| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,875 | 0 | A | T | 48.4% | 28.3 / 12.6 | 32 | N272K (AAT→AAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (12/4); new base T (0/15); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAT > NC_000913/357728‑358021 | gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTAt < 2:1067161/149‑1 (MQ=255) tccgCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTccg‑cc > 2:186984/4‑151 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTccg‑ccg < 2:884357/151‑1 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCAGCTACCGTGATTCATTT‑CCGCC‑AAAAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGcaac > 1:472883/1‑151 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGcaacaa < 1:532080/150‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TG‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑ACGCAACAAAAccgcc > 2:47916/1‑151 (MQ=255) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAAccgccg > 2:202169/1‑150 (MQ=255) aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCCCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTtctct > 2:160559/1‑151 (MQ=255) cAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCagt > 1:1132002/1‑150 (MQ=255) ccGCAGTCTTGTGGCAGCGGCTCCGGTAATTCATTC‑CCGCC‑ACCACCCGCACTTATACCCCGC‑CCTT‑GTTGAAAAATTCCTTT‑C‑GGGGCGTTTCCCTGCGTTTTTTTTTCCCG‑CCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCgg < 1:736265/149‑1 (MQ=255) agccttgtgTCGGCGCCTCCCGTTTTTCTTTC‑CCGCC‑AACACCCGCGTTTTTA‑CCCACCCCATGGTGAAAAAATGCC‑TC‑C‑GGGGGGGCTGTCTCCCAGTTTTTTTCCCG‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 1:400497/143‑1 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggtggcc > 2:362509/1‑145 (MQ=255) tgccggCGGCTCCGGTGATTCTTTC‑CCCCT‑AACACCCGCGCATTTACCCACCGCCAT‑GTGAAAAAATGCT‑TC‑C‑GCGGCGGCTGTCTGCCAGCTTTTTTCCCG‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAAt < 1:674863/145‑1 (MQ=255) tcggcggcgACCGT‑ATTCATTTCCCCCC‑ACCACCCGCTCTTTTCACCCGCGCAAG‑GTG‑AAAAATTCC‑TT‑CGGGGGGGGGTGTCCCCCACCT‑TTTTTCCGCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAg < 1:644299/147‑1 (MQ=255) aGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGCGG‑CAGAGaaaa > 1:38025/1‑145 (MQ=255) ttcttt‑CCCCCAAACACCCCCGCTTTTACCAACCCCCAG‑GTGAAAAAATCCC‑TC‑C‑GCGGCGGCGGTCTGCCCGTTTTTTTCCCG‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCAtg < 1:568129/147‑1 (MQ=255) ttcccccc‑AACACCCCCGCTTTTACCCACCCCCAG‑GTGAAAAAGTCCC‑TC‑C‑GGGGGGGCTTTCGCCAAGTTTTTTTCCCG‑CCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCAtgttg < 1:870330/143‑1 (MQ=255) cccccc‑AACACCCGCGTTTTAATCCACCCCAAT‑GTGAAAAAAGGCC‑TC‑C‑GGGGCGGCTTTCTGCCAGTTTTTTTCCCG‑CCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTtgtgt < 1:1080326/146‑1 (MQ=255) cccGCA‑AACACCCGCGATTTTACCCACCCCCAT‑GTAAAAAAATCCC‑TG‑C‑GGGGCGGCTGTCGCCCAGTTTTTTTCCCG‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCAtgtt < 1:550202/145‑1 (MQ=255) acaacCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGG‑GAGAGAAAAAACTTTTAAACCGTCCTGTTTTGTgggga > 1:521945/1‑145 (MQ=25) ccccgttttcccccACCGCAAT‑GTG‑AAAAATGCC‑TTCC‑GGGGGGCCGGTCTCCCGTTTTTTTTCCCG‑CCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 2:428878/138‑1 (MQ=255) tttcccaaccGCCAG‑GAGAAAAATTCCC‑TT‑C‑GGGGCGGTTGTCGGCCATTTTTTTTCCCG‑CCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTaa < 2:1131927/141‑1 (MQ=255) tttACCCCACGCCCT‑GCCAAAAACTGCT‑TC‑C‑GCGGCGGCTGTCGCCCATTTTTTTCCCCC‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:201607/149‑1 (MQ=255) ttATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGG‑CGGAGAAAAAGCCTTAAGAACCCCATGTTGTGTGGGaaacagcgctcgtaac > 1:1108577/1‑136 (MQ=25) aCGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGA‑GCAAGAAAAAAATTTATATCCCCCCTGTTGTGTGGGGGTCGCCGCTGGTGACAAtgt > 1:326037/1‑148 (MQ=21) aT‑GTGCAAAAATGCC‑TC‑C‑GCGGCGGCTGTCTGCCACTTTTTTTCCCC‑CCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 1:1174346/145‑1 (MQ=255) aaaaaTCCC‑TT‑C‑CCGGCGGCTCTCTCCCATTTTTTTTCCCC‑CCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTTGTAACAATGTGGAAAGGGAAGTgc < 1:975767/151‑1 (MQ=255) gcggcgGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAAGGGAAAAAAATTAAAAGCCGACCGGTTTTGGGGAATTCCCTCTGGGGAACAAGGTGGAAAGGGaaaggggggaaggcg > 2:528509/1‑136 (MQ=255) gcgtcTGCCTGCCGGTTTTTTCGCCG‑CCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTc < 2:233374/149‑1 (MQ=255) tGTCTCCCACCTTTTTTCCCC‑CCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa < 1:63934/149‑1 (MQ=255) ccAGTTTTTTTCCCC‑CCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg < 1:1035921/146‑1 (MQ=255) acttATTTTCCCG‑CCCAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt < 1:281345/148‑1 (MQ=255) | GAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCC‑AACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGC‑AAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAT > NC_000913/357728‑358021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |