Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,664	A→C	D41E (GAT→GAG)	yaaI ←	UPF0412 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,664	0	A	C	97.5%	282.2 / ‑1.2	80	D41E (GAT→GAG)	yaaI	UPF0412 family protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (38/40);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

TATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGT  >  NC_000913/11520‑11811                                                                                                                                                 |                                                                                                                                                    tATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGtt                                                                                                                                                <  1:271906/150‑1 (MQ=255)   tttCTGAAGGAATCTTCAGGCTCTAACTGGCGCTACGGGTGGAGTTGAAATAAACCGATGCAGCGCTTATCTGTAAATCGCCATGGGCGGGATACATTTGTATGCGTTTCACAATGCTATAGCCAGGAAGGTTGAGCGACAGCttgttg                                                                                                                                               <  2:627557/149‑1 (MQ=255)    ttCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGt                                                                                                                                             <  2:318570/150‑1 (MQ=255)     tCTGAAGGAATATTCAGGCTCTGGCCGGCGGTACGGGCGGGTTTGAAATAAACCGGTGCCACGCCTTACTGTAAATCGACATGGTCGGGAGAGAGTGATATGCGTTTCACAATGCGGCAAACATGAAGTTTCAGCGCCAGCTCGTTg                                                                                                                                               <  2:131467/147‑1 (MQ=255)       tGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCg                                                                                                                                         >  1:87585/1‑151 (MQ=255)         aaGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTACATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGtt                                                                                                                                       <  1:586825/151‑1 (MQ=255)          aGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGtt                                                                                                                                       >  1:108947/1‑150 (MQ=255)           ggAATATTCAGGCTCTGACTGGCGCTACGGTCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAc                                                                                                                                     <  1:667844/151‑1 (MQ=255)            gAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAcg                                                                                                                                    >  2:104206/1‑151 (MQ=255)             aaTATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAcgc                                                                                                                                   >  2:28160/1‑151 (MQ=255)             aaTATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAcgc                                                                                                                                   >  1:476143/1‑151 (MQ=255)              atatTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTAcgcg                                                                                                                                  >  1:368488/1‑151 (MQ=255)                 ttCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGc                                                                                                                                >  1:458192/1‑150 (MQ=255)                  tCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCAt                                                                                                                              >  1:243186/1‑151 (MQ=255)                     ggCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGc                                                                                                                           <  1:392457/151‑1 (MQ=255)                       ctctGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCa                                                                                                                          >  1:785855/1‑150 (MQ=255)                           gACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATg                                                                                                                       <  1:1006768/149‑1 (MQ=255)                                gcgcTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCg                                                                                                                 >  1:110215/1‑150 (MQ=255)                                     aCGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCgagga                                                                                                             <  1:549277/149‑1 (MQ=255)                                       gggCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGttt                                                                                                         >  2:474655/1‑151 (MQ=255)                                        ggcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGttt                                                                                                         >  1:1022250/1‑150 (MQ=255)                                         gcggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTa                                                                                                        <  2:779212/150‑1 (MQ=255)                                           ggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATgg                                                                                                     >  2:79266/1‑151 (MQ=255)                                           ggcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATgg                                                                                                     >  2:26107/1‑151 (MQ=255)                                            gcTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGt                                                                                                    <  1:857572/151‑1 (MQ=255)                                             cTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTc                                                                                                   >  1:483645/1‑151 (MQ=255)                                                tGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGtt                                                                                                >  2:902330/1‑151 (MQ=255)                                                 gAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt                                                                                               >  2:849324/1‑151 (MQ=255)                                                   aaTAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGc                                                                                             >  1:576894/1‑151 (MQ=255)                                                    aTAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGcc                                                                                            >  1:950541/1‑151 (MQ=255)                                                      aaaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCt                                                                                           <  1:186252/150‑1 (MQ=255)                                                      aaaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTg                                                                                          >  1:729323/1‑151 (MQ=255)                                                          cGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGcc                                                                                      >  2:641504/1‑151 (MQ=255)                                                              gCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgc                                                                                  >  1:593656/1‑151 (MQ=255)                                                               cACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTgca                                                                                 <  2:834810/151‑1 (MQ=255)                                                                    cTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcac                                                                             >  2:52240/1‑150 (MQ=255)                                                                      tAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcaca                                                                            <  2:309176/149‑1 (MQ=255)                                                                       aaCCGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGcagcaca                                                                            >  2:390374/1‑148 (MQ=255)                                                                          tGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATc                                                                       >  2:497635/1‑150 (MQ=255)                                                                          tGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGACGTTTGCCTGCGCCGTGCAGCACAGCAt                                                                        >  1:662801/1‑149 (MQ=255)                                                                           gTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAg                                                                     <  2:535436/151‑1 (MQ=255)                                                                             aaaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGc                                                                   <  1:833360/151‑1 (MQ=255)                                                                              aaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCt                                                                  >  2:1142861/1‑151 (MQ=255)                                                                              aaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCt                                                                  <  2:610334/151‑1 (MQ=255)                                                                              aaTCGCCATGGCCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCt                                                                  >  2:569303/1‑151 (MQ=255)                                                                               aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCt                                                                  >  2:1174070/1‑150 (MQ=255)                                                                                  gCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATc                                                              <  2:722765/151‑1 (MQ=255)                                                                                   ccATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCg                                                             <  2:1078265/151‑1 (MQ=255)                                                                                       ggTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAg                                                         <  2:536519/151‑1 (MQ=255)                                                                                       ggTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAg                                                         <  1:1134155/151‑1 (MQ=255)                                                                                        gTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAgg                                                        >  2:451385/1‑151 (MQ=255)                                                                                          cGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCt                                                      <  1:1194152/151‑1 (MQ=255)                                                                                          cGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCt                                                      <  1:1051500/151‑1 (MQ=255)                                                                                           ggCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTg                                                     >  2:1023877/1‑151 (MQ=255)                                                                                            gCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTgg                                                    <  1:150239/151‑1 (MQ=255)                                                                                             cAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGc                                                   <  2:978004/151‑1 (MQ=255)                                                                                             cAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGc                                                   <  1:851991/151‑1 (MQ=255)                                                                                               gagagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCgg                                                 <  2:518322/151‑1 (MQ=255)                                                                                                    ttGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGGGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATc                                            >  2:192007/1‑151 (MQ=255)                                                                                                    ttGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATc                                            >  2:1003169/1‑151 (MQ=255)                                                                                                     tGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATc                                            <  1:1171941/150‑1 (MQ=255)                                                                                                        aTGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTa                                         <  2:883597/150‑1 (MQ=255)                                                                                                          gCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaaa                                      <  2:436998/151‑1 (MQ=255)                                                                                                                cacaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAt                                >  2:553611/1‑151 (MQ=255)                                                                                                                cacaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAt                                <  2:1022181/151‑1 (MQ=255)                                                                                                                cacaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAt                                <  2:492651/151‑1 (MQ=255)                                                                                                                cacaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAt                                >  2:182361/1‑151 (MQ=255)                                                                                                                 acaATGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAtt                               <  2:1148977/151‑1 (MQ=255)                                                                                                                   aaTGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAttt                              >  2:408302/1‑150 (MQ=255)                                                                                                                    aTGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTc                             >  1:521569/1‑150 (MQ=255)                                                                                                                    aTGCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCa                            <  1:846931/151‑1 (MQ=255)                                                                                                                      gCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCAt                           <  1:739646/150‑1 (MQ=255)                                                                                                                      gCGACAAACAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCAt                           <  1:641837/150‑1 (MQ=255)                                                                                                                            aaCAGGAAGTTTCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGAtt                    <  1:864965/151‑1 (MQ=255)                                                                                                                                      ttCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg          >  2:292006/1‑151 (MQ=255)                                                                                                                                       tCAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg          <  1:719971/150‑1 (MQ=255)                                                                                                                                        cAGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTggg         >  1:433785/1‑150 (MQ=255)                                                                                                                                         aGCGCCAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa       <  1:611011/151‑1 (MQ=255)                                                                                                                                              cAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGt  <  2:476120/151‑1 (MQ=255)                                                                                                                                              cAGCTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGt  >  2:265037/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGT  >  NC_000913/11520‑11811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑