Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,182,139 T→G Q90P (CAG→CCG)  ygiD ← 4,5‑ DOPA‑extradiol‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,182,1390TG98.7% 259.6 / ‑3.2 74Q90P (CAG→CCG) ygiD4,5‑ DOPA‑extradiol‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (47/26);  total (48/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

AGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCG  >  NC_000913/3181991‑3182286
                                                                                                                                                    |                                                                                                                                                   
aGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCggt                                                                                                                                                   <  1:343736/151‑1 (MQ=255)
 ggTTTGCTACTGTCGATACTCAACTGCCCCATCGGGATATCAGCGCCAGGATACATCTTAATCATCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCggt                                                                                                                                                   <  2:1174639/150‑1 (MQ=255)
             tCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGc                                                                                                                                       >  2:770692/1‑150 (MQ=255)
              cGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCg                                                                                                                                      <  1:1190611/150‑1 (MQ=255)
              cGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCg                                                                                                                                      >  2:878669/1‑150 (MQ=255)
                   cTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCg                                                                                                                                >  1:1203046/1‑151 (MQ=255)
                     cAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCgg                                                                                                                               >  1:815280/1‑150 (MQ=255)
                      aaCTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCgg                                                                                                                               >  2:1197204/1‑149 (MQ=255)
                      aaCTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCACACGCGGTGCCAGCGCAGGCGAACCCgg                                                                                                                               >  2:70921/1‑149 (MQ=255)
                       aCTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGc                                                                                                                            >  1:343489/1‑151 (MQ=255)
                       aCTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGc                                                                                                                            >  2:498567/1‑151 (MQ=255)
                         tGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGc                                                                                                                            >  2:650697/1‑149 (MQ=255)
                          gCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAgg                                                                                                                         <  2:164360/151‑1 (MQ=255)
                          gCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAgg                                                                                                                         <  2:830745/151‑1 (MQ=255)
                           cACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCa                                                                                                                           <  2:1021523/148‑1 (MQ=255)
                           cACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGa                                                                                                                        <  2:452540/151‑1 (MQ=255)
                              cATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATa                                                                                                                      >  1:566821/1‑150 (MQ=255)
                                  gggATATCCGCGTCAGGATACATCTTAATCAGCCCGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCACCCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAAt                                                                                                                    <  2:773625/148‑1 (MQ=255)
                                   ggATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGt                                                                                                                >  1:696895/1‑151 (MQ=255)
                                     atatCAGCGTCAGGATACACCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTa                                                                                                               <  1:741965/150‑1 (MQ=255)
                                      tatCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATc                                                                                                             >  2:934178/1‑151 (MQ=255)
                                        tCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGt                                                                                                           >  2:896455/1‑151 (MQ=255)
                                         cAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGt                                                                                                           >  1:585517/1‑150 (MQ=255)
                                          aGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTa                                                                                                          >  2:103437/1‑150 (MQ=255)
                                             gTCAGGATACATCTTAATGAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAgc                                                                                                      <  2:787963/151‑1 (MQ=255)
                                             gTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAgc                                                                                                      >  2:488688/1‑151 (MQ=255)
                                               cAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAgcgc                                                                                                    >  2:253198/1‑151 (MQ=255)
                                               cAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAgcg                                                                                                     <  2:270159/150‑1 (MQ=255)
                                                   aTACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGc                                                                                                >  1:645106/1‑151 (MQ=255)
                                                        tCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGa                                                                                            >  1:1142201/1‑150 (MQ=255)
                                                         cTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCGAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGAActgcggctgcgg                                                                                              >  1:997144/1‑147 (MQ=255)
                                                         cTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGa                                                                                            >  2:682322/1‑149 (MQ=255)
                                                          ttAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGc                                                                                         >  2:977372/1‑151 (MQ=255)
                                                           tAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGc                                                                                         <  2:181799/150‑1 (MQ=255)
                                                            aaTCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGcc                                                                                        <  1:527632/150‑1 (MQ=255)
                                                              tCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGccacc                                                                                     >  1:96850/1‑151 (MQ=255)
                                                               cAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGccacc                                                                                     <  1:196415/150‑1 (MQ=255)
                                                                aGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCaa                                                                                   <  1:998998/151‑1 (MQ=255)
                                                                     gCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTc                                                                               >  1:742395/1‑150 (MQ=255)
                                                                      ccccACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAACCACCAAAGTCAt                                                                             >  2:609808/1‑151 (MQ=255)
                                                                       cccACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATg                                                                            >  2:883023/1‑151 (MQ=255)
                                                                       cccACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATg                                                                            <  1:228471/151‑1 (MQ=255)
                                                                       cccACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATg                                                                            >  1:347802/1‑151 (MQ=255)
                                                                        ccACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGa                                                                           <  2:945964/151‑1 (MQ=255)
                                                                         cACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAAGGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGa                                                                           <  2:1140428/150‑1 (MQ=255)
                                                                           cGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATc                                                                        >  2:864013/1‑151 (MQ=255)
                                                                           cGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATc                                                                        >  1:312648/1‑151 (MQ=255)
                                                                             aGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCg                                                                       >  2:87890/1‑150 (MQ=255)
                                                                                 gTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGggc                                                                  >  1:362048/1‑151 (MQ=255)
                                                                                   ggTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGggcgg                                                                >  2:58527/1‑151 (MQ=255)
                                                                                     tCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGgcggcg                                                              >  1:808364/1‑151 (MQ=255)
                                                                                      cAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGt                                                             <  2:525174/151‑1 (MQ=255)
                                                                                          gCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTcc                                                         <  2:456007/151‑1 (MQ=255)
                                                                                             ccAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATc                                                      <  2:923230/151‑1 (MQ=255)
                                                                                               aGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATcgc                                                    >  2:137170/1‑151 (MQ=255)
                                                                                                ggCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATcgcg                                                   >  2:1122590/1‑151 (MQ=255)
                                                                                                 gCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCgg                                                  >  2:106511/1‑151 (MQ=255)
                                                                                                    tcttTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGt                                                 <  1:940757/149‑1 (MQ=255)
                                                                                                     cttTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCAc                                              >  2:770826/1‑151 (MQ=255)
                                                                                                              gCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTcc                                     <  2:549462/151‑1 (MQ=255)
                                                                                                              gCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTcc                                     >  1:641052/1‑151 (MQ=255)
                                                                                                               cGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTcc                                     >  1:209655/1‑150 (MQ=255)
                                                                                                                gTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCAc                                   >  1:968485/1‑151 (MQ=255)
                                                                                                                  cACCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACgg                                 >  1:606129/1‑151 (MQ=255)
                                                                                                                   aCCGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAGCCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCTTGGGCGGCGTCGCCATCGCTGTCACTCCTGTTCCACgg                                 <  2:954209/150‑1 (MQ=255)
                                                                                                                    ccGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGt                               >  1:716004/1‑151 (MQ=255)
                                                                                                                     cGGGATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTa                              <  2:666361/151‑1 (MQ=255)
                                                                                                                        gATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAAc                           >  1:567233/1‑151 (MQ=255)
                                                                                                                        gATCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAAc                           >  2:275692/1‑151 (MQ=255)
                                                                                                                          tCGGCGCTAACAGCTCAACCAGACGCGGTGCCAGAGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAAcc                          >  2:73272/1‑150 (MQ=255)
                                                                                                                            ggCGCTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGt                       >  1:799774/1‑151 (MQ=255)
                                                                                                                              cgcTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTg                      >  1:298709/1‑150 (MQ=255)
                                                                                                                              cgcTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGa                     <  2:347788/151‑1 (MQ=255)
                                                                                                                               gcTAACAGCTCAACCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAg                    <  1:387323/151‑1 (MQ=255)
                                                                                                                                          aaCCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAaccacc         >  2:455263/1‑151 (MQ=255)
                                                                                                                                          aaCCAGACGCGGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAaccacc         <  2:522216/151‑1 (MQ=255)
                                                                                                                                                 cgcgGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCg  <  1:1025221/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCG  >  NC_000913/3181991‑3182286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑