Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 430,137 T→G L211L (CTT→CTG secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913430,1370TG98.8% 303.5 / NA 83L211L (CTT→CTGsecFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (44/38);  minor base C (1/0);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTATCCTG  >  NC_000913/430006‑430284
                                                                                                                                   |                                                                                                                                                   
cGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTAt                                                                                                                                  <  1:889866/151‑1 (MQ=255)
  aCTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGTTGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATc                                                                                                                                 >  2:1148614/1‑150 (MQ=255)
  aCTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCg                                                                                                                                >  1:201454/1‑151 (MQ=255)
    tGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCgg                                                                                                                               >  2:874317/1‑150 (MQ=255)
      gcggcAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGAcc                                                                                                                            >  2:567606/1‑151 (MQ=255)
         gcAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTa                                                                                                                         <  2:322539/151‑1 (MQ=255)
          cAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATc                                                                                                                                 >  1:592760/1‑142 (MQ=255)
          cAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATc                                                                                                                                 <  2:592731/142‑1 (MQ=255)
             gggTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCg                                                                                                                     <  1:1090699/151‑1 (MQ=255)
               gtggtTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTg                                                                                                                   >  1:293802/1‑151 (MQ=255)
                 ggtTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGa                                                                                                                  >  2:90634/1‑150 (MQ=255)
                 ggtTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGa                                                                                                                  >  2:246214/1‑150 (MQ=255)
                   ttATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGaaa                                                                                                                >  1:1055417/1‑150 (MQ=255)
                    taatGCGCTGGCGCACGACGTTACCATTACGCCGGGTATTTTGCCGTTCTTCCATATCGCGATTGACCTGACCCTTGTGGCATCGTTGATGTCGGTTATCCGTTACTCGCTGAACGACAGTACCGTGGTATCGGACCGTATTCGTGACAAc                                                                                                              >  2:480812/4‑151 (MQ=255)
                    tATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGaaaa                                                                                                               <  2:802758/150‑1 (MQ=255)
                    tATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGaaaa                                                                                                               <  1:866723/150‑1 (MQ=255)
                    tATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGaaaa                                                                                                               <  2:418665/150‑1 (MQ=255)
                        gcgcTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTc                                                                                                           <  1:388404/150‑1 (MQ=255)
                           cTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGc                                                                                                        >  1:744228/1‑150 (MQ=255)
                            tGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCa                                                                                                       >  1:849377/1‑150 (MQ=255)
                             ggCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCaa                                                                                                      <  1:294461/150‑1 (MQ=255)
                             ggCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAg                                                                                                     <  1:278115/151‑1 (MQ=255)
                             ggCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAg                                                                                                     <  2:676740/151‑1 (MQ=255)
                               cgcACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCa                                                                                                       >  2:453493/1‑147 (MQ=255)
                                 cACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATcc                                                                                                 <  1:209474/151‑1 (MQ=255)
                                   cgacgTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCg                                                                                                >  2:970721/1‑150 (MQ=255)
                                    gacgTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTc                                                                                              <  2:1158220/151‑1 (MQ=255)
                                    gacgTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTc                                                                                              <  1:109227/151‑1 (MQ=255)
                                        ttATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTcgc                                                                                            <  2:510506/149‑1 (MQ=255)
                                        ttATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCgg                                                                                          >  2:951431/1‑151 (MQ=255)
                                          aTCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGt                                                                                         >  2:533845/1‑150 (MQ=255)
                                          aTCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGt                                                                                         >  1:1042264/1‑150 (MQ=255)
                                          aTCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTa                                                                                        <  1:1127694/151‑1 (MQ=255)
                                            cATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACg                                                                                      <  1:1039008/151‑1 (MQ=255)
                                            cATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGGGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGt                                                                                         >  2:688023/1‑148 (MQ=255)
                                             aTTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACg                                                                                      <  1:955882/150‑1 (MQ=255)
                                                 cGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTa                                                                                 <  2:349774/151‑1 (MQ=255)
                                                        tATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATc                                                                          >  2:383350/1‑151 (MQ=255)
                                                         aTTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATc                                                                          <  1:713568/150‑1 (MQ=255)
                                                            ttGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCttt                                                                       >  1:386600/1‑150 (MQ=255)
                                                                   tATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTGCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACgtgt                                                                <  1:618516/150‑1 (MQ=255)
                                                                    aTTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTcc                                                              >  1:633408/1‑151 (MQ=255)
                                                                    aTTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTc                                                               <  1:572702/150‑1 (MQ=255)
                                                                       ccATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCtt                                                            <  2:1082619/150‑1 (MQ=255)
                                                                       ccATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTg                                                           >  2:807660/1‑151 (MQ=255)
                                                                       ccATATCGAGATTGAACTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCtt                                                            <  2:746725/150‑1 (MQ=255)
                                                                         atatCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAc                                                         >  2:587519/1‑151 (MQ=255)
                                                                         atatCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAc                                                         >  1:1089337/1‑151 (MQ=255)
                                                                           atCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAccc                                                       >  1:1043683/1‑151 (MQ=255)
                                                                           atCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGGATTCGTGAAAACTTGCGCAAGATCCGTCTCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGa                                                          >  2:1095911/1‑148 (MQ=255)
                                                                             cGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAg                                                     <  1:633538/151‑1 (MQ=255)
                                                                              gagaTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAcc                                                        >  2:851213/1‑147 (MQ=255)
                                                                               agaTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGAc                                                   <  2:925713/151‑1 (MQ=255)
                                                                               agaTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGGACGCCTTACGAAATCTTTAACGGGTCCTTGACCCAGa                                                    >  1:1073727/1‑150 (MQ=255)
                                                                                gaTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACg                                                  <  1:780017/151‑1 (MQ=255)
                                                                                gaTTGACCTGACCATTGTGGCATCGTTGATGCCGGTTATCGGGTACTCGCTCAACGACATTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCGTACGAAATCTTTAACGAGTCCTTGACCCAGACg                                                  >  2:170609/1‑151 (MQ=255)
                                                                                 aTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGc                                                 >  1:983694/1‑151 (MQ=255)
                                                                                 aTTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGc                                                 >  2:925386/1‑151 (MQ=255)
                                                                                   tgacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTg                                               >  1:254467/1‑151 (MQ=255)
                                                                                   tgacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTg                                               >  1:1185535/1‑151 (MQ=255)
                                                                                         gaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGt                                         >  1:811662/1‑151 (MQ=255)
                                                                                            cATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTAcc                                      >  1:401507/1‑151 (MQ=255)
                                                                                             aTTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCt                                     >  1:637162/1‑151 (MQ=255)
                                                                                             aTTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCt                                     >  1:496765/1‑151 (MQ=255)
                                                                                               tgtgGCATCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCtt                                    <  2:597455/150‑1 (MQ=255)
                                                                                                     aTCGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATcac                              >  2:566265/1‑150 (MQ=255)
                                                                                                       cGTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATc                           <  1:188176/151‑1 (MQ=255)
                                                                                                        gTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATcc                          <  1:652064/151‑1 (MQ=255)
                                                                                                        gTTGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATcc                          <  1:1130348/151‑1 (MQ=255)
                                                                                                         ttGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATcc                          <  2:709463/150‑1 (MQ=255)
                                                                                                          tGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTCCCTTGATCACATCCg                         <  2:841256/150‑1 (MQ=255)
                                                                                                          tGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCgg                        >  2:392885/1‑151 (MQ=255)
                                                                                                          tGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCgg                        >  1:613991/1‑151 (MQ=255)
                                                                                                          tGATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCg                         <  1:728148/150‑1 (MQ=255)
                                                                                                           gATGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCgg                        >  2:982475/1‑150 (MQ=255)
                                                                                                            aTGTCGGTTATCGGTTACTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCTCATCCGGta                      <  2:430780/151‑1 (MQ=255)
                                                                                                                       cggttaCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGAt            <  1:807702/150‑1 (MQ=255)
                                                                                                                        ggttaCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATgg          <  1:676537/151‑1 (MQ=255)
                                                                                                                           taCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGtt        >  1:502103/1‑150 (MQ=255)
                                                                                                                            aCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTAt      >  1:17559/1‑151 (MQ=255)
                                                                                                                            aCTCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTAt      >  1:540789/1‑151 (MQ=255)
                                                                                                                              tCGCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTATcc    >  2:1134304/1‑151 (MQ=255)
                                                                                                                                gCTGAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTATCCTg  <  1:1126184/151‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                   
CGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTATCCTG  >  NC_000913/430006‑430284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: