Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,213,290	C→A	Q82K (CAG→AAG)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,213,290	0	C	A	100.0%	286.0 / NA	73	Q82K (CAG→AAG)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/34);  total (39/34)

AAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGG  >  NC_000913/3213144‑3213434                                                                                                                                                   |                                                                                                                                                 aaGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGt                                                                                                                                              >  2:748809/1‑151 (MQ=255) aaGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGt                                                                                                                                              <  2:528788/151‑1 (MQ=255)  aGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTg                                                                                                                                             >  1:1178053/1‑151 (MQ=255)         gtcgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCa                                                                                                                                      <  2:1220107/151‑1 (MQ=255)          tcgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGTATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTcc                                                                                                                                       >  1:91386/1‑149 (MQ=255)           cgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAg                                                                                                                                     <  1:992872/150‑1 (MQ=255)            gATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCg                                                                                                                                   >  1:1059546/1‑151 (MQ=255)               tcagatcagaTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGTCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTgg                                                                                                                                >  2:606620/1‑151 (MQ=255)                cagatcagatCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGa                                                                                                                               <  2:366309/151‑1 (MQ=255)                   atcagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATc                                                                                                                            <  1:738786/151‑1 (MQ=255)                   atcagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATc                                                                                                                            >  2:144002/1‑151 (MQ=255)                     cagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGTATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCt                                                                                                                           <  1:820313/150‑1 (MQ=255)                     cagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTg                                                                                                                          <  2:398274/151‑1 (MQ=255)                       gatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGa                                                                                                                         >  1:316547/1‑150 (MQ=255)                           gAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCg                                                                                                                    >  1:1256609/1‑151 (MQ=255)                            aaGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCgg                                                                                                                   >  1:1146905/1‑151 (MQ=255)                            aaGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCgg                                                                                                                   >  2:1144858/1‑151 (MQ=255)                            aaGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCgg                                                                                                                   >  1:120475/1‑151 (MQ=255)                             aGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCggg                                                                                                                  <  2:803911/151‑1 (MQ=255)                             aGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCggg                                                                                                                  >  1:68218/1‑151 (MQ=255)                              gACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGgc                                                                                                                 >  2:978252/1‑151 (MQ=255)                               aCATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGgcg                                                                                                                <  2:311284/151‑1 (MQ=255)                                catcatCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGgcgc                                                                                                               >  1:910667/1‑151 (MQ=255)                                catcatCCAAATGATCAACGACATGGGCATGCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGgcgc                                                                                                               <  2:745192/151‑1 (MQ=255)                                   catcCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACg                                                                                                            <  2:1075799/151‑1 (MQ=255)                                       cAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTg                                                                                                        >  1:131137/1‑151 (MQ=255)                                       cAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGAGGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTg                                                                                                        <  1:927876/151‑1 (MQ=255)                                        aaaTGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTg                                                                                                        <  1:683033/150‑1 (MQ=255)                                            gATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGAccc                                                                                                    <  1:545025/150‑1 (MQ=255)                                            gATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCg                                                                                                   <  2:673568/151‑1 (MQ=255)                                              tCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGt                                                                                                 >  2:939558/1‑151 (MQ=255)                                               cAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGt                                                                                                 >  2:767508/1‑150 (MQ=255)                                                aaCGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTa                                                                                                <  2:65349/150‑1 (MQ=255)                                                 aCGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACg                                                                                              <  1:421608/151‑1 (MQ=255)                                                       tGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGt                                                                                         >  2:368565/1‑150 (MQ=255)                                                       tGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGt                                                                                         >  2:7434/1‑150 (MQ=255)                                                        gggCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTa                                                                                        <  1:1242257/150‑1 (MQ=255)                                                        gggCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTAc                                                                                       <  2:1070732/151‑1 (MQ=255)                                                           cATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATg                                                                                    <  1:219142/151‑1 (MQ=255)                                                                aGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTg                                                                                >  2:147368/1‑150 (MQ=255)                                                                   tGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAAt                                                                            >  1:491186/1‑151 (MQ=255)                                                                   tGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAAt                                                                            >  2:206211/1‑151 (MQ=255)                                                                      tGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATggg                                                                         >  2:29101/1‑151 (MQ=255)                                                                         aagaagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAc                                                                      >  2:856322/1‑151 (MQ=255)                                                                                    gATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGt                                                           >  2:1160737/1‑151 (MQ=255)                                                                                     aTGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGtt                                                          >  1:308538/1‑151 (MQ=255)                                                                                             gatCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg                                                  >  2:913651/1‑151 (MQ=255)                                                                                             gatCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc                                                   <  1:510421/150‑1 (MQ=255)                                                                                                cTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                 >  1:462088/1‑149 (MQ=255)                                                                                                 tGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAgg                                              >  1:177747/1‑151 (MQ=255)                                                                                                  gATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGc                                             <  2:948044/151‑1 (MQ=255)                                                                                                   aTGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCg                                            <  1:312752/151‑1 (MQ=255)                                                                                                     gctggctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGa                                           >  1:863487/1‑150 (MQ=255)                                                                                                        ggctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa                                         <  1:428012/149‑1 (MQ=255)                                                                                                           tgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGAc                                    >  2:613307/1‑151 (MQ=255)                                                                                                             aaaaCACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACAt                                  <  2:801035/151‑1 (MQ=255)                                                                                                               aaCACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCg                                >  1:672772/1‑151 (MQ=255)                                                                                                                acacCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGc                               >  1:906975/1‑151 (MQ=255)                                                                                                                     gcgGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGc                          <  1:136598/151‑1 (MQ=255)                                                                                                                        gACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa                       <  1:39077/151‑1 (MQ=255)                                                                                                                         aCGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAt                      >  2:26323/1‑151 (MQ=255)                                                                                                                          cGAAGATGCTGCCGCAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAtt                     <  1:622572/151‑1 (MQ=255)                                                                                                                           gAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg                    <  1:9567/151‑1 (MQ=255)                                                                                                                           gAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg                    <  1:857776/151‑1 (MQ=255)                                                                                                                              gATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAg                 <  1:144000/151‑1 (MQ=255)                                                                                                                                tgctgcCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGa                <  2:1253654/150‑1 (MQ=255)                                                                                                                                      cGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc         >  1:785359/1‑151 (MQ=255)                                                                                                                                       gAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa        <  1:368557/151‑1 (MQ=255)                                                                                                                                        aaGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa       <  1:499766/151‑1 (MQ=255)                                                                                                                                            cgccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc     >  2:1153588/1‑149 (MQ=255)                                                                                                                                            cgccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc     >  2:12029/1‑149 (MQ=255)                                                                                                                                             gccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAgg  >  2:1039222/1‑151 (MQ=255)                                                                                                                                              ccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAgg  >  1:443038/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGG  >  NC_000913/3213144‑3213434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑