Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,902	T→G	D85A (GAT→GCT)	thyA ←	thymidylate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,902	0	T	G	100.0%	343.9 / NA	88	D85A (GAT→GCT)	thyA	thymidylate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/48);  total (40/48)

TTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTA  >  NC_000913/2964755‑2965048                                                                                                                                                    |                                                                                                                                                   ttCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCgg                                                                                                                                                 >  2:468539/1‑151 (MQ=255) ttCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCgg                                                                                                                                                 >  1:320803/1‑151 (MQ=255)    cACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGccc                                                                                                                                              <  1:933599/151‑1 (MQ=255)     aCGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCa                                                                                                                                             <  2:503212/151‑1 (MQ=255)          gAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGt                                                                                                                                        <  2:134951/151‑1 (MQ=255)           aaaCAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTc                                                                                                                                       >  2:216332/1‑151 (MQ=255)             aCAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTccc                                                                                                                                     <  2:539470/151‑1 (MQ=255)              cAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTccc                                                                                                                                     >  2:116435/1‑150 (MQ=255)              cAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCa                                                                                                                                    <  2:619105/151‑1 (MQ=255)                ataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTccc                                                                                                                                     >  1:994260/1‑148 (MQ=255)                  aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATg                                                                                                                                <  1:89085/151‑1 (MQ=255)                  aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATg                                                                                                                                <  1:929283/151‑1 (MQ=255)                     gcggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTg                                                                                                                             <  1:684452/151‑1 (MQ=255)                      cggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTg                                                                                                                             >  2:1174038/1‑150 (MQ=255)                      cggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTg                                                                                                                             <  2:171482/150‑1 (MQ=255)                      cggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGa                                                                                                                            <  1:126536/151‑1 (MQ=255)                       ggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGAc                                                                                                                           <  1:535773/151‑1 (MQ=255)                        gcgcAAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACa                                                                                                                          <  2:35537/151‑1 (MQ=255)                         cgcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACAt                                                                                                                         >  1:250757/1‑151 (MQ=255)                            gAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGt                                                                                                                      <  1:343402/151‑1 (MQ=255)                            gAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGt                                                                                                                      <  1:779470/151‑1 (MQ=255)                              aTCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGttt                                                                                                                    <  2:821078/151‑1 (MQ=255)                                ccGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTc                                                                                                                  <  2:902346/151‑1 (MQ=255)                                 cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                                                                                                                 >  1:815261/1‑151 (MQ=255)                                 cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                                                                                                                 >  2:59628/1‑151 (MQ=255)                                 cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                                                                                                                 >  1:303588/1‑151 (MQ=255)                                      cGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTa                                                                                                             <  2:355008/150‑1 (MQ=255)                                      cGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTa                                                                                                             <  1:737028/150‑1 (MQ=255)                                       gTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGa                                                                                                           <  1:659454/151‑1 (MQ=255)                                       gTTTTTCAGCTGGTTCAGTACCGTAGGGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGTCATTGTTTTCgtgt                                                                                                              >  2:844250/1‑148 (MQ=255)                                         ttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGAt                                                                                                          >  2:1120394/1‑150 (MQ=255)                                          tttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         >  2:87072/1‑150 (MQ=255)                                          tttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         <  2:1132860/150‑1 (MQ=255)                                          tttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         <  1:1084570/150‑1 (MQ=255)                                           ttCAGCTGGGTCAGGACCGTAGTGATCTGGTCAATATGAAGACCATCTGGCGTTGGCTAGGCGCGCCACTGTTTACCATACACTGGACCGAGGTCGCCGTTTTCAGCCGCCCATTCCTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                                                         >  2:172097/1‑149 (MQ=255)                                              aGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCa                                                                                                     >  1:536775/1‑150 (MQ=255)                                               gCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAAt                                                                                                   >  2:976681/1‑151 (MQ=255)                                                cTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATg                                                                                                  >  2:1155272/1‑151 (MQ=255)                                                  ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATg                                                                                                  <  2:803694/149‑1 (MQ=255)                                                  ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATg                                                                                                  <  1:1049487/149‑1 (MQ=255)                                                  ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt                                                                                                >  1:572592/1‑151 (MQ=255)                                                     tCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgt                                                                                             >  1:497593/1‑151 (MQ=255)                                                      cAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgtg                                                                                            <  1:173090/151‑1 (MQ=255)                                                               aGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTg                                                                                    <  1:147921/150‑1 (MQ=255)                                                               aGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGc                                                                                   <  2:640343/151‑1 (MQ=255)                                                                gTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCa                                                                                  <  1:962281/151‑1 (MQ=255)                                                                       ggTCAATATGACGACCATCTGGCGTTGGCCAGGGGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATCCGTCGCAGTTGGAGACATTGTTTTCGTGTAGGTAAGCAATGTTAGGGTCGCCATCCAGAAAcc                                                                            >  2:1062605/1‑150 (MQ=255)                                                                       ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAAcc                                                                            >  1:1111670/1‑150 (MQ=255)                                                                       ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAAc                                                                             <  1:301877/149‑1 (MQ=255)                                                                        gTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACca                                                                           <  2:622037/150‑1 (MQ=255)                                                                          cAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCAcag                                                                        <  2:749703/151‑1 (MQ=255)                                                                                  cgacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCAt                                                                >  1:725286/1‑151 (MQ=255)                                                                                   gacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATg                                                               >  2:914804/1‑151 (MQ=255)                                                                                    acCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATgg                                                              >  2:900356/1‑151 (MQ=255)                                                                                      cATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgat                                                            <  1:19899/151‑1 (MQ=255)                                                                                       aTCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgatg                                                           >  2:473127/1‑151 (MQ=255)                                                                                         cTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGgatgat                                                         >  2:777272/1‑151 (MQ=255)                                                                                             cGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGa                                                      >  2:365141/1‑150 (MQ=255)                                                                                              gTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGaa                                                     >  1:965693/1‑150 (MQ=255)                                                                                                tGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAAc                                                    <  2:798012/149‑1 (MQ=255)                                                                                                  gCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGc                                                  >  1:656035/1‑149 (MQ=255)                                                                                                    cAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGt                                              <  1:865044/151‑1 (MQ=255)                                                                                                     aGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTg                                             <  1:719294/151‑1 (MQ=255)                                                                                                     aGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTg                                             >  1:793809/1‑151 (MQ=255)                                                                                                          cgcCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAAc                                        <  1:842032/151‑1 (MQ=255)                                                                                                          cgcCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAAc                                        <  1:290923/151‑1 (MQ=255)                                                                                                            ccACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGt                                      <  1:154046/151‑1 (MQ=255)                                                                                                             cACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGtt                                     <  2:375904/151‑1 (MQ=255)                                                                                                             cACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGtt                                     >  2:242006/1‑151 (MQ=255)                                                                                                                 gTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAg                                  <  2:715103/150‑1 (MQ=255)                                                                                                                 gTTTACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGt                                 >  2:1081080/1‑151 (MQ=255)                                                                                                                  tttACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGtt                                >  1:376487/1‑151 (MQ=255)                                                                                                                    tACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTg                               <  2:931227/150‑1 (MQ=255)                                                                                                                    tACCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGt                              >  1:427357/1‑151 (MQ=255)                                                                                                                     aCCATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGt                              <  1:87077/150‑1 (MQ=255)                                                                                                                      ccATACACTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTc                             >  1:930366/1‑150 (MQ=255)                                                                                                                          acacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCa                         <  2:552501/150‑1 (MQ=255)                                                                                                                          acacTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCa                         <  1:137921/150‑1 (MQ=255)                                                                                                                            acTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCg                      <  1:426041/151‑1 (MQ=255)                                                                                                                             cTGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCgg                     >  1:289805/1‑151 (MQ=255)                                                                                                                              tGGCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCggg                    >  1:1015241/1‑151 (MQ=255)                                                                                                                               ggCCCGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCgg                     <  2:542516/149‑1 (MQ=255)                                                                                                                                 cccGAGGTCGCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAAt                 <  2:308516/151‑1 (MQ=255)                                                                                                                                          gCCGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGc        <  1:874389/151‑1 (MQ=255)                                                                                                                                            cGTTTTCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGc        >  2:85822/1‑149 (MQ=255)                                                                                                                                                ttCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGtt   >  1:782157/1‑150 (MQ=255)                                                                                                                                                ttCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGtt   >  1:78301/1‑150 (MQ=255)                                                                                                                                                 tCAGCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTa  <  2:573059/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTA  >  NC_000913/2964755‑2965048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑