Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,891,019	T→G	S262A (TCC→GCC)	tnaB →	tryptophan transporter of low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,891,019	0	T	G	100.0%	355.4 / NA	90	S262A (TCC→GCC)	tnaB	tryptophan transporter of low affinity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (46/44);  total (46/44)

CCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGG  >  NC_000913/3890872‑3891166                                                                                                                                                    |                                                                                                                                                    cccAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATcgcct                                                                                                                                                  <  1:55076/151‑1 (MQ=255)  ccAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGCTTATcgcctc                                                                                                                                                 >  1:640738/1‑151 (MQ=255)        tGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcgg                                                                                                                                           >  1:1106040/1‑151 (MQ=255)         gATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcggc                                                                                                                                          <  2:451767/151‑1 (MQ=255)         gATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcg                                                                                                                                            <  2:426461/149‑1 (MQ=255)          attattTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcggc                                                                                                                                          >  1:331981/1‑150 (MQ=255)          attattTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCa                                                                                                                                         <  1:1135520/151‑1 (MQ=255)            tattTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAAc                                                                                                                                       >  2:813119/1‑151 (MQ=255)             attTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAAc                                                                                                                                       <  1:618511/150‑1 (MQ=255)             attTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAAc                                                                                                                                       <  1:346558/150‑1 (MQ=255)             attTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACg                                                                                                                                      >  1:1020720/1‑151 (MQ=255)              tttGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGt                                                                                                                                     >  1:478844/1‑151 (MQ=255)                     tGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTc                                                                                                                               >  1:306044/1‑150 (MQ=255)                     tGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCg                                                                                                                              >  2:425346/1‑151 (MQ=255)                         aaaCGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTgg                                                                                                                          >  1:558609/1‑151 (MQ=255)                           aCGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTg                                                                                                                        >  1:728418/1‑151 (MQ=255)                            cGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTg                                                                                                                        >  1:910270/1‑150 (MQ=255)                            cGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTg                                                                                                                        >  2:1173574/1‑150 (MQ=255)                            cGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTg                                                                                                                        >  1:561003/1‑150 (MQ=255)                              cAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGaa                                                                                                                      >  2:355249/1‑150 (MQ=255)                              cAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGaa                                                                                                                      >  2:1111390/1‑150 (MQ=255)                               aaaGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAAt                                                                                                                    <  2:183724/151‑1 (MQ=255)                                  gATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGt                                                                                                                 <  2:1185095/151‑1 (MQ=255)                                      aGTTAATCAAAAGCGTGATATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCCCCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCATCGTTGATTCGCTGGTGAAATCGTTCCt                                                                                                             <  2:1141668/151‑1 (MQ=255)                                       gTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTc                                                                                                            >  2:855932/1‑151 (MQ=255)                                         tAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCg                                                                                                           <  2:883520/150‑1 (MQ=255)                                           aTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGc                                                                                                         >  2:1055171/1‑150 (MQ=255)                                            tCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAc                                                                                                       >  2:825245/1‑151 (MQ=255)                                            tCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAc                                                                                                       >  2:429865/1‑151 (MQ=255)                                             cAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAcc                                                                                                      >  2:446238/1‑151 (MQ=255)                                             cAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAc                                                                                                       <  2:597394/150‑1 (MQ=255)                                               aaaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCa                                                                                                     >  1:1009319/1‑150 (MQ=255)                                               aaaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCa                                                                                                     >  1:355018/1‑150 (MQ=255)                                               aaaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCa                                                                                                     >  1:751953/1‑150 (MQ=255)                                                aaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCaa                                                                                                    >  2:504084/1‑150 (MQ=255)                                                        tATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACgg                                                                                           >  1:107066/1‑151 (MQ=255)                                                          tttGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGc                                                                                          >  1:50084/1‑150 (MQ=255)                                                          tttGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGc                                                                                          <  1:966199/150‑1 (MQ=255)                                                                tCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATc                                                                                    <  1:1173209/150‑1 (MQ=255)                                                                 cGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGa                                                                                  <  2:204131/151‑1 (MQ=255)                                                                   ctgctgGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAg                                                                                 <  1:890310/150‑1 (MQ=255)                                                                     gctgGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGttt                                                                              <  1:966432/151‑1 (MQ=255)                                                                       tgGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTg                                                                            >  1:803759/1‑151 (MQ=255)                                                                        ggCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGtttt                                                                             <  1:712088/149‑1 (MQ=255)                                                                         gcgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGcc                                                                          <  2:520382/151‑1 (MQ=255)                                                                          cgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCct                                                                         >  2:858103/1‑151 (MQ=255)                                                                          cgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCct                                                                         <  2:815380/151‑1 (MQ=255)                                                                           gcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCctg                                                                        >  1:1131566/1‑151 (MQ=255)                                                                              ggTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCctgct                                                                      <  2:1007116/150‑1 (MQ=255)                                                                              ggTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCctgct                                                                      <  1:590662/150‑1 (MQ=255)                                                                               gTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTgg                                                                    <  2:1108330/151‑1 (MQ=255)                                                                                 gATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGgtg                                                                  <  2:1249734/151‑1 (MQ=255)                                                                                 gATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGgtg                                                                  <  2:10441/151‑1 (MQ=255)                                                                                      aTCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTtctc                                                             >  1:1244747/1‑151 (MQ=255)                                                                                        ctctTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCCTTATCGAGTTTTGCCTGCTGGTGTTctct                                                            >  2:703740/1‑150 (MQ=255)                                                                                            tctGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAAc                                                         <  2:1107120/149‑1 (MQ=255)                                                                                               ggCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTa                                                      <  2:469702/149‑1 (MQ=255)                                                                                               ggCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAg                                                     <  2:369610/150‑1 (MQ=255)                                                                                                   ctATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGtt                                                >  2:400957/1‑151 (MQ=255)                                                                                                     aTTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGtt                                                <  2:151120/149‑1 (MQ=255)                                                                                                     aTTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGt                                                 <  2:135383/148‑1 (MQ=255)                                                                                                     aTTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTg                                               <  1:798073/150‑1 (MQ=255)                                                                                                       tGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGcc                                             >  1:178793/1‑150 (MQ=255)                                                                                                        gCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCa                                            <  2:803791/150‑1 (MQ=255)                                                                                                         cACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgt                                          <  2:184952/151‑1 (MQ=255)                                                                                                            cATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcg                                       <  2:369562/151‑1 (MQ=255)                                                                                                            cATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcg                                       <  2:446051/151‑1 (MQ=255)                                                                                                             aTGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcgt                                      >  1:483895/1‑151 (MQ=255)                                                                                                               ggggAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcgttc                                    <  1:224071/151‑1 (MQ=255)                                                                                                               ggggAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcgtt                                     <  1:1096034/150‑1 (MQ=255)                                                                                                                 ggAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCtt                                  <  1:836722/151‑1 (MQ=255)                                                                                                                   aaTATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTg                                <  2:468638/151‑1 (MQ=255)                                                                                                                   aaTATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTg                                >  2:794452/1‑151 (MQ=255)                                                                                                                   aaTATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGGGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCttt                                 >  2:8051/1‑150 (MQ=255)                                                                                                                         ccGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTc                           >  2:415290/1‑150 (MQ=255)                                                                                                                          cgcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCAc                         <  2:561019/151‑1 (MQ=255)                                                                                                                           gcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTTCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACg                        >  2:109989/1‑151 (MQ=255)                                                                                                                           gcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACg                        >  1:1287887/1‑151 (MQ=255)                                                                                                                           gcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACg                        >  2:959693/1‑151 (MQ=255)                                                                                                                             gagaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGCGTCACGCt                      >  2:520056/1‑151 (MQ=255)                                                                                                                              agaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTg                     <  2:132860/151‑1 (MQ=255)                                                                                                                              agaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTg                     <  2:775008/151‑1 (MQ=255)                                                                                                                                   gCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgtt                >  1:841685/1‑151 (MQ=255)                                                                                                                                     tttAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgttgt              >  1:515298/1‑151 (MQ=255)                                                                                                                                     tttAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgttgt              >  2:984581/1‑151 (MQ=255)                                                                                                                                      ttAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgttg               <  1:491046/149‑1 (MQ=255)                                                                                                                                      ttAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgttg               <  1:799724/149‑1 (MQ=255)                                                                                                                                       tAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgttgtt             >  1:919633/1‑150 (MQ=255)                                                                                                                                       tAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTc            >  2:63609/1‑151 (MQ=255)                                                                                                                                          ggCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGAt         <  2:216348/151‑1 (MQ=255)                                                                                                                                                tATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTg   >  1:548325/1‑151 (MQ=255)                                                                                                                                                 aTCGCCTCAGGCGGCAACGTTGATTCGCTGGTAAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTgg  <  2:673125/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGG  >  NC_000913/3890872‑3891166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑