Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,459,435 A→C I128L (ATT→CTT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,459,4350AC100.0% 183.8 / NA 48I128L (ATT→CTT) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/24);  total (24/24)

CACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGG  >  NC_000913/1459291‑1459582
                                                                                                                                                 |                                                                                                                                                   
cccgcgtgggccggtGCGGCCCTGGTCATGGAGGCGGCGTCAGACGGTATGAAAGTAAAAAACG‑CCCTCTTTGCACGGCGGGGGGAATTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTtg                                                                                                                                                  <  2:424885/134‑1 (MQ=255)
cACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTtgct                                                                                                                                                >  1:430413/1‑150 (MQ=255)
      gtGGCAGCGGCGGCCTTGTTTTTGGAGGCGGCGTCTAACCTGCTGAAAGAAAAAAAAC‑CGCTTTTCCCACGGCGGGCGTAATTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGgag                                                                                                                                          <  2:687086/149‑1 (MQ=255)
      cTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCgg                                                                                                                                            >  1:894819/1‑148 (MQ=255)
       ttgcagcaGCGGACCTGGTCATTGAAGCGGCGGCTGCATGTCTGGAAGGCAAAAAAG‑CGCTCTTTGCACACCTGGCGGAAGGTCGCCCGCCACAAACGCTATTGACCACTCACACTTCGCCAATCTCTATAACCCAGCCTGCTGCgg                                                                                                                                            >  2:186905/3‑147 (MQ=255)
          cAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAGAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATaa                                                                                                                                      >  1:802995/1‑150 (MQ=255)
                 ggccctggtatgtaaaGGGGTGTCTGAACGTCTGAAAGTAAAAAAGG‑CGCTTTTGCCACGGGTGGCGGATTTTTTCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATc                                                                                                                                <  1:1023553/135‑1 (MQ=255)
                 ggACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCt                                                                                                                              >  2:633779/1‑151 (MQ=255)
                      tGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACg                                                                                                                         >  2:996829/1‑151 (MQ=255)
                           aTTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTg                                                                                                                    >  2:361441/1‑151 (MQ=255)
                                      gTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCAt                                                                                                         >  1:49105/1‑151 (MQ=255)
                                           aacgtcggaaacaaaaaaagG‑CTCTTTTCCCACAGCGGGAGGAATTTGCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCAt                                                                                                         <  2:408711/132‑1 (MQ=255)
                                               tCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAAc                                                                                                >  1:764080/1‑151 (MQ=255)
                                                    aagaaaaaaaaG‑CCCTCTTTGCACGGCTGGGGGAATTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAAcc                                                                                               <  1:779351/142‑1 (MQ=255)
                                                      acaaaaaaaGCCGCTCTTTGCACGGGGGGGGAAAGTTGGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTa                                                                                                  <  2:478547/140‑1 (MQ=255)
                                                       aaaaaaacG‑CTCTCTTTGCACGGCTGGCGGAATTTCGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCAcc                                                                                        <  2:140209/149‑1 (MQ=255)
                                                        aaaaaaaG‑CCCTTTTGCCACGGCGGGGGGAATTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCg                                                                                       <  1:417792/150‑1 (MQ=255)
                                                                    tctTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGtt                                                                            >  1:628851/1‑151 (MQ=255)
                                                                         gCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTtggtgg                                                                       >  2:182122/1‑151 (MQ=255)
                                                                            caGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAgg                                                                    >  1:1128193/1‑151 (MQ=255)
                                                                             aGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggt                                                                   >  1:472255/1‑151 (MQ=255)
                                                                               cTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggtgg                                                                 >  1:340416/1‑151 (MQ=255)
                                                                                 ggggaattttcccccGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggtgg                                                                 <  2:894759/137‑1 (MQ=255)
                                                                                 ggCGGAATTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAggtg                                                                  <  2:509048/148‑1 (MQ=255)
                                                                                          ttGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTg                                                      >  2:48267/1‑151 (MQ=255)
                                                                                          tcgCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCt                                                       <  2:662805/148‑1 (MQ=255)
                                                                                            acccGCCACAAACGCTATTGACCACTAACCCTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTGTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTg                                                      <  2:162251/148‑1 (MQ=255)
                                                                                                 ccACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcgg                                               >  1:1025474/1‑151 (MQ=255)
                                                                                                 ccACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcgg                                               <  1:520083/151‑1 (MQ=255)
                                                                                                      aaCGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAAcggcggcg                                          <  2:10160/151‑1 (MQ=255)
                                                                                                         gCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGaa                                       <  1:61980/151‑1 (MQ=255)
                                                                                                           tATTGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAg                                      >  2:1712/1‑150 (MQ=255)
                                                                                                             ttGACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAgtt                                    <  2:745512/150‑1 (MQ=255)
                                                                                                               gACCACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAgttgtt                                 <  2:430393/151‑1 (MQ=255)
                                                                                                                  cACTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGttgttg                                >  1:62516/1‑149 (MQ=255)
                                                                                                                   aCTAACACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGc                             >  2:1151045/1‑151 (MQ=255)
                                                                                                                      aaCACTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGt                          <  1:694854/151‑1 (MQ=255)
                                                                                                                       acacTTCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAATTGTTGAGCAGtt                         <  2:760950/151‑1 (MQ=255)
                                                                                                                           ttCGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGTAGTTGTTGAGCAGTtgtgt                     >  2:1052574/1‑151 (MQ=255)
                                                                                                                             cGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTtgtgtg                    <  1:1107578/150‑1 (MQ=255)
                                                                                                                             cGTCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGa                   <  2:454001/151‑1 (MQ=255)
                                                                                                                               tCAATCTCTATAACCGCGCTTGCTGCGGAGATAAAAAATACTGAACGTGTTGCGGGACTGCATTTTTTTAACCCGGCACCGGAGATGAAGGTGGTGGAGGGGGTCAGTGGGCTGGCAACGGCGGCGGTAGTTGTTGGGCAGGtgtgtgga                  >  2:594852/1‑148 (MQ=255)
                                                                                                                                    ctctATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGAGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGGGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACg            >  1:306150/1‑151 (MQ=255)
                                                                                                                                      ctATAACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACg            <  1:1206226/149‑1 (MQ=255)
                                                                                                                                        ataACCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGtt          <  2:802953/149‑1 (MQ=255)
                                                                                                                                          aaCCGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGt      >  1:849225/1‑151 (MQ=255)
                                                                                                                                            ccGCGCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGCTg    >  2:456014/1‑151 (MQ=255)
                                                                                                                                             cgcgCTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTgg   <  1:219738/151‑1 (MQ=255)
                                                                                                                                              gcgcTTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTggg  >  2:1200777/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGCTGCATTTTTTTAACCCGGCACCGGTGATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGG  >  NC_000913/1459291‑1459582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑