Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,164	T→G	E81D (GAA→GAC)	idnD ←	L‑idonate 5‑dehydrogenase, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,164	0	T	G	100.0%	305.9 / NA	78	E81D (GAA→GAC)	idnD	L‑idonate 5‑dehydrogenase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (43/35);  total (43/35)

AACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCTTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTG  >  NC_000913/4494022‑4494310                                                                                                                                               |                                                                                                                                                   aaCGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTa                                                                                                                                             >  1:13660/1‑150 (MQ=255)  aCGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAAt                                                                                                                                           <  1:1045177/151‑1 (MQ=255)  aCGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAAt                                                                                                                                           <  1:689253/151‑1 (MQ=255)   cGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAAtt                                                                                                                                          <  2:325129/151‑1 (MQ=255)      gTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCtg                                                                                                                                       <  2:520592/151‑1 (MQ=255)           accaccATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCtgatg                                                                                                                                    >  2:542188/1‑149 (MQ=255)             caccaTCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGt                                                                                                                                 >  2:771950/1‑150 (MQ=255)                   cAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATg                                                                                                                          >  1:348235/1‑151 (MQ=255)                   cAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATg                                                                                                                          >  1:1251899/1‑151 (MQ=255)                    aaCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATg                                                                                                                          >  2:56776/1‑150 (MQ=255)                     aCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGaa                                                                                                                        <  2:1117064/151‑1 (MQ=255)                       aTGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATa                                                                                                                      <  2:878312/151‑1 (MQ=255)                          aGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACt                                                                                                                   <  1:1267934/151‑1 (MQ=255)                             gAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACttt                                                                                                                 >  2:775179/1‑150 (MQ=255)                             gAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACttt                                                                                                                 >  1:617549/1‑150 (MQ=255)                                     tGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAAc                                                                                                        >  2:1081180/1‑151 (MQ=255)                                       gCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTTATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACtt                                                                                                      <  2:481951/151‑1 (MQ=255)                                               aaaaaaaCGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCt                                                                                              <  2:92418/151‑1 (MQ=255)                                                aaaaaaCGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCt                                                                                              >  2:433268/1‑150 (MQ=255)                                                 aaaaaCGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTa                                                                                             >  1:1136973/1‑150 (MQ=255)                                                  aaaaCGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAac                                                                                           >  2:411629/1‑151 (MQ=255)                                                     aCGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACAcc                                                                                        <  1:1272166/151‑1 (MQ=255)                                                         atatCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCg                                                                                    >  1:593869/1‑151 (MQ=255)                                                          tatCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCgg                                                                                   >  1:9711/1‑151 (MQ=255)                                                          tatCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCgg                                                                                   >  1:1250820/1‑151 (MQ=255)                                                           atCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCgg                                                                                   <  2:425974/150‑1 (MQ=255)                                                                 acacTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCttt                                                                            >  1:848802/1‑151 (MQ=255)                                                                   acTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTAt                                                                          <  2:1057983/151‑1 (MQ=255)                                                                     tGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATc                                                                         >  1:1036840/1‑150 (MQ=255)                                                                            cGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGaaa                                                                   <  2:596030/149‑1 (MQ=255)                                                                              ttATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCa                                                                        >  1:1299552/1‑142 (MQ=255)                                                                              ttATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCa                                                                        <  2:1299633/142‑1 (MQ=255)                                                                              ttATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTAc                                                               >  1:1264474/1‑151 (MQ=255)                                                                                 tGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCt                                                             >  1:20791/1‑150 (MQ=255)                                                                                      aaTGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACtttt                                                       <  2:186047/151‑1 (MQ=255)                                                                                       aTGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttc                                                      >  2:987351/1‑151 (MQ=255)                                                                                         gCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttcct                                                    >  1:818044/1‑151 (MQ=255)                                                                                         gCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttcc                                                     <  2:233141/150‑1 (MQ=255)                                                                                          cAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttcctt                                                   <  1:675031/151‑1 (MQ=255)                                                                                           aGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttccttc                                                  <  1:764634/151‑1 (MQ=255)                                                                                           aGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttccttc                                                  >  2:565733/1‑151 (MQ=255)                                                                                            gTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttccttcc                                                 <  2:871738/151‑1 (MQ=255)                                                                                            gTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttccttcc                                                 >  1:1263872/1‑151 (MQ=255)                                                                                            gTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTttccttcc                                                 <  2:619479/151‑1 (MQ=255)                                                                                              aTTTGCAGTGACCGCTCGGTTTAGACGGATTAATGGCTCCCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGGCCTAACGCCATCGGTGCCTTTATCATGAAATTACCTTCTTTTcctgcctg                                               <  1:667569/151‑1 (MQ=255)                                                                                              aTTTGCAGTGACCGCACGGTTTAGACGGATTAATTGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTg                                               <  1:195303/151‑1 (MQ=255)                                                                                                ttGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGa                                              <  1:846861/150‑1 (MQ=255)                                                                                                 tGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGat                                             >  1:124289/1‑150 (MQ=255)                                                                                                 tGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGat                                             <  2:1230081/150‑1 (MQ=255)                                                                                                  gCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGata                                            >  2:523611/1‑150 (MQ=255)                                                                                                         ccGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGt                                     >  2:471007/1‑150 (MQ=255)                                                                                                            cACGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAAt                                 >  2:21000/1‑151 (MQ=255)                                                                                                              cGGTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCg                               <  1:616573/151‑1 (MQ=255)                                                                                                               ggTTTAGACGGATTAATGGCTAGCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCgg                              >  1:1021591/1‑151 (MQ=255)                                                                                                                gTTTAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGa                             <  1:874727/151‑1 (MQ=255)                                                                                                                 tttAGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGa                             >  2:1011908/1‑150 (MQ=255)                                                                                                                    aGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAAcc                          >  2:704536/1‑150 (MQ=255)                                                                                                                    aGACGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCg                         <  2:895990/151‑1 (MQ=255)                                                                                                                      aCGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGc                        >  2:359698/1‑150 (MQ=255)                                                                                                                      aCGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGc                        >  2:1058009/1‑150 (MQ=255)                                                                                                                       cGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCaa                      <  2:926825/151‑1 (MQ=255)                                                                                                                       cGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCa                       >  1:605273/1‑150 (MQ=255)                                                                                                                       cGGATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCa                       >  1:1166005/1‑150 (MQ=255)                                                                                                                        ggATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCaaa                     <  1:663734/151‑1 (MQ=255)                                                                                                                        ggATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCaa                      >  1:77540/1‑150 (MQ=255)                                                                                                                         gATTAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAAt                    <  1:699747/151‑1 (MQ=255)                                                                                                                            tAATGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTcc                 >  1:1033630/1‑151 (MQ=255)                                                                                                                             aaTGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTcc                 >  2:476179/1‑150 (MQ=255)                                                                                                                             aaTGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTcc                 >  2:416350/1‑150 (MQ=255)                                                                                                                              aTGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCAc               >  1:432409/1‑151 (MQ=255)                                                                                                                               tGGCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCAcc              >  2:1102881/1‑151 (MQ=255)                                                                                                                                ggCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCt             <  2:300786/151‑1 (MQ=255)                                                                                                                                 gCTACCGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACCCCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCctcctc            <  1:577746/151‑1 (MQ=255)                                                                                                                                     ccGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGt        <  2:1185808/151‑1 (MQ=255)                                                                                                                                     ccGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGt        >  2:931410/1‑151 (MQ=255)                                                                                                                                      cGTTTGCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGtt       >  1:62273/1‑151 (MQ=255)                                                                                                                                           gCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTAtgtg  <  2:781922/151‑1 (MQ=255)                                                                                                                                           gCCCGTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTAtgtg  <  1:207579/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   AACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTTGCCCTTCATGTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTG  >  NC_000913/4494022‑4494310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑