Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,113,464 T→G intergenic (+58/‑115) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,4640TG100.0% 313.5 / NA 81intergenic (+58/‑115)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/43);  total (38/43)

CCGGAGAGATATTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTG  >  NC_000913/1113317‑1113608
                                                                                                                                                   |                                                                                                                                                 
ccGGAGAGATATTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGAt                                                                                                                                                >  1:598779/1‑151 (MQ=255)
    agagaTATTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTg                                                                                                                                            >  2:160320/1‑151 (MQ=255)
      agaTATTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCa                                                                                                                                          <  2:14496/151‑1 (MQ=255)
        atatTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCa                                                                                                                                          >  1:817610/1‑149 (MQ=255)
          atTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGa                                                                                                                                       <  2:668760/150‑1 (MQ=255)
                  tGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTggg                                                                                                                             >  1:1191343/1‑151 (MQ=255)
                   gggTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGt                                                                                                                            <  1:905847/151‑1 (MQ=255)
                     gTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCg                                                                                                                          >  2:528508/1‑151 (MQ=255)
                      tGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCgg                                                                                                                         >  1:308440/1‑151 (MQ=255)
                       gAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCgg                                                                                                                         >  2:200916/1‑150 (MQ=255)
                         gTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGAt                                                                                                                       >  2:1199057/1‑150 (MQ=255)
                             ttaCGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGATTTTGTGGGTCGGATaa                                                                                                                     <  1:969787/148‑1 (MQ=255)
                                 gAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTc                                                                                                              <  1:66518/151‑1 (MQ=255)
                                   aGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCAc                                                                                                            >  1:135134/1‑151 (MQ=255)
                                    gggATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCAcg                                                                                                           <  1:1077404/151‑1 (MQ=255)
                                       aTAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCAcgccg                                                                                                        >  1:252591/1‑151 (MQ=255)
                                           aGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATc                                                                                                    <  2:466909/151‑1 (MQ=255)
                                             cTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATcc                                                                                                   <  2:140969/150‑1 (MQ=255)
                                                aaTCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                >  1:267815/1‑150 (MQ=255)
                                                   ccACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCaaa                                                                                             >  1:353992/1‑150 (MQ=255)
                                                    cACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCaaaa                                                                                            >  1:99252/1‑150 (MQ=255)
                                                     aCTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaac                                                                                          >  1:160822/1‑151 (MQ=255)
                                                      cTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaac                                                                                          <  1:1076580/150‑1 (MQ=255)
                                                        ggCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac                                                                                       <  1:97130/151‑1 (MQ=255)
                                                        ggCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaaca                                                                                         <  1:854183/149‑1 (MQ=255)
                                                        ggCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaaca                                                                                         <  2:547266/149‑1 (MQ=255)
                                                           aTTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACg                                                                                      >  2:283902/1‑149 (MQ=255)
                                                           aTTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACg                                                                                      >  2:437512/1‑149 (MQ=255)
                                                           aTTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACg                                                                                      >  2:593267/1‑149 (MQ=255)
                                                                   aaCCGTAGGCGGCTTCGCAATAAAAACGTGTTTGCCGGATGCGCTTCGTTTATCGGTCGTAATTCGTTCCTGCAATTAATGGATTTTGCAAGACTTTGTGGGTGGGATAAGGGGTGCACGCCGCATCCGGCAAAAACAACGAGCCAATaaa                                                                            <  1:161650/151‑1 (MQ=255)
                                                                       ggATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATa                                                                        <  1:988030/151‑1 (MQ=255)
                                                                       gaatggatcTTCGAAAAAAAATCGAAGTTGCGGTATGCCCCACGCTTTTCAGGCCAAATCCTTTCCTGCAGTTAATGTAATTTGCAAGACTTTGTGTGTCAGAAAAGACGTTCACTCCGCATCCGGCAAAAACAACGAGCCAATaaaa                                                                           <  2:411527/140‑1 (MQ=255)
                                                                        gATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATAc                                                                       >  1:702815/1‑151 (MQ=255)
                                                                             ggCTTCGCAATAAAAACGTATTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTGGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCg                                                                     <  2:198013/148‑1 (MQ=255)
                                                                             ggCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGTTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCg                                                                     <  2:831526/148‑1 (MQ=255)
                                                                             ggCTTCGCAAAAAAAACGTAGTTGCCGGATGCGCTGCTCTTATCGGGCCTACATTGTCCCGCCAATTTTGGAAGTTGGAAAACCTTTGGGGGGCGGAAAAGGGGTTCCCGCCGCAACCGGAAAAACCAACGAGCCAATAAAAATACCGGc                                                                   <  2:1108604/150‑1 (MQ=255)
                                                                               cTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCg                                                                  <  2:450139/149‑1 (MQ=255)
                                                                               cTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                >  2:320678/1‑151 (MQ=255)
                                                                               cTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                >  2:899583/1‑151 (MQ=255)
                                                                                ttCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTGGGATAAGGCTTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCg                                                                  <  1:118089/148‑1 (MQ=255)
                                                                                ttCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                >  1:1019432/1‑150 (MQ=255)
                                                                                 tCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                <  1:931116/149‑1 (MQ=255)
                                                                                  cGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATg                                                             >  2:625846/1‑151 (MQ=255)
                                                                                  cGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGAAATTTATGGATTTTGAAAGACTTTGTGGGTCGAAAAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                <  2:646048/148‑1 (MQ=255)
                                                                                   gCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                            >  1:30306/1‑151 (MQ=255)
                                                                                       tAAAAACGTAGTTGCTTGATGCGCTACGCTTATCAGGCTTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                         <  1:1210480/150‑1 (MQ=255)
                                                                                        aaaaaCGTAGTTCCCTGAGGCGCTACCCTTATCAGGCATACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGAAAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                       <  2:233973/151‑1 (MQ=37)
                                                                                         aaaaCGTAGTTGCCTGTGGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGATTTTGTGGGTCGGATAAGGCTTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAt                                                      <  2:476562/151‑1 (MQ=255)
                                                                                         aaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAt                                                      >  2:910811/1‑151 (MQ=255)
                                                                                          aaaCGTGGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCTTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtt                                                     <  2:812795/151‑1 (MQ=255)
                                                                                            aCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTCCGTCTTTCCTGCAATTTAGGGATTTTGCACGACTTTGTGGGTGGAATAGGGCATTCACGCCGCATCGGGCAAAAACACCGAGCCAATAAAAATACCGGCGTTATGCCGGTAttt                                                    <  1:1011084/150‑1 (MQ=25)
                                                                                             cGTATTGGCCTGATGCACTCCGCTTATCAGGCATACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTGGTGGGTGGGATAAGGCGTTCACGCGGCAACCGGCAAAAAAAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttt                                                   <  1:395996/150‑1 (MQ=255)
                                                                                                aGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttttt                                                >  1:902025/1‑150 (MQ=255)
                                                                                                  ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGATTTTGTGGGTCGGATAGGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                               <  1:273056/149‑1 (MQ=255)
                                                                                                  ttGCCTGATGCCCTCCGCTTATCAGGCTTACTTCTTTCCTGCATTTTATGGATTTTGCAAGCCTTTGTGGGTCGGATAAGGCTTTCCCGCCGCATCCGTCAAAAACACCGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                              <  2:782534/150‑1 (MQ=255)
                                                                                                     ccTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaaa                                          <  1:476175/151‑1 (MQ=255)
                                                                                                      cTGATGCGCTACGCTTCTCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaa                                           >  1:1032369/1‑149 (MQ=255)
                                                                                                       tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaa                                           >  1:921927/1‑148 (MQ=255)
                                                                                                       tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaa                                           >  2:424684/1‑148 (MQ=255)
                                                                                                       tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCAGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaa                                           >  1:300759/1‑148 (MQ=255)
                                                                                                        gATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAaga                                        <  2:168383/150‑1 (MQ=255)
                                                                                                          tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGAAAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                       <  2:763051/149‑1 (MQ=255)
                                                                                                          tGCCCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                       <  2:262873/149‑1 (MQ=255)
                                                                                                           gcgcTACGCTTATCAGGCCTACATGGTTCCTGCAATTTATGGATTTTGAAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTa                                    <  1:593296/151‑1 (MQ=255)
                                                                                                           gcgcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAgg                                      <  1:62505/149‑1 (MQ=255)
                                                                                                            cgcTCCGCTTATCAGGCTTACAGCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGt                                     <  2:165462/149‑1 (MQ=255)
                                                                                                             gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                  >  1:88258/1‑151 (MQ=255)
                                                                                                              cTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                  >  2:1159293/1‑150 (MQ=255)
                                                                                                              cTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                  >  1:406468/1‑150 (MQ=255)
                                                                                                                aCGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCaa                                >  2:474164/1‑150 (MQ=255)
                                                                                                                 cGCTTATCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAAt                              >  2:808307/1‑151 (MQ=255)
                                                                                                                    ttATCAGGCCTACATCGTTCCTGCAATTTAGGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                             <  2:753340/149‑1 (MQ=255)
                                                                                                                      aTCAGGCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                         >  1:623954/1‑151 (MQ=255)
                                                                                                                          ggCCTACATCGTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAAt                     >  1:554779/1‑151 (MQ=255)
                                                                                                                                 aTCGTTCCTGCAATTTAGGGATTTTGAAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg              <  1:625876/151‑1 (MQ=255)
                                                                                                                                    gTTCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGc           >  1:292971/1‑151 (MQ=255)
                                                                                                                                     ttCCTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGc           <  1:1199134/150‑1 (MQ=255)
                                                                                                                                       ccTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc        <  1:43871/151‑1 (MQ=255)
                                                                                                                                       ccTGCAATTTATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc        <  2:252581/151‑1 (MQ=255)
                                                                                                                                              tttATGGTTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGACAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTg  <  1:200908/150‑1 (MQ=255)
                                                                                                                                              tttATGGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTg  >  2:740847/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGGAGAGATATTCTTCATGGGTGAGTTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTG  >  NC_000913/1113317‑1113608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: