Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,247,589 T→G intergenic (+161/‑184) exuR → / → yqjA hexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,247,5890TG99.0% 375.4 / ‑3.6 96intergenic (+161/‑184)exuR/yqjAhexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (34/61);  total (34/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

ACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAA  >  NC_000913/3247441‑3247734
                                                                                                                                                    |                                                                                                                                                 
aCAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGt                                                                                                                                                 <  1:125045/151‑1 (MQ=255)
  aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGtt                                                                                                                                                >  2:171818/1‑150 (MQ=255)
  aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTa                                                                                                                                               <  1:395209/151‑1 (MQ=255)
  aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTa                                                                                                                                               <  2:213118/151‑1 (MQ=255)
     cgcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACaa                                                                                                                                            <  2:717100/151‑1 (MQ=255)
     cgcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACa                                                                                                                                             >  1:166536/1‑150 (MQ=255)
      gcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAAt                                                                                                                                           <  1:579985/151‑1 (MQ=255)
         gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAAtt                                                                                                                                          <  1:109769/149‑1 (MQ=255)
         gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTa                                                                                                                                         <  1:1218777/150‑1 (MQ=255)
         gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTa                                                                                                                                         <  1:522568/150‑1 (MQ=255)
         gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAg                                                                                                                                        <  1:976077/151‑1 (MQ=255)
           aaTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGa                                                                                                                                       <  2:1041240/150‑1 (MQ=255)
           aaTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGAt                                                                                                                                      <  2:297872/151‑1 (MQ=255)
              gcgcCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCa                                                                                                                                   <  1:1263645/151‑1 (MQ=255)
               cgcCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCaa                                                                                                                                  <  1:497930/151‑1 (MQ=255)
                gcCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAAt                                                                                                                                 >  1:1107521/1‑151 (MQ=255)
                gcCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAAt                                                                                                                                 <  2:55760/151‑1 (MQ=255)
                   tGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTg                                                                                                                              >  2:203397/1‑151 (MQ=255)
                    gTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAAttt                                                                                                                               <  1:698141/149‑1 (MQ=255)
                    gTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAAttt                                                                                                                               <  1:474905/149‑1 (MQ=255)
                    gTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTg                                                                                                                              <  2:107227/150‑1 (MQ=255)
                    gTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTg                                                                                                                              >  2:1080324/1‑150 (MQ=255)
                    gTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTg                                                                                                                              <  1:500120/150‑1 (MQ=255)
                       tGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAAt                                                                                                                           <  2:325686/150‑1 (MQ=255)
                            cgtcgGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGt                                                                                                                     <  1:39808/151‑1 (MQ=255)
                             gtcgGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGCCTCCACGGACATGTAATTTTGATAACGAAAAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTa                                                                                                                        <  2:701775/147‑1 (MQ=255)
                               cgGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGttt                                                                                                                   <  2:457990/150‑1 (MQ=255)
                                    aGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAAt                                                                                                             <  1:1288718/151‑1 (MQ=255)
                                     gCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATg                                                                                                            <  2:405177/151‑1 (MQ=255)
                                       gAAAAGTATAAAGTGTCAGCCTGTGTAAATCCCCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCt                                                                                                          <  1:396180/151‑1 (MQ=255)
                                         aaaGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCttt                                                                                                        <  2:122861/151‑1 (MQ=255)
                                           aGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCt                                                                                                      >  2:1265077/1‑151 (MQ=255)
                                                aaaGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTAtc                                                                                                  <  2:555376/150‑1 (MQ=255)
                                                aaaGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTAtc                                                                                                  <  2:71286/150‑1 (MQ=255)
                                                aaaGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTAtc                                                                                                  <  2:381252/150‑1 (MQ=255)
                                                    tgtCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACg                                                                                             >  1:606560/1‑151 (MQ=255)
                                                     gtCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGa                                                                                            <  2:446701/151‑1 (MQ=255)
                                                       cAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGAtt                                                                                          >  2:327829/1‑151 (MQ=255)
                                                        aGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGAttt                                                                                         <  1:820867/151‑1 (MQ=255)
                                                        aGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGAttt                                                                                         >  2:436887/1‑151 (MQ=255)
                                                         gCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGAttt                                                                                         >  1:1024826/1‑150 (MQ=255)
                                                          ccTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTaa                                                                                       <  1:255164/151‑1 (MQ=255)
                                                            tgtgTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAAc                                                                                      >  1:41494/1‑150 (MQ=255)
                                                             gtgtAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAAc                                                                                      >  2:677327/1‑149 (MQ=255)
                                                             gtgtAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAg                                                                                    <  2:821339/151‑1 (MQ=255)
                                                               gtAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAggg                                                                                  >  2:784033/1‑151 (MQ=255)
                                                                   aTCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGCTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAAt                                                                               >  1:169042/1‑150 (MQ=255)
                                                                        ctcGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTc                                                                          >  1:1136070/1‑150 (MQ=255)
                                                                             cACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGTATTTATGTTTTTGAATGCTTTCTTATCACACGATTTAACAGGGAATAGTTCAGGCt                                                                     <  2:451745/150‑1 (MQ=255)
                                                                             cACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCtg                                                                    >  1:908517/1‑151 (MQ=255)
                                                                              aCCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCtgt                                                                   >  2:1266601/1‑151 (MQ=255)
                                                                               cccTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCtgtg                                                                  <  1:610859/151‑1 (MQ=255)
                                                                                ccTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTgtgt                                                                 >  1:321131/1‑151 (MQ=255)
                                                                                 cTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGtt                                                                >  2:218310/1‑151 (MQ=255)
                                                                                  tCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTg                                                               >  1:691886/1‑151 (MQ=255)
                                                                                  tCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTg                                                               >  1:100963/1‑151 (MQ=255)
                                                                                   ccccTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGa                                                              <  2:1232103/151‑1 (MQ=255)
                                                                                    cccTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGAt                                                             <  2:401514/151‑1 (MQ=255)
                                                                                    cccTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGAt                                                             <  1:756286/151‑1 (MQ=255)
                                                                                        gCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTTTTGATGTa                                                          <  2:1053658/150‑1 (MQ=255)
                                                                                        gCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGt                                                           <  1:1049841/149‑1 (MQ=255)
                                                                                        gCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTAt                                                         <  2:368396/151‑1 (MQ=255)
                                                                                         cATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATc                                                        >  2:147862/1‑151 (MQ=255)
                                                                                          aTTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATc                                                        >  1:907188/1‑150 (MQ=255)
                                                                                          aTTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATc                                                        >  2:688753/1‑150 (MQ=255)
                                                                                               gCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAAccc                                                  <  2:907227/151‑1 (MQ=255)
                                                                                                cAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCg                                                 <  1:875254/151‑1 (MQ=255)
                                                                                                 aaaaTCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGc                                                >  1:400196/1‑151 (MQ=255)
                                                                                                 aaaaTCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGc                                                <  2:1233083/151‑1 (MQ=255)
                                                                                                  aaaTCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCa                                               <  1:778692/151‑1 (MQ=255)
                                                                                                   aaTCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGc                                                >  1:259320/1‑149 (MQ=255)
                                                                                                      cAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAAc                                           <  2:555052/151‑1 (MQ=255)
                                                                                                       aGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAAc                                           >  2:638638/1‑150 (MQ=255)
                                                                                                             cACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCaa                                    <  1:621024/151‑1 (MQ=255)
                                                                                                                   cATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGc                              >  2:323930/1‑151 (MQ=255)
                                                                                                                   cATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGc                              >  2:435651/1‑151 (MQ=255)
                                                                                                                    aTGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGc                              >  2:1060435/1‑150 (MQ=255)
                                                                                                                        aaTTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATaa                         <  2:439295/151‑1 (MQ=255)
                                                                                                                         aTTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAAc                        <  2:933609/151‑1 (MQ=255)
                                                                                                                             tGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACAtt                     <  1:71098/150‑1 (MQ=255)
                                                                                                                              gATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTg                    <  2:1104926/150‑1 (MQ=255)
                                                                                                                              gATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTg                    <  2:1069631/150‑1 (MQ=255)
                                                                                                                               aTAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGc                   <  1:1221514/150‑1 (MQ=255)
                                                                                                                               aTAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCg                  <  2:849855/151‑1 (MQ=255)
                                                                                                                                 aaCGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCgg                 <  2:1245657/150‑1 (MQ=255)
                                                                                                                                 aaCGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCgg                 <  2:100965/150‑1 (MQ=255)
                                                                                                                                    gAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGt             <  2:1081873/151‑1 (MQ=255)
                                                                                                                                     aacaacGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTg            >  1:65020/1‑151 (MQ=255)
                                                                                                                                     aacaacGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTg            <  1:456724/151‑1 (MQ=255)
                                                                                                                                      acaacGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGc           <  2:315559/151‑1 (MQ=255)
                                                                                                                                         acGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATc        <  1:1080259/151‑1 (MQ=255)
                                                                                                                                          cGTTGACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCt       <  2:1041647/151‑1 (MQ=255)
                                                                                                                                              gACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCtttt    <  2:69827/150‑1 (MQ=255)
                                                                                                                                              gACCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCtttt    >  1:1171421/1‑150 (MQ=255)
                                                                                                                                               aCCTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGCTTTAACAGGAAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTa   >  1:3574/1‑150 (MQ=255)
                                                                                                                                                ccTTGGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTaa  >  2:328918/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAA  >  NC_000913/3247441‑3247734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑