Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,213,290	C→A	Q82K (CAG→AAG)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,213,290	0	C	A	100.0%	330.2 / NA	84	Q82K (CAG→AAG)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (59/25);  total (59/25)

AAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGG  >  NC_000913/3213144‑3213434                                                                                                                                                   |                                                                                                                                                 aaGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAgg                                                                                                                                               >  2:522605/1‑150 (MQ=255)  aGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTg                                                                                                                                             <  2:132633/151‑1 (MQ=255)   gATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGc                                                                                                                                            >  1:736961/1‑151 (MQ=255)      atCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCttt                                                                                                                                         >  1:892882/1‑151 (MQ=255)       tcgtcgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTc                                                                                                                                        >  1:157304/1‑151 (MQ=255)         gtcgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTcc                                                                                                                                       >  1:736243/1‑150 (MQ=255)           cgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGc                                                                                                                                    >  1:353708/1‑151 (MQ=255)            gATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCg                                                                                                                                   >  1:405096/1‑151 (MQ=255)            gATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCg                                                                                                                                   >  2:1069591/1‑151 (MQ=255)             aTTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGt                                                                                                                                  >  1:1197100/1‑151 (MQ=255)             aTTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGt                                                                                                                                  >  2:430628/1‑151 (MQ=255)               tcagatcagaTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTg                                                                                                                                 <  1:1167473/150‑1 (MQ=255)                cagatcagatCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTgg                                                                                                                                <  1:1034989/150‑1 (MQ=255)                cagatcagatCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTgg                                                                                                                                <  1:288745/150‑1 (MQ=255)                 agatcagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGa                                                                                                                               >  2:39600/1‑150 (MQ=255)                     cagatcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCt                                                                                                                           <  1:723986/150‑1 (MQ=255)                        atcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTg                                                                                                                          >  2:850645/1‑148 (MQ=255)                        atcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGaa                                                                                                                        >  1:548493/1‑150 (MQ=255)                        atcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGATGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGaa                                                                                                                        >  2:656511/1‑150 (MQ=255)                          cGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATc                                                                                                                     >  2:526659/1‑151 (MQ=255)                           gAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCg                                                                                                                    >  2:522504/1‑151 (MQ=255)                           gAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCCTTCCAGCGTGGAATCTGAAATCg                                                                                                                    >  1:850782/1‑151 (MQ=255)                              gACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGgc                                                                                                                 >  1:1099207/1‑151 (MQ=255)                                    atcCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACg                                                                                                            >  1:108406/1‑150 (MQ=255)                                      ccAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACt                                                                                                         >  2:927688/1‑151 (MQ=255)                                        aaaTGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTg                                                                                                        >  2:1134562/1‑150 (MQ=255)                                        aaaTGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTg                                                                                                        >  1:1180322/1‑150 (MQ=255)                                          aTGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGAcc                                                                                                     >  2:260098/1‑151 (MQ=255)                                             aTCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCgg                                                                                                  >  2:1200860/1‑151 (MQ=255)                                             aTCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCgg                                                                                                  >  1:1022427/1‑151 (MQ=255)                                             aTCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCgg                                                                                                  >  1:488722/1‑151 (MQ=255)                                             aTCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCg                                                                                                   <  1:1001437/150‑1 (MQ=255)                                                  cGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGc                                                                                             >  2:37458/1‑151 (MQ=255)                                                   gACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGc                                                                                             >  1:286342/1‑150 (MQ=255)                                                      aTGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGt                                                                                         <  1:56685/151‑1 (MQ=255)                                                      aTGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGt                                                                                         >  1:446486/1‑151 (MQ=255)                                                          gCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACAt                                                                                     >  2:1088817/1‑151 (MQ=255)                                                           cATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGAGGCTGGCTGAAAACACCGCGGAAGAAGATTCTGCCGAAGCCGCCGCGAAGTTGCTTTCCAGCGTGGAATCTGAACTCGGGCGCACGACTGACCCGGTACGCATGTACAt                                                                                     >  2:260585/1‑150 (MQ=255)                                                            aTTCAGGCGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGc                                                                                   >  2:350718/1‑151 (MQ=255)                                                               cAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTg                                                                                >  2:954263/1‑151 (MQ=255)                                                                    gATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGCCCCGGTACGCATGTACATGCGTGAAAt                                                                            <  1:909690/150‑1 (MQ=255)                                                                    gATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAAt                                                                            <  1:1049971/150‑1 (MQ=255)                                                                      tGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATggg                                                                         >  1:852305/1‑151 (MQ=255)                                                                        gaagaaGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGc                                                                        >  1:539404/1‑150 (MQ=255)                                                                          agaagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc                                                                     >  2:142438/1‑151 (MQ=255)                                                                          agaagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATATGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc                                                                     >  2:785839/1‑151 (MQ=255)                                                                            aagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGt                                                                   <  2:616159/151‑1 (MQ=255)                                                                             agCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGtt                                                                  <  1:306982/151‑1 (MQ=255)                                                                               cACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGa                                                                <  2:838404/151‑1 (MQ=255)                                                                                  cGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt                                                             >  2:390996/1‑151 (MQ=255)                                                                                   ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTg                                                            >  2:375932/1‑151 (MQ=255)                                                                                   ggATGCCGATGATCGGATGCGGGCTGAAAACCCCGCGGAGGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGAAGGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt                                                             <  2:209554/150‑1 (MQ=255)                                                                                     aTGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGtt                                                          >  2:1054904/1‑151 (MQ=255)                                                                                       gCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGa                                                        <  2:548468/151‑1 (MQ=255)                                                                                        ccGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGAc                                                       <  1:22871/151‑1 (MQ=255)                                                                                         cGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGAcc                                                      >  2:1017849/1‑151 (MQ=255)                                                                                             gatCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg                                                  >  1:2401/1‑151 (MQ=255)                                                                                               tCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGACGGGGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg                                                  >  1:33550/1‑149 (MQ=255)                                                                                               tCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                 >  2:106925/1‑150 (MQ=255)                                                                                                cTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                 >  1:21231/1‑149 (MQ=255)                                                                                                 tGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGaa                                                >  2:718176/1‑149 (MQ=255)                                                                                                  gATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGaa                                                <  2:99077/148‑1 (MQ=255)                                                                                                  gATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAgg                                              <  1:1201797/150‑1 (MQ=255)                                                                                                    tGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGc                                             >  2:1190563/1‑149 (MQ=255)                                                                                                    tGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCg                                            >  2:786940/1‑150 (MQ=255)                                                                                                      ctggctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaa                                          >  1:655773/1‑150 (MQ=255)                                                                                                             aaaaCACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGAc                                    <  2:560267/149‑1 (MQ=255)                                                                                                                 cacCGCGGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCt                              <  1:587141/151‑1 (MQ=255)                                                                                                                    cgcgGACGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg                           <  1:615702/151‑1 (MQ=255)                                                                                                                          cGAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAt                      <  2:99898/150‑1 (MQ=255)                                                                                                                           gAAGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg                    >  1:1111579/1‑151 (MQ=255)                                                                                                                            aaGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg                    >  1:592771/1‑150 (MQ=255)                                                                                                                            aaGATGCTCCCGAACCCGCCGAGAAGGTGCTTTCCAGCGTGGCATCTGCAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGCACTGTTGACCCGCGCAGGCGAAATTGACATCGCTAAGCGTATTg                    >  2:1063440/1‑150 (MQ=255)                                                                                                                             aGATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGaa                  <  2:1072895/151‑1 (MQ=255)                                                                                                                              gATGCTGCCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAg                 <  1:1207627/151‑1 (MQ=255)                                                                                                                                 gctgcCGAGGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg              <  2:113962/151‑1 (MQ=255)                                                                                                                                   tgcCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACggg            >  1:556281/1‑151 (MQ=255)                                                                                                                                    gcCGAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACggg            >  1:1187761/1‑150 (MQ=255)                                                                                                                                       gAAGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc         >  1:738875/1‑150 (MQ=255)                                                                                                                                        aaGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa        >  1:282715/1‑150 (MQ=255)                                                                                                                                        aaGCCGCCGCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa        >  1:638076/1‑150 (MQ=255)                                                                                                                                           ccgccgCGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa       <  2:269365/148‑1 (MQ=255)                                                                                                                                            cgccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc     >  1:969299/1‑149 (MQ=255)                                                                                                                                             gccgcGAAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAgg  >  1:356617/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGG  >  NC_000913/3213144‑3213434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑