Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,568	A→C	D213E (GAT→GAG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,568	0	A	C	100.0%	236.5 / NA	66	D213E (GAT→GAG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/35);  total (31/35)

GGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCC  >  NC_000913/3035421‑3035714                                                                                                                                                    |                                                                                                                                                   ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTcc                                                                                                                                                 <  2:492693/151‑1 (MQ=255) ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTc                                                                                                                                                  <  1:235328/150‑1 (MQ=255)       aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATTCGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCggg                                                                                                                                           >  1:453134/1‑151 (MQ=255)       aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCggg                                                                                                                                           >  2:204321/1‑151 (MQ=255)          tttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTg                                                                                                                                        >  1:639263/1‑151 (MQ=255)            tGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAt                                                                                                                                      >  2:566957/1‑151 (MQ=255)             gTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGAtt                                                                                                                                     <  2:582796/151‑1 (MQ=255)                  tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTc                                                                                                                                   <  2:487037/148‑1 (MQ=255)                   gtgCTGGGAACGGTCGTTCTGGCACTGAGTCACGATCCCGGTCGGGATGTGGGTAATACGCTCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCACCGGACGTGCGATAATCGTCAATGCGCAGCTCCGCCGGGTTGATTTCGata                                                                                                                               <  2:1047151/151‑1 (MQ=255)                     gCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatc                                                                                                                             >  2:110053/1‑151 (MQ=255)                     gCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatat                                                                                                                              <  2:195926/150‑1 (MQ=255)                        gggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatca                                                                                                                            <  2:956145/149‑1 (MQ=255)                         ggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaata                                                                                                                         <  1:69975/151‑1 (MQ=255)                          gAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatat                                                                                                                        <  1:462271/151‑1 (MQ=255)                           aaCGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatatc                                                                                                                       >  1:489566/1‑151 (MQ=255)                           aaCGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatatc                                                                                                                       >  1:494618/1‑151 (MQ=255)                            aCGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatatca                                                                                                                      <  2:594661/151‑1 (MQ=255)                            aCGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGatatcaatatc                                                                                                                       >  2:879964/1‑150 (MQ=255)                             cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcat                                                                                                                     <  1:803447/151‑1 (MQ=255)                              ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatc                                                                                                                    <  2:575053/151‑1 (MQ=255)                              ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATatcatc                                                                                                                    <  1:29391/151‑1 (MQ=255)                                    tCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCaa                                                                                                               <  1:570139/150‑1 (MQ=255)                                       ggCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTc                                                                                                           <  2:356981/151‑1 (MQ=255)                                        gCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTcc                                                                                                          <  2:20066/151‑1 (MQ=255)                                         cACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCg                                                                                                         <  2:231194/151‑1 (MQ=255)                                             gggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAt                                                                                                      >  2:635641/1‑150 (MQ=255)                                             gggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATa                                                                                                     <  1:98031/151‑1 (MQ=255)                                                tCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaac                                                                                                  >  1:1058574/1‑151 (MQ=255)                                                 cACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaac                                                                                                  >  2:1018320/1‑150 (MQ=255)                                                    ggtCCCGGTCGGGTTGTCGGCAATAATCACCGCAGATTCGGGGCGGTTAACGAGCTGACCGCCCGCGCCGGACGTGCGCTAAACGTCAATGCCTAGCCCTGCCAGGCTGATTTCGATATGAAAATCATCCTCAACTTCCGGATaaacaa                                                                                                >  2:483192/3‑149 (MQ=255)                                                    gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac                                                                                              >  1:172595/1‑151 (MQ=255)                                                     aTCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcg                                                                                             >  1:207589/1‑151 (MQ=255)                                                        ccGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCgg                                                                                          >  1:747181/1‑151 (MQ=255)                                                          ggTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCgg                                                                                          >  2:436815/1‑149 (MQ=255)                                                                gATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAac                                                                                  >  1:353478/1‑151 (MQ=255)                                                                         aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGtgtg                                                                          <  1:1024312/150‑1 (MQ=255)                                                                         aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTgg                                                                         <  1:55087/151‑1 (MQ=255)                                                                             cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgac                                                                     <  1:1093709/151‑1 (MQ=255)                                                                             cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgac                                                                     <  1:42347/151‑1 (MQ=255)                                                                              gCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgacg                                                                    >  1:550894/1‑151 (MQ=255)                                                                                     aGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGAccgccg                                                             <  2:541176/151‑1 (MQ=255)                                                                                       aTTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCgg                                                            >  2:177470/1‑150 (MQ=255)                                                                                       aTTCGGAACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCgg                                                            >  2:334809/1‑150 (MQ=255)                                                                                         tCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAg                                                          <  2:424323/150‑1 (MQ=255)                                                                                          cGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTc                                                        <  2:975110/151‑1 (MQ=255)                                                                                            gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAT‑AACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCaa                                                      >  2:701731/1‑150 (MQ=255)                                                                                               cGGTTAACGTGCTGACCGCCCGTGCCGGACGCGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACg                                                   <  1:609376/151‑1 (MQ=255)                                                                                               cGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACg                                                   >  1:572420/1‑151 (MQ=255)                                                                                               cGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACg                                                   >  1:788801/1‑151 (MQ=255)                                                                                                   tAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGc                                                <  2:821755/150‑1 (MQ=255)                                                                                                        tGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTa                                           <  1:350031/150‑1 (MQ=255)                                                                                                           tGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGc                                        >  1:541086/1‑150 (MQ=255)                                                                                                                gcccgcGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTAGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAgg                                  >  2:777006/1‑151 (MQ=255)                                                                                                                 cccgcGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGATTTTACGCACCAGGc                                 >  1:207336/1‑151 (MQ=255)                                                                                                                  ccgcGCCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCg                                <  2:1030429/151‑1 (MQ=255)                                                                                                                    gcgcCGGACGTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGTATAAGCAAACGCGGAGCTGAACGACGTGTGGCGCCGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGt                              <  1:72473/151‑1 (MQ=255)                                                                                                                             gTGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCg                      <  2:482158/150‑1 (MQ=255)                                                                                                                               gCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGt                    <  1:391045/150‑1 (MQ=255)                                                                                                                               gCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGt                    <  1:864555/150‑1 (MQ=255)                                                                                                                                cGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGttt                  >  2:785341/1‑151 (MQ=255)                                                                                                                                 gATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTc                 >  2:684421/1‑151 (MQ=255)                                                                                                                                  aTAAACGTCAATGCGCAGCTCCGCCGAGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTAAAACGGGTTTTTACGCACCAGGCGGCGAACGACgg                     >  2:1032926/1‑146 (MQ=255)                                                                                                                                        gTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGc          >  1:513204/1‑151 (MQ=255)                                                                                                                                          cAATGCGCAGCTCCCCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAAGCGGTGAACGCCGGTTTCTGTACGc          >  1:826060/1‑149 (MQ=255)                                                                                                                                            aTGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTGCGcagca      <  2:17425/151‑2 (MQ=255)                                                                                                                                                gcAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCagccagcc  <  1:77033/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCC  >  NC_000913/3035421‑3035714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑