Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,924,700 T→G intergenic (‑587/+35) yqcC ← / ← syd DUF446 family protein/SecY‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,924,7000TG99.0% 377.5 / ‑4.4 99intergenic (‑587/+35)yqcC/sydDUF446 family protein/SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (64/34);  total (65/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTACACAC  >  NC_000913/2924556‑2924845
                                                                                                                                                |                                                                                                                                                 
aCAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAag                                                                                                                                                >  1:690416/1‑148 (MQ=255)
      cgcTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGAtctctc                                                                                                                                       <  1:1071422/151‑1 (MQ=255)
          tACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAGTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTcac                                                                                                                                     <  2:1007639/149‑1 (MQ=255)
               aCAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAAcc                                                                                                                                 >  2:512375/1‑148 (MQ=255)
               aCAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGa                                                                                                                              <  1:121678/151‑1 (MQ=255)
                cAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACGAGAt                                                                                                                             <  2:8926/151‑1 (MQ=255)
                 aaTGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATg                                                                                                                            <  1:1070298/151‑1 (MQ=255)
                  aTGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATgg                                                                                                                           <  2:847928/151‑1 (MQ=255)
                    gCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATgggg                                                                                                                         >  2:989586/1‑151 (MQ=255)
                     ccTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        <  1:890963/151‑1 (MQ=255)
                      cTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        >  1:816139/1‑150 (MQ=255)
                      cTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGt                                                                                                                        >  2:257920/1‑150 (MQ=255)
                       ttAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAt                                                                                                                      >  2:728201/1‑151 (MQ=255)
                       ttAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAt                                                                                                                      >  1:683897/1‑151 (MQ=255)
                         aaGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTAttt                                                                                                                    >  2:415900/1‑151 (MQ=255)
                               aaTTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGAtt                                                                                                               >  1:255830/1‑150 (MQ=255)
                                 tttCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTCATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                                                                                                             >  2:1048942/1‑150 (MQ=255)
                                  ttCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                                                                                                             >  1:607148/1‑149 (MQ=255)
                                  ttCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACa                                                                                                            <  1:770078/150‑1 (MQ=255)
                                   tCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                                                                                                             >  2:155133/1‑148 (MQ=255)
                                   tCATATGAAAGTTAAGAATTAACAATCAACATCTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                                                                                                             >  1:202718/1‑148 (MQ=255)
                                    cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                                                                                                          <  1:898220/150‑1 (MQ=255)
                                    cATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                                                                                                          >  1:984767/1‑150 (MQ=255)
                                        tGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAgg                                                                                                     >  1:1034767/1‑151 (MQ=255)
                                        tGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAgg                                                                                                     >  1:931815/1‑151 (MQ=255)
                                        tGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAgg                                                                                                     >  1:341301/1‑151 (MQ=255)
                                             gTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTaa                                                                                                <  2:455329/151‑1 (MQ=255)
                                               tAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGt                                                                                              >  2:541039/1‑151 (MQ=255)
                                                aaGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGtt                                                                                             <  2:569291/151‑1 (MQ=255)
                                                aaGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGtt                                                                                             >  1:956846/1‑151 (MQ=255)
                                                  gAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGa                                                                                           <  1:800692/151‑1 (MQ=255)
                                                   aaTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGAt                                                                                          >  1:534696/1‑151 (MQ=255)
                                                    aTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGAtt                                                                                         >  1:48215/1‑151 (MQ=255)
                                                      tAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTa                                                                                        <  1:481710/150‑1 (MQ=255)
                                                      tAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTa                                                                                        >  1:609175/1‑150 (MQ=255)
                                                      tAACAATCAACATTTTGATTTTAAATGAAAATAACGAATCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTa                                                                                        >  2:672458/1‑150 (MQ=255)
                                                       aaCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTaa                                                                                       <  1:482900/150‑1 (MQ=255)
                                                       aaCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAg                                                                                      <  1:1054070/151‑1 (MQ=255)
                                                        aCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAgg                                                                                     >  2:576276/1‑151 (MQ=255)
                                                         cAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAgg                                                                                     >  1:83520/1‑150 (MQ=255)
                                                          aaTCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGaa                                                                                   <  1:504917/151‑1 (MQ=255)
                                                           aTCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAAt                                                                                  >  1:299282/1‑151 (MQ=255)
                                                               aCATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCg                                                                              >  1:1021170/1‑151 (MQ=255)
                                                                 aTTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGc                                                                             >  1:1169605/1‑150 (MQ=255)
                                                                 aTTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGc                                                                             >  1:589469/1‑150 (MQ=255)
                                                                   tttGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCaa                                                                           <  2:896553/150‑1 (MQ=255)
                                                                     tGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCa                                                                            >  1:940083/1‑147 (MQ=255)
                                                                     tGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGAATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCa                                                                            >  2:1185075/1‑147 (MQ=255)
                                                                      gATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAtt                                                                       <  2:537626/151‑1 (MQ=255)
                                                                      gATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGAtt                                                                       >  2:699290/1‑151 (MQ=255)
                                                                           tAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAgg                                                                  >  1:355054/1‑151 (MQ=255)
                                                                            aaaTGAAAATACCGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACCACCAGATGGGGTATTTGGATTATAGAAGAGGCTTAAGTTGACTAAGGCATTACGCAAGATTTg                                                                     >  2:660377/1‑147 (MQ=255)
                                                                            aaaTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAgg                                                                  <  1:1076955/150‑1 (MQ=255)
                                                                            aaaTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGc                                                                 >  2:912383/1‑151 (MQ=255)
                                                                             aaTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCt                                                                >  1:1155329/1‑151 (MQ=255)
                                                                              aTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTa                                                               <  1:699181/151‑1 (MQ=255)
                                                                                 aaaaTAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAAt                                                            <  1:524213/151‑1 (MQ=255)
                                                                                  aaaTAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAAt                                                            >  1:917636/1‑150 (MQ=255)
                                                                                  aaaTAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATg                                                           >  1:533373/1‑151 (MQ=255)
                                                                                    aTAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCg                                                         <  1:379862/151‑1 (MQ=255)
                                                                                      atcGATTCTTATCTTTTTAAGAATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCATATGGGGAATTTGGATAACAGAAGAGGCTTAAGTTGATGAAGGAATTCCGCAAGATTTGAGGCTAAATGAGt                                                        >  2:854716/3‑150 (MQ=255)
                                                                                      aaCGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTc                                                       >  1:386175/1‑151 (MQ=255)
                                                                                         gATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGt                                                    <  1:541064/151‑1 (MQ=255)
                                                                                         gATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGt                                                    <  1:730823/151‑1 (MQ=255)
                                                                                          aTTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGtt                                                   >  1:898316/1‑151 (MQ=255)
                                                                                           ttCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGttt                                                  >  2:1189489/1‑151 (MQ=255)
                                                                                            tCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGttt                                                  >  2:687150/1‑150 (MQ=255)
                                                                                            tCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGttt                                                  >  2:526820/1‑150 (MQ=255)
                                                                                               tATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTAc                                                <  2:568462/149‑1 (MQ=255)
                                                                                               tATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACgt                                              >  2:607862/1‑151 (MQ=255)
                                                                                                aTCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACgtg                                             >  1:301890/1‑151 (MQ=255)
                                                                                                 tCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCTGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACgtgt                                            >  2:352922/1‑151 (MQ=255)
                                                                                                    ttttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTcc                                         >  2:976535/1‑151 (MQ=255)
                                                                                                    ttttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTc                                          <  2:83965/150‑1 (MQ=255)
                                                                                                      ttAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTccc                                        <  2:297358/150‑1 (MQ=255)
                                                                                                        aaGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAg                                      <  1:24063/150‑1 (MQ=255)
                                                                                                        aaGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGc                                     <  2:876736/151‑1 (MQ=255)
                                                                                                         aGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCg                                    <  1:368887/151‑1 (MQ=255)
                                                                                                         aGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCg                                    >  1:153373/1‑151 (MQ=255)
                                                                                                             ttCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGtctc                                >  2:215985/1‑151 (MQ=255)
                                                                                                             ttCGCTTATTTCCTGGCAATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGtctc                                >  2:459384/1‑151 (MQ=255)
                                                                                                              tCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAACTGCGTCCGTTTACGTGTTCCCAGCGTctct                               >  1:672115/1‑151 (MQ=255)
                                                                                                              tCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTctct                               >  1:20356/1‑151 (MQ=255)
                                                                                                              tCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTctct                               >  1:987356/1‑151 (MQ=255)
                                                                                                               cGCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCtt                              >  1:696012/1‑151 (MQ=255)
                                                                                                                gCTTATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCttt                             <  1:409366/151‑1 (MQ=255)
                                                                                                                  ttATTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTg                            >  1:1031486/1‑150 (MQ=255)
                                                                                                                    aTTTCCTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGcac                         >  1:394672/1‑151 (MQ=255)
                                                                                                                        ccTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACtt                     >  1:1185985/1‑151 (MQ=255)
                                                                                                                        ccTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACt                      <  1:921370/150‑1 (MQ=255)
                                                                                                                         cTGGCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTc                    >  2:803305/1‑151 (MQ=255)
                                                                                                                           ggCGATCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCa                   <  1:1035877/150‑1 (MQ=255)
                                                                                                                            gCGATCTCCTACAGAGTCTGGAAGAGATCTATCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCAcc                 <  1:672491/151‑1 (MQ=255)
                                                                                                                               aTCTCCTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCgg               >  2:349460/1‑150 (MQ=255)
                                                                                                                                    cTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGgag             >  2:640487/1‑147 (MQ=255)
                                                                                                                                    cTACAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGgag             >  1:939815/1‑147 (MQ=255)
                                                                                                                                      aCAGAGTCTGGAAGAGATCTCTCACAACCAGATGGGGCATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGAtt        >  2:134974/1‑150 (MQ=255)
                                                                                                                                          agTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTacac    >  1:686289/1‑150 (MQ=255)
                                                                                                                                           gTCTGGAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTacacac  >  2:127244/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAGGAATTCCGCAAGATTTGAGGCTAAATGCGTCCGTTTACGTGTTCCCAGCGTCTCTTTGCACACTTCACCGGAGAGATTACACAC  >  NC_000913/2924556‑2924845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑