| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 622,725 | A→C | E142D (GAA→GAC) | entS → | enterobactin exporter, iron‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 622,725 | 0 | A | C | 98.3% | 206.1 / ‑3.3 | 60 | E142D (GAA→GAC) | entS | enterobactin exporter, iron‑regulated |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (33/25); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
ATTGGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACC > NC_000913/622579‑622866 | aTTGGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACt > 2:495979/1‑151 (MQ=255)aTTGGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACt > 1:451200/1‑151 (MQ=255) gCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAAt < 1:1115881/151‑1 (MQ=255) cTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAAt > 2:1131014/1‑150 (MQ=255) ttCTTTGGACTGGGCCTTAATGCACTGCTGCCGGAGCCGTCAAGGCTGGCAATCTATTTCCTTGGTTTATGGGATGGTTTTTTCGCCTAGCTTGGCGTTACGGCGCTATTGGCCTCGACACCCGCCATGTTCGGGCGTGACGACTTATTgt > 2:716266/1‑150 (MQ=255) tCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATg < 2:1130795/149‑1 (MQ=255) aTTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAgg < 1:883733/151‑1 (MQ=255) gtgCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGc < 1:987940/150‑1 (MQ=255) gtgCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCg < 2:66288/151‑1 (MQ=255) ccTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATc > 1:771617/1‑151 (MQ=255) aaTGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCAcc > 1:951447/1‑150 (MQ=255) gCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGt > 2:1006810/1‑151 (MQ=255) gCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGt > 2:202221/1‑151 (MQ=255) tgcCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGt > 1:939306/1‑151 (MQ=255) ccGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGc < 2:852283/151‑1 (MQ=255) ggAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCg > 2:312548/1‑150 (MQ=255) ggAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGt < 2:743579/151‑1 (MQ=255) ggAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGt > 1:971106/1‑151 (MQ=255) gAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTATCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGTGCGATCACCATGTTGGCCGTGCg < 2:827298/149‑1 (MQ=255) cGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTggg > 1:908841/1‑151 (MQ=255) cGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTggg < 1:838792/151‑1 (MQ=255) tCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTgggg > 1:1113392/1‑150 (MQ=255) tCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGt > 1:368937/1‑151 (MQ=255) cATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTc < 2:908780/151‑1 (MQ=255) cAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGAttt > 2:785044/1‑150 (MQ=255) tttACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATg > 1:695617/1‑151 (MQ=255) tttACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATg > 1:733950/1‑151 (MQ=255) ttACTTGGTTTATGGGATGGTTTTTTCGCATCCCTTGGCGTTACGGCGCTATTGGCGGCGACACCCGCACTGGGAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCCGCCATg > 2:862867/1‑150 (MQ=255) aCTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAtt > 2:101083/1‑151 (MQ=255) aCTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAtt < 1:123685/151‑1 (MQ=255) tGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTgg < 2:344926/150‑1 (MQ=255) tttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCgg > 1:216976/1‑150 (MQ=255) gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGGTTAtt > 2:178737/1‑151 (MQ=255) gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGATAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAtt < 1:196592/151‑1 (MQ=255) ggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTg < 2:290184/151‑1 (MQ=255) aTGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGAGACACCAGCACTGGGAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGAACGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGc > 1:58118/1‑150 (MQ=255) ggTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTgg < 2:1053214/151‑1 (MQ=255) ttCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGAcc < 1:75898/150‑1 (MQ=255) tCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGAcc < 2:451795/149‑1 (MQ=255) cGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGt < 2:1177461/151‑1 (MQ=255) cGCATCGCTTGGCGTTACGGAGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCg > 2:553083/1‑149 (MQ=255) cATCGCTTGGCGTTACGGCGCTATTGGCGGCGAACCCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTGGCCCATGCTTGGCGGTTTTCTGCCAGCGGCCGgggg > 2:951120/1‑151 (MQ=255) aTCGCTTGGCGTTACGGCGCTATTGGCGGCTACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGc < 1:671662/151‑1 (MQ=255) aTCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAGAACTTAATGCAGGCCGGGGCGCTCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTTTTGCCGGCGACCCGTgg > 1:1125921/1‑150 (MQ=255) aTCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGc > 1:1091546/1‑151 (MQ=255) gCTTGGCGCTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGGGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGAACGGGGGCGt > 2:1186643/1‑150 (MQ=255) cTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCg > 1:877992/1‑148 (MQ=255) ggCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCt < 1:1161206/151‑1 (MQ=255) cgcTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACgg < 2:942502/151‑1 (MQ=255) gcTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACggg < 2:708181/151‑1 (MQ=255) cTATTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACggg > 2:732593/1‑150 (MQ=255) aTTGGCGGCGACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCt < 1:239569/150‑1 (MQ=255) cggcgACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcggc > 1:735297/1‑151 (MQ=255) gACACCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCTCCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTTCGGGCTGgcggcggcg < 2:956114/151‑1 (MQ=255) cacCAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCggg > 1:735955/1‑151 (MQ=255) cAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCggggcc > 2:886888/1‑148 (MQ=255) cAGCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCAc > 1:932083/1‑151 (MQ=255) gCACTGGTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGt > 2:491473/1‑151 (MQ=255) ggTAGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGGGGCGGAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatt > 2:189333/1‑151 (MQ=255) aGGGCGTGACAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTAcc < 1:319868/151‑1 (MQ=255) | ATTGGCTTCATTGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACC > NC_000913/622579‑622866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |