Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,987	G→T	G530C (GGT→TGT)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,987	0	G	T	100.0%	261.2 / NA	68	G530C (GGT→TGT)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/33);  total (35/33)

CTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTG  >  NC_000913/3823844‑3824134                                                                                                                                                |                                                                                                                                                    cTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGc                                                                                                                                              >  1:1036195/1‑151 (MQ=255)      aGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGa                                                                                                                                         <  1:1051731/151‑1 (MQ=255)      aGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGa                                                                                                                                         <  2:11755/151‑1 (MQ=255)        ccTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGc                                                                                                                                       <  2:939413/151‑1 (MQ=255)        ccTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGc                                                                                                                                       <  1:126850/151‑1 (MQ=255)           gggCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgtg                                                                                                                                    <  2:596862/151‑1 (MQ=255)            ggCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgtgg                                                                                                                                   <  2:496926/151‑1 (MQ=255)               cgtcgtCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGtggtgg                                                                                                                                <  2:299507/151‑1 (MQ=255)                gtcgtcTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTggtggt                                                                                                                               >  2:269518/1‑151 (MQ=255)                gtcgtcTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTggtggt                                                                                                                               <  2:274482/151‑1 (MQ=255)                            aCCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCt                                                                                                                   >  2:790308/1‑151 (MQ=255)                                    ttGGGTGGTAGCCGTAAGCTGAATGAAAGCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACa                                                                                                             <  1:74627/149‑1 (MQ=255)                                     tGGGCGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCCACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATgg                                                                                                          >  1:524499/1‑151 (MQ=255)                                             aGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGcc                                                                                                   <  1:256218/150‑1 (MQ=255)                                             aGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCt                                                                                                  <  2:688644/151‑1 (MQ=255)                                              gCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTc                                                                                                 <  2:1290020/151‑1 (MQ=255)                                               ccGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTcc                                                                                                >  2:191073/1‑151 (MQ=255)                                                    aGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTcc                                                                                           <  2:644274/151‑1 (MQ=255)                                                     gCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCa                                                                                          <  1:444017/151‑1 (MQ=255)                                                       tgaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcc                                                                                        >  1:382554/1‑151 (MQ=255)                                                       tgaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcc                                                                                        >  1:148629/1‑151 (MQ=255)                                                       tgaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcc                                                                                        >  2:509200/1‑151 (MQ=255)                                                       tgaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcc                                                                                        >  1:678641/1‑151 (MQ=255)                                                        gaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCg                                                                                       <  2:607826/151‑1 (MQ=255)                                                            gaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCa                                                                                    <  2:95762/150‑1 (MQ=255)                                                             aaaTCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAAc                                                                                  >  2:350966/1‑151 (MQ=255)                                                               aTCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAAccc                                                                                <  1:1229627/151‑1 (MQ=255)                                                                  ccGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCaaa                                                                             <  1:85745/151‑1 (MQ=255)                                                                  ccGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCaaa                                                                             <  2:711415/151‑1 (MQ=255)                                                                   cGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCa                                                                               <  2:1076648/148‑1 (MQ=255)                                                                     cAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGcc                                                                          >  2:570530/1‑151 (MQ=255)                                                                      aGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGcc                                                                          >  1:373391/1‑150 (MQ=255)                                                                        gAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACg                                                                       >  2:1171868/1‑151 (MQ=255)                                                                            atatTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGAc                                                                    >  1:166884/1‑150 (MQ=255)                                                                            atatTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGAc                                                                    >  1:1256934/1‑150 (MQ=255)                                                                              atTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATc                                                                 >  2:1206814/1‑151 (MQ=255)                                                                                     gcgAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCATTGAGCGTGGTGGACGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCa                                                           >  1:800718/1‑150 (MQ=255)                                                                                       gAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTa                                                        <  2:388158/151‑1 (MQ=255)                                                                                           tGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                    >  1:797583/1‑151 (MQ=255)                                                                                            ggATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                     <  1:431954/149‑1 (MQ=255)                                                                                             gATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTg                                                  >  1:628764/1‑151 (MQ=255)                                                                                             gATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTg                                                  <  1:431867/151‑1 (MQ=255)                                                                                                  cATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGAt                                             >  2:47603/1‑151 (MQ=255)                                                                                                         cTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGc                                      >  1:760352/1‑151 (MQ=255)                                                                                                           ggCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTg                                    <  1:453056/151‑1 (MQ=255)                                                                                                            gCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTg                                    >  2:621848/1‑150 (MQ=255)                                                                                                             cAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGAt                                  >  2:1110273/1‑151 (MQ=255)                                                                                                              aaCGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAAGGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATc                                 <  1:245564/151‑1 (MQ=255)                                                                                                               aCGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATc                                 >  2:359881/1‑150 (MQ=255)                                                                                                                    tGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCtt                            <  2:893425/150‑1 (MQ=255)                                                                                                                     gACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCttt                           <  2:727261/150‑1 (MQ=255)                                                                                                                     gACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCttt                           <  2:775116/150‑1 (MQ=255)                                                                                                                     gACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCttt                           <  1:1046255/150‑1 (MQ=255)                                                                                                                      aCGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGc                         <  2:1153305/151‑1 (MQ=255)                                                                                                                       cGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCg                        >  2:932550/1‑151 (MQ=255)                                                                                                                         aTCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGa                       >  1:440303/1‑150 (MQ=255)                                                                                                                           ctgctgGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAt                    >  2:127429/1‑151 (MQ=255)                                                                                                                             gctgGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGaaa                     <  2:1224327/148‑1 (MQ=255)                                                                                                                              ctgGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAtgct                 >  1:1082095/1‑151 (MQ=255)                                                                                                                                 gCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc              >  1:466006/1‑151 (MQ=255)                                                                                                                                    gAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgccgcc           <  2:163713/151‑1 (MQ=255)                                                                                                                                      aaTCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGccc          >  1:539567/1‑150 (MQ=255)                                                                                                                                       aTCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCt         >  2:1287529/1‑150 (MQ=255)                                                                                                                                       aTCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCt         >  1:1281724/1‑150 (MQ=255)                                                                                                                                         cGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTAtt      >  2:16895/1‑151 (MQ=255)                                                                                                                                         cGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTAt       <  2:1036259/150‑1 (MQ=255)                                                                                                                                            aCTTTGTAACGCAATGAGCGTGGTGGCCGCGAAAAATCAGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTc     >  1:13612/1‑149 (MQ=255)                                                                                                                                             cTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTg  >  1:154095/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    CTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTG  >  NC_000913/3823844‑3824134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑