Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,860	A→C	F107V (TTT→GTT)	rarD ←	putative chloramphenical resistance permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,860	0	A	C	100.0%	317.0 / NA	80	F107V (TTT→GTT)	rarD	putative chloramphenical resistance permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (48/32);  total (48/32)

CCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGC  >  NC_000913/4003712‑4004006                                                                                                                                                     |                                                                                                                                                   cccAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGt                                                                                                                                                  <  1:1295700/151‑1 (MQ=255)  ccAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGt                                                                                                                                                  >  1:80295/1‑150 (MQ=255)  ccAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTa                                                                                                                                                 <  2:8306/151‑1 (MQ=255)        cgATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAg                                                                                                                                           <  2:1045188/151‑1 (MQ=255)            aataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTc                                                                                                                                       >  2:903907/1‑151 (MQ=255)            aataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT‑CCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTc                                                                                                                                       <  2:489292/150‑1 (MQ=255)             ataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCg                                                                                                                                      <  2:97908/151‑1 (MQ=255)                ggTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCtt                                                                                                                                   <  2:330596/151‑1 (MQ=255)                  tAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTcc                                                                                                                                 >  1:245197/1‑151 (MQ=255)                   aGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTcc                                                                                                                                 >  1:386814/1‑150 (MQ=255)                   aGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTcc                                                                                                                                 >  2:302028/1‑150 (MQ=255)                     cGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCTAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACACTGTTCACCAGCGTGTCAATAACGTAACCAAGGCTCTCTTcga                                                                                                                                >  2:807053/1‑147 (MQ=255)                      gAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGc                                                                                                                              >  1:422488/1‑150 (MQ=255)                       aaCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCat                                                                                                                            >  1:668104/1‑151 (MQ=255)                       aaCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCat                                                                                                                            <  2:861155/151‑1 (MQ=255)                         ccAAAAGTCCACAGCGGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCatat                                                                                                                          <  1:939778/151‑1 (MQ=255)                           aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATCACGTAACCAAGGCTCGCTTCCAGCATATgg                                                                                                                        >  2:735404/1‑151 (MQ=255)                                     aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTc                                                                                                               >  1:297003/1‑150 (MQ=255)                                        tGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                                                                           >  1:589200/1‑151 (MQ=255)                                             tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                                                                        >  2:913235/1‑149 (MQ=255)                                              aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCa                                                                                                       <  1:1141542/149‑1 (MQ=255)                                                  cGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGAt                                                                                                    >  1:874210/1‑148 (MQ=255)                                                   gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAAc                                                                                                >  2:515847/1‑151 (MQ=255)                                                    ccACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAAc                                                                                                >  1:736994/1‑150 (MQ=255)                                                       caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                                                             >  1:1215461/1‑150 (MQ=255)                                                       caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTa                                                                                            <  1:135919/151‑1 (MQ=255)                                                       caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGCGATTGTTCACCGCCCAGATAAACAGt                                                                                             >  2:988647/1‑150 (MQ=255)                                                        atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                                                             >  2:96505/1‑149 (MQ=255)                                                        atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                                                                           <  2:693757/151‑1 (MQ=255)                                                             gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGt                                                                                      >  1:680461/1‑151 (MQ=255)                                                             gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGt                                                                                      >  2:911769/1‑151 (MQ=255)                                                              ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCCCCGCCCAGATAAACAGTAGCCAGtt                                                                                     <  2:662157/151‑1 (MQ=255)                                                              ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGt                                                                                      >  1:594082/1‑150 (MQ=255)                                                                aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGc                                                                                   >  2:53683/1‑151 (MQ=255)                                                                      aCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacca                                                                              >  1:371713/1‑150 (MQ=255)                                                                          ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATc                                                                           >  1:898914/1‑149 (MQ=255)                                                                           cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                                        >  2:442746/1‑151 (MQ=255)                                                                            agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                                        >  1:486267/1‑150 (MQ=255)                                                                            agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                                        >  2:942162/1‑150 (MQ=255)                                                                                   gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGc                                                                   >  2:89557/1‑148 (MQ=255)                                                                                       ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAc                                                             <  1:456020/150‑1 (MQ=255)                                                                                           cggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGcc                                                         >  2:631362/1‑150 (MQ=255)                                                                                               aTCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCAt                                                    <  2:642490/151‑1 (MQ=255)                                                                                                     cgcCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaaa                                               <  2:108342/150‑1 (MQ=255)                                                                                                      gcCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAtt                                             >  2:57227/1‑151 (MQ=255)                                                                                                      gcCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAtt                                             >  2:395509/1‑151 (MQ=255)                                                                                                       ccGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGGTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAttt                                            >  1:328322/1‑151 (MQ=255)                                                                                                        cGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAtttt                                           <  2:965563/151‑1 (MQ=255)                                                                                                         gAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTc                                          <  2:1252111/151‑1 (MQ=255)                                                                                                         gAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTc                                          >  2:618675/1‑151 (MQ=255)                                                                                                         gAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTc                                          >  1:1044963/1‑151 (MQ=255)                                                                                                           ggAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCtg                                        >  2:1070186/1‑151 (MQ=255)                                                                                                              aaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTgg                                     >  1:1155758/1‑151 (MQ=255)                                                                                                                  catcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt                                  >  1:1139100/1‑150 (MQ=255)                                                                                                                  catcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt                                  <  1:738025/150‑1 (MQ=255)                                                                                                                  catcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTc                                 <  1:789458/151‑1 (MQ=255)                                                                                                                   atcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCt                                >  1:217178/1‑151 (MQ=255)                                                                                                                     ccccAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTg                               <  2:23150/150‑1 (MQ=255)                                                                                                                        cAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATc                           <  1:885445/151‑1 (MQ=255)                                                                                                                          gCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATc                           >  2:299423/1‑149 (MQ=255)                                                                                                                               aTGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttt                    <  1:111777/151‑1 (MQ=255)                                                                                                                               aTGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttt                    <  1:848709/151‑1 (MQ=255)                                                                                                                               aTGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttt                    >  2:388955/1‑151 (MQ=255)                                                                                                                                tGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGtttt                   >  1:889904/1‑151 (MQ=255)                                                                                                                                 gTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGtttt                   <  2:117388/150‑1 (MQ=255)                                                                                                                                  ttCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttttt                  >  2:277404/1‑150 (MQ=255)                                                                                                                                  ttCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttttt                  >  1:758737/1‑150 (MQ=255)                                                                                                                                  ttCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttttt                  >  1:1167728/1‑150 (MQ=255)                                                                                                                                    cACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTaa                >  1:1173518/1‑150 (MQ=255)                                                                                                                                     aCCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAAt              <  1:794304/151‑1 (MQ=255)                                                                                                                                       cAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAAt              <  2:1160505/149‑1 (MQ=255)                                                                                                                                        aGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAgg           >  2:54964/1‑151 (MQ=255)                                                                                                                                         gCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAgg           <  1:5179/150‑1 (MQ=255)                                                                                                                                          cGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGAc         >  1:388087/1‑151 (MQ=255)                                                                                                                                          cGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGAc         <  2:53843/151‑1 (MQ=255)                                                                                                                                          cGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTACCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGAc         <  1:152559/151‑1 (MQ=255)                                                                                                                                            ggTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCa       <  1:214458/151‑1 (MQ=255)                                                                                                                                                aataaCGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTgg   <  2:560395/151‑1 (MQ=255)                                                                                                                                                aataaCGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTgg   >  2:56797/1‑151 (MQ=255)                                                                                                                                                 ataaCGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCGCCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTggc  <  2:792059/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGC  >  NC_000913/4003712‑4004006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑