Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,331,811	T→G	K309Q (AAG→CAG)	yhbE ←	EamA family inner membrane putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,331,811	0	T	G	100.0%	321.4 / NA	81	K309Q (AAG→CAG)	yhbE	EamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (46/35);  total (46/35)

TCGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAA  >  NC_000913/3331664‑3331950                                                                                                                                                    |                                                                                                                                            tCGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACg                                                                                                                                          >  2:1066628/1‑151 (MQ=255)  cGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGt                                                                                                                                         >  1:896514/1‑151 (MQ=255)   ggAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGt                                                                                                                                         >  1:46801/1‑150 (MQ=255)     aaTATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAAt                                                                                                                                      >  1:177959/1‑151 (MQ=255)        ataCTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCg                                                                                                                                   <  2:527625/151‑1 (MQ=255)           cTTTTCGCGGCGAAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCg                                                                                                                                   <  2:814245/148‑1 (MQ=255)            ttttCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGcccc                                                                                                                               >  1:258756/1‑151 (MQ=255)             tttCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGccc                                                                                                                                <  2:446663/149‑1 (MQ=255)                    gcggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGa                                                                                                                       <  1:681017/151‑1 (MQ=255)                     cggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAt                                                                                                                      >  1:1251184/1‑151 (MQ=255)                     cggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAt                                                                                                                      >  1:1301376/1‑151 (MQ=255)                     cggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAt                                                                                                                      >  2:720715/1‑151 (MQ=255)                      ggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAt                                                                                                                      <  2:1084897/150‑1 (MQ=255)                      ggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAt                                                                                                                      <  2:152539/150‑1 (MQ=255)                      ggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATg                                                                                                                     <  2:1100320/151‑1 (MQ=255)                           cTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCa                                                                                                                 >  2:493074/1‑150 (MQ=255)                             cACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAAt                                                                                                               <  1:1261305/150‑1 (MQ=255)                               cGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATgg                                                                                                             <  2:184661/150‑1 (MQ=255)                                 cAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCgg                                                                                                          >  1:730119/1‑151 (MQ=255)                                         aCCGCCATCACCTGCAACGACCAGAATGGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATc                                                                                                  >  2:514176/1‑151 (MQ=255)                                         aCCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATc                                                                                                  <  1:654623/151‑1 (MQ=255)                                          ccGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATcg                                                                                                 <  2:365351/151‑1 (MQ=255)                                             ccATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCgcgc                                                                                              >  1:21979/1‑151 (MQ=255)                                             ccATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCgcgc                                                                                              >  2:625673/1‑151 (MQ=255)                                                tCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGGCCAATGGCGGAATACATCGCGccc                                                                                            <  1:739949/150‑1 (MQ=255)                                                 cACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGc                                                                                          <  1:148301/151‑1 (MQ=255)                                                   ccTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCa                                                                                         <  2:1032290/150‑1 (MQ=255)                                                    cTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAAc                                                                                       >  2:503825/1‑151 (MQ=255)                                                    cTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCACAAATACGATGACCAATGGCGGAATACATCGCGCCCGCaa                                                                                        >  2:288856/1‑150 (MQ=255)                                                       cAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCAc                                                                                    >  1:430077/1‑151 (MQ=255)                                                             ccAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACaaa                                                                              <  1:319507/151‑1 (MQ=255)                                                             ccAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACaa                                                                               <  1:742548/150‑1 (MQ=255)                                                              cAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAAc                                                                             >  1:367053/1‑151 (MQ=255)                                                                 aaTCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGc                                                                           >  2:300116/1‑150 (MQ=255)                                                                    cGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCg                                                                       >  1:281539/1‑151 (MQ=255)                                                                      aTGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCg                                                                       >  1:1024251/1‑149 (MQ=255)                                                                      aTGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCg                                                                       >  2:1226952/1‑149 (MQ=255)                                                                      aTGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCg                                                                       >  2:819846/1‑149 (MQ=255)                                                                        gCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCgaga                                                                    <  1:820724/150‑1 (MQ=255)                                                                        gCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGAt                                                                   <  1:1066975/151‑1 (MQ=255)                                                                             atcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCt                                                              <  2:1193413/151‑1 (MQ=255)                                                                               caacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTa                                                             >  1:584077/1‑150 (MQ=255)                                                                                aacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTaaa                                                           <  2:1287095/151‑1 (MQ=255)                                                                                aacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTaaa                                                           <  1:537579/151‑1 (MQ=255)                                                                                 acaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTaaa                                                           >  1:617450/1‑150 (MQ=255)                                                                                 acaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTaaa                                                           >  1:1125912/1‑150 (MQ=255)                                                                                   aaaCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAgg                                                        >  1:736235/1‑151 (MQ=255)                                                                                   aaaCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAgg                                                        >  1:1127813/1‑151 (MQ=255)                                                                                     aCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGtt                                                      >  1:67585/1‑151 (MQ=255)                                                                                     aCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGtt                                                      >  2:1122040/1‑151 (MQ=255)                                                                                       ttCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGctt                                                     >  2:1085848/1‑150 (MQ=255)                                                                                          aTTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACa                                                 <  2:46802/151‑1 (MQ=255)                                                                                            tttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACAt                                                <  1:1282528/150‑1 (MQ=255)                                                                                            tttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATc                                               >  1:78995/1‑151 (MQ=255)                                                                                            tttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATc                                               >  2:1195495/1‑151 (MQ=255)                                                                                              tATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCgg                                             >  1:752287/1‑151 (MQ=255)                                                                                               aTTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGt                                            <  2:944056/151‑1 (MQ=255)                                                                                                ttCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTc                                           >  1:109678/1‑151 (MQ=255)                                                                                                 tctcCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTc                                           <  1:1112029/150‑1 (MQ=255)                                                                                                 tctcCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCt                                          >  1:44162/1‑151 (MQ=255)                                                                                                  ctcCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTg                                         >  1:329343/1‑151 (MQ=255)                                                                                                    ccGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCt                                          <  2:890506/148‑1 (MQ=255)                                                                                                     cGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCg                                      <  2:527409/151‑1 (MQ=255)                                                                                                        aaaTCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCga                                     >  2:1297208/1‑149 (MQ=255)                                                                                                        aaaTCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCga                                     >  2:58576/1‑149 (MQ=255)                                                                                                             aTTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATc                              <  1:349465/151‑1 (MQ=255)                                                                                                               tCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCg                             <  1:1193128/150‑1 (MQ=255)                                                                                                                cGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCgg                            <  1:716292/150‑1 (MQ=255)                                                                                                                    tGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcc                         >  1:574421/1‑149 (MQ=255)                                                                                                                     gcgcGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcca                        <  2:1225643/149‑1 (MQ=255)                                                                                                                     gcgcGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcca                        <  1:647449/149‑1 (MQ=255)                                                                                                                         ggggTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggccaggcc                    >  1:949940/1‑149 (MQ=255)                                                                                                                           ggTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTg                >  1:289715/1‑151 (MQ=255)                                                                                                                                cGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaa            >  2:1015321/1‑150 (MQ=255)                                                                                                                                   tACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaaa           >  1:496293/1‑148 (MQ=255)                                                                                                                                    accaccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGAt       <  1:197839/151‑1 (MQ=255)                                                                                                                                     ccaccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGa        <  1:954944/149‑1 (MQ=255)                                                                                                                                        ccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGa   <  1:1036850/151‑1 (MQ=255)                                                                                                                                         cGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGa   >  1:1157007/1‑150 (MQ=255)                                                                                                                                         cGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGa   >  2:388043/1‑150 (MQ=255)                                                                                                                                          gTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaa  >  2:1111726/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                            TCGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAA  >  NC_000913/3331664‑3331950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑