Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,912	A→C	K140T (AAA→ACA)	patA →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,912	0	A	C	100.0%	285.2 / NA	72	K140T (AAA→ACA)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/39);  total (33/39)

GGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGC  >  NC_000913/3219767‑3220053                                                                                                                                                  |                                                                                                                                              ggAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTg                                                                                                                                           >  2:301326/1‑150 (MQ=255) ggAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGa                                                                                                                                          <  2:774774/151‑1 (MQ=255)        tCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATAc                                                                                                                                     >  2:394994/1‑149 (MQ=255)            cGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGcttc                                                                                                                               >  1:298429/1‑151 (MQ=255)                 ggCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTg                                                                                                                          <  1:474001/151‑1 (MQ=255)                     ccGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTaa                                                                                                                       <  2:862622/150‑1 (MQ=255)                     ccGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAAt                                                                                                                      >  2:183587/1‑151 (MQ=255)                     ccGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACACTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAAt                                                                                                                      <  2:1184938/151‑1 (MQ=255)                      cGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATa                                                                                                                     <  2:251609/151‑1 (MQ=255)                         aaTCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCg                                                                                                                  >  2:292360/1‑151 (MQ=255)                          aTCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGc                                                                                                                   >  1:345041/1‑149 (MQ=255)                            ccAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGc                                                                                                                >  1:55023/1‑150 (MQ=255)                              aGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCAcc                                                                                                             >  2:661397/1‑151 (MQ=255)                                 tgtgGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCg                                                                                                            >  2:26377/1‑149 (MQ=255)                                   tgGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTc                                                                                                        >  1:87650/1‑151 (MQ=255)                                    ggTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTcc                                                                                                       >  2:715765/1‑151 (MQ=255)                                    ggTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTc                                                                                                        <  2:743435/150‑1 (MQ=255)                                    ggTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTGCTTCTGTAATAGCGGCACCGAGTcc                                                                                                       <  1:174137/151‑1 (MQ=255)                                          cgccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGaa                                                                                                 <  1:925342/151‑1 (MQ=255)                                          cgccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGa                                                                                                  >  2:510710/1‑150 (MQ=255)                                            ccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAagc                                                                                               <  1:887315/151‑1 (MQ=255)                                             cgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAagca                                                                                              <  2:277315/151‑1 (MQ=255)                                                aCAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAagcagc                                                                                            <  1:1031400/150‑1 (MQ=255)                                                 cAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAgcgc                                                                                          <  2:639427/151‑1 (MQ=255)                                                   gAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTg                                                                                        <  1:1110745/151‑1 (MQ=255)                                                    aaTCAACTTGCTAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGa                                                                                       <  2:733250/151‑1 (MQ=255)                                                    aaTCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTg                                                                                        >  1:1137461/1‑150 (MQ=255)                                                       cAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAg                                                                                     <  2:687687/150‑1 (MQ=255)                                                              cGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGaa                                                                             <  1:782042/151‑1 (MQ=255)                                                              cGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGaa                                                                             <  1:105987/151‑1 (MQ=255)                                                                aaaCAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGaa                                                                             >  2:1255724/1‑149 (MQ=255)                                                                      ccGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTAcc                                                                     <  2:884558/151‑1 (MQ=255)                                                                       cGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCa                                                                    <  2:279995/151‑1 (MQ=255)                                                                          tGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTc                                                                 >  2:684791/1‑151 (MQ=255)                                                                           gCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCa                                                                <  1:1265395/151‑1 (MQ=255)                                                                            cacaGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCAc                                                               >  1:1106310/1‑151 (MQ=255)                                                                              caGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCAcc                                                              >  1:312829/1‑150 (MQ=255)                                                                                   aGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCgg                                                        <  2:623762/151‑1 (MQ=255)                                                                                      agttgctCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACcgcg                                                           <  2:411825/142‑1 (MQ=255)                                                                                      aGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCaa                                                     <  2:637694/151‑1 (MQ=255)                                                                                      aGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCa                                                      >  2:1081737/1‑150 (MQ=255)                                                                                          gctCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGt                                                   <  2:225754/149‑1 (MQ=255)                                                                                             cGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACt                                              <  2:927412/151‑1 (MQ=255)                                                                                              gATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACtt                                             >  2:616309/1‑151 (MQ=255)                                                                                              gATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACtt                                             >  2:1235404/1‑151 (MQ=255)                                                                                              gATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACtt                                             >  1:1015493/1‑151 (MQ=255)                                                                                                 ccGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACtttt                                           <  1:1300012/150‑1 (MQ=255)                                                                                                 ccGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACtttt                                           <  2:739440/150‑1 (MQ=255)                                                                                                  cGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTAt                                         <  1:999009/151‑1 (MQ=255)                                                                                                  cGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTAt                                         <  2:961633/151‑1 (MQ=255)                                                                                                   gTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTAtt                                        <  2:777938/151‑1 (MQ=255)                                                                                                     tACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGc                                      >  1:482186/1‑151 (MQ=255)                                                                                                      aCGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGcc                                     <  1:562473/151‑1 (MQ=255)                                                                                                           cGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAg                                <  2:979540/151‑1 (MQ=255)                                                                                                                 tGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAgcggcg                           >  1:141148/1‑150 (MQ=255)                                                                                                                 tGGCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAgcggcg                           <  2:1220270/150‑1 (MQ=255)                                                                                                                   gCGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGt                        >  1:562254/1‑151 (MQ=255)                                                                                                                    cGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGtt                       >  1:1053626/1‑151 (MQ=255)                                                                                                                    cGAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGtt                       >  1:624538/1‑151 (MQ=255)                                                                                                                     gAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTc                      >  1:389247/1‑151 (MQ=255)                                                                                                                     gAAAACCCTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTc                      >  2:832440/1‑151 (MQ=255)                                                                                                                           ccTTGCTGCGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGt                <  1:581725/151‑1 (MQ=255)                                                                                                                               gctgcGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTaaa             <  2:508912/150‑1 (MQ=255)                                                                                                                                 tgcGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATc           >  2:38746/1‑150 (MQ=255)                                                                                                                                 tgcGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATc           >  2:946064/1‑150 (MQ=255)                                                                                                                                 tgcGCTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATc           >  2:954283/1‑150 (MQ=255)                                                                                                                                  gcgcTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCAc         <  2:539744/151‑1 (MQ=255)                                                                                                                                  gcgcTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCAc         <  1:766874/151‑1 (MQ=255)                                                                                                                                     cTAACGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTg      >  2:988095/1‑151 (MQ=255)                                                                                                                                       aaCGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTg      <  1:661374/149‑1 (MQ=255)                                                                                                                                        aCGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGgcg   <  1:1079174/151‑1 (MQ=255)                                                                                                                                         cGCCCGGTACACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGgcgc  >  1:359190/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                              GGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGC  >  NC_000913/3219767‑3220053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑