Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,348,462 T→G L4* (TTA→TGA)  yrbL → Mg(2+)‑starvation‑stimulated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,348,4620TG97.4% 280.1 / ‑0.9 78L4* (TTA→TGA) yrbLMg(2+)‑starvation‑stimulated protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (42/34);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-02

AATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTTATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGTACTACGCGC  >  NC_000913/3348314‑3348605
                                                                                                                                                    |                                                                                                                                               
aatcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGAt                                                                                                                                               >  2:947826/1‑151 (MQ=255)
 atcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATc                                                                                                                                              <  1:185720/151‑1 (MQ=255)
   caaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTg                                                                                                                                            <  2:109384/151‑1 (MQ=255)
    aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTg                                                                                                                                            >  1:595596/1‑150 (MQ=255)
    aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGa                                                                                                                                           <  1:655680/151‑1 (MQ=255)
     aCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGaa                                                                                                                                          <  2:576271/151‑1 (MQ=255)
      cGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAAc                                                                                                                                         >  2:396832/1‑151 (MQ=255)
        tCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACa                                                                                                                                        >  1:789284/1‑150 (MQ=255)
            ttGTACATTTTGTGCGCTTTTCACTTTCATAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGt                                                                                                                                    <  2:721317/150‑1 (MQ=255)
            ttGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGt                                                                                                                                    <  2:1242352/150‑1 (MQ=255)
             tGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTcc                                                                                                                                  >  1:348992/1‑151 (MQ=255)
              gTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTccc                                                                                                                                 <  2:1155645/151‑1 (MQ=255)
               tACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTcccc                                                                                                                                >  1:658096/1‑151 (MQ=255)
                 cATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTg                                                                                                                              <  2:778127/151‑1 (MQ=255)
                 cATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTg                                                                                                                              >  1:911054/1‑151 (MQ=255)
                  aTTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAGCCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTGAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCttg                                                                                                                             <  1:1185563/151‑3 (MQ=255)
                   ttttGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTggg                                                                                                                            >  1:968289/1‑151 (MQ=255)
                    tttGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTggg                                                                                                                            <  2:1033997/150‑1 (MQ=255)
                       gtgCGCTTTTCGCTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACAGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCAc                                                                                                                         <  2:501418/150‑1 (MQ=255)
                       gtgCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCAcc                                                                                                                        >  2:39129/1‑151 (MQ=255)
                            cTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGa                                                                                                                     >  1:1011292/1‑149 (MQ=255)
                            cTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGa                                                                                                                     >  2:777217/1‑149 (MQ=255)
                             ttttCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGa                                                                                                                     >  2:914571/1‑148 (MQ=255)
                              tttCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGAc                                                                                                                 >  1:686316/1‑151 (MQ=255)
                               ttCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGATCAAAGTCCCTTGGGCACCGGAAg                                                                                                                   >  1:964452/1‑148 (MQ=255)
                                  aCTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCg                                                                                                             <  2:324314/151‑1 (MQ=255)
                                     ttCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGa                                                                                                           <  1:425586/150‑1 (MQ=255)
                                      tCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGaa                                                                                                          <  1:1888/150‑1 (MQ=255)
                                       cAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAAt                                                                                                        <  2:252411/151‑1 (MQ=255)
                                       cAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAAt                                                                                                        <  1:1156067/151‑1 (MQ=255)
                                       cAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAAt                                                                                                        >  1:360062/1‑151 (MQ=255)
                                                   aaGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCAt                                                                                             <  1:39128/150‑1 (MQ=255)
                                                    aGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCAttc                                                                                           <  1:327012/151‑3 (MQ=255)
                                                    aGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATcc                                                                                           >  2:220629/1‑151 (MQ=255)
                                                    aGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATcc                                                                                           >  2:150427/1‑151 (MQ=255)
                                                       aaaCCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCgg                                                                                         >  1:1036954/1‑150 (MQ=255)
                                                         aCCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGTAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAg                                                                                      <  1:597285/151‑1 (MQ=255)
                                                         aCCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTTATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAg                                                                                      <  1:860596/151‑1 (MQ=255)
                                                         aCCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGa                                                                                        >  1:233189/1‑149 (MQ=255)
                                                            ttAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATg                                                                                   >  1:241926/1‑151 (MQ=255)
                                                             tAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATg                                                                                   <  2:339669/150‑1 (MQ=255)
                                                                gagGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCa                                                                               <  2:640778/151‑1 (MQ=255)
                                                                 agGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCaa                                                                              <  2:319984/151‑1 (MQ=255)
                                                                 agGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCa                                                                               >  1:589506/1‑150 (MQ=255)
                                                                     aTTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCt                                                                          >  2:688659/1‑151 (MQ=255)
                                                                     aTTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCt                                                                          >  1:103523/1‑151 (MQ=255)
                                                                      ttGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTg                                                                         >  1:603853/1‑151 (MQ=255)
                                                                       tGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGt                                                                        >  1:619437/1‑151 (MQ=255)
                                                                            aGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCa                                                                    <  2:309470/150‑1 (MQ=255)
                                                                                tttGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGAt                                                                <  2:1023595/150‑1 (MQ=255)
                                                                                  tGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTTATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGt                                                             >  1:1129918/1‑151 (MQ=255)
                                                                                     ttAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCt                                                           >  1:428352/1‑150 (MQ=255)
                                                                                      tAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTa                                                          <  1:1066764/150‑1 (MQ=255)
                                                                                       aaGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTAcc                                                        <  2:789434/151‑1 (MQ=255)
                                                                                           tAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCg                                                    >  1:75661/1‑151 (MQ=255)
                                                                                            aaTCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCg                                                    <  1:1009821/150‑1 (MQ=255)
                                                                                             aTCGACCATACTCGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCCCCTGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATc                                                     <  1:654562/148‑1 (MQ=255)
                                                                                                gACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCg                                               >  2:252847/1‑151 (MQ=255)
                                                                                                  ccATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGAt                                             <  1:1252769/151‑1 (MQ=255)
                                                                                                  ccATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGAt                                             >  1:283647/1‑151 (MQ=255)
                                                                                                  ccATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGAt                                             >  1:462917/1‑151 (MQ=255)
                                                                                                  ccATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGAt                                             >  2:361387/1‑151 (MQ=255)
                                                                                                    aTACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGAtgg                                           >  1:912817/1‑151 (MQ=255)
                                                                                                         ggAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCg                                      <  1:10055/151‑1 (MQ=255)
                                                                                                                gTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATaa                                  >  2:460168/1‑148 (MQ=255)
                                                                                                                  cAGAAAATATTTCAAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAag                                >  1:1227398/1‑148 (MQ=255)
                                                                                                                   aGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAag                                >  1:76329/1‑147 (MQ=255)
                                                                                                                   aGAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGAt                            <  1:671626/151‑1 (MQ=255)
                                                                                                                    gAAAATATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAag                                >  2:1125567/1‑146 (MQ=255)
                                                                                                                     aaaaTATTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATcc                          >  1:682956/1‑151 (MQ=255)
                                                                                                                          atTTCCAGGAGATGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATccgccg                      <  1:150425/150‑1 (MQ=255)
                                                                                                                                      tGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGtac         >  2:510039/1‑151 (MQ=255)
                                                                                                                                      tGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGtac         >  1:340006/1‑151 (MQ=255)
                                                                                                                                      tGGCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGtac         >  1:1211311/1‑151 (MQ=255)
                                                                                                                                       ggCATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAGCGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGtact        <  1:947768/151‑1 (MQ=255)
                                                                                                                                         cATGATTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGtactac      >  2:11266/1‑151 (MQ=255)
                                                                                                                                             aTTCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGTACTACgcgc  <  2:1098777/151‑1 (MQ=255)
                                                                                                                                              ttCGCTGATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGTACTACgcgc  >  1:240223/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTTTAAGTTAATCGACCATACTGGAGATCGTCAGAAAATATTTCCAGGAGATGGCATGATTCGCTTATCTGAACAAAGTCCCCTGGGCACCGGAAGACATCGGAAATGTTATGCGCATCCGGAAGATGCCCAACGCTGTATCAAGATTGTCTACCATCGTGGCGATGGTGGCGATAAAGAGATCCGCCGTGAGTTAAAGTACTACGCGC  >  NC_000913/3348314‑3348605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: