Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,628,246 A→C D311A (GAT→GCT)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,628,2460AC98.9% 334.8 / NA 89D311A (GAT→GCT) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (43/45);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCA  >  NC_000913/4628099‑4628395
                                                                                                                                                   |                                                                                                                                                     
aaTATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCCCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCttt                                                                                                                                                    >  1:1099326/1‑151 (MQ=255)
aaTATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCttt                                                                                                                                                    <  1:110330/151‑1 (MQ=255)
 atatGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCtttt                                                                                                                                                   <  1:502554/151‑1 (MQ=255)
            aTTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCa                                                                                                                                        <  2:1214966/151‑1 (MQ=255)
              tATGTCTTTTCACACCTCGGCGGTGGTGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCa                                                                                                                                        >  2:435218/1‑149 (MQ=255)
               aTGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAgg                                                                                                                                      <  1:756196/150‑1 (MQ=255)
               aTGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGc                                                                                                                                     <  1:18500/151‑1 (MQ=255)
                  tCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGt                                                                                                                                   <  1:464465/150‑1 (MQ=255)
                    ttttCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGtt                                                                                                                                  >  1:1037955/1‑149 (MQ=255)
                    ttctCACACCTCCGCGGTTATGAGATCGCATTGCAGTATTCTGACTACGATACAATCGCGCTGGGCCACGCTCAATACCTTGATTTTGCGGTGAAATATGCCAATAAGGTGGCATTTATCGATACCGCTTTTGTCGCCACTCAGGCGTTc                                                                                                                                 <  1:1020706/147‑1 (MQ=255)
                      ttCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTg                                                                                                                               >  2:211554/1‑150 (MQ=255)
                        cacaCCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCa                                                                                                                             >  1:1178570/1‑150 (MQ=255)
                         acacCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCaaa                                                                                                                           <  2:259389/151‑1 (MQ=255)
                         acacCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAATGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCaa                                                                                                                            >  2:938741/1‑150 (MQ=255)
                          cacCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCaaaa                                                                                                                          <  2:445929/151‑1 (MQ=255)
                                ggcggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACg                                                                                                                    <  1:55680/151‑1 (MQ=255)
                                    gTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTCCTGCAAAAAGTACGAAgg                                                                                                                <  2:1046606/151‑1 (MQ=255)
                                     tgatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAggg                                                                                                               >  2:742162/1‑151 (MQ=255)
                                      gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGc                                                                                                              >  2:153198/1‑151 (MQ=255)
                                           gaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGa                                                                                                          >  1:352225/1‑150 (MQ=255)
                                              cGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACAt                                                                                                      <  2:343403/151‑1 (MQ=255)
                                              cGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACAt                                                                                                      <  1:609067/151‑1 (MQ=255)
                                               gCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATc                                                                                                     >  2:855748/1‑151 (MQ=255)
                                                     cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTc                                                                                                <  1:1205557/150‑1 (MQ=255)
                                                     cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCg                                                                                               >  1:871355/1‑151 (MQ=255)
                                                     cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCg                                                                                               >  1:522872/1‑151 (MQ=255)
                                                       gTATTCTGACTACGATAAAATCGAGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGAGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTg                                                                                             >  1:656930/1‑151 (MQ=255)
                                                           tCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAg                                                                                          <  1:456927/150‑1 (MQ=255)
                                                              gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgc                                                                                        <  1:742954/149‑1 (MQ=255)
                                                                   cGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgatt                                                                                 <  1:190064/151‑1 (MQ=255)
                                                                         aaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATtgatga                                                                            >  1:343548/1‑150 (MQ=255)
                                                                         aaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGaa                                                                           <  2:1104090/151‑1 (MQ=255)
                                                                          aTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAAt                                                                          <  2:861018/151‑1 (MQ=255)
                                                                             gcgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATAcc                                                                       >  2:32990/1‑151 (MQ=255)
                                                                              cgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCg                                                                      >  1:784012/1‑151 (MQ=255)
                                                                               gcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCg                                                                      >  2:23130/1‑150 (MQ=255)
                                                                               gcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGt                                                                     >  1:607891/1‑151 (MQ=255)
                                                                                   ggCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCg                                                                 <  1:1213242/151‑1 (MQ=255)
                                                                                   ggCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCg                                                                 <  1:936171/151‑1 (MQ=255)
                                                                                      cACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATc                                                              >  1:1025747/1‑151 (MQ=255)
                                                                                       aCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCt                                                             >  1:835975/1‑151 (MQ=255)
                                                                                       aCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCt                                                             <  2:842576/151‑1 (MQ=255)
                                                                                       aCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCt                                                             <  2:1085225/151‑1 (MQ=255)
                                                                                          cTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACGCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTgg                                                           <  2:510805/150‑1 (MQ=255)
                                                                                            cAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGt                                                          <  1:477551/149‑1 (MQ=255)
                                                                                             aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGTAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGAt                                                       <  2:801343/151‑1 (MQ=255)
                                                                                             aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGAt                                                       <  1:907351/151‑1 (MQ=255)
                                                                                             aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGAt                                                       <  2:463575/151‑1 (MQ=255)
                                                                                                aCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCct                                                    <  1:81180/151‑1 (MQ=255)
                                                                                                  aTTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCctgc                                                  >  2:651274/1‑151 (MQ=255)
                                                                                                     gATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                               >  1:383403/1‑151 (MQ=255)
                                                                                                     gATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                               >  2:597171/1‑151 (MQ=255)
                                                                                                      aTTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGga                                              <  2:1209383/151‑1 (MQ=255)
                                                                                                       ttttGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                               >  2:1083587/1‑149 (MQ=255)
                                                                                                       ttttGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                               >  2:955428/1‑149 (MQ=255)
                                                                                                        tttGCGGTGAAATATGCCAATAAAGCGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGGACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                               >  1:668316/1‑148 (MQ=255)
                                                                                                          tGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaac                                          >  2:573918/1‑151 (MQ=255)
                                                                                                           gCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaac                                          >  1:1098164/1‑150 (MQ=255)
                                                                                                           gCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTACAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaa                                           >  2:813280/1‑149 (MQ=255)
                                                                                                             ggTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaacaac                                       <  2:1178642/151‑1 (MQ=255)
                                                                                                             ggTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaacaac                                       >  1:599648/1‑151 (MQ=255)
                                                                                                             ggTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaacaac                                       <  2:1020302/151‑1 (MQ=255)
                                                                                                              gTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAacaaca                                      <  1:1259027/151‑1 (MQ=255)
                                                                                                               tGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAacaaca                                      >  2:196701/1‑150 (MQ=255)
                                                                                                               tGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAacac                                     >  2:474308/1‑151 (MQ=255)
                                                                                                                 aaaTATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACg                                    <  1:974731/150‑1 (MQ=255)
                                                                                                                     atGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtg                               <  2:689534/151‑1 (MQ=255)
                                                                                                                        ccAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgggt                            <  2:987208/151‑1 (MQ=255)
                                                                                                                             caaGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCgg                       >  1:905120/2‑151 (MQ=255)
                                                                                                                             aaaGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCgg                       <  1:926961/151‑1 (MQ=255)
                                                                                                                             aaaGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCgg                       >  2:490255/1‑151 (MQ=255)
                                                                                                                             aaaGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCg                        <  2:1080942/150‑1 (MQ=255)
                                                                                                                               aGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTCCGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGAt                     <  2:378215/151‑1 (MQ=255)
                                                                                                                               aGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGAt                     >  2:268221/1‑151 (MQ=255)
                                                                                                                               aGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGAt                     <  1:648175/151‑1 (MQ=255)
                                                                                                                                  ggCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGt                  >  2:328843/1‑151 (MQ=255)
                                                                                                                                   gCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGtt                 >  2:1262727/1‑151 (MQ=255)
                                                                                                                                   gCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGtt                 <  1:353695/151‑1 (MQ=255)
                                                                                                                                     aTTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                >  2:800916/1‑150 (MQ=255)
                                                                                                                                     aTTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                >  2:42212/1‑150 (MQ=255)
                                                                                                                                      tttATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTAc              >  2:573230/1‑151 (MQ=255)
                                                                                                                                         aTCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCa           <  1:879320/151‑1 (MQ=255)
                                                                                                                                         aTCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCa           <  1:1242389/151‑1 (MQ=255)
                                                                                                                                          tCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGc            >  1:913895/1‑149 (MQ=255)
                                                                                                                                           cGATACCGCTTTTGTCACCACTCAGGCGTTCTGCACAAAGTACGAATGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACTTCACGACGTGGGTGTCGGATGGgtt                >  1:745860/1‑144 (MQ=255)
                                                                                                                                           cGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAg          <  2:546548/150‑1 (MQ=255)
                                                                                                                                           cGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGc         <  2:1295551/151‑1 (MQ=255)
                                                                                                                                           cGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGc         >  1:836019/1‑151 (MQ=255)
                                                                                                                                                  gATTTTGACCCCACTCAGGCCTTCTCCAAAAAAGACGAAGTGCAGCACCATCCGTTCCTGCAGGCTCTCATTGGTCAATACCGTGTCGATCTTGTTATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGGTGGTTTACGCAGCTTCGCCa  <  1:265583/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCA  >  NC_000913/4628099‑4628395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: