Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,035,568 A→C D213E (GAT→GAG)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,035,5680AC98.8% 285.9 / ‑3.8 80D213E (GAT→GAG) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (34/45);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

TGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCG  >  NC_000913/3035420‑3035715
                                                                                                                                                    |                                                                                                                                                   
tGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTc                                                                                                                                                   >  1:643437/1‑151 (MQ=255)
 ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTcc                                                                                                                                                  >  2:889526/1‑151 (MQ=255)
 ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTcc                                                                                                                                                  <  2:1061114/151‑1 (MQ=255)
 ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTcc                                                                                                                                                  >  2:396916/1‑151 (MQ=255)
  gCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTccg                                                                                                                                                 >  2:661729/1‑151 (MQ=255)
     tGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTccgccg                                                                                                                                              >  2:1088598/1‑151 (MQ=255)
      gATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTccgcc                                                                                                                                               <  1:186019/149‑1 (MQ=255)
        tCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCggg                                                                                                                                            >  1:731783/1‑150 (MQ=255)
        tCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGt                                                                                                                                           >  1:191917/1‑151 (MQ=255)
           ttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGTTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTg                                                                                                                                         <  1:1197415/150‑1 (MQ=255)
           ttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGtt                                                                                                                                          <  2:298277/149‑1 (MQ=255)
            tGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAt                                                                                                                                       >  2:825771/1‑151 (MQ=255)
             gTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAtt                                                                                                                                      >  2:575955/1‑151 (MQ=255)
             gTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAtt                                                                                                                                      <  1:976298/151‑1 (MQ=255)
              ttCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAttt                                                                                                                                     >  1:1093939/1‑151 (MQ=255)
                cTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCg                                                                                                                                   >  2:1301356/1‑151 (MQ=255)
                  tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGCCCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTc                                                                                                                                    <  2:1064532/148‑1 (MQ=255)
                  tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGa                                                                                                                                  <  2:379772/150‑1 (MQ=255)
                   gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTTCGATAAACGTCCATGCGCAGCTCCGCCGGGTTGATTTCGat                                                                                                                                 >  2:737032/1‑150 (MQ=255)
                   gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGat                                                                                                                                 >  1:644853/1‑150 (MQ=255)
                    tgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatat                                                                                                                               >  1:149013/1‑151 (MQ=255)
                     gCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatc                                                                                                                              >  2:1269052/1‑151 (MQ=255)
                         ggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaata                                                                                                                          <  2:892014/151‑1 (MQ=255)
                         ggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaata                                                                                                                          <  2:1033557/151‑1 (MQ=255)
                          gAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatat                                                                                                                         <  2:465341/151‑1 (MQ=255)
                             cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcat                                                                                                                      <  2:619066/151‑1 (MQ=255)
                             cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcat                                                                                                                      <  1:1282652/151‑1 (MQ=255)
                              ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatc                                                                                                                     >  2:234457/1‑151 (MQ=255)
                               gTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatc                                                                                                                     >  1:199400/1‑150 (MQ=255)
                                 cGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatcatc                                                                                                                  <  2:790132/151‑1 (MQ=255)
                                 cGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatcatc                                                                                                                  <  2:940272/151‑1 (MQ=255)
                                 cGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatcat                                                                                                                   <  2:840542/150‑1 (MQ=255)
                                  gTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatcatc                                                                                                                  <  2:714548/150‑1 (MQ=255)
                                        gCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTcc                                                                                                           <  2:758360/151‑1 (MQ=255)
                                          aCTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCgg                                                                                                         >  2:821899/1‑151 (MQ=255)
                                          aCTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCgg                                                                                                         <  2:878751/151‑1 (MQ=255)
                                              ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaa                                                                                                     <  1:786441/151‑1 (MQ=255)
                                                 cACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaaca                                                                                                  <  2:470194/151‑1 (MQ=255)
                                                   cGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaa                                                                                                <  1:432353/151‑1 (MQ=255)
                                                    gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac                                                                                               >  2:197712/1‑151 (MQ=255)
                                                     aTCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcg                                                                                              <  2:556766/151‑1 (MQ=255)
                                                       cccGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcgc                                                                                             <  2:740598/150‑1 (MQ=255)
                                                           gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGc                                                                                        <  1:845706/151‑1 (MQ=255)
                                                           gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGc                                                                                        >  1:360211/1‑151 (MQ=255)
                                                                 aTGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg                                                                                  <  1:1000598/151‑1 (MQ=255)
                                                                  tgtgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg                                                                                  >  2:1020369/1‑150 (MQ=255)
                                                                     gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacgacg                                                                               <  1:1139743/150‑1 (MQ=255)
                                                                      ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACgtg                                                                             <  1:213582/151‑1 (MQ=255)
                                                                       gTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACgtgt                                                                            >  1:679315/1‑151 (MQ=255)
                                                                         aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGtgtg                                                                           <  1:1074075/150‑1 (MQ=255)
                                                                         aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGtgtg                                                                           <  1:1091142/150‑1 (MQ=255)
                                                                          aTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACAACGTGTGGc                                                                         <  1:80961/151‑1 (MQ=255)
                                                                               cACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgacg                                                                     >  2:1279986/1‑150 (MQ=255)
                                                                                aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                                                                   >  2:182092/1‑151 (MQ=255)
                                                                                aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                                                                   >  2:243473/1‑151 (MQ=255)
                                                                                   gCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGAccgc                                                                <  1:989572/151‑1 (MQ=255)
                                                                                       aTTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCgg                                                             >  1:778146/1‑150 (MQ=255)
                                                                                          cgggccGGTTAAGGTGCTGCCCGCCCGCGCCGGACGGGCGATAAACGTCAATGCGCAGCGCCGCCGGGTTGATTTCGATAACAAGATCATCATCAACTTCCGGATAAGCAACCGGGGAGCTGAACGACGTGAGGCGCCGACCGCCGGAGTc                                                         <  1:100018/146‑1 (MQ=255)
                                                                                              aCGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCaaa                                                      <  1:205469/150‑1 (MQ=255)
                                                                                                ggTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACgg                                                   <  1:1097186/151‑1 (MQ=255)
                                                                                                    aaCGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCt                                                <  2:835902/150‑1 (MQ=255)
                                                                                                         gCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACg                                          <  2:522136/151‑1 (MQ=255)
                                                                                                            gACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCAc                                       <  1:316188/151‑1 (MQ=255)
                                                                                                              ccgcccgcGCCGGACGTTCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCa                                     <  1:737005/151‑1 (MQ=255)
                                                                                                                  ccgcGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCg                                 <  2:603905/151‑1 (MQ=255)
                                                                                                                      gcCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGa                             <  2:731810/151‑1 (MQ=255)
                                                                                                                      gcCGGACGAGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATCTCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTg                              >  2:47673/1‑150 (MQ=255)
                                                                                                                        cGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAAc                           <  2:298002/151‑1 (MQ=255)
                                                                                                                               gCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGt                     <  2:1150513/150‑1 (MQ=255)
                                                                                                                                 gATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTc                  >  2:721300/1‑151 (MQ=255)
                                                                                                                                 gATAAACGTCAAGGCGCAGCTCCGCCGTGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGttt                   <  1:1154241/150‑1 (MQ=255)
                                                                                                                                       cGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTAc             >  2:1097256/1‑150 (MQ=255)
                                                                                                                                       cGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACg            >  2:1189635/1‑151 (MQ=255)
                                                                                                                                        gTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCGCCAGGCGGTGAACGCCGGTTTCTGTACGc           <  1:734060/151‑1 (MQ=255)
                                                                                                                                             tGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGcagcc       >  2:339481/1‑150 (MQ=255)
                                                                                                                                              gcgcAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGcagcca      <  1:945404/150‑1 (MQ=255)
                                                                                                                                               cgcAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGcagccagc    >  1:935883/1‑151 (MQ=255)
                                                                                                                                               cgcAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGcagccag     <  1:1167353/150‑1 (MQ=255)
                                                                                                                                                gcAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCagccagcc   >  1:433361/1‑151 (MQ=255)
                                                                                                                                                 cAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCg  >  1:765924/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCG  >  NC_000913/3035420‑3035715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑