Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,656	T→G	E419A (GAA→GCA)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,656	0	T	G	100.0%	334.6 / NA	86	E419A (GAA→GCA)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (45/41);  total (45/41)

ACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGC  >  NC_000913/3084508‑3084799                                                                                                                                                     |                                                                                                                                                aCGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCa                                                                                                                                               <  2:1076254/151‑1 (MQ=255)      tttGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGc                                                                                                                                            >  1:548000/1‑149 (MQ=255)         gCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCagag                                                                                                                                        <  2:340828/150‑1 (MQ=255)         gCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAgaga                                                                                                                                       <  1:641746/151‑1 (MQ=255)              tCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGc                                                                                                                                  >  1:95345/1‑151 (MQ=255)               ccAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGc                                                                                                                                  <  1:876806/150‑1 (MQ=255)               ccAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCg                                                                                                                                 <  2:1195899/151‑1 (MQ=255)                cAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCg                                                                                                                                 >  1:739221/1‑150 (MQ=255)                cAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCg                                                                                                                                 >  1:162179/1‑150 (MQ=255)                     gctTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGTAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGTTCCAGCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTcc                                                                                                                           <  2:971061/151‑1 (MQ=255)                     gctTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTcc                                                                                                                           <  2:703308/151‑1 (MQ=255)                          tGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGtt                                                                                                                      >  2:338478/1‑151 (MQ=255)                           gCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGtt                                                                                                                      <  2:376471/150‑1 (MQ=255)                           gCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTc                                                                                                                     <  2:863677/151‑1 (MQ=255)                             aCTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGt                                                                                                                   <  2:276911/151‑1 (MQ=255)                             aCTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGt                                                                                                                   >  1:487247/1‑151 (MQ=255)                                tCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTg                                                                                                                  <  1:980106/149‑1 (MQ=255)                                  aTGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCAt                                                                                                               >  1:987159/1‑150 (MQ=255)                                    ggCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCAtct                                                                                                             <  1:1192875/150‑1 (MQ=255)                                      cacaTGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCt                                                                                                          <  1:434440/151‑1 (MQ=255)                                       acaTGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTg                                                                                                         >  2:168847/1‑151 (MQ=255)                                         ttGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGcc                                                                                                       >  1:1150161/2‑151 (MQ=255)                                         aTGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGcc                                                                                                       >  2:157949/1‑151 (MQ=255)                                          tGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGcc                                                                                                       >  1:990502/1‑150 (MQ=255)                                              cAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGtt                                                                                                  >  1:1057395/1‑151 (MQ=255)                                                  tAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTccc                                                                                              >  2:1143438/1‑151 (MQ=255)                                                     aGCTGCTCATCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCcac                                                                                            >  1:965389/1‑150 (MQ=255)                                                      gctgctCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACAt                                                                                          <  1:909060/151‑1 (MQ=255)                                                            cAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCttt                                                                                     <  2:270242/150‑1 (MQ=255)                                                            cAGCCCATGCACGCTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCttt                                                                                     <  2:1195959/150‑1 (MQ=255)                                                                ccATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAgc                                                                                <  2:997016/151‑1 (MQ=255)                                                                  aTGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAgcgc                                                                              <  2:464701/151‑1 (MQ=255)                                                                     cACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAgcacgcg                                                                           <  2:643220/151‑1 (MQ=255)                                                                     cACGTTCTTGCAGGCTAAAGATGACGGAAGAGAAGCCGATATGAATGGCGAACAGATACATCAGACTGGTGTGCAACCTTGAACGCAGAGAACGGCGAGTTGCCGGTTCGGGCATCTCCTGCCAGATTTCCCACATGCTTTGCAgcgc                                                                              >  2:501759/1‑148 (MQ=255)                                                                             tGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCgcggcg                                                                      <  2:458394/148‑1 (MQ=255)                                                                               cAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAtct                                                                 >  2:459195/1‑151 (MQ=255)                                                                                aGGCTAAAGATGCCGGAAGAGTGGCGGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCGCGCATAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAt                                                                   <  2:1043405/148‑1 (MQ=255)                                                                                aGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAtctt                                                                >  1:1079708/1‑151 (MQ=255)                                                                                aGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAtctt                                                                <  2:343388/151‑1 (MQ=255)                                                                                  gCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAtcttct                                                              >  1:1027940/1‑151 (MQ=255)                                                                                  gCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCAtcttct                                                              <  2:30737/151‑1 (MQ=255)                                                                                     aaaGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGc                                                            >  2:224584/1‑150 (MQ=255)                                                                                       aGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAgg                                                         <  2:320613/151‑1 (MQ=255)                                                                                         aTGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAgg                                                         >  2:67468/1‑149 (MQ=255)                                                                                         aTGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGgcg                                                       >  2:653276/1‑151 (MQ=255)                                                                                         aTGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGgcg                                                       <  2:322233/151‑1 (MQ=255)                                                                                         aTGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGgcg                                                       >  2:817820/1‑151 (MQ=255)                                                                                         aTGCCGGAAGAGTAGCCGATATGAATATCGTGCATATCTATTTGACTGGCGTGTAACCATGCACGCAGATAACGCAAAATTCCCGGTTAGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGgc                                                        >  2:44530/1‑150 (MQ=255)                                                                                            ccGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCgg                                                    <  1:105512/151‑1 (MQ=255)                                                                                              ggAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTc                                                  >  1:892586/1‑151 (MQ=255)                                                                                              ggAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTc                                                  >  2:724573/1‑151 (MQ=255)                                                                                              ggAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTc                                                  >  2:402189/1‑151 (MQ=255)                                                                                              ggAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTc                                                  <  2:224605/151‑1 (MQ=255)                                                                                               gAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCg                                                 <  1:879515/151‑1 (MQ=255)                                                                                                  gagTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCa                                              <  2:95034/151‑1 (MQ=255)                                                                                                    gTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCAcc                                            <  2:1115967/151‑1 (MQ=255)                                                                                                      aGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCg                                           >  1:778293/1‑150 (MQ=255)                                                                                                        ccGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCgtgt                                        <  2:857638/151‑1 (MQ=255)                                                                                                        ccGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCgtgt                                        <  2:378628/151‑1 (MQ=255)                                                                                                         cGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCgtgt                                        >  2:1176140/1‑150 (MQ=255)                                                                                                         cGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCgtgt                                        >  1:825414/1‑150 (MQ=255)                                                                                                         cGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCgtgt                                        >  2:591176/1‑150 (MQ=255)                                                                                                           atatGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAtt                                     >  1:354864/1‑151 (MQ=255)                                                                                                            tatGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAtt                                     <  1:158766/150‑1 (MQ=255)                                                                                                             atGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCg                                   >  1:837253/1‑151 (MQ=255)                                                                                                              tGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGt                                  >  1:1029515/1‑151 (MQ=255)                                                                                                                aaTGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGtt                                 >  2:915031/1‑150 (MQ=255)                                                                                                                 aTGTCGTGCAGATCCATCTGACTGTCGTGTAGCCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTAc                               <  1:1070883/151‑1 (MQ=255)                                                                                                                  tGTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACg                              >  1:1210621/1‑151 (MQ=255)                                                                                                                   gTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGt                             <  1:538944/151‑1 (MQ=255)                                                                                                                   gTCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGt                             <  1:233963/151‑1 (MQ=255)                                                                                                                    tCGTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGtt                            >  1:415954/1‑151 (MQ=255)                                                                                                                      gTGCAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTcc                          <  1:60747/151‑1 (MQ=255)                                                                                                                         cAGATCCATCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACg                       >  2:681454/1‑151 (MQ=255)                                                                                                                                aTCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCg                    <  1:283038/147‑1 (MQ=255)                                                                                                                                aTCTGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCCCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGCGTATTCGTTACGTTCCACGCCg                    >  1:1035886/1‑147 (MQ=255)                                                                                                                                   tGACTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGatat             >  2:9004/1‑151 (MQ=255)                                                                                                                                     aCTGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGATTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATAtt            >  1:412532/1‑150 (MQ=255)                                                                                                                                       tGTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATAtt            >  2:155004/1‑148 (MQ=255)                                                                                                                                        gTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAg          <  2:987093/149‑1 (MQ=255)                                                                                                                                        gTCGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGa         <  2:1021304/150‑1 (MQ=255)                                                                                                                                          cGTGTAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGacac      >  2:1062857/1‑151 (MQ=255)                                                                                                                                           gtgtAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACAcc     >  1:417515/1‑151 (MQ=255)                                                                                                                                            tgtAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACAcc     >  2:930829/1‑150 (MQ=255)                                                                                                                                              tAACCATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGc  >  1:702432/1‑151 (MQ=255)                                                                                                                                                 ccATGCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGc  <  2:1035574/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                ACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGC  >  NC_000913/3084508‑3084799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑