Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,050	T→G	intergenic (+221/‑74)	cysB → / → ymiA	N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,050	0	T	G	100.0%	322.2 / NA	84	intergenic (+221/‑74)	cysB/ymiA	N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/44);  total (40/44)

TATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTG  >  NC_000913/1334902‑1335196                                                                                                                                                     |                                                                                                                                                   tataCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCg                                                                                                                                                  >  2:931955/1‑151 (MQ=255)  ataCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATgcgc                                                                                                                                                 >  2:969574/1‑151 (MQ=255)    aCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCt                                                                                                                                                <  1:1019442/150‑1 (MQ=255)    aCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACGCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCt                                                                                                                                                <  1:929698/150‑1 (MQ=255)       gTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAt                                                                                                                                            <  1:1160415/151‑1 (MQ=255)       gTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAt                                                                                                                                            <  2:544842/151‑1 (MQ=255)         gCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAtt                                                                                                                                           <  2:748700/150‑1 (MQ=255)          cacaATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAtttt                                                                                                                                         >  2:982186/1‑151 (MQ=255)           acaATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAttttt                                                                                                                                        <  1:603813/151‑1 (MQ=255)            caATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGAttttt                                                                                                                                        <  1:1100032/150‑1 (MQ=255)              atatTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGAc                                                                                                                                     <  2:949067/151‑1 (MQ=255)              atatTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGAc                                                                                                                                     >  2:702210/1‑151 (MQ=255)               tatTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACt                                                                                                                                    >  1:1123864/1‑151 (MQ=255)                atTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTTTAATTCCCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACt                                                                                                                                    <  1:639565/150‑1 (MQ=255)                atTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGAc                                                                                                                                     >  1:90020/1‑149 (MQ=255)                atTTTTATCCCTCATATTTATAGGGTAAACTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACt                                                                                                                                    <  1:667685/150‑1 (MQ=255)                 tttttATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTa                                                                                                                                   >  2:990158/1‑150 (MQ=255)                      aTCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGttt                                                                                                                             <  2:691159/151‑1 (MQ=255)                            cATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCttt                                                                                                                         <  1:738623/149‑1 (MQ=255)                            cATATTTATAGGGTAAATTACCTGTAAAGTAGTATCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCttt                                                                                                                         <  1:874792/149‑1 (MQ=255)                             atatTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCtt                                                                                                                          <  1:83258/147‑1 (MQ=255)                               atTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGa                                                                                                                     >  1:287242/1‑150 (MQ=255)                                tttATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGAtt                                                                                                                   >  2:303205/1‑151 (MQ=255)                                  tataGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTg                                                                                                                 >  1:879901/1‑151 (MQ=255)                                         tAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATc                                                                                                          >  1:861908/1‑151 (MQ=255)                                          aaaTTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATc                                                                                                          <  1:302575/150‑1 (MQ=255)                                           aaTTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGCCTAAAGTTTCTTTAGGATTTGTCTCATCGt                                                                                                        >  1:673053/1‑151 (MQ=255)                                           aaTTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGt                                                                                                        >  1:742494/1‑151 (MQ=255)                                           aaTTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGt                                                                                                        <  2:636162/151‑1 (MQ=255)                                            aTTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTa                                                                                                       <  1:405675/151‑1 (MQ=255)                                             ttACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTCGTCTCATCGTAt                                                                                                      >  2:1044288/1‑151 (MQ=255)                                               aCCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATg                                                                                                     <  1:1064773/150‑1 (MQ=255)                                                   gTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGatt                                                                                                  <  2:496366/149‑1 (MQ=255)                                                     aaaGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGattaat                                                                                               <  2:496488/150‑1 (MQ=255)                                                       agtagtGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTac                                                                                            <  1:1113028/151‑1 (MQ=255)                                                          agtGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTacac                                                                                          <  1:764944/150‑1 (MQ=255)                                                          agtGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTaca                                                                                           <  1:890634/149‑1 (MQ=255)                                                           gtgtCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCa                                                                                        <  2:912889/151‑1 (MQ=255)                                                            tgtCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTTAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGAATTTGGCTCATCTTATGATTAATTACACCa                                                                                        <  2:245918/150‑1 (MQ=255)                                                            tgtCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCa                                                                                        >  2:1057635/1‑150 (MQ=255)                                                             gtCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCaa                                                                                       <  2:315263/150‑1 (MQ=255)                                                              tCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATc                                                                                     >  1:370708/1‑151 (MQ=255)                                                                    tACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCa                                                                                >  2:845237/1‑150 (MQ=255)                                                                      cccGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACg                                                                             <  2:227451/151‑1 (MQ=255)                                                                           cTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTg                                                                        >  1:817456/1‑151 (MQ=255)                                                                           cTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTg                                                                        >  1:788791/1‑151 (MQ=255)                                                                              gCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTg                                                                     <  2:899077/151‑1 (MQ=255)                                                                              gCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTg                                                                     >  1:1015303/1‑151 (MQ=255)                                                                                 aaCTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATa                                                                  <  2:1148870/151‑1 (MQ=255)                                                                                 aaCTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATa                                                                  <  2:859657/151‑1 (MQ=255)                                                                                  aCTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATaa                                                                 <  2:624504/151‑1 (MQ=255)                                                                                  aCTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATaa                                                                 <  2:259129/151‑1 (MQ=255)                                                                                  aCTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATa                                                                  >  2:561350/1‑150 (MQ=255)                                                                                       aGTTGTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTGAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTCCACCAATCGTTCAACGTCGTGCTGATGAGTGAt                                                            <  2:773831/151‑1 (MQ=255)                                                                                        gTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACGGGAGACGTTTCGTAAATGCTCTAGATTTTTGACTAAAGTTTCGTTAGGATTGGTCTCATCGTATGAATAATTACACCAATCGTACAACGTCTGGCTGATAAGt                                                               <  2:375523/147‑1 (MQ=255)                                                                                           ttCAAATATCTATTTCCGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTATTGCTGATAGGCGATg                                                           >  2:1143559/1‑148 (MQ=255)                                                                                            tCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCg                                                        <  2:1130102/150‑1 (MQ=255)                                                                                            tCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCg                                                        <  2:602082/150‑1 (MQ=255)                                                                                               aaTATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATta                                                    <  1:905991/151‑1 (MQ=255)                                                                                                atatCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATtat                                                   >  1:814928/1‑151 (MQ=255)                                                                                                  atCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTatat                                                 >  2:859521/1‑151 (MQ=255)                                                                                                    cTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATg                                                >  2:67371/1‑150 (MQ=255)                                                                                                      aTTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTatat                                                 <  2:214058/147‑1 (MQ=255)                                                                                                      aTTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAg                                              <  1:825005/150‑1 (MQ=255)                                                                                                        ttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGtt                                           >  1:103736/1‑151 (MQ=255)                                                                                                          cAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTa                                          <  2:1114641/150‑1 (MQ=255)                                                                                                          cAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGCTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGtt                                           >  2:102751/1‑149 (MQ=255)                                                                                                             gTCAAAAGATTGAATTATTCCATGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTTTTTAGGCTGGGACTCATCGAATGATTAATTACACCAATCGTTCATCGTCTTGCTGATAAGTTATGGCGATTATATGAGGGTAg                                         >  2:61349/1‑148 (MQ=255)                                                                                                             gTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCa                                       >  1:628208/1‑150 (MQ=255)                                                                                                              tCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAAt                                     >  2:514878/1‑151 (MQ=255)                                                                                                                   aGATTGAATTATTCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCtt                                <  2:1035407/151‑1 (MQ=255)                                                                                                                          attattCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGaaa                         <  2:1015235/151‑1 (MQ=255)                                                                                                                               tCACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAgg                    >  2:256003/1‑151 (MQ=255)                                                                                                                                cACTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAgg                    >  2:616372/1‑150 (MQ=255)                                                                                                                                 aCTGGAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGaa                  <  1:604023/151‑1 (MQ=255)                                                                                                                                    ggAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAccc               >  2:900760/1‑151 (MQ=255)                                                                                                                                    ggAGACGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAcc                <  1:932016/150‑1 (MQ=255)                                                                                                                                      agaCGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccg             >  1:552166/1‑151 (MQ=255)                                                                                                                                        aCGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccgcc           >  1:978373/1‑151 (MQ=255)                                                                                                                                         cGATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccgccg          >  1:82753/1‑151 (MQ=255)                                                                                                                                          gATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccgccg          >  1:311781/1‑150 (MQ=255)                                                                                                                                          gATTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCcgccgc         >  1:1145561/1‑151 (MQ=255)                                                                                                                                           aTTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCcgcg        >  2:356870/1‑151 (MQ=255)                                                                                                                                           aTTCGTAAATGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCcgcg        >  1:790867/1‑151 (MQ=255)                                                                                                                                                 aaaTGCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTg  >  1:892520/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTG  >  NC_000913/1334902‑1335196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑