1 (MQ=255)                                                                                                                                           gCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGc        <  2:275116/151‑1 (MQ=255)                                                                                                                                             cGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAg      <  2:1262235/151‑1 (MQ=255)                                                                                                                                                  tCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTa  >  2:369071/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTA  >  NC_000913/2964754‑2965048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑