Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,703,763	A→C	L182L (CTT→CTG)	ydgJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,763	0	A	C	100.0%	211.1 / NA	56	L182L (CTT→CTG)	ydgJ	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/20);  total (36/20)

CCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCAAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTT  >  NC_000913/1703618‑1703908                                                                                                                                                  |                                                                                                                                                  ccAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCcag                                                                                                                                                 <  1:1096975/148‑1 (MQ=255)    gCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGAtg                                                                                                                                           >  2:3243/1‑151 (MQ=255)        ggTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATgtggt                                                                                                                                       <  1:624561/151‑1 (MQ=255)          tACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGc                                                                                                                                     >  2:952267/1‑151 (MQ=255)           aCCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCt                                                                                                                                    >  1:124857/1‑151 (MQ=255)              gtgtAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGCCTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTaaa                                                                                                                                 <  2:482926/151‑1 (MQ=255)                  aaaaTGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGt                                                                                                                             <  1:303512/151‑1 (MQ=255)                     aTGACGCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTAc                                                                                                                           <  2:184026/150‑1 (MQ=255)                       gACGCGCCTCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCCCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAAGGGCCTGATACAGAAGAGGTGGCGCTAAATCGTAc                                                                                                                           >  2:568455/1‑148 (MQ=255)                           cgccgcTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATa                                                                                                                     <  2:926736/150‑1 (MQ=255)                                 tgtgGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGc                                                                                                              >  1:1180482/1‑151 (MQ=255)                                 tgtgGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGc                                                                                                              >  2:53190/1‑151 (MQ=255)                                    ggATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTg                                                                                                           >  1:357619/1‑151 (MQ=255)                                        aGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGgcc                                                                                                       <  1:843345/151‑1 (MQ=255)                                             aaGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCt                                                                                                  >  1:350093/1‑151 (MQ=255)                                               gATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGAGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGt                                                                                                >  1:1134273/1‑151 (MQ=255)                                                  ggCGTGGAAATAATCGGTCGACTGCGCTCCAGGCCGTAACGGTGCCAAATCTACCGTCATGGTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGTTACCGATGCCTGGGCCGTCCTGTTCa                                                                                             <  2:712117/151‑1 (MQ=255)                                                      tGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGcc                                                                                         >  2:367661/1‑151 (MQ=255)                                                       ggAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCa                                                                                        <  1:700927/151‑1 (MQ=255)                                                         aaaTAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAAc                                                                                      >  2:505778/1‑151 (MQ=255)                                                         aaaTAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAAc                                                                                      >  1:1177365/1‑151 (MQ=255)                                                                        tGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGcg                                                                       >  2:574714/1‑151 (MQ=255)                                                                           gcTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGcggac                                                                    <  2:405/151‑1 (MQ=255)                                                                           gcTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGcggac                                                                    <  1:59965/151‑1 (MQ=255)                                                                               cGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGcggacgga                                                                 >  2:1151901/1‑150 (MQ=255)                                                                               cGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGaa                                                                <  2:1207471/151‑1 (MQ=255)                                                                                    cGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGt                                                           <  2:779204/151‑1 (MQ=255)                                                                                     gTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTc                                                          >  1:595782/1‑151 (MQ=255)                                                                                       aaCTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCaa                                                        <  1:240207/151‑1 (MQ=255)                                                                                         cTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCaaa                                                       >  1:1116786/1‑150 (MQ=255)                                                                                           gtgCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCaaaa                                                      <  2:1174566/149‑1 (MQ=255)                                                                                           gtgCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCaaaa                                                      <  1:139272/149‑1 (MQ=255)                                                                                                  aTCTACCGTCATGCTGACCGGTAAACCAAAAAGCGGAATGGCCTGATCCATAAGCTGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCCCGCGAATGAACGCGCCCCTGCGGACGGAAGCGGTCAAAATGATACTc                                              >  2:1158060/1‑150 (MQ=255)                                                                                                   tCTACCGTCATGCTGACCGGTAAACCAAACAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCa                                             >  1:532686/1‑150 (MQ=255)                                                                                                     tACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCaa                                            >  2:964621/1‑149 (MQ=255)                                                                                                     tACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGACTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCaa                                            >  2:150468/1‑149 (MQ=255)                                                                                                      aCCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAg                                          >  2:45943/1‑150 (MQ=255)                                                                                                        cGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTa                                        >  1:13357/1‑150 (MQ=255)                                                                                                         gTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTa                                        >  2:1042386/1‑149 (MQ=255)                                                                                                            aTGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCaa                                   <  2:726132/151‑1 (MQ=255)                                                                                                             tGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGACGCGGTCAAAATGAGACTCAAAGTAAGCAAc                                  >  1:693563/1‑151 (MQ=255)                                                                                                                   ccGGTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGAAACTTCAcc                            >  1:45498/1‑151 (MQ=255)                                                                                                                      gTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCAccc                           >  1:780228/1‑149 (MQ=255)                                                                                                                      gTAAACCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAg                         <  1:626767/151‑1 (MQ=255)                                                                                                                        aaaCCAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGc                        <  1:456348/150‑1 (MQ=255)                                                                                                                           ccAAAAAGCGTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGc                    >  2:256129/1‑151 (MQ=255)                                                                                                                                    gTAATGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAATCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCcgcg            >  1:953357/1‑150 (MQ=255)                                                                                                                                      aaTGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGt         >  2:218203/1‑151 (MQ=255)                                                                                                                                       aTGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGt         >  2:101613/1‑150 (MQ=255)                                                                                                                                       aTGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGt         >  2:348873/1‑150 (MQ=255)                                                                                                                                       aTGGCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCACTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGt         >  1:909830/1‑150 (MQ=255)                                                                                                                                         ggCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTaa       >  2:326617/1‑150 (MQ=255)                                                                                                                                         ggCCTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTaa       >  2:883684/1‑150 (MQ=255)                                                                                                                                         ggCCTGATCCAGAAGATGTGGTGCTAAATCGCACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTa        <  2:594471/149‑1 (MQ=255)                                                                                                                                           ccTGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAAcc    >  1:252856/1‑151 (MQ=255)                                                                                                                                             tGATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCtt  >  2:1151176/1‑151 (MQ=255)                                                                                                                                              gATCCAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCtt  <  1:20111/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCGTGGAAATAATCGGTCGACTGCGCTCCGGGCCGTAACTGTGCCAAATCTACCGTCATGCTGACCGGTAAACCAAAAAGCGTAATGGCCTGATCAAGAAGATGTGGTGCTAAATCGTACCAGATACCGCTGCCTGGGCCGCCCTGTTCACGCCAACGATCGCGCACCTGCGGACGGAAGCGGTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTT  >  NC_000913/1703618‑1703908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑