Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,239,097	T→G	L173R (CTG→CGG)	alx →	putative membrane‑bound redox modulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,239,097	0	T	G	100.0%	261.6 / NA	78	L173R (CTG→CGG)	alx	putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/36);  total (42/36)

TTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGG  >  NC_000913/3238950‑3239243                                                                                                                                                    |                                                                                                                                                   ttCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCggg                                                                                                                                                  >  2:436228/1‑150 (MQ=255)  tCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCggg                                                                                                                                                  >  2:616758/1‑149 (MQ=255)    tGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTgc                                                                                                                                               >  2:938001/1‑150 (MQ=255)     gCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTgcgc                                                                                                                                             >  1:1232707/1‑151 (MQ=255)        tACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTgg                                                                                                                                          >  2:588806/1‑151 (MQ=255)          ccATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCt                                                                                                                                        <  1:996872/151‑1 (MQ=255)             tcatGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTCCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTa                                                                                                                                       <  1:1018385/149‑1 (MQ=255)                 gATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCgg                                                                                                                                  <  1:616079/150‑1 (MQ=255)                  aTCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCgg                                                                                                                                  <  1:878607/149‑1 (MQ=255)                    cTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCAt                                                                                                                              >  1:357353/1‑151 (MQ=255)                    cTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGAGGTCAt                                                                                                                              >  1:1070778/1‑151 (MQ=255)                    cTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGGGCGCTGGCTACGCGGTCAt                                                                                                                              >  2:522785/1‑151 (MQ=255)                      tCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCAtt                                                                                                                             <  1:163583/150‑1 (MQ=255)                               gCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGa                                                                                                                    >  2:289345/1‑150 (MQ=255)                               gCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGAc                                                                                                                   >  1:389617/1‑151 (MQ=255)                               gCTGGCTAATTTCGCAGCTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGCAGATGGCGCCCCCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGCGCGCTGGCTACGCGGTCATTTgcgc                                                                                                                        >  1:986100/1‑146 (MQ=255)                                 tGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGGGCGCTGGCTACGCGGTCATTTGCGCATGAcc                                                                                                                  >  1:830567/1‑150 (MQ=255)                                    cTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGac                                                                                                               >  2:498839/1‑150 (MQ=255)                                           cGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGTGCTCTCCCATGAAGATGAATCAGGCATTGGGGGGAAGCCGCGGGTGCGCTTGCTACGCGGGGATTTTCGCATGACCGACAcc                                                                                                            >  1:882904/1‑146 (MQ=255)                                             cAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCCGGCCTTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCCTTTGCGCATGACCGACACCATc                                                                                                         >  1:680750/1‑147 (MQ=255)                                             cAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCg                                                                                                        >  1:613730/1‑148 (MQ=255)                                             cAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGAc                                                                                                      >  2:1097764/1‑150 (MQ=255)                                                  cGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACg                                                                                                  >  1:818713/1‑149 (MQ=255)                                                  cGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGa                                                                                                 <  2:958034/150‑1 (MQ=255)                                                  cGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAg                                                                                                <  2:911144/151‑1 (MQ=255)                                                   gACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGc                                                                                               >  2:187196/1‑151 (MQ=255)                                                   gACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGc                                                                                               <  2:77108/151‑1 (MQ=255)                                                     cTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCAt                                                                                             >  2:172680/1‑151 (MQ=255)                                                       ggATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                                                           >  1:644322/1‑151 (MQ=255)                                                       ggATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                                                           <  2:623300/151‑1 (MQ=255)                                                       ggATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGAGCGCTGGCTACGCGGTCATTTGTGCATGACCGAAACCATCGACAACCAGCAttt                                                                                           >  1:1215393/1‑151 (MQ=255)                                                       ggATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                                                           >  2:117026/1‑151 (MQ=255)                                                       ggATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCCCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                                                           >  2:1196398/1‑151 (MQ=255)                                                              gTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCCCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgtg                                                                                    >  2:563234/1‑151 (MQ=255)                                                               tatataTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCCCGCCCATGAAGATGAATAAGGCATTAGCGACAAACCGCGGGTGCGCTGGCCACGGGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgcg                                                                                    >  1:925047/1‑148 (MQ=255)                                                               tataTCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgtg                                                                                    >  1:607216/1‑150 (MQ=255)                                                                atatCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCg                                                                                  >  2:682573/1‑151 (MQ=255)                                                                  atCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGt                                                                                 >  2:332781/1‑150 (MQ=255)                                                                  atCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGt                                                                                 <  2:1105099/150‑1 (MQ=255)                                                                      tCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTaa                                                                               <  1:337583/148‑1 (MQ=255)                                                                      tCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAg                                                                              <  1:964171/149‑1 (MQ=255)                                                                      tCGGTGCCTTCCTGCTGTTTACAGGCGTGAAGCTGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGa                                                                             >  1:920189/1‑150 (MQ=255)                                                                       cGGTGCCTTCCTGCTTTTTCCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAt                                                                           <  1:692689/151‑1 (MQ=255)                                                                        ggTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATg                                                                          <  1:33767/151‑1 (MQ=255)                                                                        ggTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATg                                                                          <  2:1257304/151‑1 (MQ=255)                                                                           gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGa                                                                        <  2:120572/150‑1 (MQ=255)                                                                            ccTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAtt                                                                      <  2:125234/151‑1 (MQ=255)                                                                              ttCCTGCTGTTTACCGGCGTGAAGATTGCGCTCCCCCATGAAGATCGATCAGGCATTGGCGACAAGCCGCGGGTGCGCTCGCGACGAGGGCATTTGCGCATGACCGACACCATCGACAACGAACATTTCTTTTCTCGTAAGAATGGAGTg                                                                     >  1:4579/1‑150 (MQ=255)                                                                               tCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTg                                                                     <  2:40445/149‑1 (MQ=255)                                                                                ccTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCt                                                                   <  2:924630/150‑1 (MQ=255)                                                                                ccTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTg                                                                  <  1:1074885/151‑1 (MQ=255)                                                                                 ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGt                                                                 >  2:867797/1‑151 (MQ=255)                                                                                   gctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGt                                                                 <  1:1242270/149‑1 (MQ=255)                                                                                   gctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTa                                                                <  2:695414/150‑1 (MQ=255)                                                                                     tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGGGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                                                              >  1:1039538/1‑150 (MQ=255)                                                                                     tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAACCGCGGGTGCGCTTGCCACGCGGGCATTTGCGCATGCCCGACACCATCGACAACGAGCATTTCTTTGTGCGGAAGAATGGATTGCTGTACGc                                                             >  1:966689/1‑151 (MQ=255)                                                                                      gTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                                                              <  1:1293698/149‑1 (MQ=255)                                                                                            cGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACAcc                                                       >  1:598243/1‑150 (MQ=255)                                                                                                  gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAt                                                 <  2:1040122/150‑1 (MQ=255)                                                                                                   aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATg                                                <  2:403237/150‑1 (MQ=255)                                                                                                    aGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCt                                              <  1:872636/151‑1 (MQ=255)                                                                                                     gATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTg                                             <  2:964014/151‑1 (MQ=255)                                                                                                     gATGGCGCTCGCACATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGATGGCTACGCGGTCATTTGCGCATGACGGACACCCTCGACAACGAGCATTTCTTTGTGCGTAAGCATGGATTGCTGCACGCCACACCGCTGATGCTg                                             >  1:1178054/1‑151 (MQ=255)                                                                                                       tGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTg                                             <  1:264500/149‑1 (MQ=255)                                                                                                         gcgcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTAc                                         <  1:860516/151‑1 (MQ=255)                                                                                                              cGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGa                                      <  2:515376/149‑1 (MQ=255)                                                                                                              cGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGa                                      <  1:596324/149‑1 (MQ=255)                                                                                                                     gAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCtggtgg                             >  2:441128/1‑151 (MQ=255)                                                                                                                     gAAGATGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCtggtgg                             >  2:401044/1‑151 (MQ=255)                                                                                                                         aTGAATCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAAtt                         >  1:1032228/1‑151 (MQ=255)                                                                                                                           gagtCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTg                        <  2:763362/147‑1 (MQ=255)                                                                                                                             aTCAGGCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGc                     <  2:910398/151‑1 (MQ=255)                                                                                                                                  gCATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGt                >  1:914361/1‑151 (MQ=255)                                                                                                                                   cATTGGCGACAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTg               <  2:524035/151‑1 (MQ=255)                                                                                                                                           aCAAGCCGCGGGTGCGCTGGCTACGCGGTGATATGCGCATGACCGGCTCCATCGACAACGAGCCTGCCTTTGTGCGTAAGAACGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTTTGGTGGAATTGAGCGACGTGATTTTCg        <  1:1034710/150‑1 (MQ=255)                                                                                                                                            cAAGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGcc      >  2:229408/1‑151 (MQ=255)                                                                                                                                              aGCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGt    >  2:901283/1‑151 (MQ=255)                                                                                                                                               gCCGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTg   <  1:299656/151‑1 (MQ=255)                                                                                                                                                ccGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTgg  >  2:1059967/1‑151 (MQ=255)                                                                                                                                                ccGCGGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTgg  >  2:1184846/1‑151 (MQ=255)                                                                                                                                                 cgcgGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTgg  <  2:357361/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTCTGCGTACCATCATGATCTTCACTGGCAGCTGGCTAATTTCGCAGTTCGACTGGATCCTGTATATCTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGG  >  NC_000913/3238950‑3239243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑