Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,331,811	T→G	K309Q (AAG→CAG)	yhbE ←	EamA family inner membrane putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,331,811	0	T	G	100.0%	355.5 / NA	91	K309Q (AAG→CAG)	yhbE	EamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (47/44);  total (47/44)

CGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACA  >  NC_000913/3331665‑3331957                                                                                                                                                   |                                                                                                                                                   cGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATTGATGCTTCATCAACAAACTTCCTTTTATTCTCCGTAAATCATTCGCCTGCTCGGGGTGGCGATACCACCGTTGTATGCTGACGt                                                                                                                                                >  2:231734/1‑151 (MQ=255) cGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGt                                                                                                                                                >  1:341005/1‑151 (MQ=255)         aCTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGcc                                                                                                                                        >  2:15017/1‑151 (MQ=255)           ttttCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGcccc                                                                                                                                      >  2:638906/1‑151 (MQ=255)             ttCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCa                                                                                                                                     <  1:1099188/150‑1 (MQ=255)              tCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCaa                                                                                                                                    <  1:225351/150‑1 (MQ=255)               cgcgGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAAt                                                                                                                                  >  1:1181611/1‑151 (MQ=255)                 cggcggAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATAc                                                                                                                                >  2:441229/1‑151 (MQ=255)                           tcacgacgccagtgccGCCATCCCCTTCAACGACCAGAATCGATGCTTCATCAACAACCTGCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATTCTGACGTAATCCGCCCCAAATACGATGa                                                                                                                           <  2:1041700/132‑1 (MQ=255)                           tCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCaa                                                                                                                       >  2:958419/1‑150 (MQ=255)                           tCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAAt                                                                                                                      >  1:1132929/1‑151 (MQ=255)                            cACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCaa                                                                                                                       <  1:624867/149‑1 (MQ=255)                             aCGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATgg                                                                                                                    >  1:1095201/1‑151 (MQ=255)                               gCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCg                                                                                                                  >  1:715699/1‑151 (MQ=255)                                 aaCCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGa                                                                                                                <  1:246839/151‑1 (MQ=255)                                   ccATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAAt                                                                                                              <  1:637556/151‑1 (MQ=255)                                   ccATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAAt                                                                                                              >  2:139033/1‑151 (MQ=255)                                     aTTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATa                                                                                                             <  2:39695/150‑1 (MQ=255)                                           gCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATcgcg                                                                                                      >  2:166130/1‑151 (MQ=255)                                            ccATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCgcgc                                                                                                     >  1:394585/1‑151 (MQ=255)                                                cACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGc                                                                                                 >  1:491886/1‑151 (MQ=255)                                                  ccTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCa                                                                                                <  2:988366/150‑1 (MQ=255)                                                      cAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCAc                                                                                           <  2:889640/151‑1 (MQ=255)                                                      cAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCAc                                                                                           >  2:193702/1‑151 (MQ=255)                                                       aaCGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACg                                                                                          <  1:1083293/151‑1 (MQ=255)                                                        aCGACCATAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGa                                                                                         <  2:1210072/151‑1 (MQ=255)                                                            ccAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACaa                                                                                      >  1:232605/1‑150 (MQ=255)                                                              aGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACg                                                                                   >  1:1027108/1‑151 (MQ=255)                                                               gAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGc                                                                                  >  2:453627/1‑151 (MQ=255)                                                                  tCGATGCTTCATCAACAAACTTCATTTTAGTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGGTGACGTAAACCGCCCCAAATACGAAGACCAAAGGGGTAATACATCGCGCCCGCAACCACGCCAACCGCa                                                                                 <  2:528128/149‑1 (MQ=255)                                                                   cGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGAAGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCAcc                                                                               <  1:712185/150‑1 (MQ=255)                                                                      tGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCgag                                                                            <  2:979997/150‑1 (MQ=255)                                                                          tcatcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAAc                                                                       >  2:401357/1‑151 (MQ=255)                                                                           catcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAAcc                                                                      <  1:1083733/151‑1 (MQ=255)                                                                           catcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAAc                                                                       <  1:803562/150‑1 (MQ=255)                                                                           catcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAAc                                                                       <  1:1076131/150‑1 (MQ=255)                                                                           catcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAAc                                                                       <  1:532969/150‑1 (MQ=255)                                                                             tcaACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCt                                                                     >  2:673246/1‑150 (MQ=255)                                                                              caacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTaa                                                                   <  2:1209812/151‑1 (MQ=255)                                                                              caacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTa                                                                    >  2:57139/1‑150 (MQ=255)                                                                              caacaaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTa                                                                    >  2:833801/1‑150 (MQ=255)                                                                                 caaACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAg                                                                <  1:530169/151‑1 (MQ=255)                                                                                  aaaCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAgg                                                               >  2:116678/1‑151 (MQ=255)                                                                                  aaaCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAgg                                                               <  1:34556/151‑1 (MQ=255)                                                                                   aaCTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGt                                                              >  2:850181/1‑151 (MQ=255)                                                                                       tCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGttt                                                            <  2:780117/149‑1 (MQ=255)                                                                                        cATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTaa                                                          <  2:582716/150‑1 (MQ=255)                                                                                        cATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAAc                                                         >  2:967835/1‑151 (MQ=255)                                                                                        cATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAAc                                                         >  1:176650/1‑151 (MQ=255)                                                                                         aTTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAAc                                                         >  1:1057895/1‑150 (MQ=255)                                                                                         aTTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAAc                                                         >  2:149414/1‑150 (MQ=255)                                                                                          ttttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACAt                                                       >  2:860928/1‑151 (MQ=255)                                                                                           tttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACAt                                                       <  2:469948/150‑1 (MQ=255)                                                                                            ttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCg                                                     >  2:548777/1‑151 (MQ=255)                                                                                            ttATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCg                                                     >  2:302233/1‑151 (MQ=255)                                                                                              aTTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGt                                                   <  1:601641/151‑1 (MQ=255)                                                                                              aTTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGt                                                   <  2:389440/151‑1 (MQ=255)                                                                                                tctcCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTc                                                  <  2:1131357/150‑1 (MQ=255)                                                                                                 ctcCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTg                                                >  1:233186/1‑151 (MQ=255)                                                                                                   ccGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTgg                                               <  1:769209/150‑1 (MQ=255)                                                                                                   ccGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTg                                                <  1:1170348/149‑1 (MQ=255)                                                                                                     gTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCg                                             >  2:767560/1‑150 (MQ=255)                                                                                                      tAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCga                                            >  1:745705/1‑150 (MQ=255)                                                                                                        aaTCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCga                                            >  2:379039/1‑148 (MQ=255)                                                                                                            aTTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATc                                     >  2:725227/1‑151 (MQ=255)                                                                                                              tCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCgg                                   >  1:323738/1‑151 (MQ=255)                                                                                                               cGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCggg                                  >  2:750736/1‑151 (MQ=255)                                                                                                                 ccTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcc                                >  1:1091785/1‑151 (MQ=255)                                                                                                                  cTGCGCGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcc                                >  1:916102/1‑150 (MQ=255)                                                                                                                    gcgcGGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggcca                               <  1:1085944/149‑1 (MQ=255)                                                                                                                     cgcgGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggccaggc                            >  1:251753/1‑151 (MQ=255)                                                                                                                      gcgGGGTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGggccaggcc                           >  1:355841/1‑151 (MQ=255)                                                                                                                       cgcggTTGCGATGCCTCCGTTGGATGATGACGCAATACACCACAGCTACGATAACCAATGGCGTAATTCATCGCGCCTGCAACGAAGACGACCGTGCCGAGATAGCCCATAAGGTTTAACATCGGTCTGGCGAAGAAAACGtgccagagc                           <  2:804116/147‑4 (MQ=255)                                                                                                                         gggTTGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGt                        <  2:770243/151‑1 (MQ=255)                                                                                                                           gTTGCGATCCCCCCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATACCCTAAAAGGTTTAACATTGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGa                      <  1:1211480/151‑1 (MQ=255)                                                                                                                             tGCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTg                       >  2:290095/1‑148 (MQ=255)                                                                                                                              gCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaa                   <  2:1024829/151‑1 (MQ=255)                                                                                                                              gCGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATa                    >  1:902574/1‑150 (MQ=255)                                                                                                                               cGATACCACCGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaaa                  <  2:969001/151‑1 (MQ=255)                                                                                                                                 aTACCACCGGTGTATGCTGACGTAATCCGCCCCAAATACGATGTCCAATGGCGGAATACATCGCGCCCACAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCTGGCGAGTGATaaa                  <  2:17814/149‑1 (MQ=255)                                                                                                                                  tACCACCGTTGTATGCTGAAGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAg                <  2:185969/150‑1 (MQ=255)                                                                                                                                    ccaccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaaa                  >  2:588905/1‑146 (MQ=255)                                                                                                                                    ccaccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATaaa                  >  2:14757/1‑146 (MQ=255)                                                                                                                                    ccaccGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATc             <  1:273784/151‑1 (MQ=255)                                                                                                                                        cGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaa         <  1:426225/151‑1 (MQ=255)                                                                                                                                        cGTTGTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaa         <  1:131432/151‑1 (MQ=255)                                                                                                                                            gTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaa      >  1:45452/1‑150 (MQ=255)                                                                                                                                            gTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaa      <  2:253952/150‑1 (MQ=255)                                                                                                                                            gTATGCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGAGCTGaaaaa      <  2:249731/150‑1 (MQ=255)                                                                                                                                            gTATGCTGACGTAATCCGCCCAAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCATCGAGATAGCCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaa      <  2:1129199/150‑1 (MQ=255)                                                                                                                                                gCTGACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACa  <  2:582295/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGGAATATACTTTTCGCGGCGGAAGCTCACGCAACCATTACCGCCATCACCTGCAACGACCAGAATCGATGCTTCATCAACAAACTTCATTTTATTCTCCGTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACA  >  NC_000913/3331665‑3331957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑