Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,290,993 A→C Y173S (TAT→TCT)  nrfD → formate‑dependent nitrite reductase, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,290,9930AC98.4% 202.9 / NA 65Y173S (TAT→TCT) nrfDformate‑dependent nitrite reductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (23/40);  minor base T (0/1);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAACGCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAAT  >  NC_000913/4290846‑4291140
                                                                                                                                                   |                                                                                                                                                   
aacaacGCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTTTTGTTTGGGGCTTTTACCCGCTTTCTGCTGTCGGGGCTGCAATCGTCTcc                                                                                                                                                  >  1:863246/1‑151 (MQ=255)
      gCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTTTGCTGTCGGCGCTGAAATCGTCTCCGtt                                                                                                                                               >  2:416826/1‑148 (MQ=255)
            tGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATa                                                                                                                                       <  1:310546/150‑1 (MQ=255)
             gCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATaa                                                                                                                                      <  1:637668/150‑1 (MQ=255)
              ccGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAAc                                                                                                                                     <  2:63092/150‑1 (MQ=255)
                gAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCg                                                                                                                                  <  2:1220038/151‑1 (MQ=255)
                gAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCg                                                                                                                                  <  2:220927/151‑1 (MQ=255)
                gAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGGTGTTGGGGGCTTATACCCGCTTTCTGCTGTCGGCGCTGGAATCGTCTCCGTTCCTCAATAAccc                                                                                                                                   >  1:70309/1‑150 (MQ=255)
                 aaGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTTCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAAccc                                                                                                                                   >  2:307143/1‑149 (MQ=255)
                      ggggATCGTGCAAAAGGTTCTGAGCCTGCGGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCt                                                                                                                             <  2:737511/150‑1 (MQ=255)
                            cGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGt                                                                                                                       <  2:1129156/150‑1 (MQ=255)
                                 aaaaGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTTCTCAATAACCCGATCCTGCCGGTGCTGtt                                                                                                                 >  1:1238916/1‑151 (MQ=255)
                                 aaaaGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGGGCTGtt                                                                                                                 >  2:1010224/1‑151 (MQ=255)
                                         cTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGTGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCtctt                                                                                                           >  2:1208889/1‑149 (MQ=255)
                                          tGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGGGCTGTTCCtcttct                                                                                                         >  2:1274097/1‑150 (MQ=255)
                                           gAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCtcttct                                                                                                         <  1:1030788/149‑1 (MQ=255)
                                            aGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTTCTCTTCTcc                                                                                                       >  2:1155171/1‑150 (MQ=255)
                                            aGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGGTGTTGAGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGGCTCCGTTCCTCCATAACCCGATCCTGCCGGGGCTGTTCCTCTTCTcc                                                                                                       >  1:697256/1‑150 (MQ=255)
                                               ctgctgACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGCGTTTTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGGGGTGTTCCTCTTCTCCgg                                                                                                     >  1:580051/1‑149 (MQ=255)
                                                    gACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCAtct                                                                                                <  2:853244/149‑1 (MQ=255)
                                                    gACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCAtct                                                                                                <  2:315869/149‑1 (MQ=255)
                                                    gACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCAtct                                                                                                <  2:852002/149‑1 (MQ=255)
                                                    gACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCAtct                                                                                                <  1:260351/149‑1 (MQ=255)
                                                            ttCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCCTATACCGGCTTTCTGCTTTAGGGGGTGGAATCGTCTCTGTTTCTCCATAAACCTCTCCTGCCGGGGGTGGTCCCCTTCTCCGGCGTCTCGTCCCGt                                                                                        >  1:203581/1‑149 (MQ=255)
                                                            ttCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGAGTTGTTTGGGGCTTATACCGGCTTTCTGGTGGCGGCGCTTGAATCGTCTCCGGTCCTCAATAACCCGATCCTGGCGGTCATGTTTCACTTCTCCGGCCTCTCGTCCGGTg                                                                                       >  1:1103131/1‑150 (MQ=255)
                                                                  gcgGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGt                                                                                <  1:145412/151‑1 (MQ=255)
                                                                   cgGACTGGAAACATTGATGCTGGTGCTGGCGGTGGTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAAACCCATCCCGCCGGTGCTGTTTCTCTTCTCCGGCATCTCGTCCCGTGcggcgggg                                                                               >  2:201528/1‑149 (MQ=255)
                                                                      aCTGGAAACATTGATGCTGGTGCTGGCGGGGTTGTTGGGGGCATATACACGCTTTCCGCTGTCGGCGCTGAAATCGCGCCACTTCCTCCATAACCCCACCCTGCCCGGGCTGTTCCTATTCTCCGGCATCTCCGCCGGGGcggcggg                                                                                >  1:384941/1‑146 (MQ=255)
                                                                        tGGAAACATTGATGCTGGTGCTGGCGGTGGTGTTGGGGGCCTATAACGTCCTTCTGCTGGCGGCGCTTTAATCGTCTCCGTTTCTCAATAACCGGATCCTGCCGGTGCTTTTCCTTTTTTCCGGCCACTCGTCCGGGGcggcgg                                                                                 >  2:431265/1‑144 (MQ=255)
                                                                          gAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGGGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGgggggg                                                                            >  2:1145864/1‑145 (MQ=255)
                                                                            aaCATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATc                                                                      <  2:225552/151‑1 (MQ=255)
                                                                                 tGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCa                                                                  <  1:364053/150‑1 (MQ=255)
                                                                                 tGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCa                                                                  <  1:416816/150‑1 (MQ=255)
                                                                                    tgctggtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATgg                                                               <  2:786686/150‑1 (MQ=255)
                                                                                        ggtgctggCGGCGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGGGGGGCTGGTCGCCCTgggggt                                                           >  1:441686/1‑146 (MQ=255)
                                                                                           gctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACg                                                        <  1:483651/150‑1 (MQ=255)
                                                                                               gCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAAc                                                   <  1:1136443/151‑1 (MQ=255)
                                                                                               gCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAAc                                                   <  1:621352/151‑1 (MQ=255)
                                                                                               gCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGGGGGGCCGGTCGCCATGGGGAGACGcc                                                      >  1:97399/1‑148 (MQ=255)
                                                                                                 ggTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGc                                                 <  1:131032/151‑1 (MQ=255)
                                                                                                    gttgttGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGt                                              <  2:11194/151‑1 (MQ=255)
                                                                                                       gttgGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTaa                                            <  2:1105063/150‑1 (MQ=255)
                                                                                                         tgGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAAcc                                          <  2:477578/150‑1 (MQ=255)
                                                                                                          gggggCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAAccc                                         <  2:1238992/150‑1 (MQ=255)
                                                                                                           ggggCTTATACCGGCTTTCGGCCGTCGGGGCTGAAACCGTCTCCGTTCCTCACTAGCCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCgg                                       <  1:212980/151‑2 (MQ=255)
                                                                                                             ggCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCAt                                     <  2:1229354/151‑1 (MQ=255)
                                                                                                                 tataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGGTCGCCATGGCGGTAACCCCACGCAGTAAccctcctt                                    >  2:717810/1‑143 (MQ=255)
                                                                                                                 tataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTc                                   <  1:839900/149‑1 (MQ=255)
                                                                                                                 tataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGGAAACCGCATTcc                                  >  1:686923/1‑150 (MQ=255)
                                                                                                                  ataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCa                                 <  2:1250584/150‑1 (MQ=255)
                                                                                                                      cGGCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGaa                            <  2:23774/151‑1 (MQ=255)
                                                                                                                       ggCTTTCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGCGGCGCCGATCGCCATGGCGATACGCCAACGCAGTAAACCGCATTCCACgg                              >  2:799186/1‑148 (MQ=255)
                                                                                                                           ttCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGGTCGCCACGGCGATACGCCCACGCCGTAAACCGCCTTCCACGGAAgcg                         >  2:797916/1‑149 (MQ=255)
                                                                                                                            tCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGTTCGCCCTGGCGATACGCCAACGCAGTAACCCGCATTACACGGCAgcgc                        >  1:646376/1‑149 (MQ=255)
                                                                                                                            tCTGCTGTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCa                       <  2:499107/150‑1 (MQ=255)
                                                                                                                               gctgTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAg                      <  2:134239/148‑1 (MQ=255)
                                                                                                                                 tctcgccccTGAAATCTTTTCCGTTCCTCAATACCCCGTTCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGTTGGCGCTGATCGCCACGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAg                      <  1:1063276/138‑1 (MQ=255)
                                                                                                                                  gTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGTAGTAACCCGCATTCCACGGAAGCGCAGTTTGt                 <  1:634263/150‑1 (MQ=255)
                                                                                                                                  gTCGGCGCTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGt                 <  2:861576/150‑1 (MQ=255)
                                                                                                                                        gcTGAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCg           <  1:848089/150‑1 (MQ=255)
                                                                                                                                           gAAATCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTAt        <  1:614789/150‑1 (MQ=255)
                                                                                                                                            aaaTCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATgg      <  1:1207225/151‑1 (MQ=255)
                                                                                                                                             aaTCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGa     <  1:1008302/151‑1 (MQ=255)
                                                                                                                                               tCGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGaa    <  1:1155098/150‑1 (MQ=255)
                                                                                                                                                cGTCTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAAt  <  1:254340/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACAACGCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTGCTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAAT  >  NC_000913/4290846‑4291140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: